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Q28949

- MA2B2_PIG

UniProt

Q28949 - MA2B2_PIG

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Protein

Epididymis-specific alpha-mannosidase

Gene

MAN2B2

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Can digest both p-nitro-phenyl-alpha-D-mannoside and high mannose oligosaccharide (Man(8)-GlcNAc2). May be involved in sperm maturation. Has a possible role in specific sperm-egg interaction since sperm surface mannosidase acts like a receptor for mannose-containing oligosaccharides located on the zona pellucida.

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Binds 1 zinc ion per subunit.By similarity

pH dependencei

Optimum pH is 6.5.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi36 – 361ZincBy similarity
Metal bindingi38 – 381ZincBy similarity
Active sitei151 – 1511NucleophileBy similarity
Metal bindingi151 – 1511ZincBy similarity
Metal bindingi411 – 4111ZincBy similarity

GO - Molecular functioni

  1. alpha-mannosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. mannose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

CAZyiGH38. Glycoside Hydrolase Family 38.

Names & Taxonomyi

Protein namesi
Recommended name:
Epididymis-specific alpha-mannosidase (EC:3.2.1.24)
Alternative name(s):
Mannosidase alpha class 2B member 2
Gene namesi
Name:MAN2B2
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Unplaced

Subcellular locationi

Secreted
Note: Found at the sperm surface as a 27 kDa fragment.

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Add
BLAST
Chaini22 – 995974Epididymis-specific alpha-mannosidasePRO_0000012079Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi285 – 2851N-linked (GlcNAc...)Sequence Analysis
Glycosylationi593 – 5931N-linked (GlcNAc...)Sequence Analysis
Glycosylationi625 – 6251N-linked (GlcNAc...)Sequence Analysis
Glycosylationi657 – 6571N-linked (GlcNAc...)Sequence Analysis
Glycosylationi733 – 7331N-linked (GlcNAc...)Sequence Analysis
Glycosylationi793 – 7931N-linked (GlcNAc...)Sequence Analysis
Glycosylationi875 – 8751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi977 – 9771N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Processed into a 27 kDa fragment localized on the equatorial segment and the apical rim of the head of mature sperm.

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Specific to the caput and corpus of the epididymis.

Structurei

3D structure databases

ProteinModelPortaliQ28949.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 38 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG052392.
InParanoidiQ28949.
KOiK12312.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q28949-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGPHSWLPLF MQLALLGPQW ALHFYKVKVF VVPHSHMDVG WLHTVQESMQ
60 70 80 90 100
VYVPDVYNSV VEALTRGKRR RFIAVEQEYF RLWWDGFASA KRKQQVRQLL
110 120 130 140 150
AEQRLEFVLG GQVMHDEAVT HFDDQILQLT EGHGFLYETF GIRPQFSWQV
160 170 180 190 200
DPFGASATTP TLLALAGFNG HIISRIDYDL KDTMQHTQGL QFVWRGSRSL
210 220 230 240 250
EARQEIFTHV LDQYSYCSDG FMWNGSPRFP DRPFDMDYSA VEMPVSQDSM
260 270 280 290 300
NHYVLNLVDN VNKRAAWFRT QHVLWPWGCD RQFFNASQQF ANMDRLMDHI
310 320 330 340 350
NKHTPELGIS MQYATLAEYF QAVFAQDVSW QVRDHRDFLP YSSAPEQTWT
360 370 380 390 400
GFYTSQSGLK RLARRASALL YAGESLFTRY MLSAAHRFLD PAWALTQLQQ
410 420 430 440 450
LRWAVSEVQH HDGITGTHIL AVRDMFVEHL TTGMAGVRKL MDSIAQDMPL
460 470 480 490 500
THSGPEPGGH VAMVYNPLAW TVTTVITLTV SFPEVSVTDE SGRPVLAQVQ
510 520 530 540 550
DSKETPSAYD LHVLTTIPGL SYQHYIIKPI RKAREDSQEA AATVPSTIQF
560 570 580 590 600
GLKLRRQDGQ VGRNLVPVKN SCYTVFLDKD TNLMHSIWER QSNRTVRMSQ
610 620 630 640 650
EFLAYRSVYG YEEAVTSDNY LFTPNGTAEP AWAAVRMEVV EGQLLSEIRQ
660 670 680 690 700
YFYRQANDSD HTYAIYSRLA HGPQDSAGEL LCHRIEQEYR VGPLELNHEV
710 720 730 740 750
VLRTSTSLNT GLVLYSDNNG YQMQRRTYRH DRNNSVSLNY YPMAQSAFIQ
760 770 780 790 800
DGGSRLVLLS EQAHGVSSQG NGQVEVMLHR RLWNKLEWTL QYNLTHDVTS
810 820 830 840 850
VVRPVLWLLL GPRTLTTGLR QRSGLELQHR PVVLFRELGG TVQNGPGPRK
860 870 880 890 900
QEPVTLPPSL HLQILSIPGW KYSSNHTVHL KNLQKGHYRR AKADFRRVLL
910 920 930 940 950
RLHHLYEAGE HQALSRPVTL NLQSVLRGLG SVVAVEERSL TGTWDVNSLH
960 970 980 990
RWSWKTEDGH HHRGSSRRPL PPLRGPNVTI HPKEIRTFFI HFQEQ
Length:995
Mass (Da):114,230
Last modified:November 16, 2001 - v2
Checksum:i79D21B8CE5AE6FC2
GO

Sequence cautioni

The sequence BAA05877.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti945 – 9451D → A AA sequence (PubMed:8562059)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D28521 mRNA. Translation: BAA05877.1. Different initiation.
PIRiT42762.
RefSeqiNP_999014.1. NM_213849.1.
UniGeneiSsc.14529.

Genome annotation databases

GeneIDi396847.
KEGGissc:396847.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D28521 mRNA. Translation: BAA05877.1 . Different initiation.
PIRi T42762.
RefSeqi NP_999014.1. NM_213849.1.
UniGenei Ssc.14529.

3D structure databases

ProteinModelPortali Q28949.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH38. Glycoside Hydrolase Family 38.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 396847.
KEGGi ssc:396847.

Organism-specific databases

CTDi 23324.

Phylogenomic databases

HOVERGENi HBG052392.
InParanoidi Q28949.
KOi K12312.

Family and domain databases

Gene3Di 1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view ]
Pfami PF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view ]
SMARTi SM00872. Alpha-mann_mid. 1 hit.
[Graphical view ]
SUPFAMi SSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning of complementary DNA encoding a 135-kilodalton protein secreted from porcine corpus epididymis and its identification as an epididymis-specific alpha-mannosidase."
    Okamura N., Tamba M., Liao H.-J., Onoe S., Sugita Y., Dacheux F., Dacheux J.-L.
    Mol. Reprod. Dev. 42:141-148(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Epididymis.

Entry informationi

Entry nameiMA2B2_PIG
AccessioniPrimary (citable) accession number: Q28949
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 16, 2001
Last modified: October 29, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3