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Q28983

- ZAN_PIG

UniProt

Q28983 - ZAN_PIG

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Protein
Zonadhesin
Gene
ZAN
Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Binds in a species-specific manner to the zona pellucida of the egg. May be involved in gamete recognition and/or signaling.

GO - Molecular functioni

  1. extracellular matrix binding Source: MGI
Complete GO annotation...

GO - Biological processi

  1. binding of sperm to zona pellucida Source: MGI
  2. cell adhesion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Zonadhesin
Gene namesi
Name:ZAN
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Unplaced

Subcellular locationi

Cell membrane; Single-pass type I membrane protein
Note: Exclusively on the apical region of the sperm head By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini30 – 24182389Extracellular Reviewed prediction
Add
BLAST
Transmembranei2419 – 243921Helical; Reviewed prediction
Add
BLAST
Topological domaini2440 – 247637Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929 Reviewed prediction
Add
BLAST
Chaini30 – 24762447Zonadhesin
PRO_0000007785Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi109 – 1091N-linked (GlcNAc...) Reviewed prediction
Glycosylationi269 – 2691N-linked (GlcNAc...) Reviewed prediction
Glycosylationi735 – 7351N-linked (GlcNAc...) Reviewed prediction
Glycosylationi758 – 7581N-linked (GlcNAc...) Reviewed prediction
Glycosylationi833 – 8331N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1154 – 11541N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1329 – 13291N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1448 – 14481N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1544 – 15441N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1596 – 15961N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1654 – 16541N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1843 – 18431N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1965 – 19651N-linked (GlcNAc...) Reviewed prediction
Glycosylationi2122 – 21221N-linked (GlcNAc...) Reviewed prediction
Glycosylationi2165 – 21651N-linked (GlcNAc...) Reviewed prediction
Glycosylationi2178 – 21781N-linked (GlcNAc...) Reviewed prediction
Glycosylationi2329 – 23291N-linked (GlcNAc...) Reviewed prediction
Glycosylationi2359 – 23591N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi2370 ↔ 2381 By similarity
Disulfide bondi2375 ↔ 2390 By similarity
Disulfide bondi2392 ↔ 2401 By similarity

Post-translational modificationi

The MAM domains and the mucin-like domains are missing from the zonadhesin that binds to the egg extracellular matrix. Processing might occur during sperm maturation and/or capacitation.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ28983.

Expressioni

Tissue specificityi

In testis, primarily in haploid spermatids. Not in lung, liver, heart, spleen, brain, kidney, epididymis.

Interactioni

Subunit structurei

Probably forms covalent oligomers.

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000008195.

Structurei

3D structure databases

ProteinModelPortaliQ28983.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 144114MAM 1
Add
BLAST
Domaini147 – 312166MAM 2
Add
BLAST
Domaini690 – 73950TIL 1
Add
BLAST
Domaini740 – 79455VWFC 1
Add
BLAST
Domaini800 – 1015216VWFD 1
Add
BLAST
Domaini1070 – 112354TIL 2
Add
BLAST
Domaini1124 – 118057VWFC 2
Add
BLAST
Domaini1185 – 1402218VWFD 2
Add
BLAST
Domaini1456 – 151156TIL 3
Add
BLAST
Domaini1512 – 156857VWFC 3
Add
BLAST
Domaini1574 – 1784211VWFD 3
Add
BLAST
Domaini1851 – 190757TIL 4
Add
BLAST
Domaini1908 – 196356VWFC 4
Add
BLAST
Domaini1969 – 2172204VWFD 4
Add
BLAST
Domaini2257 – 231054TIL 5
Add
BLAST
Domaini2311 – 236555VWFC 5
Add
BLAST
Domaini2366 – 240237EGF-like
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni319 – 68736953 X approximate heptapeptide repeats (mucin-like domain)
Add
BLAST

Domaini

The MAM domains probably mediate sperm adhesion to the zona pellucida.
During sperm migration through the reproductive tracts, the mucin-like domain might inhibit inappropriate trapping of spermatozoa or promoting adhesion to the oviductal isthmus.
The VWFD domains 2 and 3 may mediate covalent oligomerization (By similarity to human intestinal mucin MUC2).

Sequence similaritiesi

Contains 1 EGF-like domain.
Contains 2 MAM domains.
Contains 5 VWFC domains.
Contains 4 VWFD domains.

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG12793.
HOGENOMiHOG000168448.
HOVERGENiHBG000375.

