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Protein

Tyrosine-protein kinase Yes

Gene

YES1

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis (By similarity).By similarity

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei301 – 3011ATPPROSITE-ProRule annotation
Active sitei392 – 3921Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi279 – 2879ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 1154.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase Yes (EC:2.7.10.2)
Alternative name(s):
Proto-oncogene c-Yes
p61-Yes
Gene namesi
Name:YES1
Synonyms:YES
OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
ProteomesiUP000002254: Unplaced

Subcellular locationi

Cell membrane By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cytoplasmcytosol By similarity
Note: Newly synthesized protein initially accumulates in the Golgi region and traffics to the plasma membrane through the exocytic pathway.By similarity

GO - Cellular componenti

  1. cytosol Source: UniProtKB-SubCell
  2. microtubule organizing center Source: UniProtKB-SubCell
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 539538Tyrosine-protein kinase YesPRO_0000088180Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Lipidationi3 – 31S-palmitoyl cysteine; in membrane formBy similarity
Modified residuei19 – 191PhosphothreonineBy similarity
Modified residuei28 – 281PhosphotyrosineBy similarity
Modified residuei107 – 1071PhosphoserineBy similarity
Modified residuei190 – 1901PhosphotyrosineBy similarity
Modified residuei191 – 1911PhosphoserineBy similarity
Modified residuei218 – 2181PhosphotyrosineBy similarity
Modified residuei219 – 2191PhosphotyrosineBy similarity
Modified residuei332 – 3321PhosphotyrosineBy similarity
Modified residuei341 – 3411PhosphotyrosineBy similarity
Modified residuei422 – 4221Phosphotyrosine; by autocatalysisBy similarity
Modified residuei533 – 5331Phosphotyrosine; by CSKBy similarity

Post-translational modificationi

Phosphorylation by CSK on the C-terminal tail maintains the enzyme in an inactive state. Autophosphorylation at Tyr-422 maintains enzyme activity by blocking CSK-mediated inhibition (By similarity).By similarity
Palmitoylation at Cys-3 promotes membrane localization.By similarity

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

PRIDEiQ28923.

Interactioni

Subunit structurei

Interacts with YAP1. Interacts with FASLG. Interacts with CTNND1; this interaction allows YES1-mediated activation of FYN and FER and subsequent phosphorylation of CTNND1. Interacts with CSF1R (By similarity).By similarity

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000027127.

Structurei

3D structure databases

ProteinModelPortaliQ28923.
SMRiQ28923. Positions 89-539.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini87 – 14862SH3PROSITE-ProRule annotationAdd
BLAST
Domaini154 – 25198SH2PROSITE-ProRule annotationAdd
BLAST
Domaini273 – 526254Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233858.
HOVERGENiHBG008761.
InParanoidiQ28923.
KOiK05705.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q28923-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCIKSKEDK GPAIKYRNTP EPVSVSHYGA EPTQATPWPS SSGWGTAFNF
60 70 80 90 100
SSLSMTGFGG SSGVTPFGGA SSSFSVVPSP YPAGLTGGVT IFVALYDYEA
110 120 130 140 150
RTTEDLSFKG GERFQIINNT EGDWWEARSI ATGKNGYIPS NYVAPADSIA
160 170 180 190 200
AEEWYFGKMG RKDAERLLLN PGNQRGIFLV RESETTKGAY SLSIRDWDEI
210 220 230 240 250
RGDNVKHYKI RKLDNGGYYI TTRAQFDTLQ KLVKHSTEHA DGLCHKLTTV
260 270 280 290 300
CPTVKPQTQG LAKDAWEIPR ESLRLEVKLG QGCFGEVWMG TWNGTTKVAI
310 320 330 340 350
KTLKLGTMMP EAFLQEAQIM KKLRHDKLVP LYAVVSEEPI YIVTEFMSKG
360 370 380 390 400
SLLDFLKEGD GKYLKLPQLV DMAAQIADGM AYIERMNYIH RDLRAANILV
410 420 430 440 450
GENLVCKIAD FGLARLIEDN EYTARQGAKF PIKWTAPEAA LYGRFTIKSD
460 470 480 490 500
VWSFGILQTE LTTKGRVPYP GMVNREVLEQ VERGYRMPCP QGCPESLHEL
510 520 530
MNLCWKKDPD ERPTFEYIQS FLEDYFTAAE PQYQPGENL
Length:539
Mass (Da):60,300
Last modified:January 23, 2007 - v3
Checksum:iCC4BEA38073CC106
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S81472 mRNA. Translation: AAB36132.1.
RefSeqiNP_001003239.1. NM_001003239.1.
UniGeneiCfa.3778.

Genome annotation databases

GeneIDi403917.
KEGGicfa:403917.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S81472 mRNA. Translation: AAB36132.1.
RefSeqiNP_001003239.1. NM_001003239.1.
UniGeneiCfa.3778.

3D structure databases

ProteinModelPortaliQ28923.
SMRiQ28923. Positions 89-539.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000027127.

Proteomic databases

PRIDEiQ28923.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi403917.
KEGGicfa:403917.

Organism-specific databases

CTDi7525.

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233858.
HOVERGENiHBG008761.
InParanoidiQ28923.
KOiK05705.

Enzyme and pathway databases

BRENDAi2.7.10.2. 1154.

Miscellaneous databases

NextBioi20817409.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Spleen.
  2. "Nonreceptor tyrosine kinase c-Yes interacts with occludin during tight junction formation in canine kidney epithelial cells."
    Chen Y.H., Lu Q., Goodenough D.A., Jeansonne B.
    Mol. Biol. Cell 13:1227-1237(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH OCLLUDIN/OCLN, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiYES_CANFA
AccessioniPrimary (citable) accession number: Q28923
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2007
Last modified: March 4, 2015
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.