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Q28923

- YES_CANFA

UniProt

Q28923 - YES_CANFA

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Protein

Tyrosine-protein kinase Yes

Gene
YES1, YES
Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis By similarity.

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei301 – 3011ATP By similarity
Active sitei392 – 3921Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi279 – 2879ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 1154.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase Yes (EC:2.7.10.2)
Alternative name(s):
Proto-oncogene c-Yes
p61-Yes
Gene namesi
Name:YES1
Synonyms:YES
OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
ProteomesiUP000002254: Unplaced

Subcellular locationi

Cell membrane By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cytoplasmcytosol By similarity
Note: Newly synthesized protein initially accumulates in the Golgi region and traffics to the plasma membrane through the exocytic pathway By similarity.1 Publication

GO - Cellular componenti

  1. cytosol Source: UniProtKB-SubCell
  2. microtubule organizing center Source: UniProtKB-SubCell
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 539538Tyrosine-protein kinase YesPRO_0000088180Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine By similarity
Lipidationi3 – 31S-palmitoyl cysteine; in membrane form By similarity
Modified residuei19 – 191Phosphothreonine By similarity
Modified residuei28 – 281Phosphotyrosine By similarity
Modified residuei107 – 1071Phosphoserine By similarity
Modified residuei190 – 1901Phosphotyrosine By similarity
Modified residuei191 – 1911Phosphoserine By similarity
Modified residuei218 – 2181Phosphotyrosine By similarity
Modified residuei219 – 2191Phosphotyrosine By similarity
Modified residuei332 – 3321Phosphotyrosine By similarity
Modified residuei341 – 3411Phosphotyrosine By similarity
Modified residuei422 – 4221Phosphotyrosine; by autocatalysis By similarity
Modified residuei442 – 4421Phosphotyrosine By similarity
Modified residuei533 – 5331Phosphotyrosine; by CSK By similarity

Post-translational modificationi

Phosphorylation by CSK on the C-terminal tail maintains the enzyme in an inactive state. Autophosphorylation at Tyr-422 maintains enzyme activity by blocking CSK-mediated inhibition By similarity.
Palmitoylation at Cys-3 promotes membrane localization By similarity.

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

PRIDEiQ28923.

Interactioni

Subunit structurei

Interacts with YAP1. Interacts with FASLG. Interacts with CTNND1; this interaction allows YES1-mediated activation of FYN and FER and subsequent phosphorylation of CTNND1. Interacts with CSF1R By similarity.1 Publication

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000027127.

Structurei

3D structure databases

ProteinModelPortaliQ28923.
SMRiQ28923. Positions 89-539.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini87 – 14862SH3Add
BLAST
Domaini154 – 25198SH2Add
BLAST
Domaini273 – 526254Protein kinaseAdd
BLAST

Sequence similaritiesi

Contains 1 SH2 domain.
Contains 1 SH3 domain.

Keywords - Domaini

SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233858.
HOVERGENiHBG008761.
InParanoidiQ28923.
KOiK05705.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q28923-1 [UniParc]FASTAAdd to Basket

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MGCIKSKEDK GPAIKYRNTP EPVSVSHYGA EPTQATPWPS SSGWGTAFNF    50
SSLSMTGFGG SSGVTPFGGA SSSFSVVPSP YPAGLTGGVT IFVALYDYEA 100
RTTEDLSFKG GERFQIINNT EGDWWEARSI ATGKNGYIPS NYVAPADSIA 150
AEEWYFGKMG RKDAERLLLN PGNQRGIFLV RESETTKGAY SLSIRDWDEI 200
RGDNVKHYKI RKLDNGGYYI TTRAQFDTLQ KLVKHSTEHA DGLCHKLTTV 250
CPTVKPQTQG LAKDAWEIPR ESLRLEVKLG QGCFGEVWMG TWNGTTKVAI 300
KTLKLGTMMP EAFLQEAQIM KKLRHDKLVP LYAVVSEEPI YIVTEFMSKG 350
SLLDFLKEGD GKYLKLPQLV DMAAQIADGM AYIERMNYIH RDLRAANILV 400
GENLVCKIAD FGLARLIEDN EYTARQGAKF PIKWTAPEAA LYGRFTIKSD 450
VWSFGILQTE LTTKGRVPYP GMVNREVLEQ VERGYRMPCP QGCPESLHEL 500
MNLCWKKDPD ERPTFEYIQS FLEDYFTAAE PQYQPGENL 539
Length:539
Mass (Da):60,300
Last modified:January 23, 2007 - v3
Checksum:iCC4BEA38073CC106
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S81472 mRNA. Translation: AAB36132.1.
RefSeqiNP_001003239.1. NM_001003239.1.
UniGeneiCfa.3778.

Genome annotation databases

GeneIDi403917.
KEGGicfa:403917.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S81472 mRNA. Translation: AAB36132.1 .
RefSeqi NP_001003239.1. NM_001003239.1.
UniGenei Cfa.3778.

3D structure databases

ProteinModelPortali Q28923.
SMRi Q28923. Positions 89-539.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9615.ENSCAFP00000027127.

Proteomic databases

PRIDEi Q28923.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 403917.
KEGGi cfa:403917.

Organism-specific databases

CTDi 7525.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233858.
HOVERGENi HBG008761.
InParanoidi Q28923.
KOi K05705.

Enzyme and pathway databases

BRENDAi 2.7.10.2. 1154.

Miscellaneous databases

NextBioi 20817409.

Family and domain databases

Gene3Di 3.30.505.10. 1 hit.
InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view ]
Pfami PF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view ]
PRINTSi PR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTi SM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Spleen.
  2. "Nonreceptor tyrosine kinase c-Yes interacts with occludin during tight junction formation in canine kidney epithelial cells."
    Chen Y.H., Lu Q., Goodenough D.A., Jeansonne B.
    Mol. Biol. Cell 13:1227-1237(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH OCLLUDIN/OCLN, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiYES_CANFA
AccessioniPrimary (citable) accession number: Q28923
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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