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Q28983

- ZAN_PIG

UniProt

Q28983 - ZAN_PIG

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Protein

Zonadhesin

Gene

ZAN

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Binds in a species-specific manner to the zona pellucida of the egg. May be involved in gamete recognition and/or signaling.

GO - Molecular functioni

  1. extracellular matrix binding Source: MGI

GO - Biological processi

  1. binding of sperm to zona pellucida Source: MGI
  2. cell adhesion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Zonadhesin
Gene namesi
Name:ZAN
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Unplaced

Subcellular locationi

Cell membrane; Single-pass type I membrane protein
Note: Exclusively on the apical region of the sperm head.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini30 – 24182389ExtracellularSequence AnalysisAdd
BLAST
Transmembranei2419 – 243921HelicalSequence AnalysisAdd
BLAST
Topological domaini2440 – 247637CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence AnalysisAdd
BLAST
Chaini30 – 24762447ZonadhesinPRO_0000007785Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi109 – 1091N-linked (GlcNAc...)Sequence Analysis
Glycosylationi269 – 2691N-linked (GlcNAc...)Sequence Analysis
Glycosylationi735 – 7351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi758 – 7581N-linked (GlcNAc...)Sequence Analysis
Glycosylationi833 – 8331N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1154 – 11541N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1329 – 13291N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1448 – 14481N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1544 – 15441N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1596 – 15961N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1654 – 16541N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1843 – 18431N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1965 – 19651N-linked (GlcNAc...)Sequence Analysis
Glycosylationi2122 – 21221N-linked (GlcNAc...)Sequence Analysis
Glycosylationi2165 – 21651N-linked (GlcNAc...)Sequence Analysis
Glycosylationi2178 – 21781N-linked (GlcNAc...)Sequence Analysis
Glycosylationi2329 – 23291N-linked (GlcNAc...)Sequence Analysis
Glycosylationi2359 – 23591N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi2370 ↔ 2381By similarity
Disulfide bondi2375 ↔ 2390By similarity
Disulfide bondi2392 ↔ 2401By similarity

Post-translational modificationi

The MAM domains and the mucin-like domains are missing from the zonadhesin that binds to the egg extracellular matrix. Processing might occur during sperm maturation and/or capacitation.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ28983.

Expressioni

Tissue specificityi

In testis, primarily in haploid spermatids. Not in lung, liver, heart, spleen, brain, kidney, epididymis.

Interactioni

Subunit structurei

Probably forms covalent oligomers.

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000008195.

Structurei

3D structure databases

ProteinModelPortaliQ28983.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 144114MAM 1PROSITE-ProRule annotationAdd
BLAST
Domaini147 – 312166MAM 2PROSITE-ProRule annotationAdd
BLAST
Domaini690 – 73950TIL 1Add
BLAST
Domaini740 – 79455VWFC 1Add
BLAST
Domaini800 – 1015216VWFD 1PROSITE-ProRule annotationAdd
BLAST
Domaini1070 – 112354TIL 2Add
BLAST
Domaini1124 – 118057VWFC 2Add
BLAST
Domaini1185 – 1402218VWFD 2PROSITE-ProRule annotationAdd
BLAST
Domaini1456 – 151156TIL 3Add
BLAST
Domaini1512 – 156857VWFC 3Add
BLAST
Domaini1574 – 1784211VWFD 3PROSITE-ProRule annotationAdd
BLAST
Domaini1851 – 190757TIL 4Add
BLAST
Domaini1908 – 196356VWFC 4Add
BLAST
Domaini1969 – 2172204VWFD 4PROSITE-ProRule annotationAdd
BLAST
Domaini2257 – 231054TIL 5Add
BLAST
Domaini2311 – 236555VWFC 5Add
BLAST
Domaini2366 – 240237EGF-likePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni319 – 68736953 X approximate heptapeptide repeats (mucin-like domain)Add
BLAST

Domaini

The MAM domains probably mediate sperm adhesion to the zona pellucida.
During sperm migration through the reproductive tracts, the mucin-like domain might inhibit inappropriate trapping of spermatozoa or promoting adhesion to the oviductal isthmus.
The VWFD domains 2 and 3 may mediate covalent oligomerization (By similarity to human intestinal mucin MUC2).

Sequence similaritiesi

Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains 2 MAM domains.PROSITE-ProRule annotation
Contains 5 VWFC domains.Curated
Contains 4 VWFD domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG12793.
HOGENOMiHOG000168448.
HOVERGENiHBG000375.
InParanoidiQ28983.

Family and domain databases

InterProiIPR013320. ConA-like_dom.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000998. MAM_dom.
IPR002919. TIL_dom.
IPR025615. TILa_dom.
IPR014853. Unchr_dom_Cys-rich.
IPR001007. VWF_C.
IPR001846. VWF_type-D.
[Graphical view]
PfamiPF08742. C8. 4 hits.
PF00008. EGF. 1 hit.
PF00629. MAM. 2 hits.
PF01826. TIL. 5 hits.
PF12714. TILa. 5 hits.
PF00094. VWD. 4 hits.
[Graphical view]
SMARTiSM00832. C8. 4 hits.
SM00181. EGF. 1 hit.
SM00137. MAM. 1 hit.
SM00214. VWC. 2 hits.
SM00216. VWD. 4 hits.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.
SSF57567. SSF57567. 5 hits.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 4 hits.
PS50026. EGF_3. 1 hit.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 2 hits.
PS51233. VWFD. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q28983-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MLGLPALAGP MAMPHPPLIP STPTLLAFSF PGGFYMLLDP KNAKPRQRSA
60 70 80 90 100
LLSPLIQSSG CLSLSFQYTQ RGQASGATLM VYASVLGSIR KHTLFSGQPG
110 120 130 140 150
PSWQPVSVNY TSQGQIQFTL VGVFGKIPEP AVAVDAISIA PCEESFPQCD
160 170 180 190 200
FEDNAHPFCD WVQASQDGGY WRQGNKNTFI QPAGPFGISL NGEGHYIFLE
210 220 230 240 250
TDKFSQAGQS FRLVSRPFCA PAVICVTFTY HMYGLGQGTK LRLLLGSPAG
260 270 280 290 300
SPPSSLWERV GPQSPEWLNT SVTIPSGHQQ PMQLIFEAVR GTNTAFVVAL
310 320 330 340 350
GFVLINHGTC RGPSETSVST EKPVAPTEKP TVPSEIYTIP TEKPMVHMEK
360 370 380 390 400
PIVHTEKPTV PTEKPTIPTE KSTVPTKKPT VFKEPTLPPE GPTVPAERPT
410 420 430 440 450
TPPEGPAVPP KGPTVLTEWP TSHTEKSTVH TEKPILPTGK STIPTEKPMV
460 470 480 490 500
PTKRTTTPTE RTTIPAEKPT VPIEKPMVPT ERTTIPTERT TIPTEKPTVP
510 520 530 540 550
TEKLTVPTEK PIVPTEKPIV PTEKHTIPTE KLTVLTERTT TPTERTTIPT
560 570 580 590 600
EKPTVPTEKP SVPTEKPTVP TEEPTIPTEK LTVPTERTTT PTKRTTTPTI
610 620 630 640 650
RTTTPTIRTT TPTERTTTPT IRTTTPTERT TIPTKKTTVP TEKTIIPTER
660 670 680 690 700
TIAPTTPQPS PTLVPTQPAA VVMPSTSATT VTPRTTIASC PPNAHFERCA
710 720 730 740 750
CPVSCQSPTP NCELFCKPGC VCDPGFLFSG SHCVNASSCD CFYNDNYYKL
760 770 780 790 800
GTDWFSPNCT EHCHCRPSSR MECQTFKCGT HTVCQLKNGQ YGCHPYGSAT
810 820 830 840 850
CSVYGDPHYL TFDGRRFNFM GKCTYILAQP CGNLTEHFFR VLVKKEERGQ
860 870 880 890 900
EGVSCLSKVY VTLPESTVTL LKGRHTLVGG QRVTLPAIPS RGVFLAPSGR
910 920 930 940 950
FVELQTAFGL RVRWDGDQQL FVSVPSTFSG KLCGLCGDYD GDSSNDNQKP
960 970 980 990 1000
DGSPAKDEKE LGSSWQTSED ADQQCEENQV SPPSCNTALQ NTMSGPEFCG
1010 1020 1030 1040 1050
QLVAPHGVFE ACLPHLRASS FFKSCTFDMC NFQGLQHMLC AHMSALTENC
1060 1070 1080 1090 1100