Family and domain databases

InterProiIPR008985. ConA-like_lec_gl_sf.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000998. MAM_dom.
IPR002919. TIL_dom.
IPR025615. TILa_dom.
IPR014853. Unchr_dom_Cys-rich.
IPR001007. VWF_C.
IPR001846. VWF_type-D.
[Graphical view]
PfamiPF08742. C8. 4 hits.
PF00008. EGF. 1 hit.
PF00629. MAM. 2 hits.
PF01826. TIL. 5 hits.
PF12714. TILa. 5 hits.
PF00094. VWD. 4 hits.
[Graphical view]
SMARTiSM00832. C8. 4 hits.
SM00181. EGF. 1 hit.
SM00137. MAM. 1 hit.
SM00214. VWC. 2 hits.
SM00216. VWD. 4 hits.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.
SSF57567. SSF57567. 5 hits.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 4 hits.
PS50026. EGF_3. 1 hit.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 2 hits.
PS51233. VWFD. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q28983-1 [UniParc]FASTAAdd to Basket

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MLGLPALAGP MAMPHPPLIP STPTLLAFSF PGGFYMLLDP KNAKPRQRSA     50
LLSPLIQSSG CLSLSFQYTQ RGQASGATLM VYASVLGSIR KHTLFSGQPG 100
PSWQPVSVNY TSQGQIQFTL VGVFGKIPEP AVAVDAISIA PCEESFPQCD 150
FEDNAHPFCD WVQASQDGGY WRQGNKNTFI QPAGPFGISL NGEGHYIFLE 200
TDKFSQAGQS FRLVSRPFCA PAVICVTFTY HMYGLGQGTK LRLLLGSPAG 250
SPPSSLWERV GPQSPEWLNT SVTIPSGHQQ PMQLIFEAVR GTNTAFVVAL 300
GFVLINHGTC RGPSETSVST EKPVAPTEKP TVPSEIYTIP TEKPMVHMEK 350
PIVHTEKPTV PTEKPTIPTE KSTVPTKKPT VFKEPTLPPE GPTVPAERPT 400
TPPEGPAVPP KGPTVLTEWP TSHTEKSTVH TEKPILPTGK STIPTEKPMV 450
PTKRTTTPTE RTTIPAEKPT VPIEKPMVPT ERTTIPTERT TIPTEKPTVP 500
TEKLTVPTEK PIVPTEKPIV PTEKHTIPTE KLTVLTERTT TPTERTTIPT 550
EKPTVPTEKP SVPTEKPTVP TEEPTIPTEK LTVPTERTTT PTKRTTTPTI 600
RTTTPTIRTT TPTERTTTPT IRTTTPTERT TIPTKKTTVP TEKTIIPTER 650
TIAPTTPQPS PTLVPTQPAA VVMPSTSATT VTPRTTIASC PPNAHFERCA 700
CPVSCQSPTP NCELFCKPGC VCDPGFLFSG SHCVNASSCD CFYNDNYYKL 750
GTDWFSPNCT EHCHCRPSSR MECQTFKCGT HTVCQLKNGQ YGCHPYGSAT 800
CSVYGDPHYL TFDGRRFNFM GKCTYILAQP CGNLTEHFFR VLVKKEERGQ 850
EGVSCLSKVY VTLPESTVTL LKGRHTLVGG QRVTLPAIPS RGVFLAPSGR 900
FVELQTAFGL RVRWDGDQQL FVSVPSTFSG KLCGLCGDYD GDSSNDNQKP 950
DGSPAKDEKE LGSSWQTSED ADQQCEENQV SPPSCNTALQ NTMSGPEFCG 1000
QLVAPHGVFE ACLPHLRASS FFKSCTFDMC NFQGLQHMLC AHMSALTENC 1050
QDAGYTVKPW RGPQFCPLAC PRNSRYTLCA RLCPDTCHSE FSGRACKDRC 1100
VEGCECDPGF VLSGLQCVSR SECGCLDSTA GYVKVGERWF KPGCRQLCIC 1150
EGNNRTRCVL WRCQAQEFCG QQDGIYGCHA QGSATCTVSG DPHYLTFDGA 1200
LHHFTGTCTY TLTKPCWLRS LENSFLVSAT NEFRGGNLEA SYVRAVQVQV 1250
FNLRISLIKG RKVTLDGRRV ALPLWPAQGR VSITSSGSFI LLYTDFGLQV 1300
RYDGDHLVEV TVPSSYAGRL CGLCGNYNNN SLDDILQPDK RPASSSVRLG 1350
ASWKINELSE PGCFAEGGKP PRCLGKEVAD AWRKNCDVLM NPQGPFSQCH 1400
RVVAPQSSFS SCLYGQCATK GDTLTLCRSL QAYASLCARA GQALTWRNGT 1450
FCPLKCPSGS SYSTCANPCP ATCLSLNNPS YCPSTLPCAE GCECQKGHIL 1500
SGTSCVPLSQ CGCTTQRGSY HPVGESWYTD NSCSRLCTCS AHNNISCRQA 1550
SCKPSQMCWP QDGLIRCRVA GMGVCRIPDT SHYVSFDGSY HAVRGNCTYV 1600
LVKICHSTMD LPFFKISGEN GKREGQPPAF YLRQVYVDIF NTLVTLKQDQ 1650
VLINGTRVSL PATTQIRGVR VISRDGYTVL TINIGVQVKF DGRGFLEVEI 1700
PKAYYGRTCG VCGNFNDEEE DELMMPSDAL ALDDVMYVDS WRDKEIDPNC 1750
QEDDRKTEAE SQEQPSANCR PADLERAQEQ CQAAFQAPAW ANCATRVVLS 1800
PYVRSCTHKL CEFGGLNRAF CESLQAFGAA CQAQGIKPPV WRNSSFCPLD 1850
CSAHSVYTSC VPSCLPSCQD PEGQCTGAGA PSTCEEGCIC EPGYVLSEQQ 1900
CVARSQCGCR DARGTFLPVG RFRLSSGCSQ MCVCTAGAIE CRPFTCPSGS 1950
QCEPNEDGKD FCQPNSSNLC SVFGDPHYRT FDGLSYRFQG RMTYTLVKTL 2000
DVLPDGVEPL VVEGRNKVYP SLTPVFLQEI IVMVYGYTVQ LQAELELVVN 2050
GQKVSIPYKP NEYLQVTLRG RRLYLVTDFE LVVSFNGRNN AVIAMPSTYL 2100
GLVRGLCGNY DKNKRNDFML PNGSFTQNLL VFGNSWEVKA KEGHPRFSRA 2150
IREEEEKNEE SGFQNVSECS PEQLELVNHT QACGVLVDPQ GPFAACHQIV 2200
APGPFQEHCV FDLCAAPGPK EQEELRCQVL SGYAIICQES GPTLAGWRDH 2250
THCALPCPAN TVYQSCMTPC PASCATLAVP RACDGPCVEG CASLPGYIYS 2300
GAQSLPMAHC GCTNNGVYYQ QGDSFVTENC SQRCTCASSG VLLCEPLSCR 2350
PGEICTLGNL TRGCFRDSPC LQNPCQNDGR CREQGTHFTC ECELGYGGDL 2400
CTEPRGVPSP KKPEASNRVA ILLGMLMPTV LLVPAVTRVS RKRRRRRRPS 2450
RERTQSQNRG KRAGTDCAPE QAYKVA 2476
Length:2,476
Mass (Da):270,365
Last modified:November 1, 1996 - v1
Checksum:iA13B690375A6548C
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti823 – 8231C → V AA sequence 1 Publication
Sequence conflicti923 – 9231S → Y AA sequence 1 Publication
Sequence conflicti965 – 9651W → Y AA sequence 1 Publication
Sequence conflicti1241 – 12411S → K AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U40024 mRNA. Translation: AAC48486.1.
PIRiT34022.
RefSeqiNP_999548.1. NM_214383.1.
UniGeneiSsc.82465.

