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Q28923

- YES_CANFA

UniProt

Q28923 - YES_CANFA

Protein

Tyrosine-protein kinase Yes

Gene

YES1

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis By similarity.By similarity

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei301 – 3011ATPPROSITE-ProRule annotation
    Active sitei392 – 3921Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi279 – 2879ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.10.2. 1154.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein kinase Yes (EC:2.7.10.2)
    Alternative name(s):
    Proto-oncogene c-Yes
    p61-Yes
    Gene namesi
    Name:YES1
    Synonyms:YES
    OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
    Taxonomic identifieri9615 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
    ProteomesiUP000002254: Unplaced

    Subcellular locationi

    Cell membrane By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cytoplasmcytosol By similarity
    Note: Newly synthesized protein initially accumulates in the Golgi region and traffics to the plasma membrane through the exocytic pathway.By similarity

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell
    2. microtubule organizing center Source: UniProtKB-SubCell
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Cytoskeleton, Membrane

    Pathology & Biotechi

    Keywords - Diseasei

    Proto-oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 539538Tyrosine-protein kinase YesPRO_0000088180Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineBy similarity
    Lipidationi3 – 31S-palmitoyl cysteine; in membrane formBy similarity
    Modified residuei19 – 191PhosphothreonineBy similarity
    Modified residuei28 – 281PhosphotyrosineBy similarity
    Modified residuei107 – 1071PhosphoserineBy similarity
    Modified residuei190 – 1901PhosphotyrosineBy similarity
    Modified residuei191 – 1911PhosphoserineBy similarity
    Modified residuei218 – 2181PhosphotyrosineBy similarity
    Modified residuei219 – 2191PhosphotyrosineBy similarity
    Modified residuei332 – 3321PhosphotyrosineBy similarity
    Modified residuei341 – 3411PhosphotyrosineBy similarity
    Modified residuei422 – 4221Phosphotyrosine; by autocatalysisBy similarity
    Modified residuei442 – 4421PhosphotyrosineBy similarity
    Modified residuei533 – 5331Phosphotyrosine; by CSKBy similarity

    Post-translational modificationi

    Phosphorylation by CSK on the C-terminal tail maintains the enzyme in an inactive state. Autophosphorylation at Tyr-422 maintains enzyme activity by blocking CSK-mediated inhibition By similarity.By similarity
    Palmitoylation at Cys-3 promotes membrane localization.By similarity

    Keywords - PTMi

    Lipoprotein, Myristate, Palmitate, Phosphoprotein

    Proteomic databases

    PRIDEiQ28923.

    Interactioni

    Subunit structurei

    Interacts with YAP1. Interacts with FASLG. Interacts with CTNND1; this interaction allows YES1-mediated activation of FYN and FER and subsequent phosphorylation of CTNND1. Interacts with CSF1R By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9615.ENSCAFP00000027127.

    Structurei

    3D structure databases

    ProteinModelPortaliQ28923.
    SMRiQ28923. Positions 89-539.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini87 – 14862SH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini154 – 25198SH2PROSITE-ProRule annotationAdd
    BLAST
    Domaini273 – 526254Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 SH2 domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH2 domain, SH3 domain

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233858.
    HOVERGENiHBG008761.
    InParanoidiQ28923.
    KOiK05705.

    Family and domain databases

    Gene3Di3.30.505.10. 1 hit.
    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR001452. SH3_domain.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view]
    PfamiPF07714. Pkinase_Tyr. 1 hit.
    PF00017. SH2. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view]
    PRINTSiPR00401. SH2DOMAIN.
    PR00452. SH3DOMAIN.
    PR00109. TYRKINASE.
    SMARTiSM00252. SH2. 1 hit.
    SM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    SSF55550. SSF55550. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50001. SH2. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q28923-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGCIKSKEDK GPAIKYRNTP EPVSVSHYGA EPTQATPWPS SSGWGTAFNF    50
    SSLSMTGFGG SSGVTPFGGA SSSFSVVPSP YPAGLTGGVT IFVALYDYEA 100
    RTTEDLSFKG GERFQIINNT EGDWWEARSI ATGKNGYIPS NYVAPADSIA 150
    AEEWYFGKMG RKDAERLLLN PGNQRGIFLV RESETTKGAY SLSIRDWDEI 200
    RGDNVKHYKI RKLDNGGYYI TTRAQFDTLQ KLVKHSTEHA DGLCHKLTTV 250
    CPTVKPQTQG LAKDAWEIPR ESLRLEVKLG QGCFGEVWMG TWNGTTKVAI 300
    KTLKLGTMMP EAFLQEAQIM KKLRHDKLVP LYAVVSEEPI YIVTEFMSKG 350
    SLLDFLKEGD GKYLKLPQLV DMAAQIADGM AYIERMNYIH RDLRAANILV 400
    GENLVCKIAD FGLARLIEDN EYTARQGAKF PIKWTAPEAA LYGRFTIKSD 450
    VWSFGILQTE LTTKGRVPYP GMVNREVLEQ VERGYRMPCP QGCPESLHEL 500
    MNLCWKKDPD ERPTFEYIQS FLEDYFTAAE PQYQPGENL 539
    Length:539
    Mass (Da):60,300
    Last modified:January 23, 2007 - v3
    Checksum:iCC4BEA38073CC106
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S81472 mRNA. Translation: AAB36132.1.
    RefSeqiNP_001003239.1. NM_001003239.1.
    UniGeneiCfa.3778.

    Genome annotation databases

    GeneIDi403917.
    KEGGicfa:403917.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S81472 mRNA. Translation: AAB36132.1 .
    RefSeqi NP_001003239.1. NM_001003239.1.
    UniGenei Cfa.3778.

    3D structure databases

    ProteinModelPortali Q28923.
    SMRi Q28923. Positions 89-539.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9615.ENSCAFP00000027127.

    Proteomic databases

    PRIDEi Q28923.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 403917.
    KEGGi cfa:403917.

    Organism-specific databases

    CTDi 7525.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233858.
    HOVERGENi HBG008761.
    InParanoidi Q28923.
    KOi K05705.

    Enzyme and pathway databases

    BRENDAi 2.7.10.2. 1154.

    Miscellaneous databases

    NextBioi 20817409.

    Family and domain databases

    Gene3Di 3.30.505.10. 1 hit.
    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR001452. SH3_domain.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view ]
    Pfami PF07714. Pkinase_Tyr. 1 hit.
    PF00017. SH2. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00401. SH2DOMAIN.
    PR00452. SH3DOMAIN.
    PR00109. TYRKINASE.
    SMARTi SM00252. SH2. 1 hit.
    SM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    SSF55550. SSF55550. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50001. SH2. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Spleen.
    2. "Nonreceptor tyrosine kinase c-Yes interacts with occludin during tight junction formation in canine kidney epithelial cells."
      Chen Y.H., Lu Q., Goodenough D.A., Jeansonne B.
      Mol. Biol. Cell 13:1227-1237(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH OCLLUDIN/OCLN, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiYES_CANFA
    AccessioniPrimary (citable) accession number: Q28923
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 119 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3