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Q28949

- MA2B2_PIG

UniProt

Q28949 - MA2B2_PIG

Protein

Epididymis-specific alpha-mannosidase

Gene

MAN2B2

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 2 (16 Nov 2001)
      Previous versions | rss
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    Functioni

    Can digest both p-nitro-phenyl-alpha-D-mannoside and high mannose oligosaccharide (Man(8)-GlcNAc2). May be involved in sperm maturation. Has a possible role in specific sperm-egg interaction since sperm surface mannosidase acts like a receptor for mannose-containing oligosaccharides located on the zona pellucida.

    Catalytic activityi

    Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    pH dependencei

    Optimum pH is 6.5.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi36 – 361ZincBy similarity
    Metal bindingi38 – 381ZincBy similarity
    Active sitei151 – 1511NucleophileBy similarity
    Metal bindingi151 – 1511ZincBy similarity
    Metal bindingi411 – 4111ZincBy similarity

    GO - Molecular functioni

    1. alpha-mannosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. mannose metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    CAZyiGH38. Glycoside Hydrolase Family 38.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Epididymis-specific alpha-mannosidase (EC:3.2.1.24)
    Alternative name(s):
    Mannosidase alpha class 2B member 2
    Gene namesi
    Name:MAN2B2
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Unplaced

    Subcellular locationi

    Secreted
    Note: Found at the sperm surface as a 27 kDa fragment.

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Add
    BLAST
    Chaini22 – 995974Epididymis-specific alpha-mannosidasePRO_0000012079Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi285 – 2851N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi593 – 5931N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi625 – 6251N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi657 – 6571N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi733 – 7331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi793 – 7931N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi875 – 8751N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi977 – 9771N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Processed into a 27 kDa fragment localized on the equatorial segment and the apical rim of the head of mature sperm.

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Tissue specificityi

    Specific to the caput and corpus of the epididymis.

    Structurei

    3D structure databases

    ProteinModelPortaliQ28949.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 38 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOVERGENiHBG052392.
    KOiK12312.

    Family and domain databases

    Gene3Di1.20.1270.50. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.110.10. 1 hit.
    InterProiIPR011013. Gal_mutarotase_SF_dom.
    IPR011330. Glyco_hydro/deAcase_b/a-brl.
    IPR013780. Glyco_hydro_13_b.
    IPR027291. Glyco_hydro_38/57_N.
    IPR011682. Glyco_hydro_38_C.
    IPR015341. Glyco_hydro_38_cen.
    IPR000602. Glyco_hydro_38_N.
    IPR028995. Glyco_hydro_57/38_cen.
    [Graphical view]
    PfamiPF09261. Alpha-mann_mid. 1 hit.
    PF01074. Glyco_hydro_38. 1 hit.
    PF07748. Glyco_hydro_38C. 1 hit.
    [Graphical view]
    SMARTiSM00872. Alpha-mann_mid. 1 hit.
    [Graphical view]
    SUPFAMiSSF74650. SSF74650. 1 hit.
    SSF88688. SSF88688. 1 hit.
    SSF88713. SSF88713. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q28949-1 [UniParc]FASTAAdd to Basket

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    MGPHSWLPLF MQLALLGPQW ALHFYKVKVF VVPHSHMDVG WLHTVQESMQ    50
    VYVPDVYNSV VEALTRGKRR RFIAVEQEYF RLWWDGFASA KRKQQVRQLL 100
    AEQRLEFVLG GQVMHDEAVT HFDDQILQLT EGHGFLYETF GIRPQFSWQV 150
    DPFGASATTP TLLALAGFNG HIISRIDYDL KDTMQHTQGL QFVWRGSRSL 200
    EARQEIFTHV LDQYSYCSDG FMWNGSPRFP DRPFDMDYSA VEMPVSQDSM 250
    NHYVLNLVDN VNKRAAWFRT QHVLWPWGCD RQFFNASQQF ANMDRLMDHI 300
    NKHTPELGIS MQYATLAEYF QAVFAQDVSW QVRDHRDFLP YSSAPEQTWT 350
    GFYTSQSGLK RLARRASALL YAGESLFTRY MLSAAHRFLD PAWALTQLQQ 400
    LRWAVSEVQH HDGITGTHIL AVRDMFVEHL TTGMAGVRKL MDSIAQDMPL 450
    THSGPEPGGH VAMVYNPLAW TVTTVITLTV SFPEVSVTDE SGRPVLAQVQ 500
    DSKETPSAYD LHVLTTIPGL SYQHYIIKPI RKAREDSQEA AATVPSTIQF 550
    GLKLRRQDGQ VGRNLVPVKN SCYTVFLDKD TNLMHSIWER QSNRTVRMSQ 600
    EFLAYRSVYG YEEAVTSDNY LFTPNGTAEP AWAAVRMEVV EGQLLSEIRQ 650
    YFYRQANDSD HTYAIYSRLA HGPQDSAGEL LCHRIEQEYR VGPLELNHEV 700
    VLRTSTSLNT GLVLYSDNNG YQMQRRTYRH DRNNSVSLNY YPMAQSAFIQ 750
    DGGSRLVLLS EQAHGVSSQG NGQVEVMLHR RLWNKLEWTL QYNLTHDVTS 800
    VVRPVLWLLL GPRTLTTGLR QRSGLELQHR PVVLFRELGG TVQNGPGPRK 850
    QEPVTLPPSL HLQILSIPGW KYSSNHTVHL KNLQKGHYRR AKADFRRVLL 900
    RLHHLYEAGE HQALSRPVTL NLQSVLRGLG SVVAVEERSL TGTWDVNSLH 950
    RWSWKTEDGH HHRGSSRRPL PPLRGPNVTI HPKEIRTFFI HFQEQ 995
    Length:995
    Mass (Da):114,230
    Last modified:November 16, 2001 - v2
    Checksum:i79D21B8CE5AE6FC2
    GO

    Sequence cautioni

    The sequence BAA05877.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti945 – 9451D → A AA sequence (PubMed:8562059)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D28521 mRNA. Translation: BAA05877.1. Different initiation.
    PIRiT42762.
    RefSeqiNP_999014.1. NM_213849.1.
    UniGeneiSsc.14529.

    Genome annotation databases

    GeneIDi396847.
    KEGGissc:396847.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D28521 mRNA. Translation: BAA05877.1 . Different initiation.
    PIRi T42762.
    RefSeqi NP_999014.1. NM_213849.1.
    UniGenei Ssc.14529.

    3D structure databases

    ProteinModelPortali Q28949.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH38. Glycoside Hydrolase Family 38.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 396847.
    KEGGi ssc:396847.

    Organism-specific databases

    CTDi 23324.

    Phylogenomic databases

    HOVERGENi HBG052392.
    KOi K12312.

    Family and domain databases

    Gene3Di 1.20.1270.50. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.110.10. 1 hit.
    InterProi IPR011013. Gal_mutarotase_SF_dom.
    IPR011330. Glyco_hydro/deAcase_b/a-brl.
    IPR013780. Glyco_hydro_13_b.
    IPR027291. Glyco_hydro_38/57_N.
    IPR011682. Glyco_hydro_38_C.
    IPR015341. Glyco_hydro_38_cen.
    IPR000602. Glyco_hydro_38_N.
    IPR028995. Glyco_hydro_57/38_cen.
    [Graphical view ]
    Pfami PF09261. Alpha-mann_mid. 1 hit.
    PF01074. Glyco_hydro_38. 1 hit.
    PF07748. Glyco_hydro_38C. 1 hit.
    [Graphical view ]
    SMARTi SM00872. Alpha-mann_mid. 1 hit.
    [Graphical view ]
    SUPFAMi SSF74650. SSF74650. 1 hit.
    SSF88688. SSF88688. 1 hit.
    SSF88713. SSF88713. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of complementary DNA encoding a 135-kilodalton protein secreted from porcine corpus epididymis and its identification as an epididymis-specific alpha-mannosidase."
      Okamura N., Tamba M., Liao H.-J., Onoe S., Sugita Y., Dacheux F., Dacheux J.-L.
      Mol. Reprod. Dev. 42:141-148(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Epididymis.

    Entry informationi

    Entry nameiMA2B2_PIG
    AccessioniPrimary (citable) accession number: Q28949
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 16, 2001
    Last modified: October 1, 2014
    This is version 89 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3