Reviewed, UniProtKB/Swiss-Prot Q28773 (KLK1_PAPHA)

Names and origin

Protein names

Recommended name:
    Kallikrein-1
    EC=3.4.21.35
Alternative name(s):
    Tissue kallikrein
    Kidney/pancreas/salivary gland kallikrein

Gene names

Name:

KLK1

Organism

Papio hamadryas (Hamadryas baboon)

Taxonomic identifier

9557 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Papio

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Catalytic activity	Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	By similarity
	Propeptide	19 – 24	6	Activation peptide Probable		PRO_0000027927
	Chain	25 – 258	234	Kallikrein-1		PRO_0000027928
Regions
	Domain	25 – 255	231	Peptidase S1
Sites
	Active site	62	1	Charge relay system By similarity
	Active site	117	1	Charge relay system By similarity
	Active site	210	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	90	1	O-linked (GalNAc...) By similarity
	Glycosylation	99	1	N-linked (GlcNAc...) Potential
	Glycosylation	101	1	O-linked (GalNAc...) By similarity
	Glycosylation	105	1	N-linked (GlcNAc...) Potential
	Glycosylation	161	1	N-linked (GlcNAc...) Potential
	Glycosylation	163	1	O-linked (GalNAc...) By similarity
	Disulfide bond	31 ↔ 170		By similarity
	Disulfide bond	47 ↔ 63		By similarity
	Disulfide bond	149 ↔ 216		By similarity
	Disulfide bond	181 ↔ 195		By similarity
	Disulfide bond	206 ↔ 231		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q28773-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 8254BFEBC84F36E4	FASTA	258	28,339
	10 20 30 40 50 60 MWFLVLCLAL SLGGTGAAPP IQSRIVGGWE CSQPWQAALY HFSTFQCGGI LVHPQWVLTA 70 80 90 100 110 120 AHCIGDNYQL WLGRHNLFDD EDTAQFVHVS ESFPHPCFNM SLLKNHTRQA DEDYSHDLML 130 140 150 160 170 180 LRLTQPAEIT DAVQVVELPT QEPEVGSTCL ASGWGSIEPE NFSYPDDLQC VDLKILPNDK 190 200 210 220 230 240 CAKAHTQKVT EFMLCAGHLE GGKDTCVGDS GGPLTCDGVL QGVTSWGYIP CGSPNKPAVF 250 VRVLSYVKWI EDTIAENS

References

[1]	"Characterization of the baboon glandular kallikrein locus." Perelygina L.M., Kammerer C.M., Henkel R.D. Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pancreas.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L43121 mRNA. Translation: AAA73523.1.
3D structure databases
HSSP	HSSP built from PDB template 1FDP based on UniProtKB P00746.
SMR	Q28773. Positions 25-258.
ModBase	Search...
Protein family/group databases
MEROPS	S01.160.
Phylogenomic databases
HOVERGEN	Q28773.
Enzyme and pathway databases
BRENDA	3.4.21.35. 39388.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KLK1_PAPHA

Accession

Primary (citable) accession number: Q28773

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	March 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)