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Protein

Myosin-4

Gene

MYH4

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Muscle contraction.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi179 – 1868ATPSequence analysis

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Muscle protein, Myosin

Keywords - Ligandi

Actin-binding, ATP-binding, Calmodulin-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin-4
Alternative name(s):
Myosin heavy chain 2b
Short name:
MyHC-2b
Myosin heavy chain 4
Myosin heavy chain, skeletal muscle, juvenile
Gene namesi
Name:MYH4
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Thick filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19381938Myosin-4PRO_0000123400Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei36 – 361PhosphoserineBy similarity
Modified residuei64 – 641PhosphothreonineBy similarity
Modified residuei69 – 691PhosphothreonineBy similarity
Modified residuei389 – 3891PhosphotyrosineBy similarity
Modified residuei392 – 3921PhosphoserineBy similarity
Modified residuei419 – 4191PhosphothreonineBy similarity
Modified residuei424 – 4241PhosphotyrosineBy similarity
Modified residuei756 – 7561Pros-methylhistidine1 Publication
Modified residuei1091 – 10911PhosphoserineBy similarity
Modified residuei1095 – 10951PhosphoserineBy similarity
Modified residuei1161 – 11611PhosphoserineBy similarity
Modified residuei1236 – 12361PhosphoserineBy similarity
Modified residuei1240 – 12401PhosphothreonineBy similarity
Modified residuei1242 – 12421PhosphoserineBy similarity
Modified residuei1254 – 12541PhosphothreonineBy similarity
Modified residuei1260 – 12601PhosphoserineBy similarity
Modified residuei1264 – 12641PhosphothreonineBy similarity
Modified residuei1277 – 12771PhosphoserineBy similarity
Modified residuei1285 – 12851PhosphothreonineBy similarity
Modified residuei1287 – 12871PhosphoserineBy similarity
Modified residuei1291 – 12911PhosphoserineBy similarity
Modified residuei1302 – 13021PhosphoserineBy similarity
Modified residuei1305 – 13051PhosphoserineBy similarity
Modified residuei1463 – 14631PhosphotyrosineBy similarity
Modified residuei1466 – 14661PhosphothreonineBy similarity
Modified residuei1473 – 14731PhosphoserineBy similarity
Modified residuei1491 – 14911PhosphotyrosineBy similarity
Modified residuei1494 – 14941PhosphoserineBy similarity
Modified residuei1500 – 15001PhosphothreonineBy similarity
Modified residuei1513 – 15131PhosphoserineBy similarity
Modified residuei1516 – 15161PhosphothreonineBy similarity
Modified residuei1541 – 15411PhosphoserineBy similarity
Modified residuei1553 – 15531PhosphoserineBy similarity
Modified residuei1573 – 15731PhosphoserineBy similarity
Modified residuei1599 – 15991PhosphoserineBy similarity
Modified residuei1602 – 16021PhosphoserineBy similarity
Modified residuei1713 – 17131PhosphoserineBy similarity
Modified residuei1725 – 17251PhosphoserineBy similarity
Modified residuei1729 – 17291PhosphothreonineBy similarity
Modified residuei1735 – 17351PhosphothreonineBy similarity
Modified residuei1738 – 17381PhosphoserineBy similarity
Cross-linki1812 – 1812Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiQ28641.

Interactioni

Subunit structurei

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000017571.

Structurei

3D structure databases

ProteinModelPortaliQ28641.
SMRiQ28641. Positions 841-966.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini86 – 781696Myosin motorAdd
BLAST
Domaini784 – 81330IQPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni658 – 68023Actin-bindingBy similarityAdd
BLAST
Regioni760 – 77415Actin-bindingBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili842 – 19381097Sequence analysisAdd
BLAST

Domaini

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).Curated

Sequence similaritiesi

Contains 1 IQ domain.PROSITE-ProRule annotation
Contains 1 myosin motor domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0161. Eukaryota.
COG5022. LUCA.
HOGENOMiHOG000173959.
HOVERGENiHBG004704.
InParanoidiQ28641.
KOiK10352.

Family and domain databases

Gene3Di4.10.270.10. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00015. IQ. 2 hits.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q28641-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKDADMAIF GEAAPYLRKS EKERIEAQNK PFDAKSSVFV ADPKESFVKA
60 70 80 90 100
TVQSREGGKV TAKTEAGATV TVKEDQVFPM NPPKYDKIED MAMMTHLHEP
110 120 130 140 150
AVLYNLKEXY AAWMIYTYSG LFCVTVNPYK WLPVYNAEVV TAYRGKKRQE
160 170 180 190 200
APPHIFSISD NAYQFMLTDR ENQSILITGE SGAGKTVNTK RVIQYFATIA
210 220 230 240 250
VTGDKKKEEA TSGKMQGTLE DQIISANPLL EAFANAKTVR NDNSSRFGKF
260 270 280 290 300
IRIHFGTTGK LASADIETYL LEKSRVTFQL KAERSYHIFY QIMSNKKPDL
310 320 330 340 350
IEMLLITTNP YDYAFVSQGE ITVPSIDDQE ELMATDSAID ILGFTSDERV
360 370 380 390 400
SIYKLTGAVM HYGNMKFKQK QREEQAEPDG TEVADKAAYL QSLNSXDLLK
410 420 430 440 450
ALCYPRVKVG NEYVTKGQTV QQVYNAVGAL AKAVYEKMFL WMVTRINQQL
460 470 480 490 500
DTKQPRQYFI GVLDIAGFEI FDFNSLEQLC INFTNEKLQQ FFNHHMFVLE
510 520 530 540 550
QEEYKKEGIE WEFIDFGMDL AACIELIEKP MGIFSILEEE CMFPKATDTS
560 570 580 590 600
FKNKLYEQHL GKSNNFQKPK PAKRKVEAHF SLVHYAGTVD YNITGWLDKN
610 620 630 640 650
KDPLNETVVG LYQKSAMKTL AFLFTGTAAA EAEGGGKKGG KKKGSSFQTV
660 670 680 690 700
SALFRENLNK LMTNLRTHSP HFVRSIIPNE TKTPGAMEHE LVLHQLRCNG
710 720 730 740 750
VLEGIRICRK GFPSRILYAD FKQRYKVLNA SAIPEGQFID SKKASEKLLG
760 770 780 790 800
SIDVDHTQYK FGHTKVFFKA GLLGLLEEMR DDKLAQLITR TQAMCRGFLA
810 820 830 840 850
RVEYKKMVER RESIFCIQYN IRAFMNVKHW PWMKLYFKIK PLLKSAETEK
860 870 880 890 900
EMANMKEEFE KTKESLAKAE AKEKELEEKM VALMQEKNDL QLQVQAEADS
910 920 930 940 950
LADAEERCDQ LIKTKIQLEA KIKEVTERAE DEEEINAELT AKKRKLEDEC
960 970 980 990 1000
SELKKDIDDL ELTLAKVEKE KHATENKVKN LTEEMAGLDE TIAKLTKEKK
1010 1020 1030 1040 1050
ALQEAHQQTL DDLQAEEDKV NTLTKAKTKL EQQVDDLEGS LEQEKKIRMD
1060 1070 1080 1090 1100
LERAKRKLEG DLKLAQESTM DIENDKQQLD EKLKKKEFEM SNLQSKIEDE
1110 1120 1130 1140 1150
QALAMQLQKK IKELQARIEE LEEEIEAERA SRAKAEKQRS DLSRELEEIS
1160 1170 1180 1190 1200
ERLEEAGGAT SAQIEMNKKR EAEFQKMRRD LEEATLQHEA TAATLRKKHA
1210 1220 1230 1240 1250
DSVAELGEQI DNLQRVKQKL EKEKSELKME IDDLASNMET VSKAKGNLEK
1260 1270 1280 1290 1300
MCRTLEDQVS ELKTKEEEHQ RLINDLSAQR ARLQTESGEF SRQLDEKDSL
1310 1320 1330 1340 1350
VSQLSRGKQA FTQQIEELKR QLEEEIKAKS ALAHALQSAR HDCDLLREQY
1360 1370 1380 1390 1400
EEEQEAKAEL QRAMSKANSE VAQWRTKYET DAIQRTEELE EAKKKLAQRL
1410 1420 1430 1440 1450
QDAEEHVEAV NAKCASLEKT KQRLQNEVED LMIDVERTNA ACAALDKKQR
1460 1470 1480 1490 1500
NFDKILAEWK HKYEETHAEL EASQKESRSL STEVFKVKNA YEESLDQLET
1510 1520 1530 1540 1550
LKRENKNLQQ EISDLTEQIA EGGKRIHELE KVKKQVEQEK SELQAALEEA
1560 1570 1580 1590 1600
EASLEHEEGK ILRIQLELNQ VKSEIDRKIA EKDEEIDQLK RNHIRVVESM
1610 1620 1630 1640 1650
QSTLDAEIRS RNDAIRIKKK MEGDLNEMEI QLNHANRMAA EALRNYRNTQ
1660 1670 1680 1690 1700
GILKDTQLHL DDALRGQEDL KEQLAMVERR ANLLQAEIEE LRATLEQTER
1710 1720 1730 1740 1750
SRKVAEQELL DASERVQLLH TQNTSLINTK KKLETDISQI QGEMEDIVQE
1760 1770 1780 1790 1800
ARNAEEKAKK AITDAAMMAE ELKKEQDTSA HLERMKKNME QTVKDLQHRL
1810 1820 1830 1840 1850
DEAEQLALKG GKKQIQKLEA RVRELEAEVE SEQKRNVEAV KGLRKHERRV
1860 1870 1880 1890 1900
KELTYQTEED RKNVLRLQDL VDKLQAKVKS YKRQAEEAEE QCNINLSKFR
1910 1920 1930
KLQHELEEAE ERADIAESQV NKLRVKSREV HTKVISEE
Length:1,938
Mass (Da):223,068
Last modified:November 1, 1996 - v1
Checksum:iD8A8A2EC5B182626
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti757 – 7582TQ → QT AA sequence (PubMed:5539225).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32574 mRNA. Translation: AAA74199.1.
PIRiA35557.
A59293.
RefSeqiNP_001103286.1. NM_001109816.1.
UniGeneiOcu.6900.

