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Protein

Solute carrier family 12 member 4

Gene

SLC12A4

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. May be involved in the regulation of basolateral Cl- exit in NaCl absorbing epithelia.

Enzyme regulationi

Inhibited by WNK3.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ion transport, Potassium transport, Symport, Transport

Keywords - Ligandi

Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 12 member 4
Alternative name(s):
Electroneutral potassium-chloride cotransporter 1
Erythroid K-Cl cotransporter 1
rbKCC1
Gene namesi
Name:SLC12A4
Synonyms:KCC1
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 118118CytoplasmicSequence analysisAdd
BLAST
Transmembranei119 – 13921HelicalSequence analysisAdd
BLAST
Transmembranei149 – 16921HelicalSequence analysisAdd
BLAST
Topological domaini170 – 21445CytoplasmicSequence analysisAdd
BLAST
Transmembranei215 – 23521HelicalSequence analysisAdd
BLAST
Transmembranei253 – 27321HelicalSequence analysisAdd
BLAST
Topological domaini274 – 2752CytoplasmicSequence analysis
Transmembranei276 – 29621HelicalSequence analysisAdd
BLAST
Transmembranei356 – 37621HelicalSequence analysisAdd
BLAST
Topological domaini377 – 40731CytoplasmicSequence analysisAdd
BLAST
Transmembranei408 – 42821HelicalSequence analysisAdd
BLAST
Transmembranei453 – 47321HelicalSequence analysisAdd
BLAST
Topological domaini474 – 49320CytoplasmicSequence analysisAdd
BLAST
Transmembranei494 – 51421HelicalSequence analysisAdd
BLAST
Transmembranei567 – 58721HelicalSequence analysisAdd
BLAST
Topological domaini588 – 62740CytoplasmicSequence analysisAdd
BLAST
Transmembranei628 – 64821HelicalSequence analysisAdd
BLAST
Transmembranei845 – 86521HelicalSequence analysisAdd
BLAST
Topological domaini866 – 1085220CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10851085Solute carrier family 12 member 4PRO_0000178032Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei24 – 241PhosphoserineBy similarity
Modified residuei47 – 471PhosphoserineBy similarity
Modified residuei59 – 591PhosphoserineBy similarity
Modified residuei88 – 881PhosphoserineBy similarity
Glycosylationi245 – 2451N-linked (GlcNAc...)Sequence analysis
Glycosylationi312 – 3121N-linked (GlcNAc...)Sequence analysis
Glycosylationi331 – 3311N-linked (GlcNAc...)Sequence analysis
Glycosylationi347 – 3471N-linked (GlcNAc...)Sequence analysis
Glycosylationi439 – 4391N-linked (GlcNAc...)Sequence analysis
Modified residuei967 – 9671PhosphoserineBy similarity

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Interactioni

Subunit structurei

Homomultimer and heteromultimer with other K-Cl cotransporters.By similarity

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000005495.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi160 – 1634Poly-Cys

Sequence similaritiesi

Belongs to the SLC12A transporter family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2082. Eukaryota.
COG0531. LUCA.
HOGENOMiHOG000092644.
HOVERGENiHBG052852.
InParanoidiQ28677.
KOiK14427.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR000622. KCC1.
IPR000076. KCL_cotranspt.
IPR018491. SLC12_C.
IPR004842. SLC12A_fam.
[Graphical view]
PANTHERiPTHR11827:SF46. PTHR11827:SF46. 4 hits.
PfamiPF00324. AA_permease. 2 hits.
PF03522. SLC12. 2 hits.
[Graphical view]
PRINTSiPR01081. KCLTRNSPORT.
PR01082. KCLTRNSPORT1.
TIGRFAMsiTIGR00930. 2a30. 1 hit.

Sequencei

Sequence statusi: Complete.

