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Q28983 (ZAN_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Zonadhesin
Gene names
Name:ZAN
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus

Protein attributes

Sequence length2476 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds in a species-specific manner to the zona pellucida of the egg. May be involved in gamete recognition and/or signaling.

Subunit structure

Probably forms covalent oligomers.

Subcellular location

Cell membrane; Single-pass type I membrane protein. Note: Exclusively on the apical region of the sperm head By similarity.

Tissue specificity

In testis, primarily in haploid spermatids. Not in lung, liver, heart, spleen, brain, kidney, epididymis.

Domain

The MAM domains probably mediate sperm adhesion to the zona pellucida.

During sperm migration through the reproductive tracts, the mucin-like domain might inhibit inappropriate trapping of spermatozoa or promoting adhesion to the oviductal isthmus.

The VWFD domains 2 and 3 may mediate covalent oligomerization (By similarity to human intestinal mucin MUC2).

Post-translational modification

The MAM domains and the mucin-like domains are missing from the zonadhesin that binds to the egg extracellular matrix. Processing might occur during sperm maturation and/or capacitation.

Sequence similarities

Contains 1 EGF-like domain.

Contains 2 MAM domains.

Contains 5 TIL (trypsin inhibitory-like) domains.

Contains 5 VWFC domains.

Contains 4 VWFD domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Potential
Chain30 – 24762447Zonadhesin
PRO_0000007785

Regions

Topological domain30 – 24182389Extracellular Potential
Transmembrane2419 – 243921Helical; Potential
Topological domain2440 – 247637Cytoplasmic Potential
Domain31 – 144114MAM 1
Domain147 – 312166MAM 2
Domain690 – 73950TIL 1
Domain740 – 79455VWFC 1
Domain800 – 1015216VWFD 1
Domain1070 – 112354TIL 2
Domain1124 – 118057VWFC 2
Domain1185 – 1402218VWFD 2
Domain1456 – 151156TIL 3
Domain1512 – 156857VWFC 3
Domain1574 – 1784211VWFD 3
Domain1851 – 190757TIL 4
Domain1908 – 196356VWFC 4
Domain1969 – 2172204VWFD 4
Domain2257 – 231054TIL 5
Domain2311 – 236555VWFC 5
Domain2366 – 240237EGF-like
Region319 – 68736953 X approximate heptapeptide repeats (mucin-like domain)

Amino acid modifications

Glycosylation1091N-linked (GlcNAc...) Potential
Glycosylation2691N-linked (GlcNAc...) Potential
Glycosylation7351N-linked (GlcNAc...) Potential
Glycosylation7581N-linked (GlcNAc...) Potential
Glycosylation8331N-linked (GlcNAc...) Potential
Glycosylation11541N-linked (GlcNAc...) Potential
Glycosylation13291N-linked (GlcNAc...) Potential
Glycosylation14481N-linked (GlcNAc...) Potential
Glycosylation15441N-linked (GlcNAc...) Potential
Glycosylation15961N-linked (GlcNAc...) Potential
Glycosylation16541N-linked (GlcNAc...) Potential
Glycosylation18431N-linked (GlcNAc...) Potential
Glycosylation19651N-linked (GlcNAc...) Potential
Glycosylation21221N-linked (GlcNAc...) Potential
Glycosylation21651N-linked (GlcNAc...) Potential
Glycosylation21781N-linked (GlcNAc...) Potential
Glycosylation23291N-linked (GlcNAc...) Potential
Glycosylation23591N-linked (GlcNAc...) Potential
Disulfide bond2370 ↔ 2381 By similarity
Disulfide bond2375 ↔ 2390 By similarity
Disulfide bond2392 ↔ 2401 By similarity

Experimental info

Sequence conflict8231C → V AA sequence Ref.1
Sequence conflict9231S → Y AA sequence Ref.1
Sequence conflict9651W → Y AA sequence Ref.1
Sequence conflict12411S → K AA sequence Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q28983 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: A13B690375A6548C

FASTA2,476270,365
        10         20         30         40         50         60 
MLGLPALAGP MAMPHPPLIP STPTLLAFSF PGGFYMLLDP KNAKPRQRSA LLSPLIQSSG 

        70         80         90        100        110        120 
CLSLSFQYTQ RGQASGATLM VYASVLGSIR KHTLFSGQPG PSWQPVSVNY TSQGQIQFTL 

       130        140        150        160        170        180 
VGVFGKIPEP AVAVDAISIA PCEESFPQCD FEDNAHPFCD WVQASQDGGY WRQGNKNTFI 

       190        200        210        220        230        240 
QPAGPFGISL NGEGHYIFLE TDKFSQAGQS FRLVSRPFCA PAVICVTFTY HMYGLGQGTK 

