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Q28677 (S12A4_RABIT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Solute carrier family 12 member 4
Alternative name(s):
Electroneutral potassium-chloride cotransporter 1
Erythroid K-Cl cotransporter 1
rbKCC1
Gene names
Name:SLC12A4
Synonyms:KCC1
OrganismOryctolagus cuniculus (Rabbit) [Reference proteome]
Taxonomic identifier9986 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length1085 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. May be involved in the regulation of basolateral Cl- exit in NaCl absorbing epithelia.

Enzyme regulation

Inhibited by WNK3 By similarity.

Subunit structure

Homomultimer and heteromultimer with other K-Cl cotransporters By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Post-translational modification

N-glycosylated. Ref.2

Miscellaneous

Activated by N-ethylmaleimide (NEM). Inhibited by furosemide and bumetanide.

Sequence similarities

Belongs to the SLC12A transporter family.

Ontologies

Keywords
   Biological processIon transport
Potassium transport
Symport
Transport
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   LigandPotassium
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpotassium ion transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionpotassium:chloride symporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10851085Solute carrier family 12 member 4
PRO_0000178032

Regions

Topological domain1 – 118118Cytoplasmic Potential
Transmembrane119 – 13921Helical; Potential
Transmembrane149 – 16921Helical; Potential
Topological domain170 – 21445Cytoplasmic Potential
Transmembrane215 – 23521Helical; Potential
Transmembrane253 – 27321Helical; Potential
Topological domain274 – 2752Cytoplasmic Potential
Transmembrane276 – 29621Helical; Potential
Transmembrane356 – 37621Helical; Potential
Topological domain377 – 40731Cytoplasmic Potential
Transmembrane408 – 42821Helical; Potential
Transmembrane453 – 47321Helical; Potential
Topological domain474 – 49320Cytoplasmic Potential
Transmembrane494 – 51421Helical; Potential
Transmembrane567 – 58721Helical; Potential
Topological domain588 – 62740Cytoplasmic Potential
Transmembrane628 – 64821Helical; Potential
Transmembrane845 – 86521Helical; Potential
Topological domain866 – 1085220Cytoplasmic Potential
Compositional bias160 – 1634Poly-Cys

Amino acid modifications

Modified residue471Phosphoserine By similarity
Modified residue9671Phosphoserine By similarity
Modified residue9831Phosphothreonine By similarity
Glycosylation2451N-linked (GlcNAc...) Potential
Glycosylation3121N-linked (GlcNAc...) Potential
Glycosylation3311N-linked (GlcNAc...) Potential
Glycosylation3471N-linked (GlcNAc...) Potential
Glycosylation4391N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q28677 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 717408738E226B8E

FASTA1,085120,743
        10         20         30         40         50         60 
MPHFTVVPVD GPRRGDYDNL EGLSWVDYGE RAEREDPDGH GNHRESSPFL CPLEASRGSD 

        70         80         90        100        110        120 
YYDRNLALFE EELDIRPKVS SLLGKLVSYT NLTQGAKEHE EAESGEGTRR RAAKAPSMGT 

       130        140        150        160        170        180 
LMGVYLPCLQ NIFGVILFLR LTWMVGTAGV LQALLIVLIC CCCTLLTAIS MSAIATNGVV 

       190        200        210        220        230        240 
PAGGSYFMIS RSLGPEFGGA VGLCFYLGTT FAAAMYILGA IEILLTYIAP PAAIFYPSGT 

       250        260        270        280        290        300 
HDTSNATLNN MRVYGTVFLS FMTLVVFVGV KYVNKFASLF LACVIISILS IYAGGIKSMF 

       310        320        330        340        350        360 
DPPVFPVCML GNRTLSRDQF DICAKTTMVD NETVATRLWS FFCHSPNLTT DSCDPYFLLN 

       370        380        390        400        410        420 
NVTEIPGIPG AAAGVLQENL WSAYLEKGEV VEKRGLPSTD AVGLKENLPL YVVADIATSF 

       430        440        450        460        470        480 
TVLVGIFFPS VTGIMAGSNR SGDLRDAQKS IPVGTILAIV TTSLVYFSSV VLFGACIEGV 

       490        500        510        520        530        540 
VLRDKYGDGV SRNLVVGTLA WPSPWVIVVG SFFSTCGAGL QSLTGAPRLL QAIAKDNIIP 

       550        560        570        580        590        600 
FLRVFGHGKA NGEPTWALLL TALIAELGIL IASLDMVAPI LSMFFLMCYL FVNLACAVQT 

       610        620        630        640        650        660 
LLRTPNWRPR FKYYHWALSF LGMSLCLALM FVSSWYYALV AMLIAGMIYK YIEYQGAEKE 

       670        680        690        700        710        720 
WGDGIRGLSL SAARYALLRL EEGPPHTKNW RPQLLVLLKL DEDLHVKYPR LLTFASQLKA 

       730        740        750        760        770        780 
GKGLTIVGSV IQGSFLESYG EAQAAEQTIK NMMKIEKVKG FCQVVVASKV REGLAHLIQS 

       790        800        810        820        830        840 
CGLGGMRHNS VVLGWPYGWR QSEDPRAWKT FIDTVRCTTA AHLALLVPKN IAFYPSNHER 

       850        860        870        880        890        900 
YLEGHIDVWW IVHDGGMLML LPFLLRQHKV WRKCRMRIFT VAQMDDNSIQ MKKDLAVFLY 

       910        920        930        940        950        960 
HLRLEAEVEV VEMHNSDISA YTYERTLMME QRSQMLRQMR LTKTEREREA QLVKDRHSAL 

       970        980        990       1000       1010       1020 
RLESLYSDEE DEAAAGADKI QMTWTRDKYM TEPWDPSHTP DNFRELVHIK PDQSNVRRMH 

      1030       1040       1050       1060       1070       1080 
TAVKLNEVIV TRSHDARLVL LNMPGPPKNS EGDENYMEFL EVLTEGLERV LLVRGGGREV 


ITIYS

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References

[1]"Molecular cloning and functional expression of the K-Cl cotransporter from rabbit, rat, and human. A new member of the cation-chloride cotransporter family."
Gillen C.M., Brill S., Payne J.A., Forbush B. III
J. Biol. Chem. 271:16237-16244(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
Strain: New Zealand white.
Tissue: Kidney.
[2]"Mouse K-Cl cotransporter KCC1: cloning, mapping, pathological expression, and functional regulation."
Su W., Shmukler B.E., Chernova M.N., Stuart-Tilley A.K., de Franceschi L., Brugnara C., Alper S.L.
Am. J. Physiol. 277:C899-C912(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U55053 mRNA. Translation: AAC48593.1.
PIRT18369.
RefSeqNP_001076172.1. NM_001082703.2.
UniGeneOcu.2291.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRING9986.ENSOCUP00000005495.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100009441.

Organism-specific databases

CTD100009441.

Phylogenomic databases

eggNOGCOG0531.
HOGENOMHOG000092644.
HOVERGENHBG052852.
OrthoDBEOG476JZG.

Family and domain databases

InterProIPR004841. AA-permease_dom.
IPR000622. K/Cl_cotranspt1.
IPR018491. K/Cl_cotranspt_1/3.
IPR000076. KCL_cotranspt.
IPR004842. Na/K/Cl_cotransptS.
[Graphical view]
PfamPF00324. AA_permease. 2 hits.
PF03522. KCl_Cotrans_1. 1 hit.
[Graphical view]
PRINTSPR01081. KCLTRNSPORT.
PR01082. KCLTRNSPORT1.
TIGRFAMsTIGR00930. 2a30. 1 hit.
ProtoNetSearch...

Entry information

Entry nameS12A4_RABIT
AccessionPrimary (citable) accession number: Q28677
Entry history
Integrated into UniProtKB/Swiss-Prot: December 13, 2002
Last sequence update: November 1, 1996
Last modified: April 3, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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