Q28677-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPHFTVVPVD GPRRGDYDNL EGLSWVDYGE RAEREDPDGH GNHRESSPFL
60 70 80 90 100
CPLEASRGSD YYDRNLALFE EELDIRPKVS SLLGKLVSYT NLTQGAKEHE
110 120 130 140 150
EAESGEGTRR RAAKAPSMGT LMGVYLPCLQ NIFGVILFLR LTWMVGTAGV
160 170 180 190 200
LQALLIVLIC CCCTLLTAIS MSAIATNGVV PAGGSYFMIS RSLGPEFGGA
210 220 230 240 250
VGLCFYLGTT FAAAMYILGA IEILLTYIAP PAAIFYPSGT HDTSNATLNN
260 270 280 290 300
MRVYGTVFLS FMTLVVFVGV KYVNKFASLF LACVIISILS IYAGGIKSMF
310 320 330 340 350
DPPVFPVCML GNRTLSRDQF DICAKTTMVD NETVATRLWS FFCHSPNLTT
360 370 380 390 400
DSCDPYFLLN NVTEIPGIPG AAAGVLQENL WSAYLEKGEV VEKRGLPSTD
410 420 430 440 450
AVGLKENLPL YVVADIATSF TVLVGIFFPS VTGIMAGSNR SGDLRDAQKS
460 470 480 490 500
IPVGTILAIV TTSLVYFSSV VLFGACIEGV VLRDKYGDGV SRNLVVGTLA
510 520 530 540 550
WPSPWVIVVG SFFSTCGAGL QSLTGAPRLL QAIAKDNIIP FLRVFGHGKA
560 570 580 590 600
NGEPTWALLL TALIAELGIL IASLDMVAPI LSMFFLMCYL FVNLACAVQT
610 620 630 640 650
LLRTPNWRPR FKYYHWALSF LGMSLCLALM FVSSWYYALV AMLIAGMIYK
660 670 680 690 700
YIEYQGAEKE WGDGIRGLSL SAARYALLRL EEGPPHTKNW RPQLLVLLKL
710 720 730 740 750
DEDLHVKYPR LLTFASQLKA GKGLTIVGSV IQGSFLESYG EAQAAEQTIK
760 770 780 790 800
NMMKIEKVKG FCQVVVASKV REGLAHLIQS CGLGGMRHNS VVLGWPYGWR
810 820 830 840 850
QSEDPRAWKT FIDTVRCTTA AHLALLVPKN IAFYPSNHER YLEGHIDVWW
860 870 880 890 900
IVHDGGMLML LPFLLRQHKV WRKCRMRIFT VAQMDDNSIQ MKKDLAVFLY
910 920 930 940 950
HLRLEAEVEV VEMHNSDISA YTYERTLMME QRSQMLRQMR LTKTEREREA
960 970 980 990 1000
QLVKDRHSAL RLESLYSDEE DEAAAGADKI QMTWTRDKYM TEPWDPSHTP
1010 1020 1030 1040 1050
DNFRELVHIK PDQSNVRRMH TAVKLNEVIV TRSHDARLVL LNMPGPPKNS
1060 1070 1080
EGDENYMEFL EVLTEGLERV LLVRGGGREV ITIYS
Length:1,085
Mass (Da):120,743
Last modified:November 1, 1996 - v1
Checksum:i717408738E226B8E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U55053 mRNA. Translation: AAC48593.1.
PIRiT18369.
RefSeqiNP_001076172.1. NM_001082703.2.
UniGeneiOcu.2291.

Genome annotation databases

GeneIDi100009441.
KEGGiocu:100009441.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U55053 mRNA. Translation: AAC48593.1.
PIRiT18369.
RefSeqiNP_001076172.1. NM_001082703.2.
UniGeneiOcu.2291.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000005495.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100009441.
KEGGiocu:100009441.

Organism-specific databases

CTDi6560.

Phylogenomic databases

eggNOGiKOG2082. Eukaryota.
COG0531. LUCA.
HOGENOMiHOG000092644.
HOVERGENiHBG052852.
InParanoidiQ28677.
KOiK14427.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR000622. KCC1.
IPR000076. KCL_cotranspt.
IPR018491. SLC12_C.
IPR004842. SLC12A_fam.
[Graphical view]
PANTHERiPTHR11827:SF46. PTHR11827:SF46. 4 hits.
PfamiPF00324. AA_permease. 2 hits.
PF03522. SLC12. 2 hits.
[Graphical view]
PRINTSiPR01081. KCLTRNSPORT.
PR01082. KCLTRNSPORT1.
TIGRFAMsiTIGR00930. 2a30. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and functional expression of the K-Cl cotransporter from rabbit, rat, and human. A new member of the cation-chloride cotransporter family."
    Gillen C.M., Brill S., Payne J.A., Forbush B. III
    J. Biol. Chem. 271:16237-16244(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
    Strain: New Zealand white.
    Tissue: Kidney.
  2. "Mouse K-Cl cotransporter KCC1: cloning, mapping, pathological expression, and functional regulation."
    Su W., Shmukler B.E., Chernova M.N., Stuart-Tilley A.K., de Franceschi L., Brugnara C., Alper S.L.
    Am. J. Physiol. 277:C899-C912(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION.

Entry informationi

Entry nameiS12A4_RABIT
AccessioniPrimary (citable) accession number: Q28677
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 13, 2002
Last sequence update: November 1, 1996
Last modified: February 17, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Activated by N-ethylmaleimide (NEM). Inhibited by furosemide and bumetanide.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.