Q28811 (PHR_POTTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyribodipyrimidine photo-lyase
EC=4.1.99.3

Alternative name(s):
DNA photolyase
Photoreactivating enzyme

Gene names

Name:

PHR

Organism

Potorous tridactylus (Potoroo)

Taxonomic identifier

9310 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Metatheria › Diprotodontia › Potoroidae › Potorous

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation.
Catalytic activity	Cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA).
Cofactor	FAD.
Sequence similarities	Belongs to the DNA photolyase class-2 family. Contains 1 photolyase/cryptochrome alpha/beta domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Ligand	Chromophore DNA-binding FAD Flavoprotein
Molecular function	Lyase
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW deoxyribodipyrimidine photo-lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 532	532	Deoxyribodipyrimidine photo-lyase		PRO_0000085120
Regions
	Domain	97 – 229	133	Photolyase/cryptochrome alpha/beta
	Nucleotide binding	468 – 470	3	FAD By similarity
	Region	368 – 376	9	Interaction with DNA By similarity
	Region	442 – 443	2	Interaction with DNA By similarity
Sites
	Binding site	322	1	DNA By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q28811 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 0865B1BFDE7BB25B	FASTA	532	61,631
	10 20 30 40 50 60 MDSKKRSHST GGEAENMESQ ESKAKRKPLQ KHQFSKSNVV QKEEKDKTEG EEKGAEGLQE 70 80 90 100 110 120 VVRQSRLRTA PSVLEFRFNK QRVRLISQDC HLQDQSQAFV YWMSRDQRVQ DNWAFLYAQR 130 140 150 160 170 180 LALKQKLPLH VCFCLAPCFL GATIRHYDFM LRGLEEVAEE CEKLCIPFHL LLGLPKDVLP 190 200 210 220 230 240 AFVQTHGIGG IVTDFSPLLH HTQWVKDVQD ALPRQVPFVQ VDAHNIVPCW VASDKQEYGA 250 260 270 280 290 300 RTIRHKIHDR LPHFLTEFPP VICHPYTSNV QAEPVDWNGC RAGLQVDRSV KEVSWAKPGT 310 320 330 340 350 360 ASGLTMLQSF IAERLPYFGS DRNNPNKDAL SNLSPWFHFG QVSVQRAILE VQKHRSRYPD 370 380 390 400 410 420 SVTNFVEEAV VRRELADNFC FYNKNYDKLE GAYDWAQTTL RLHAKDKRPH LYSLEQLESG 430 440 450 460 470 480 KTHDPLWNAA QMQTVKEGKM HGFLRMYWAK KILEWTRSPE EALEFAIYLN DRFQLDGWDP 490 500 510 520 530 NGYVGCMWSI CGIHDQGWAE REIFGKIRYM NYAGCKRKFD VAEFERKISP AD

References

[1]	"A new class of DNA photolyases present in various organisms including aplacental mammals." Yasui A., Eker A.P., Yasuhira S., Yajima H., Kobayashi T., Takao M., Oikawa A. EMBO J. 13:6143-6151(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	D26020 mRNA. Translation: BAA05041.1.
PIR	S52046.
3D structure databases
ProteinModelPortal	Q28811.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG008186.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
InterPro	IPR008148. DNA_photolyase_2. IPR006050. DNA_photolyase_N. IPR005101. Photolyase_FAD-bd/Cryptochr_C. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10211. PTHR10211. 1 hit.
Pfam	PF00875. DNA_photolyase. 1 hit. PF03441. FAD_binding_7. 1 hit. [Graphical view]
SUPFAM	SSF52425. DNA_photolyase_N. 1 hit. SSF48173. Photolyase_FAD-bd/Cryptochr_C. 1 hit.
TIGRFAMs	TIGR00591. phr2. 1 hit.
PROSITE	PS01083. DNA_PHOTOLYASES_2_1. 1 hit. PS01084. DNA_PHOTOLYASES_2_2. 1 hit. PS51645. PHR_CRY_ALPHA_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHR_POTTR

Accession

Primary (citable) accession number: Q28811

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program