Reviewed, UniProtKB/Swiss-Prot Q28C69 (ENOPH_XENTR)

Names and origin

Protein names

Recommended name:
    Enolase-phosphatase E1
    EC=3.1.3.77
Alternative name(s):
    2,3-diketo-5-methylthio-1-phosphopentane phosphatase
    MASA homolog

Gene names

Name:	enoph1
Synonyms:	masa
ORF Names:	TTpA008k20.1

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Bifunctional enzyme that enolizes the substrate to form the intermediate 2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate, which is then dephosphorylated to form the acireductone 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one By similarity.
Catalytic activity	5-(methylthio)-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6. Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. MasA/mtnC family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine salvage Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	acireductone synthase activity Inferred from sequence or structural similarity. Source: UniProtKB magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycolate phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	Enolase-phosphatase E1		PRO_0000254011
Regions
	Region	151 – 152	2	Substrate binding By similarity
Sites
	Metal binding	16	1	Magnesium By similarity
	Metal binding	18	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	210	1	Magnesium By similarity
	Binding site	185	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q28C69-1 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: F3D704FAF3DE8728	FASTA	259	29,184
	10 20 30 40 50 60 MALFSVPPPV TVILLDIEGT TTPITFVKDV LFPYVKENIK KYLLEHWQEK ECQEDVTQLQ 70 80 90 100 110 120 KQAEKDSHLD GFVPIPSGVS DNTTEHMIQA VVDNVYWQMS FDRKTTALKQ LQGHMWRSAY 130 140 150 160 170 180 ISGQLKGEVY EDVVPSIRQW RELGIKLYIY SSGSIDAQKL LFGYSIEGDL LKLLDGHFDT 190 200 210 220 230 240 NIGHKVESKS YRNIADNIGC LPENILFLTD VVKEALAAEK AGLHVAVVVR PGNAALTDED 250 KSNCCCITSF HQIHFPSQK

References

[1]	Sanger Xenopus tropicalis EST/cDNA project Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Tadpole.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR942427 mRNA. Translation: CAJ83209.1. BC118728 mRNA. Translation: AAI18729.1.
RefSeq	NP_001039112.1.
UniGene	Str.52196
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q28C69.
Genome annotation databases
GeneID	733933.
KEGG	xtr:733933.
Organism-specific databases
CTD	733933.
Xenbase	XB-FEAT-954013. enoph1.
Phylogenomic databases
HOVERGEN	Q28C69.
Enzyme and pathway databases
BRENDA	3.1.3.77. 279072.
Family and domain databases
InterPro	IPR005834. Dehalogen-like_hydro. IPR010041. Enolase_ppase. IPR006439. HAD-SF_hydro_IA_v1. [Graphical view]
PANTHER	PTHR20371. Enolase_ppase. 1 hit.
Pfam	PF00702. Hydrolase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01691. enolase-ppase. 1 hit. TIGR01549. HAD-SF-IA-v1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ENOPH_XENTR

Accession

Primary (citable) accession number: Q28C69

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	April 4, 2006
Last modified:	October 13, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project