QDAGYTVKPW RGPQFCPLAC PRNSRYTLCA RLCPDTCHSE FSGRACKDRC
1110 1120 1130 1140 1150
VEGCECDPGF VLSGLQCVSR SECGCLDSTA GYVKVGERWF KPGCRQLCIC
1160 1170 1180 1190 1200
EGNNRTRCVL WRCQAQEFCG QQDGIYGCHA QGSATCTVSG DPHYLTFDGA
1210 1220 1230 1240 1250
LHHFTGTCTY TLTKPCWLRS LENSFLVSAT NEFRGGNLEA SYVRAVQVQV
1260 1270 1280 1290 1300
FNLRISLIKG RKVTLDGRRV ALPLWPAQGR VSITSSGSFI LLYTDFGLQV
1310 1320 1330 1340 1350
RYDGDHLVEV TVPSSYAGRL CGLCGNYNNN SLDDILQPDK RPASSSVRLG
1360 1370 1380 1390 1400
ASWKINELSE PGCFAEGGKP PRCLGKEVAD AWRKNCDVLM NPQGPFSQCH
1410 1420 1430 1440 1450
RVVAPQSSFS SCLYGQCATK GDTLTLCRSL QAYASLCARA GQALTWRNGT
1460 1470 1480 1490 1500
FCPLKCPSGS SYSTCANPCP ATCLSLNNPS YCPSTLPCAE GCECQKGHIL
1510 1520 1530 1540 1550
SGTSCVPLSQ CGCTTQRGSY HPVGESWYTD NSCSRLCTCS AHNNISCRQA
1560 1570 1580 1590 1600
SCKPSQMCWP QDGLIRCRVA GMGVCRIPDT SHYVSFDGSY HAVRGNCTYV
1610 1620 1630 1640 1650
LVKICHSTMD LPFFKISGEN GKREGQPPAF YLRQVYVDIF NTLVTLKQDQ
1660 1670 1680 1690 1700
VLINGTRVSL PATTQIRGVR VISRDGYTVL TINIGVQVKF DGRGFLEVEI
1710 1720 1730 1740 1750
PKAYYGRTCG VCGNFNDEEE DELMMPSDAL ALDDVMYVDS WRDKEIDPNC
1760 1770 1780 1790 1800
QEDDRKTEAE SQEQPSANCR PADLERAQEQ CQAAFQAPAW ANCATRVVLS
1810 1820 1830 1840 1850
PYVRSCTHKL CEFGGLNRAF CESLQAFGAA CQAQGIKPPV WRNSSFCPLD
1860 1870 1880 1890 1900
CSAHSVYTSC VPSCLPSCQD PEGQCTGAGA PSTCEEGCIC EPGYVLSEQQ
1910 1920 1930 1940 1950
CVARSQCGCR DARGTFLPVG RFRLSSGCSQ MCVCTAGAIE CRPFTCPSGS
1960 1970 1980 1990 2000
QCEPNEDGKD FCQPNSSNLC SVFGDPHYRT FDGLSYRFQG RMTYTLVKTL
2010 2020 2030 2040 2050
DVLPDGVEPL VVEGRNKVYP SLTPVFLQEI IVMVYGYTVQ LQAELELVVN
2060 2070 2080 2090 2100
GQKVSIPYKP NEYLQVTLRG RRLYLVTDFE LVVSFNGRNN AVIAMPSTYL
2110 2120 2130 2140 2150
GLVRGLCGNY DKNKRNDFML PNGSFTQNLL VFGNSWEVKA KEGHPRFSRA
2160 2170 2180 2190 2200
IREEEEKNEE SGFQNVSECS PEQLELVNHT QACGVLVDPQ GPFAACHQIV
2210 2220 2230 2240 2250
APGPFQEHCV FDLCAAPGPK EQEELRCQVL SGYAIICQES GPTLAGWRDH
2260 2270 2280 2290 2300
THCALPCPAN TVYQSCMTPC PASCATLAVP RACDGPCVEG CASLPGYIYS
2310 2320 2330 2340 2350
GAQSLPMAHC GCTNNGVYYQ QGDSFVTENC SQRCTCASSG VLLCEPLSCR
2360 2370 2380 2390 2400
PGEICTLGNL TRGCFRDSPC LQNPCQNDGR CREQGTHFTC ECELGYGGDL
2410 2420 2430 2440 2450
CTEPRGVPSP KKPEASNRVA ILLGMLMPTV LLVPAVTRVS RKRRRRRRPS
2460 2470
RERTQSQNRG KRAGTDCAPE QAYKVA
Length:2,476
Mass (Da):270,365
Last modified:November 1, 1996 - v1
Checksum:iA13B690375A6548C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti823 – 8231C → V AA sequence (PubMed:7592795)Curated
Sequence conflicti923 – 9231S → Y AA sequence (PubMed:7592795)Curated
Sequence conflicti965 – 9651W → Y AA sequence (PubMed:7592795)Curated
Sequence conflicti1241 – 12411S → K AA sequence (PubMed:7592795)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40024 mRNA. Translation: AAC48486.1.
PIRiT34022.
RefSeqiNP_999548.1. NM_214383.1.
UniGeneiSsc.82465.

