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Q28983

- ZAN_PIG

UniProt

Q28983 - ZAN_PIG

Protein

Zonadhesin

Gene

ZAN

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Binds in a species-specific manner to the zona pellucida of the egg. May be involved in gamete recognition and/or signaling.

    GO - Molecular functioni

    1. extracellular matrix binding Source: MGI

    GO - Biological processi

    1. binding of sperm to zona pellucida Source: MGI
    2. cell adhesion Source: UniProtKB-KW

    Keywords - Biological processi

    Cell adhesion

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zonadhesin
    Gene namesi
    Name:ZAN
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Unplaced

    Subcellular locationi

    Cell membrane; Single-pass type I membrane protein
    Note: Exclusively on the apical region of the sperm head.By similarity

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Sequence AnalysisAdd
    BLAST
    Chaini30 – 24762447ZonadhesinPRO_0000007785Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi109 – 1091N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi269 – 2691N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi735 – 7351N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi758 – 7581N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi833 – 8331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1154 – 11541N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1329 – 13291N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1448 – 14481N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1544 – 15441N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1596 – 15961N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1654 – 16541N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1843 – 18431N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1965 – 19651N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi2122 – 21221N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi2165 – 21651N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi2178 – 21781N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi2329 – 23291N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi2359 – 23591N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi2370 ↔ 2381By similarity
    Disulfide bondi2375 ↔ 2390By similarity
    Disulfide bondi2392 ↔ 2401By similarity

    Post-translational modificationi

    The MAM domains and the mucin-like domains are missing from the zonadhesin that binds to the egg extracellular matrix. Processing might occur during sperm maturation and/or capacitation.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ28983.

    Expressioni

    Tissue specificityi

    In testis, primarily in haploid spermatids. Not in lung, liver, heart, spleen, brain, kidney, epididymis.

    Interactioni

    Subunit structurei

    Probably forms covalent oligomers.

    Protein-protein interaction databases

    STRINGi9823.ENSSSCP00000008195.

    Structurei

    3D structure databases

    ProteinModelPortaliQ28983.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini30 – 24182389ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini2440 – 247637CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei2419 – 243921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini31 – 144114MAM 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini147 – 312166MAM 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini690 – 73950TIL 1Add
    BLAST
    Domaini740 – 79455VWFC 1Add
    BLAST
    Domaini800 – 1015216VWFD 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini1070 – 112354TIL 2Add
    BLAST
    Domaini1124 – 118057VWFC 2Add
    BLAST
    Domaini1185 – 1402218VWFD 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini1456 – 151156TIL 3Add
    BLAST
    Domaini1512 – 156857VWFC 3Add
    BLAST
    Domaini1574 – 1784211VWFD 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini1851 – 190757TIL 4Add
    BLAST
    Domaini1908 – 196356VWFC 4Add
    BLAST
    Domaini1969 – 2172204VWFD 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini2257 – 231054TIL 5Add
    BLAST
    Domaini2311 – 236555VWFC 5Add
    BLAST
    Domaini2366 – 240237EGF-likePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni319 – 68736953 X approximate heptapeptide repeats (mucin-like domain)Add
    BLAST

    Domaini

    The MAM domains probably mediate sperm adhesion to the zona pellucida.
    During sperm migration through the reproductive tracts, the mucin-like domain might inhibit inappropriate trapping of spermatozoa or promoting adhesion to the oviductal isthmus.
    The VWFD domains 2 and 3 may mediate covalent oligomerization (By similarity to human intestinal mucin MUC2).

    Sequence similaritiesi

    Contains 1 EGF-like domain.PROSITE-ProRule annotation
    Contains 2 MAM domains.PROSITE-ProRule annotation
    Contains 5 VWFC domains.Curated
    Contains 4 VWFD domains.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG12793.
    HOGENOMiHOG000168448.
    HOVERGENiHBG000375.

    Family and domain databases

    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR000998. MAM_dom.
    IPR002919. TIL_dom.
    IPR025615. TILa_dom.
    IPR014853. Unchr_dom_Cys-rich.
    IPR001007. VWF_C.
    IPR001846. VWF_type-D.
    [Graphical view]
    PfamiPF08742. C8. 4 hits.
    PF00008. EGF. 1 hit.
    PF00629. MAM. 2 hits.
    PF01826. TIL. 5 hits.
    PF12714. TILa. 5 hits.
    PF00094. VWD. 4 hits.
    [Graphical view]
    SMARTiSM00832. C8. 4 hits.
    SM00181. EGF. 1 hit.
    SM00137. MAM. 1 hit.
    SM00214. VWC. 2 hits.
    SM00216. VWD. 4 hits.
    [Graphical view]
    SUPFAMiSSF49899. SSF49899. 2 hits.
    SSF57567. SSF57567. 5 hits.
    PROSITEiPS00022. EGF_1. 1 hit.
    PS01186. EGF_2. 4 hits.
    PS50026. EGF_3. 1 hit.
    PS00740. MAM_1. 1 hit.
    PS50060. MAM_2. 2 hits.
    PS51233. VWFD. 4 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q28983-1 [UniParc]FASTAAdd to Basket

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    MLGLPALAGP MAMPHPPLIP STPTLLAFSF PGGFYMLLDP KNAKPRQRSA     50
    LLSPLIQSSG CLSLSFQYTQ RGQASGATLM VYASVLGSIR KHTLFSGQPG 100
    PSWQPVSVNY TSQGQIQFTL VGVFGKIPEP AVAVDAISIA PCEESFPQCD 150
    FEDNAHPFCD WVQASQDGGY WRQGNKNTFI QPAGPFGISL NGEGHYIFLE 200
    TDKFSQAGQS FRLVSRPFCA PAVICVTFTY HMYGLGQGTK LRLLLGSPAG 250
    SPPSSLWERV GPQSPEWLNT SVTIPSGHQQ PMQLIFEAVR GTNTAFVVAL 300
    GFVLINHGTC RGPSETSVST EKPVAPTEKP TVPSEIYTIP TEKPMVHMEK 350
    PIVHTEKPTV PTEKPTIPTE KSTVPTKKPT VFKEPTLPPE GPTVPAERPT 400
    TPPEGPAVPP KGPTVLTEWP TSHTEKSTVH TEKPILPTGK STIPTEKPMV 450
    PTKRTTTPTE RTTIPAEKPT VPIEKPMVPT ERTTIPTERT TIPTEKPTVP 500
    TEKLTVPTEK PIVPTEKPIV PTEKHTIPTE KLTVLTERTT TPTERTTIPT 550
    EKPTVPTEKP SVPTEKPTVP TEEPTIPTEK LTVPTERTTT PTKRTTTPTI 600
    RTTTPTIRTT TPTERTTTPT IRTTTPTERT TIPTKKTTVP TEKTIIPTER 650
    TIAPTTPQPS PTLVPTQPAA VVMPSTSATT VTPRTTIASC PPNAHFERCA 700
    CPVSCQSPTP NCELFCKPGC VCDPGFLFSG SHCVNASSCD CFYNDNYYKL 750
    GTDWFSPNCT EHCHCRPSSR MECQTFKCGT HTVCQLKNGQ YGCHPYGSAT 800
    CSVYGDPHYL TFDGRRFNFM GKCTYILAQP CGNLTEHFFR VLVKKEERGQ 850
    EGVSCLSKVY VTLPESTVTL LKGRHTLVGG QRVTLPAIPS RGVFLAPSGR 900
    FVELQTAFGL RVRWDGDQQL FVSVPSTFSG KLCGLCGDYD GDSSNDNQKP 950
    DGSPAKDEKE LGSSWQTSED ADQQCEENQV SPPSCNTALQ NTMSGPEFCG 1000
    QLVAPHGVFE ACLPHLRASS FFKSCTFDMC NFQGLQHMLC AHMSALTENC 1050
    QDAGYTVKPW RGPQFCPLAC PRNSRYTLCA RLCPDTCHSE FSGRACKDRC 1100
    VEGCECDPGF VLSGLQCVSR SECGCLDSTA GYVKVGERWF KPGCRQLCIC 1150
    EGNNRTRCVL WRCQAQEFCG QQDGIYGCHA QGSATCTVSG DPHYLTFDGA 1200
    LHHFTGTCTY TLTKPCWLRS LENSFLVSAT NEFRGGNLEA SYVRAVQVQV 1250
    FNLRISLIKG RKVTLDGRRV ALPLWPAQGR VSITSSGSFI LLYTDFGLQV 1300
    RYDGDHLVEV TVPSSYAGRL CGLCGNYNNN SLDDILQPDK RPASSSVRLG 1350
    ASWKINELSE PGCFAEGGKP PRCLGKEVAD AWRKNCDVLM NPQGPFSQCH 1400
    RVVAPQSSFS SCLYGQCATK GDTLTLCRSL QAYASLCARA GQALTWRNGT 1450
    FCPLKCPSGS SYSTCANPCP ATCLSLNNPS YCPSTLPCAE GCECQKGHIL 1500
    SGTSCVPLSQ CGCTTQRGSY HPVGESWYTD NSCSRLCTCS AHNNISCRQA 1550
    SCKPSQMCWP QDGLIRCRVA GMGVCRIPDT SHYVSFDGSY HAVRGNCTYV 1600
    LVKICHSTMD LPFFKISGEN GKREGQPPAF YLRQVYVDIF NTLVTLKQDQ 1650
    VLINGTRVSL PATTQIRGVR VISRDGYTVL TINIGVQVKF DGRGFLEVEI 1700
    PKAYYGRTCG VCGNFNDEEE DELMMPSDAL ALDDVMYVDS WRDKEIDPNC 1750
    QEDDRKTEAE SQEQPSANCR PADLERAQEQ CQAAFQAPAW ANCATRVVLS 1800
    PYVRSCTHKL CEFGGLNRAF CESLQAFGAA CQAQGIKPPV WRNSSFCPLD 1850
    CSAHSVYTSC VPSCLPSCQD PEGQCTGAGA PSTCEEGCIC EPGYVLSEQQ 1900
    CVARSQCGCR DARGTFLPVG RFRLSSGCSQ MCVCTAGAIE CRPFTCPSGS 1950
    QCEPNEDGKD FCQPNSSNLC SVFGDPHYRT FDGLSYRFQG RMTYTLVKTL 2000
    DVLPDGVEPL VVEGRNKVYP SLTPVFLQEI IVMVYGYTVQ LQAELELVVN 2050
    GQKVSIPYKP NEYLQVTLRG RRLYLVTDFE LVVSFNGRNN AVIAMPSTYL 2100
    GLVRGLCGNY DKNKRNDFML PNGSFTQNLL VFGNSWEVKA KEGHPRFSRA 2150
    IREEEEKNEE SGFQNVSECS PEQLELVNHT QACGVLVDPQ GPFAACHQIV 2200
    APGPFQEHCV FDLCAAPGPK EQEELRCQVL SGYAIICQES GPTLAGWRDH 2250
    THCALPCPAN TVYQSCMTPC PASCATLAVP RACDGPCVEG CASLPGYIYS 2300
    GAQSLPMAHC GCTNNGVYYQ QGDSFVTENC SQRCTCASSG VLLCEPLSCR 2350
    PGEICTLGNL TRGCFRDSPC LQNPCQNDGR CREQGTHFTC ECELGYGGDL 2400
    CTEPRGVPSP KKPEASNRVA ILLGMLMPTV LLVPAVTRVS RKRRRRRRPS 2450
    RERTQSQNRG KRAGTDCAPE QAYKVA 2476
    Length:2,476
    Mass (Da):270,365
    Last modified:November 1, 1996 - v1
    Checksum:iA13B690375A6548C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti823 – 8231C → V AA sequence (PubMed:7592795)Curated
    Sequence conflicti923 – 9231S → Y AA sequence (PubMed:7592795)Curated
    Sequence conflicti965 – 9651W → Y AA sequence (PubMed:7592795)Curated
    Sequence conflicti1241 – 12411S → K AA sequence (PubMed:7592795)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U40024 mRNA. Translation: AAC48486.1.
    PIRiT34022.
    RefSeqiNP_999548.1. NM_214383.1.
    UniGeneiSsc.82465.

    Genome annotation databases

    GeneIDi397676.
    KEGGissc:397676.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U40024 mRNA. Translation: AAC48486.1 .
    PIRi T34022.
    RefSeqi NP_999548.1. NM_214383.1.
    UniGenei Ssc.82465.

    3D structure databases

    ProteinModelPortali Q28983.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9823.ENSSSCP00000008195.

    Proteomic databases

    PRIDEi Q28983.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 397676.
    KEGGi ssc:397676.

    Organism-specific databases

    CTDi 7455.

    Phylogenomic databases

    eggNOGi NOG12793.
    HOGENOMi HOG000168448.
    HOVERGENi HBG000375.

    Family and domain databases

    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR000998. MAM_dom.
    IPR002919. TIL_dom.
    IPR025615. TILa_dom.
    IPR014853. Unchr_dom_Cys-rich.
    IPR001007. VWF_C.
    IPR001846. VWF_type-D.
    [Graphical view ]
    Pfami PF08742. C8. 4 hits.
    PF00008. EGF. 1 hit.
    PF00629. MAM. 2 hits.
    PF01826. TIL. 5 hits.
    PF12714. TILa. 5 hits.
    PF00094. VWD. 4 hits.
    [Graphical view ]
    SMARTi SM00832. C8. 4 hits.
    SM00181. EGF. 1 hit.
    SM00137. MAM. 1 hit.
    SM00214. VWC. 2 hits.
    SM00216. VWD. 4 hits.
    [Graphical view ]
    SUPFAMi SSF49899. SSF49899. 2 hits.
    SSF57567. SSF57567. 5 hits.
    PROSITEi PS00022. EGF_1. 1 hit.
    PS01186. EGF_2. 4 hits.
    PS50026. EGF_3. 1 hit.
    PS00740. MAM_1. 1 hit.
    PS50060. MAM_2. 2 hits.
    PS51233. VWFD. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A sperm membrane protein that binds in a species-specific manner to the egg extracellular matrix is homologous to von Willebrand factor."
      Hardy D.M., Garbers D.L.
      J. Biol. Chem. 270:26025-26028(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 823-830; 859-872; 883-890; 920-925; 960-967; 1235-1244; 1349-1354; 1518-1532; 1624-1656; 1658-1667; 1777-1795 AND 1914-1921.
      Strain: Meishan.
      Tissue: Testis.

    Entry informationi

    Entry nameiZAN_PIG
    AccessioniPrimary (citable) accession number: Q28983
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3