Q28901 (F263_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
Short name=6PF-2-K/Fru-2,6-P2ase 3
Short name=PFK/FBPase 3

Alternative name(s):
6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme

Including the following 2 domains:

1. 6-phosphofructo-2-kinase
   EC=2.7.1.105
2. Fructose-2,6-bisphosphatase
   EC=3.1.3.46

Gene names

Name:

PFKFB3

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	463 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Synthesis and degradation of fructose 2,6-bisphosphate.
Catalytic activity	Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate. ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.
Subunit structure	Homodimer By similarity.
Tissue specificity	Brain.
Post-translational modification	Phosphorylation by AMPK stimulates activity By similarity.
Sequence similarities	In the C-terminal section; belongs to the phosphoglycerate mutase family.
Sequence caution	The sequence AAB34145.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	fructose 2,6-bisphosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructo-2-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW fructose-2,6-bisphosphate 2-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›463	›463	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3		PRO_0000179967
Regions
	Nucleotide binding	42 – 49	8	ATP By similarity
	Region	1 – 246	246	6-phosphofructo-2-kinase
	Region	247 – ›463	›217	Fructose-2,6-bisphosphatase
Sites
	Active site	125	1	Potential
	Active site	155	1	Potential
	Active site	255	1	Tele-phosphohistidine intermediate By similarity
	Active site	324	1	Potential
	Active site	389	1	Proton donor By similarity
	Binding site	99	1	Fructose-6-phosphate By similarity
	Binding site	191	1	Fructose-6-phosphate By similarity
Amino acid modifications
	Modified residue	462	1	Phosphoserine; by AMPK and PKA By similarity
Experimental info
	Non-terminal residue	463	1

Sequences

	Sequence		Length	Mass (Da)
	Q28901 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: F04FA28D1F81F325	FASTA	463	53,584
	10 20 30 40 50 60 MPLELTQSRV QKIWIPVDHR PSLPRTCGPK LTNSPTVIVM VGLPARGKTY ISKKLTRYLN 70 80 90 100 110 120 WIGVPTKVFN LGEYRRDGVK QYSSYNFFRP DNEEAMKVRK QCALAALRDV KSYLTKEGGQ 130 140 150 160 170 180 IAVFDATNTT RERRHMILHF PKENDFKVFF IESVCDDPTV VASNIMEVKI SSPDYKDCNS 190 200 210 220 230 240 RENAMDDFMK RINCYEASYQ PLDPDNDDRD LSLIKVIDVG QRFLVNRVQD HIQRRIVYYL 250 260 270 280 290 300 MNIHWQPRTI YLCRHGESKH NLQGKIGGDS GLSSRGRKFA NALSKFVEEQ NLKDLKVWTS 310 320 330 340 350 360 QLKSTIQTAE ALQLPYEQWK ALNEIDAGVC EEMTYEEIKD TYPEEYALAE ADKYYYRYPT 370 380 390 400 410 420 GESYQDLVQR LEPVIMELER QENVLVICHQ AVCVCLLAYF LDKSAEEMPY LKCPLHAVLK 430 440 450 460 LTPIAYGCRV ESIYLNVESV STHRERSEDA KKGPNPLMRS NSH

References

[1]	"Cloning and expression of a catalytic core bovine brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase." Ventura F., Ambrosio S., Bartrons R., El-Maghrabi M.R., Lange A.J., Pilkis S.J. Biochem. Biophys. Res. Commun. 209:1140-1148(1995) [PubMed: 7733968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S77845 mRNA. Translation: AAB34145.2. Different initiation.
IPI	IPI00708598.
UniGene	Bt.12314.
3D structure databases
ProteinModelPortal	Q28901.
SMR	Q28901. Positions 3-462.
ModBase	Search...
Protein-protein interaction databases
STRING	Q28901.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
GeneTree	ENSGT00390000018751.
HOVERGEN	HBG005628.
InParanoid	Q28901.
OrthoDB	EOG4G4GQ4.
PhylomeDB	Q28901.
Enzyme and pathway databases
BRENDA	3.1.3.46. 908.
Family and domain databases
InterPro	IPR003094. 6Pfruct_kin. IPR013079. 6Phosfructo_kin. IPR016260. Bifunct_6PFK/fruc_bisP_Ptase. IPR013078. His_Pase_superF_clade-1. IPR001345. PG/BPGM_mutase_AS. [Graphical view]
PANTHER	PTHR10606. 6Pfruct_kin. 1 hit.
Pfam	PF01591. 6PF2K. 1 hit. PF00300. His_Phos_1. 1 hit. [Graphical view]
PIRSF	PIRSF000709. 6PFK_2-Ptase. 1 hit.
PRINTS	PR00991. 6PFRUCTKNASE.
SMART	SM00855. PGAM. 1 hit. [Graphical view]
PROSITE	PS00175. PG_MUTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F263_BOVIN

Accession

Primary (citable) accession number: Q28901

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 15, 1998
Last modified:	October 19, 2011
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program