Reviewed, UniProtKB/Swiss-Prot Q28892 (S5A2_MACFA)

Names and origin

Protein names

Recommended name:
    3-oxo-5-alpha-steroid 4-dehydrogenase 2
    EC=1.3.99.5
Alternative name(s):
    Steroid 5-alpha-reductase 2
    5 alpha-SR2
    SR type 2

Gene names

Name:

SRD5A2

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
Catalytic activity	A 3-oxo-5-alpha-steroid + acceptor = a 3-oxo-Delta(4)-steroid + reduced acceptor.
Subcellular location	Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane proteinPotential.
Sequence similarities	Belongs to the steroid 5-alpha reductase family.
Biophysicochemical properties	pH dependence: Optimally active at acidic pHs.

Ontologies

Keywords
Biological process	Differentiation Sexual differentiation
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sex determination Inferred from electronic annotation. Source: UniProtKB-KW steroid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: InterPro microsome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-oxo-5-alpha-steroid 4-dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	3-oxo-5-alpha-steroid 4-dehydrogenase 2		PRO_0000213677
Regions
	Transmembrane	8 – 28	21	Potential
	Transmembrane	72 – 92	21	Potential
	Transmembrane	146 – 166	21	Potential
	Transmembrane	206 – 226	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q28892-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 0699C2E07C77653B	FASTA	254	28,504
	10 20 30 40 50 60 MQVQCQQSPV LAGSATLVAL GALVLYVAKP SGYGKHTESL KPAATRLPAR AAWFLQELPS 70 80 90 100 110 120 FAVPAGILAR QPLSLFGPPG TVLLGLFCVH YFHRTFVYSL LNRGRPYPAV LIFRGIAFCA 130 140 150 160 170 180 GNGFLQSYYL IYCAEYPDGW YTDIRFCLGV FLFILGMGVN IHSDYILRQL RKPGEITYRI 190 200 210 220 230 240 PKGGLFTYVS GANFLGEIIE WIGYALATWS LPALAFAFFS VCFLGLRAFH HHRFYLKMFE 250 DYPKSRKALI PFIF

References

[1]

"Cloning, expression and functional characterization of type 1 and type 2 steroid 5 alpha-reductases from Cynomolgus monkey: comparisons with human and rat isoenzymes." Levy M.A., Brandt M., Sheedy K.M., Holt D.A., Heaslip J.I., Trill J.J., Ryan P.J., Morris R.A., Garrison L.M., Bergsma D.J. J. Steroid Biochem. Mol. Biol. 52:307-319(1995) [PubMed: 7734398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Prostate.

Cross-references

Sequence databases
EMBLGenBankDDBJ	S77165 mRNA. Translation: AAB34213.1.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q28892.
Family and domain databases
InterPro	IPR016636. 3-oxo-5-alpha-steroid_4-DH. IPR001104. 3-oxo-5_a-steroid_4-DHase_C. IPR002110. ANK. [Graphical view]
Pfam	PF02544. Steroid_dh. 1 hit. [Graphical view]
PIRSF	PIRSF015596. 5_alpha-SR2. 1 hit.
PRINTS	PR01415. ANKYRIN.
PROSITE	PS50244. S5A_REDUCTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

S5A2_MACFA

Accession

Primary (citable) accession number: Q28892

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 25, 2008
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)