Genome annotation databases

GeneIDi100125991.
KEGGiocu:100125991.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32574 mRNA. Translation: AAA74199.1.
PIRiA35557.
A59293.
RefSeqiNP_001103286.1. NM_001109816.1.
UniGeneiOcu.6900.

3D structure databases

ProteinModelPortaliQ28641.
SMRiQ28641. Positions 841-966.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000017571.

Proteomic databases

PRIDEiQ28641.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100125991.
KEGGiocu:100125991.

Organism-specific databases

CTDi4619.

Phylogenomic databases

eggNOGiKOG0161. Eukaryota.
COG5022. LUCA.
HOGENOMiHOG000173959.
HOVERGENiHBG004704.
InParanoidiQ28641.
KOiK10352.

Family and domain databases

Gene3Di4.10.270.10. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00015. IQ. 2 hits.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation, sequencing of myosin heavy chain cDNA from rabbit skeletal muscle and a novel cosynthesis of S-1 fragment with the essential and regulatory light chains."
    Maeda K., Hostinova E., Roesch-Kleinkauf A., Schuster H., Gasperik J., Wittinghofer A.
    Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: New Zealand white.
    Tissue: Skeletal muscle.
  2. "Amino acid sequence around the single 3-methylhistidine residue in rabbit skeletal muscle myosin."
    Huszar G., Elzinga M.
    Biochemistry 10:229-236(1971) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 748-760, METHYLATION AT HIS-756.
    Tissue: Skeletal muscle.

Entry informationi

Entry nameiMYH4_RABIT
AccessioniPrimary (citable) accession number: Q28641
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Represents a conventional myosin. This protein should not be confused with the unconventional myosin-4 (MYO4).Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.