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Q28949

- MA2B2_PIG

UniProt

Q28949 - MA2B2_PIG

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Protein
Epididymis-specific alpha-mannosidase
Gene
MAN2B2
Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Can digest both p-nitro-phenyl-alpha-D-mannoside and high mannose oligosaccharide (Man(8)-GlcNAc2). May be involved in sperm maturation. Has a possible role in specific sperm-egg interaction since sperm surface mannosidase acts like a receptor for mannose-containing oligosaccharides located on the zona pellucida.

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Binds 1 zinc ion per subunit By similarity.

pH dependencei

Optimum pH is 6.5.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi36 – 361Zinc By similarity
Metal bindingi38 – 381Zinc By similarity
Active sitei151 – 1511Nucleophile By similarity
Metal bindingi151 – 1511Zinc By similarity
Metal bindingi411 – 4111Zinc By similarity

GO - Molecular functioni

  1. alpha-mannosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. mannose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

CAZyiGH38. Glycoside Hydrolase Family 38.

Names & Taxonomyi

Protein namesi
Recommended name:
Epididymis-specific alpha-mannosidase (EC:3.2.1.24)
Alternative name(s):
Mannosidase alpha class 2B member 2
Gene namesi
Name:MAN2B2
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Unplaced

Subcellular locationi

Secreted
Note: Found at the sperm surface as a 27 kDa fragment.

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121
Add
BLAST
Chaini22 – 995974Epididymis-specific alpha-mannosidase
PRO_0000012079Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi285 – 2851N-linked (GlcNAc...) Reviewed prediction
Glycosylationi593 – 5931N-linked (GlcNAc...) Reviewed prediction
Glycosylationi625 – 6251N-linked (GlcNAc...) Reviewed prediction
Glycosylationi657 – 6571N-linked (GlcNAc...) Reviewed prediction
Glycosylationi733 – 7331N-linked (GlcNAc...) Reviewed prediction
Glycosylationi793 – 7931N-linked (GlcNAc...) Reviewed prediction
Glycosylationi875 – 8751N-linked (GlcNAc...) Reviewed prediction
Glycosylationi977 – 9771N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Processed into a 27 kDa fragment localized on the equatorial segment and the apical rim of the head of mature sperm.

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Specific to the caput and corpus of the epididymis.

Structurei

3D structure databases

ProteinModelPortaliQ28949.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG052392.
KOiK12312.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q28949-1 [UniParc]FASTAAdd to Basket

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MGPHSWLPLF MQLALLGPQW ALHFYKVKVF VVPHSHMDVG WLHTVQESMQ    50
VYVPDVYNSV VEALTRGKRR RFIAVEQEYF RLWWDGFASA KRKQQVRQLL 100
AEQRLEFVLG GQVMHDEAVT HFDDQILQLT EGHGFLYETF GIRPQFSWQV 150
DPFGASATTP TLLALAGFNG HIISRIDYDL KDTMQHTQGL QFVWRGSRSL 200
EARQEIFTHV LDQYSYCSDG FMWNGSPRFP DRPFDMDYSA VEMPVSQDSM 250
NHYVLNLVDN VNKRAAWFRT QHVLWPWGCD RQFFNASQQF ANMDRLMDHI 300
NKHTPELGIS MQYATLAEYF QAVFAQDVSW QVRDHRDFLP YSSAPEQTWT 350
GFYTSQSGLK RLARRASALL YAGESLFTRY MLSAAHRFLD PAWALTQLQQ 400
LRWAVSEVQH HDGITGTHIL AVRDMFVEHL TTGMAGVRKL MDSIAQDMPL 450
THSGPEPGGH VAMVYNPLAW TVTTVITLTV SFPEVSVTDE SGRPVLAQVQ 500
DSKETPSAYD LHVLTTIPGL SYQHYIIKPI RKAREDSQEA AATVPSTIQF 550
GLKLRRQDGQ VGRNLVPVKN SCYTVFLDKD TNLMHSIWER QSNRTVRMSQ 600
EFLAYRSVYG YEEAVTSDNY LFTPNGTAEP AWAAVRMEVV EGQLLSEIRQ 650
YFYRQANDSD HTYAIYSRLA HGPQDSAGEL LCHRIEQEYR VGPLELNHEV 700
VLRTSTSLNT GLVLYSDNNG YQMQRRTYRH DRNNSVSLNY YPMAQSAFIQ 750
DGGSRLVLLS EQAHGVSSQG NGQVEVMLHR RLWNKLEWTL QYNLTHDVTS 800
VVRPVLWLLL GPRTLTTGLR QRSGLELQHR PVVLFRELGG TVQNGPGPRK 850
QEPVTLPPSL HLQILSIPGW KYSSNHTVHL KNLQKGHYRR AKADFRRVLL 900
RLHHLYEAGE HQALSRPVTL NLQSVLRGLG SVVAVEERSL TGTWDVNSLH 950
RWSWKTEDGH HHRGSSRRPL PPLRGPNVTI HPKEIRTFFI HFQEQ 995
Length:995
Mass (Da):114,230
Last modified:November 16, 2001 - v2
Checksum:i79D21B8CE5AE6FC2
GO

Sequence cautioni

The sequence BAA05877.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti945 – 9451D → A AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D28521 mRNA. Translation: BAA05877.1. Different initiation.
PIRiT42762.
RefSeqiNP_999014.1. NM_213849.1.
UniGeneiSsc.14529.

Genome annotation databases

GeneIDi396847.
KEGGissc:396847.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D28521 mRNA. Translation: BAA05877.1 . Different initiation.
PIRi T42762.
RefSeqi NP_999014.1. NM_213849.1.
UniGenei Ssc.14529.

3D structure databases

ProteinModelPortali Q28949.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH38. Glycoside Hydrolase Family 38.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 396847.
KEGGi ssc:396847.

Organism-specific databases

CTDi 23324.

Phylogenomic databases

HOVERGENi HBG052392.
KOi K12312.

Family and domain databases

Gene3Di 1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view ]
Pfami PF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view ]
SMARTi SM00872. Alpha-mann_mid. 1 hit.
[Graphical view ]
SUPFAMi SSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning of complementary DNA encoding a 135-kilodalton protein secreted from porcine corpus epididymis and its identification as an epididymis-specific alpha-mannosidase."
    Okamura N., Tamba M., Liao H.-J., Onoe S., Sugita Y., Dacheux F., Dacheux J.-L.
    Mol. Reprod. Dev. 42:141-148(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Epididymis.

Entry informationi

Entry nameiMA2B2_PIG
AccessioniPrimary (citable) accession number: Q28949
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 16, 2001
Last modified: June 11, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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