Genome annotation databases

GeneIDi397676.
KEGGissc:397676.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40024 mRNA. Translation: AAC48486.1 .
PIRi T34022.
RefSeqi NP_999548.1. NM_214383.1.
UniGenei Ssc.82465.

3D structure databases

ProteinModelPortali Q28983.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9823.ENSSSCP00000008195.

Proteomic databases

PRIDEi Q28983.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 397676.
KEGGi ssc:397676.

Organism-specific databases

CTDi 7455.

Phylogenomic databases

eggNOGi NOG12793.
HOGENOMi HOG000168448.
HOVERGENi HBG000375.
InParanoidi Q28983.

Family and domain databases

InterProi IPR013320. ConA-like_dom.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000998. MAM_dom.
IPR002919. TIL_dom.
IPR025615. TILa_dom.
IPR014853. Unchr_dom_Cys-rich.
IPR001007. VWF_C.
IPR001846. VWF_type-D.
[Graphical view ]
Pfami PF08742. C8. 4 hits.
PF00008. EGF. 1 hit.
PF00629. MAM. 2 hits.
PF01826. TIL. 5 hits.
PF12714. TILa. 5 hits.
PF00094. VWD. 4 hits.
[Graphical view ]
SMARTi SM00832. C8. 4 hits.
SM00181. EGF. 1 hit.
SM00137. MAM. 1 hit.
SM00214. VWC. 2 hits.
SM00216. VWD. 4 hits.
[Graphical view ]
SUPFAMi SSF49899. SSF49899. 2 hits.
SSF57567. SSF57567. 5 hits.
PROSITEi PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 4 hits.
PS50026. EGF_3. 1 hit.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 2 hits.
PS51233. VWFD. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A sperm membrane protein that binds in a species-specific manner to the egg extracellular matrix is homologous to von Willebrand factor."
    Hardy D.M., Garbers D.L.
    J. Biol. Chem. 270:26025-26028(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 823-830; 859-872; 883-890; 920-925; 960-967; 1235-1244; 1349-1354; 1518-1532; 1624-1656; 1658-1667; 1777-1795 AND 1914-1921.
    Strain: Meishan.
    Tissue: Testis.

Entry informationi

Entry nameiZAN_PIG
AccessioniPrimary (citable) accession number: Q28983
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3