       250        260        270        280        290        300 
LRLLLGSPAG SPPSSLWERV GPQSPEWLNT SVTIPSGHQQ PMQLIFEAVR GTNTAFVVAL 

       310        320        330        340        350        360 
GFVLINHGTC RGPSETSVST EKPVAPTEKP TVPSEIYTIP TEKPMVHMEK PIVHTEKPTV 

       370        380        390        400        410        420 
PTEKPTIPTE KSTVPTKKPT VFKEPTLPPE GPTVPAERPT TPPEGPAVPP KGPTVLTEWP 

       430        440        450        460        470        480 
TSHTEKSTVH TEKPILPTGK STIPTEKPMV PTKRTTTPTE RTTIPAEKPT VPIEKPMVPT 

       490        500        510        520        530        540 
ERTTIPTERT TIPTEKPTVP TEKLTVPTEK PIVPTEKPIV PTEKHTIPTE KLTVLTERTT 

       550        560        570        580        590        600 
TPTERTTIPT EKPTVPTEKP SVPTEKPTVP TEEPTIPTEK LTVPTERTTT PTKRTTTPTI 

       610        620        630        640        650        660 
RTTTPTIRTT TPTERTTTPT IRTTTPTERT TIPTKKTTVP TEKTIIPTER TIAPTTPQPS 

       670        680        690        700        710        720 
PTLVPTQPAA VVMPSTSATT VTPRTTIASC PPNAHFERCA CPVSCQSPTP NCELFCKPGC 

       730        740        750        760        770        780 
VCDPGFLFSG SHCVNASSCD CFYNDNYYKL GTDWFSPNCT EHCHCRPSSR MECQTFKCGT 

       790        800        810        820        830        840 
HTVCQLKNGQ YGCHPYGSAT CSVYGDPHYL TFDGRRFNFM GKCTYILAQP CGNLTEHFFR 

       850        860        870        880        890        900 
VLVKKEERGQ EGVSCLSKVY VTLPESTVTL LKGRHTLVGG QRVTLPAIPS RGVFLAPSGR 

       910        920        930        940        950        960 
FVELQTAFGL RVRWDGDQQL FVSVPSTFSG KLCGLCGDYD GDSSNDNQKP DGSPAKDEKE 

       970        980        990       1000       1010       1020 
LGSSWQTSED ADQQCEENQV SPPSCNTALQ NTMSGPEFCG QLVAPHGVFE ACLPHLRASS 

      1030       1040       1050       1060       1070       1080 
FFKSCTFDMC NFQGLQHMLC AHMSALTENC QDAGYTVKPW RGPQFCPLAC PRNSRYTLCA 

      1090       1100       1110       1120       1130       1140 
RLCPDTCHSE FSGRACKDRC VEGCECDPGF VLSGLQCVSR SECGCLDSTA GYVKVGERWF 

      1150       1160       1170       1180       1190       1200 
KPGCRQLCIC EGNNRTRCVL WRCQAQEFCG QQDGIYGCHA QGSATCTVSG DPHYLTFDGA 

      1210       1220       1230       1240       1250       1260 
LHHFTGTCTY TLTKPCWLRS LENSFLVSAT NEFRGGNLEA SYVRAVQVQV FNLRISLIKG 

      1270       1280       1290       1300       1310       1320 
RKVTLDGRRV ALPLWPAQGR VSITSSGSFI LLYTDFGLQV RYDGDHLVEV TVPSSYAGRL 

      1330       1340       1350       1360       1370       1380 
CGLCGNYNNN SLDDILQPDK RPASSSVRLG ASWKINELSE PGCFAEGGKP PRCLGKEVAD 

      1390       1400       1410       1420       1430       1440 
AWRKNCDVLM NPQGPFSQCH RVVAPQSSFS SCLYGQCATK GDTLTLCRSL QAYASLCARA 

      1450       1460       1470       1480       1490       1500 
GQALTWRNGT FCPLKCPSGS SYSTCANPCP ATCLSLNNPS YCPSTLPCAE GCECQKGHIL 

      1510       1520       1530       1540       1550       1560 
SGTSCVPLSQ CGCTTQRGSY HPVGESWYTD NSCSRLCTCS AHNNISCRQA SCKPSQMCWP 

      1570       1580       1590       1600       1610       1620 
QDGLIRCRVA GMGVCRIPDT SHYVSFDGSY HAVRGNCTYV LVKICHSTMD LPFFKISGEN 

      1630       1640       1650       1660       1670       1680 
GKREGQPPAF YLRQVYVDIF NTLVTLKQDQ VLINGTRVSL PATTQIRGVR VISRDGYTVL 

