Q28719 (PTGR1_RABIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prostaglandin reductase 1
Short name=PRG-1
EC=1.3.1.-

Alternative name(s):
15-oxoprostaglandin 13-reductase
EC=1.3.1.48
ADRAB-F
NADP-dependent leukotriene B4 12-hydroxydehydrogenase
EC=1.3.1.74

Gene names

Name:	PTGR1
Synonyms:	LTB4DH

Organism

Oryctolagus cuniculus (Rabbit) [Complete proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-oxo-PGE1, 15-oxo-PGE2 and 15-oxo-PGE2-alpha. Has no activity towards PGE1, PGE2 and PGE2-alpha. Catalyzes the conversion of leukotriene B4 into its biologically less active metabolite, 12-oxo-leukotriene B4. This is an initial and key step of metabolic inactivation of leukotriene B4 By similarity.
Catalytic activity	n-alkanal + NAD(P)+ = alk-2-enal + NAD(P)H. 11-alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+ = (5Z)-(13E)-11-alpha-hydroxy-9,15-dioxoprosta-5,13-dienoate + NAD(P)H.
Subunit structure	Monomer or homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NADP-dependent oxidoreductase L4BD family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	15-oxoprostaglandin 13-oxidase activity Inferred from electronic annotation. Source: EC 2-alkenal reductase [NAD(P)] activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	Prostaglandin reductase 1		PRO_0000218068
Regions
	Nucleotide binding	152 – 155	4	NADP By similarity
	Nucleotide binding	239 – 245	7	NADP By similarity
	Nucleotide binding	270 – 272	3	NADP By similarity
Sites
	Binding site	178	1	NADP By similarity
	Binding site	193	1	NADP By similarity
	Binding site	217	1	NADP By similarity
	Binding site	321	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q28719 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 0CABB6EEB027EC16	FASTA	349	38,219
	10 20 30 40 50 60 MVRAKNWTLK KHFHGHPTDS DFELKTVELP PLNNGEVLLE ALFLSVDPYM RLGSKRLKEG 70 80 90 100 110 120 DTMMGQQVAR VVESKNPAWP VGTLVLAHSG WASHSISDGQ QLEKLLTEWP DTLPLSLALG 130 140 150 160 170 180 TVGMPGITAY FGLLEICGAK SGDTVLVNAA AGAVGAVVGQ IAKIKGCRVV GAAGSEEKVD 190 200 210 220 230 240 YLKKIGFDFA FNYKTVKSLE ETLKKAAPDG YDCYFDNVGG EFSNTVIRQM KKFGRVAICG 250 260 270 280 290 300 AISMYNSTGQ LPPGPSPESV LYQEIRMEGF IFNRWKGEVG QKALKELLTW VLEGKIQYRE 310 320 330 340 FVIEGFENMP AAFMRMLKGE NVGKARSESL KSGTCKPGDH PHDLIFPIT

References

[1]	"'Adults only' mRNAs: differential cloning to search for proteins present in intestine of adult but not baby rabbits." Boll W., Mantei N. Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: New Zealand white. Tissue: Small intestine.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z34285 mRNA. Translation: CAA84039.1.
PIR	S47093.
RefSeq	NP_001076171.1. NM_001082702.1.
UniGene	Ocu.2102.
3D structure databases
ProteinModelPortal	Q28719.
SMR	Q28719. Positions 1-325.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100009440.
Organism-specific databases
CTD	22949.
Phylogenomic databases
eggNOG	maNOG06803.
GeneTree	ENSGT00390000009335.
HOVERGEN	HBG055024.
OrthoDB	EOG4N5VXH.
Family and domain databases
InterPro	IPR013149. ADH_C. IPR002085. ADH_SF_Zn-type. IPR014190. B4_12hDH. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF00107. ADH_zinc_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 2 hits.
TIGRFAMs	TIGR02825. B4_12hDH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PTGR1_RABIT

Accession

Primary (citable) accession number: Q28719

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	December 14, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program