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Protein

POU domain, class 2, transcription factor 1

Gene

pou2f1

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and histone H2B. Acts downstream of Notch signaling during radial glia formation. Regulates apoptosis, possibly via an FGF-signaling pathway (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi395 – 45460HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_290816. RNA Polymerase III Transcription Initiation From Type 3 Promoter.
REACT_336102. RNA Polymerase III Abortive And Retractive Initiation.

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 2, transcription factor 1Imported
Alternative name(s):
Octamer-binding protein 1
Short name:
Oct-1By similarity
Octamer-binding transcription factor 1By similarity
Short name:
OTF-1
Gene namesi
Name:pou2f1Imported
Synonyms:oct-1By similarity, oct1By similarity
ORF Names:TEgg001p15.1
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
ProteomesiUP000008143 Componenti: Unassembled WGS sequence

Organism-specific databases

XenbaseiXB-GENE-853992. pou2f1.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus PROSITE-ProRule annotationBy similarity

  • Note: Retained in the cytoplasm during early development, then gradually translocates to the nucleus around the mid-blastula transition (MBT).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 757757POU domain, class 2, transcription factor 1PRO_0000389618Add
BLAST

Expressioni

Gene expression databases

BgeeiQ28BL7.
ExpressionAtlasiQ28BL7. baseline.

Interactioni

Protein-protein interaction databases

STRINGi8364.ENSXETP00000013682.

Structurei

3D structure databases

ProteinModelPortaliQ28BL7.
SMRiQ28BL7. Positions 295-455.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini294 – 36875POU-specificPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi47 – 282236Gln-richSequence AnalysisAdd
BLAST
Compositional biasi479 – 591113Thr-richSequence AnalysisAdd
BLAST

Domaini

The POU domain controls nuclear translocation.By similarity

Sequence similaritiesi

Belongs to the POU transcription factor family. Class-2 subfamily.Sequence Analysis
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG267922.
HOGENOMiHOG000220842.
HOVERGENiHBG057016.
InParanoidiQ28BL7.
KOiK09364.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR000972. TF_octamer.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00029. OCTAMER.
PR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q28BL7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLHSSSKIP NHAWLSDARM NNPSETSKSS ESGDGNTGTQ TNGLDFQKQA
60 70 80 90 100
VPIGAITSAQ AQALLGHLHQ VQLAGTSLQA AAQSLNVQTK FKEEPGEPTQ
110 120 130 140 150
AVQPSQQPSL QAAIPQTQLM VAGGPITGLT LTPAQQQLLL QQAQAQLLAA
160 170 180 190 200
AVQHSASQQH SAAGATISAS AATPMTQIPL SQPIQIAQDL QQLQQLQQQN
210 220 230 240 250
LNLQQFVLVH PTTNLPPAQF IISQTPQGQQ GLLQAQNLLT QLPQQSQANL
260 270 280 290 300
LQSQPSITLT SQPATPTRTI AATPVQQLPQ SQTTPKRIDT PSLEEPSDLE
310 320 330 340 350
ELEQFAKTFK QRRIKLGFTQ GDVGLAMGKL YGNDFSQTTI SRFEALNLSF
360 370 380 390 400
KNMCKLKPLL EKWLNDAENI TSDSSLSNQS VLNSPGHGME GLNRRRKKRT
410 420 430 440 450
SIETNIRVAL EKSFLENQKP TSEEITMIAD QLNMEKEVIR VWFCNRRQKE
460 470 480 490 500
KRINPPSSGG SSSSPIKSLF SSANPLVATT PSLVTSSTAT TLTVNPVLPL
510 520 530 540 550
TSAAAITSFP VPGTTGTSSA NTATVISTAP PVSSVLTSPS LSPSPSATAA
560 570 580 590 600
TSEASTASGT STTHTTSTPL TSPLSTGQVM VTASGIHTAA TALQGAAQLP
610 620 630 640 650
TSASLAAMAA AAGLNPGLMA PSQFAAGGAL FSLNPGALGS ALSPALMSNS
660 670 680 690 700
TLATIQALAS SGSLPITSLD ATGNLVFANA GGTPNIVTAP LFLNPQNLSL
710 720 730 740 750
LTSNPVSLVS AASTGVTGPI TSLHATTSSV DSVQNTLFTV ASASGAASTT

TSASKAQ
Length:757
Mass (Da):78,444
Last modified:April 4, 2006 - v1
Checksum:i65AD8A5AF51B02E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR942771 mRNA. Translation: CAJ82126.1.
RefSeqiNP_001016337.2. NM_001016337.3.
UniGeneiStr.48304.

Genome annotation databases

GeneIDi549091.
KEGGixtr:549091.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR942771 mRNA. Translation: CAJ82126.1.
RefSeqiNP_001016337.2. NM_001016337.3.
UniGeneiStr.48304.

3D structure databases

ProteinModelPortaliQ28BL7.
SMRiQ28BL7. Positions 295-455.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi8364.ENSXETP00000013682.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi549091.
KEGGixtr:549091.

Organism-specific databases

CTDi5451.
XenbaseiXB-GENE-853992. pou2f1.

Phylogenomic databases

eggNOGiNOG267922.
HOGENOMiHOG000220842.
HOVERGENiHBG057016.
InParanoidiQ28BL7.
KOiK09364.

Enzyme and pathway databases

ReactomeiREACT_290816. RNA Polymerase III Transcription Initiation From Type 3 Promoter.
REACT_336102. RNA Polymerase III Abortive And Retractive Initiation.

Gene expression databases

BgeeiQ28BL7.
ExpressionAtlasiQ28BL7. baseline.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR000972. TF_octamer.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00029. OCTAMER.
PR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Sanger Xenopus tropicalis EST/cDNA project
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: EggImported.

Entry informationi

Entry nameiPO2F1_XENTR
AccessioniPrimary (citable) accession number: Q28BL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: April 4, 2006
Last modified: May 27, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.