      1690       1700       1710       1720       1730       1740 
TINIGVQVKF DGRGFLEVEI PKAYYGRTCG VCGNFNDEEE DELMMPSDAL ALDDVMYVDS 

      1750       1760       1770       1780       1790       1800 
WRDKEIDPNC QEDDRKTEAE SQEQPSANCR PADLERAQEQ CQAAFQAPAW ANCATRVVLS 

      1810       1820       1830       1840       1850       1860 
PYVRSCTHKL CEFGGLNRAF CESLQAFGAA CQAQGIKPPV WRNSSFCPLD CSAHSVYTSC 

      1870       1880       1890       1900       1910       1920 
VPSCLPSCQD PEGQCTGAGA PSTCEEGCIC EPGYVLSEQQ CVARSQCGCR DARGTFLPVG 

      1930       1940       1950       1960       1970       1980 
RFRLSSGCSQ MCVCTAGAIE CRPFTCPSGS QCEPNEDGKD FCQPNSSNLC SVFGDPHYRT 

      1990       2000       2010       2020       2030       2040 
FDGLSYRFQG RMTYTLVKTL DVLPDGVEPL VVEGRNKVYP SLTPVFLQEI IVMVYGYTVQ 

      2050       2060       2070       2080       2090       2100 
LQAELELVVN GQKVSIPYKP NEYLQVTLRG RRLYLVTDFE LVVSFNGRNN AVIAMPSTYL 

      2110       2120       2130       2140       2150       2160 
GLVRGLCGNY DKNKRNDFML PNGSFTQNLL VFGNSWEVKA KEGHPRFSRA IREEEEKNEE 

      2170       2180       2190       2200       2210       2220 
SGFQNVSECS PEQLELVNHT QACGVLVDPQ GPFAACHQIV APGPFQEHCV FDLCAAPGPK 

      2230       2240       2250       2260       2270       2280 
EQEELRCQVL SGYAIICQES GPTLAGWRDH THCALPCPAN TVYQSCMTPC PASCATLAVP 

      2290       2300       2310       2320       2330       2340 
RACDGPCVEG CASLPGYIYS GAQSLPMAHC GCTNNGVYYQ QGDSFVTENC SQRCTCASSG 

      2350       2360       2370       2380       2390       2400 
VLLCEPLSCR PGEICTLGNL TRGCFRDSPC LQNPCQNDGR CREQGTHFTC ECELGYGGDL 

      2410       2420       2430       2440       2450       2460 
CTEPRGVPSP KKPEASNRVA ILLGMLMPTV LLVPAVTRVS RKRRRRRRPS RERTQSQNRG 

      2470 
KRAGTDCAPE QAYKVA 

« Hide

References

[1]"A sperm membrane protein that binds in a species-specific manner to the egg extracellular matrix is homologous to von Willebrand factor."
Hardy D.M., Garbers D.L.
J. Biol. Chem. 270:26025-26028(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 823-830; 859-872; 883-890; 920-925; 960-967; 1235-1244; 1349-1354; 1518-1532; 1624-1656; 1658-1667; 1777-1795 AND 1914-1921.
Strain: Meishan.
Tissue: Testis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U40024 mRNA. Translation: AAC48486.1.
PIRT34022.
RefSeqNP_999548.1. NM_214383.1.
UniGeneSsc.82465.

3D structure databases

ProteinModelPortalQ28983.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9823.ENSSSCP00000008195.

Proteomic databases

PRIDEQ28983.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID397676.
KEGGssc:397676.

Organism-specific databases

CTD7455.

Phylogenomic databases

eggNOGNOG12793.
HOGENOMHOG000168448.
HOVERGENHBG000375.

Family and domain databases

InterProIPR008985. ConA-like_lec_gl_sf.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000998. MAM_dom.
IPR002919. TIL_dom.
IPR025615. TILa_dom.
IPR014853. Unchr_dom_Cys-rich.
IPR001007. VWF_C.
IPR001846. VWF_type-D.
[Graphical view]
PfamPF08742. C8. 4 hits.
PF00008. EGF. 1 hit.
PF00629. MAM. 2 hits.
PF01826. TIL. 5 hits.
PF12714. TILa. 5 hits.
PF00094. VWD. 4 hits.
[Graphical view]
SMARTSM00832. C8. 4 hits.
SM00181. EGF. 1 hit.
SM00137. MAM. 1 hit.
SM00214. VWC. 2 hits.
SM00216. VWD. 4 hits.
[Graphical view]
SUPFAMSSF49899. SSF49899. 2 hits.
SSF57567. SSF57567. 5 hits.
PROSITEPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 4 hits.
PS50026. EGF_3. 1 hit.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 2 hits.
PS51233. VWFD. 4 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameZAN_PIG
AccessionPrimary (citable) accession number: Q28983
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families