Genome annotation databases

GeneIDi397676.
KEGGissc:397676.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U40024 mRNA. Translation: AAC48486.1 .
PIRi T34022.
RefSeqi NP_999548.1. NM_214383.1.
UniGenei Ssc.82465.

3D structure databases

ProteinModelPortali Q28983.
ModBasei Search...

Protein-protein interaction databases

STRINGi 9823.ENSSSCP00000008195.

Proteomic databases

PRIDEi Q28983.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 397676.
KEGGi ssc:397676.

Organism-specific databases

CTDi 7455.

Phylogenomic databases

eggNOGi NOG12793.
HOGENOMi HOG000168448.
HOVERGENi HBG000375.

Family and domain databases

InterProi IPR008985. ConA-like_lec_gl_sf.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000998. MAM_dom.
IPR002919. TIL_dom.
IPR025615. TILa_dom.
IPR014853. Unchr_dom_Cys-rich.
IPR001007. VWF_C.
IPR001846. VWF_type-D.
[Graphical view ]
Pfami PF08742. C8. 4 hits.
PF00008. EGF. 1 hit.
PF00629. MAM. 2 hits.
PF01826. TIL. 5 hits.
PF12714. TILa. 5 hits.
PF00094. VWD. 4 hits.
[Graphical view ]
SMARTi SM00832. C8. 4 hits.
SM00181. EGF. 1 hit.
SM00137. MAM. 1 hit.
SM00214. VWC. 2 hits.
SM00216. VWD. 4 hits.
[Graphical view ]
SUPFAMi SSF49899. SSF49899. 2 hits.
SSF57567. SSF57567. 5 hits.
PROSITEi PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 4 hits.
PS50026. EGF_3. 1 hit.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 2 hits.
PS51233. VWFD. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A sperm membrane protein that binds in a species-specific manner to the egg extracellular matrix is homologous to von Willebrand factor."
    Hardy D.M., Garbers D.L.
    J. Biol. Chem. 270:26025-26028(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 823-830; 859-872; 883-890; 920-925; 960-967; 1235-1244; 1349-1354; 1518-1532; 1624-1656; 1658-1667; 1777-1795 AND 1914-1921.
    Strain: Meishan.
    Tissue: Testis.

Entry informationi

Entry nameiZAN_PIG
AccessioniPrimary (citable) accession number: Q28983
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi