Reviewed, UniProtKB/Swiss-Prot Q28944 (CATL1_PIG)

Names and origin

Protein names

Recommended name:
    Cathepsin L1
    EC=3.4.22.15
Cleaved into the following 2 chains:
    1- Recommended name:
            Cathepsin L1 heavy chain
    2- Recommended name:
            Cathepsin L1 light chain

Gene names

Name:	CTSL1
Synonyms:	CTSL

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Important for the overall degradation of proteins in lysosomes.
Catalytic activity	Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.
Subunit structure	Dimer of a heavy and a light chain linked by disulfide bonds.
Subcellular location	Lysosome.
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Propeptide	18 – 117	100	Activation peptide		PRO_0000026252
	Chain	118 – 289	172	Cathepsin L1 heavy chain		PRO_0000026253
	Propeptide	290 – 291	2	By similarity		PRO_0000026254
	Chain	292 – 334	43	Cathepsin L1 light chain		PRO_0000026255
Sites
	Active site	138	1	By similarity
	Active site	277	1	By similarity
	Active site	301	1	By similarity
Amino acid modifications
	Glycosylation	222	1	N-linked (GlcNAc...) Potential
	Glycosylation	292	1	N-linked (GlcNAc...) Potential
	Disulfide bond	135 ↔ 178		By similarity
	Disulfide bond	169 ↔ 212		By similarity
	Disulfide bond	270 ↔ 323		Interchain (between heavy and light chains) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q28944-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 51DBA79ACCF2CE53	FASTA	334	37,178
	10 20 30 40 50 60 MKPSLFLTAL CLGIASAAPK LDQNLDADWY KWKATHGRLY GMNEEGWRRA VWEKNMKMIE 70 80 90 100 110 120 LHNQEYSQGK HGFSMAMNAF GDMTNEEFRQ VMNGFQNQKH KKGKVFHESL VLEVPKSVDW 130 140 150 160 170 180 REKGYVTAVK NQGQCGSCWA FSATGALEGQ MFRKTGKLVS LSEQNLVDCS RPQGNQGCNG 190 200 210 220 230 240 GLMDNAFQYV KDNGGLDTEE SYPYLGRETN SCTYKPECSA ANDTGFVDIP QREKALMKAV 250 260 270 280 290 300 ATVGPISVAI DAGHSSFQFY KSGIYYDPDC SSKDLDHGVL VVGYGFEGTD SNSSKFWIVK 310 320 330 NSWGPEWGWN GYVKMAKDQN NHCGISTAAS YPTV

References

[1]	"Direct evidence for the elevated synthesis and secretion of procathepsin L in the distal caput epididymis of boar." Okamura N., Tamba M., Uchiyama Y., Sugita Y., Dacheux F., Syntin P., Dacheux J.-L. Biochim. Biophys. Acta 1245:221-226(1995) [PubMed: 7492581] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Epididymis.
[2]	"Characterization and comparative mapping of the porcine CTSL gene indicates a novel synteny between HSA9q21-->q22 and SSC10q11-->q12." Spoetter A., Droegemueller C., Kuiper H., Brenig B., Leeb T., Distl O. Cytogenet. Cell Genet. 95:92-96(2001) [PubMed: 11978977] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	D37917 mRNA. Translation: BAA07140.1. AJ315771 Genomic DNA. Translation: CAC44793.1.
PIR	A58195.
RefSeq	NP_999057.1.
UniGene	Ssc.54036
3D structure databases
HSSP	HSSP built from PDB template 1ICF based on UniProtKB P07711.
SMR	Q28944. Positions 21-334.
ModBase	Search...
Protein family/group databases
MEROPS	C01.032.
Genome annotation databases
GeneID	396926.
KEGG	ssc:396926.
Organism-specific databases
CTD	396926.
Phylogenomic databases
HOVERGEN	Q28944.
Enzyme and pathway databases
BRENDA	3.4.22.15. 249.
Family and domain databases
InterPro	IPR000169. Pept_cys_AS. IPR013128. Peptidase_C1A. IPR000668. Peptidase_C1A_C. IPR013201. Prot_inhib_I29. [Graphical view]
PANTHER	PTHR12411. Peptidase_C1A. 1 hit.
Pfam	PF08246. Inhibitor_I29. 1 hit. PF00112. Peptidase_C1. 1 hit. [Graphical view]
PRINTS	PR00705. PAPAIN.
ProDom	PD000158. Peptidase_C1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00645. Pept_C1. 1 hit. [Graphical view]
PROSITE	PS00640. THIOL_PROTEASE_ASN. 1 hit. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CATL1_PIG

Accession

Primary (citable) accession number: Q28944

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	September 1, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)