Q28892 (S5A2_MACFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxo-5-alpha-steroid 4-dehydrogenase 2
EC=1.3.1.22

Alternative name(s):
5 alpha-SR2
SR type 2
Steroid 5-alpha-reductase 2
Short name=S5AR 2

Gene names

Name:

SRD5A2

Organism

Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Cercopithecoidea › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
Catalytic activity	A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.
Subcellular location	Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the steroid 5-alpha reductase family.
Biophysicochemical properties	pH dependence: Optimally active at acidic pHs.

Ontologies

Keywords
Biological process	Differentiation Sexual differentiation
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane Transmembrane helix
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW sex differentiation Inferred from electronic annotation. Source: UniProtKB-KW steroid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	3-oxo-5-alpha-steroid 4-dehydrogenase activity Inferred from electronic annotation. Source: InterPro cholestenone 5-alpha-reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	3-oxo-5-alpha-steroid 4-dehydrogenase 2		PRO_0000213677
Regions
	Transmembrane	8 – 28	21	Helical; Potential
	Transmembrane	72 – 92	21	Helical; Potential
	Transmembrane	146 – 166	21	Helical; Potential
	Transmembrane	206 – 226	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q28892 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 0699C2E07C77653B	FASTA	254	28,504
	10 20 30 40 50 60 MQVQCQQSPV LAGSATLVAL GALVLYVAKP SGYGKHTESL KPAATRLPAR AAWFLQELPS 70 80 90 100 110 120 FAVPAGILAR QPLSLFGPPG TVLLGLFCVH YFHRTFVYSL LNRGRPYPAV LIFRGIAFCA 130 140 150 160 170 180 GNGFLQSYYL IYCAEYPDGW YTDIRFCLGV FLFILGMGVN IHSDYILRQL RKPGEITYRI 190 200 210 220 230 240 PKGGLFTYVS GANFLGEIIE WIGYALATWS LPALAFAFFS VCFLGLRAFH HHRFYLKMFE 250 DYPKSRKALI PFIF

References

[1]

"Cloning, expression and functional characterization of type 1 and type 2 steroid 5 alpha-reductases from Cynomolgus monkey: comparisons with human and rat isoenzymes." Levy M.A., Brandt M., Sheedy K.M., Holt D.A., Heaslip J.I., Trill J.J., Ryan P.J., Morris R.A., Garrison L.M., Bergsma D.J. J. Steroid Biochem. Mol. Biol. 52:307-319(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Prostate.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S77165 mRNA. Translation: AAB34213.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG003402.
Family and domain databases
InterPro	IPR016636. 3-oxo-5-alpha-steroid_4-DH. IPR001104. 3-oxo-5_a-steroid_4-DH_C. [Graphical view]
Pfam	PF02544. Steroid_dh. 1 hit. [Graphical view]
PIRSF	PIRSF015596. 5_alpha-SR2. 1 hit.
PROSITE	PS50244. S5A_REDUCTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

S5A2_MACFA

Accession

Primary (citable) accession number: Q28892

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program