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Protein

Dystroglycan

Gene

DAG1

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The dystroglycan complex is involved in a number of processes including laminin and basement membrane assembly, sacrolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization.By similarity
Alpha-dystroglycan is an extracellular peripheral glycoprotein that acts as a receptor for both extracellular matrix proteins containing laminin-G domains. Receptor for laminin-2 (LAMA2) and agrin in peripheral nerve Schwann cells.By similarity
Beta-dystroglycan is a transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton. Acts as a cell adhesion receptor in both muscle and non-muscle tissues. Receptor for both DMD and UTRN and, through these interactions, scaffolds axin to the cytoskeleton. Also functions in cell adhesion-mediated signaling and implicated in cell polarity (By similarity).By similarity

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • laminin-1 binding Source: UniProtKB
  • structural constituent of muscle Source: Ensembl

GO - Biological processi

Complete GO annotation...

Protein family/group databases

MEROPSiS72.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Dystroglycan
Alternative name(s):
Dystrophin-associated glycoprotein 1
Cleaved into the following 2 chains:
Alpha-dystroglycan
Short name:
Alpha-DG
Beta-dystroglycan
Short name:
Beta-DG
Gene namesi
Name:DAG1
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Chromosome 9

Subcellular locationi

Beta-dystroglycan :
  • Cell membrane By similarity; Single-pass type I membrane protein
  • Cytoplasmcytoskeleton
  • Nucleusnucleoplasm
  • Cell membranesarcolemma By similarity
  • Cell junctionsynapsepostsynaptic cell membrane By similarity

  • Note: The monomeric form translocates to the nucleus via the action of importins and depends on RAN. Nuclear transport is inhibited by Tyr-892 phosphorylation. In skeletal muscle, this phosphorylated form locates to a vesicular internal membrane compartment. In muscle cells, sarcolemma localization requires the presence of ANK2, while localization to costameres requires the presence of ANK3 (By similarity). Localizes to neuromuscular junctions (NMJs) in the presence of ANK2 (By similarity). Colocalizes with ERM proteins in Schwann-cell microvilli (By similarity). In peripheral nerves, localizes to the Schwann cell membrane.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini654 – 749ExtracellularAdd BLAST96
Transmembranei750 – 775HelicalSequence analysisAdd BLAST26
Topological domaini776 – 895CytoplasmicSequence analysisAdd BLAST120

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Nucleus, Postsynaptic cell membrane, Secreted, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000002106930 – 653Alpha-dystroglycanAdd BLAST624
ChainiPRO_0000021070654 – 895Beta-dystroglycanAdd BLAST242

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi141N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi182 ↔ 264By similarity
Glycosylationi317O-linked (Man6P...)By similarity1
Glycosylationi319O-linked (Man6P...)By similarity1
Glycosylationi379O-linked (Man6P...)By similarity1
Glycosylationi641N-linked (GlcNAc...)Sequence analysis1
Glycosylationi649N-linked (GlcNAc...)Sequence analysis1
Glycosylationi661N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi669 ↔ 713By similarity
Modified residuei790PhosphothreonineBy similarity1
Modified residuei892Phosphotyrosine; by SRCBy similarity1

Post-translational modificationi

O- and N-glycosylated (By similarity). Alpha-dystroglycan is heavily O-glycosylated comprising of up to two thirds of its mass and the carbohydrate composition differs depending on tissue type. Mucin-type O-glycosylation is important for ligand binding activity. O-mannosylation of alpha-DAG1 is found in high abundance in both brain and muscle where the most abundant glycan is Sia-alpha-2-3-Gal-beta-1-4-Glc-NAc-beta-1-2-Man. O-glycosylated in the N-terminal region with a core 1 or possibly core 8 glycan. The beta subunit is N-glycosylated (By similarity). In muscle, glycosylation on Thr-317, Thr-319 and Thr-379 by a phosphorylated O-mannosyl glycan with the structure 2-(N-acetylamido)-2-deoxygalactosyl-beta-1,3-2-(N-acetylamido)-2-deoxyglucosyl-beta-1,4-6-phosphomannose is mediated by like-acetylglucosaminyltransferase (LARGE1) protein amd is required for laminin binding.By similarity
Autolytic cleavage produces the alpha and beta subunits. In cutaneous cells, as well as in certain pathological conditions, shedding of beta-dystroglcan can occur releasing a peptide of about 30 kDa (By similarity).By similarity
SRC-mediated phosphorylation of the PPXY motif of the beta subunit recruits SH2 domain-containing proteins, but inhibits binding to WWW domain-containing proteins, DMD and UTRN. This phosphorylation also inhibits nuclear entry (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei653 – 654Cleavage; by autolysisBy similarity2
Sitei715 – 716Cleavage; by MMP9By similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiQ28685.

PTM databases

UniCarbKBiQ28685.

Expressioni

Tissue specificityi

Expressed in skeletal muscle, cardiac muscle, lung, brain, liver, kidney, diaphragm and stomach.1 Publication

Interactioni

Subunit structurei

Monomer (By similarity). Heterodimer of alpha- and beta-dystroglycan subunits which are the central components of the dystrophin-glycoprotein complex. This complex then can form a dystrophin-associated glycoprotein complex (DGC) which is composed of three subcomplexes: a cytoplasmic complex comprised of DMD (or UTRN), DTNA and a number of syntrophins, such as SNTB1, SNTB2, SNTG1 and SNTG2, the transmembrane dystroglycan complex, and the sarcoglycan-sarcospan complex. Interacts (via the N-terminal of alphaDAG1) with LARGE1; the interaction enhances laminin binding (PubMed:15210115). Interacts with AGR2 and AGR3. Interacts (betaDAG1) with DMD; the interaction is inhibited by phosphorylation on the PPXY motif. Interacts (betaDAG1, via its PPXY motif) with UTRN (via its WWW and ZZ domains); the interaction is inhibited by phosphorylation on the PPXY motif. Interacts (betaDAG1, via its phosphorylated PPXY motif) with the SH2 domain-containing proteins, FYN, CSK, NCK and SHC. Interacts (betaDAG1) with CAV3 (via a central WW-like domain); the interaction disrupts the binding of DMD (By similarity). Interacts with SGCD. BetaDAG1 directly interacts with ANK3, but not with ANK2; this interaction does not interfere with DMD-binding and is required for retention at costameres. Identified in a dystroglycan complex that contains at least PRX, DRP2, UTRN, DMD and DAG1 (By similarity).By similarity1 Publication

GO - Molecular functioni

  • laminin-1 binding Source: UniProtKB

Protein-protein interaction databases

DIPiDIP-679N.
IntActiQ28685. 2 interactors.
MINTiMINT-1534332.
STRINGi9986.ENSOCUP00000002981.

Structurei

3D structure databases

ProteinModelPortaliQ28685.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini603 – 712Peptidase S72Add BLAST110

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni30 – 408Required for laminin recognitionAdd BLAST379
Regioni49 – 71O-glycosylated at one siteBy similarityAdd BLAST23
Regioni316 – 485Mucin-like domainBy similarityAdd BLAST170
Regioni463 – 485O-glycosylated at seven sites with GalNAcBy similarityAdd BLAST23
Regioni819 – 895Required for interaction with CAV3By similarityAdd BLAST77
Regioni880 – 895Required for binding DMD and UTRNBy similarityAdd BLAST16

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi776 – 782Nuclear localization signalBy similarity7
Motifi889 – 892PPXY motifBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi809 – 895Pro-richAdd BLAST87

Sequence similaritiesi

Contains 1 peptidase S72 domain.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3781. Eukaryota.
ENOG410XQTU. LUCA.
GeneTreeiENSGT00390000008429.
HOGENOMiHOG000072580.
HOVERGENiHBG000078.
InParanoidiQ28685.
KOiK06265.
OMAiAMICYRK.
OrthoDBiEOG091G05R9.
TreeFamiTF328370.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
3.30.70.1040. 1 hit.
InterProiIPR027468. Alpha-dystroglycan_domain_2.
IPR006644. Cadg.
IPR015919. Cadherin-like.
IPR008465. DAG1.
IPR013783. Ig-like_fold.
IPR030398. SEA_DG_dom.
[Graphical view]
PfamiPF05454. DAG1. 1 hit.
[Graphical view]
SMARTiSM00736. CADG. 2 hits.
[Graphical view]
SUPFAMiSSF111006. SSF111006. 1 hit.
SSF49313. SSF49313. 2 hits.
PROSITEiPS51699. SEA_DG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q28685-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRMSVGLSLL LPLWGRTFLL LLCVAVAQSH WPSEPSEAVR DWENQLEASM
60 70 80 90 100
HSVLSDLHEA LPTVVGIPDG TAVVGRSFRV TIPTDLIGSS GEVIKVSTAG
110 120 130 140 150
KEVLPSWLHW DPQSHTLEGL PLDTDKGVHY ISVSAAQLDA NGSHIPQTSS
160 170 180 190 200
VFSIEVYPED HSEPQSVRAA SPDLGEAAAS ACAAEEPVTV LTVILDADLT
210 220 230 240 250
KMTPKQRIDL LHRMQSFSEV ELHNMKLVPV VNNRLFDMSA FMAGPGNAKK
260 270 280 290 300
VVENGALLSW KLGCSLNQNS VPDIRGVEAP AREGTMSAQL GYPVVGWHIA
310 320 330 340 350
NKKPPLPKRI RRQIHATPTP VTAIGPPTTA IQEPPSRIVP TPTSPAIAPP
360 370 380 390 400
TETMAPPVRD PVPGKPTVTT RTRGAIIQTP TLGPIQPTRV SDAGTVVSGQ
410 420 430 440 450
IRATVTIPGY VEPTAVATPP TTTTKKPRVS TPKPATPSTD SSATTTRRPT
460 470 480 490 500
KKPRTPRPVP RVTTKAPITR LETASPPTRI RTTTSGVPRG GEPNQRPELK
510 520 530 540 550
NHIDRVDAWV GTYFEVKIPS DTFYDKEDTT TDKLKLTLKL REQQLVGEKS
560 570 580 590 600
WVQFNSNSQL MYGLPDSSHV GKHEYFMHAT DKGGLSAVDA FEIHVHKRPQ
610 620 630 640 650
GDKAPARFKA KFVGDPAPVV NDIHKKIALV KKLAFAFGDR NCSTVTLQNI
660 670 680 690 700
TRGSIVVEWT NNTLPLEPCP KEQITGLSRR IAEDNGQPRP AFTNALEPDF
710 720 730 740 750
KATSIAITGS GSCRHLQFIP VAPPGIPSSV TPPTEVPDRD PEKSSEDDVY
760 770 780 790 800
LHTVIPAVVV AAILLIAGII AMICYRKKRK GKLTLEDQAT FIKKGVPIIF
810 820 830 840 850
ADELDDSKPP PSSSMPLILQ EEKAPLPPPE YPSQSVPETT PLNQDTVGEY
860 870 880 890
TPLRDEDPNA PPYQPPPPFT APMEGKGSRP KNMTPYRSPP PYVPP
Length:895
Mass (Da):97,030
Last modified:November 1, 1996 - v1
Checksum:iC2B9E4733A0AB82A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64393 mRNA. Translation: CAA45732.1.
PIRiPN0662.
PN0663.
S20582.
RefSeqiNP_001095170.1. NM_001101700.1.
XP_017199133.1. XM_017343644.1.
XP_017199134.1. XM_017343645.1.
UniGeneiOcu.2077.

Genome annotation databases

EnsembliENSOCUT00000003437; ENSOCUP00000002981; ENSOCUG00000003439.
GeneIDi100009278.
KEGGiocu:100009278.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64393 mRNA. Translation: CAA45732.1.
PIRiPN0662.
PN0663.
S20582.
RefSeqiNP_001095170.1. NM_001101700.1.
XP_017199133.1. XM_017343644.1.
XP_017199134.1. XM_017343645.1.
UniGeneiOcu.2077.

3D structure databases

ProteinModelPortaliQ28685.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-679N.
IntActiQ28685. 2 interactors.
MINTiMINT-1534332.
STRINGi9986.ENSOCUP00000002981.

Protein family/group databases

MEROPSiS72.001.

PTM databases

UniCarbKBiQ28685.

Proteomic databases

PRIDEiQ28685.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSOCUT00000003437; ENSOCUP00000002981; ENSOCUG00000003439.
GeneIDi100009278.
KEGGiocu:100009278.

Organism-specific databases

CTDi1605.

Phylogenomic databases

eggNOGiKOG3781. Eukaryota.
ENOG410XQTU. LUCA.
GeneTreeiENSGT00390000008429.
HOGENOMiHOG000072580.
HOVERGENiHBG000078.
InParanoidiQ28685.
KOiK06265.
OMAiAMICYRK.
OrthoDBiEOG091G05R9.
TreeFamiTF328370.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
3.30.70.1040. 1 hit.
InterProiIPR027468. Alpha-dystroglycan_domain_2.
IPR006644. Cadg.
IPR015919. Cadherin-like.
IPR008465. DAG1.
IPR013783. Ig-like_fold.
IPR030398. SEA_DG_dom.
[Graphical view]
PfamiPF05454. DAG1. 1 hit.
[Graphical view]
SMARTiSM00736. CADG. 2 hits.
[Graphical view]
SUPFAMiSSF111006. SSF111006. 1 hit.
SSF49313. SSF49313. 2 hits.
PROSITEiPS51699. SEA_DG. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDAG1_RABIT
AccessioniPrimary (citable) accession number: Q28685
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.