Reviewed, UniProtKB/Swiss-Prot Q28891 (S5A1_MACFA)

Names and origin

Protein names

Recommended name:
    3-oxo-5-alpha-steroid 4-dehydrogenase 1
    EC=1.3.99.5
Alternative name(s):
    Steroid 5-alpha-reductase 1
    SR type 1

Gene names

Name:

SRD5A1

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
Catalytic activity	A 3-oxo-5-alpha-steroid + acceptor = a 3-oxo-Delta(4)-steroid + reduced acceptor.
Subcellular location	Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the steroid 5-alpha reductase family.
Biophysicochemical properties	pH dependence: Optimally active at alkaline pHs.

Ontologies

Keywords
Biological process	Differentiation Sexual differentiation
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sex differentiation Inferred from electronic annotation. Source: UniProtKB-KW steroid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-oxo-5-alpha-steroid 4-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	3-oxo-5-alpha-steroid 4-dehydrogenase 1		PRO_0000213675
Regions
	Transmembrane	16 – 33	18	Potential
	Transmembrane	90 – 110	21	Potential
	Transmembrane	115 – 135	21	Potential
	Transmembrane	155 – 175	21	Potential
	Transmembrane	213 – 233	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q28891-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 8830E1FB8F7365C4	FASTA	263	30,018
	10 20 30 40 50 60 MATAVAEELL LAEERMLAAL AYLQCAVGCA VLARNRETNL AYGRHASPSF RVRVPARAAW 70 80 90 100 110 120 VVQELPSLAL PLYQYASESA PRLRSAPNCI LLAMFLVHYG HRCLIYPFLM RGGKPMPLLA 130 140 150 160 170 180 CTMAIMFCTF NGYLQSRYLS HWAVYADDWV TDPRFLIGFG LWLAGMLINI HSDHILRNLR 190 200 210 220 230 240 KPGDTGYKIP RGGLFEYVTA ANYFGEIMEW CGYALASWSV QGAAFAFFTF CFLSGRAKEH 250 260 HRWYLQKFEE YPKFRKILIP FLF

References

[1]

"Cloning, expression and functional characterization of type 1 and type 2 steroid 5 alpha-reductases from Cynomolgus monkey: comparisons with human and rat isoenzymes." Levy M.A., Brandt M., Sheedy K.M., Holt D.A., Heaslip J.I., Trill J.J., Ryan P.J., Morris R.A., Garrison L.M., Bergsma D.J. J. Steroid Biochem. Mol. Biol. 52:307-319(1995) [PubMed: 7734398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Prostate.

Cross-references

Sequence databases
EMBLGenBankDDBJ	S77162 mRNA. Translation: AAB34212.1.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q28891.
Enzyme and pathway databases
BRENDA	1.3.99.5. 3438.
Family and domain databases
InterPro	IPR016636. 3-oxo-5-alpha-steroid_4-DH. IPR001104. 3-oxo-5_a-steroid_4-DH_C. [Graphical view]
Pfam	PF02544. Steroid_dh. 1 hit. [Graphical view]
PIRSF	PIRSF015596. 5_alpha-SR2. 1 hit.
PROSITE	PS50244. S5A_REDUCTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

S5A1_MACFA

Accession

Primary (citable) accession number: Q28891

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	October 13, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)