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Protein

Na(+)/H(+) exchange regulatory cofactor NHE-RF1

Gene

SLC9A3R1

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Involved in the regulation of phosphate reabsorption in the renal proximal tubules (By similarity).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processWnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Na(+)/H(+) exchange regulatory cofactor NHE-RF1
Short name:
NHERF-1
Alternative name(s):
Ezrin-radixin-moesin-binding phosphoprotein 50
Short name:
EBP50
Regulatory cofactor of Na(+)/H(+) exchanger
Sodium-hydrogen exchanger regulatory factor 1
Solute carrier family 9 isoform A3 regulatory factor 1
Gene namesi
Name:SLC9A3R1
Synonyms:NHERF, NHERF1
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi287S → A: Abolishes phosphorylation; when associated with A-289 and A-290. 1 Publication1
Mutagenesisi289 – 290SS → AA: Abolishes phosphorylation; when associated with A-287. 1 Publication2
Mutagenesisi289S → D: Enhances dimerization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000968012 – 358Na(+)/H(+) exchange regulatory cofactor NHE-RF1Add BLAST357

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Modified residuei46PhosphoserineBy similarity1
Modified residuei279PhosphoserineBy similarity1
Modified residuei289Phosphoserine1 Publication1
Modified residuei290Phosphoserine1 Publication1
Modified residuei292PhosphothreonineBy similarity1
Modified residuei293PhosphoserineBy similarity1
Modified residuei298PhosphoserineBy similarity1
Modified residuei301PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on serine residues.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiQ28619.

PTM databases

iPTMnetiQ28619.

Expressioni

Tissue specificityi

Detected in ileum, duodenum and in kidney, where it is found in the glomerulus, the proximal tubule, the thick ascending limb of Henle's loop and the cortical collecting duct.1 Publication

Interactioni

Subunit structurei

Homodimer, and heterodimer with SLC9A3R2. Binds the N-termini of EZR, RDX and MSN. Binds the C-termini of PDGFRA, PDGFRB, ADRB2, NOS2, CFTR and PAG1. In resting T-cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation. Binds ARHGAP17, EPI64, RACK1, OPRK1, GNAQ, CTNNB1, PLCB3, PDZK1 and CLCN3. Forms a complex with CFTR and SLC4A7. Forms a complex with SLC4A7 and ATP6V1B1. Directly interacts with HTR4 (By similarity). Interacts with TRPC4 (via the PDZ-binding domain) (By similarity). Interacts (via the PDZ 1 domain) with PODXL (via the C-terminal PDZ-binding motif DTHL); interaction is not detected in glomerular epithelium cells (By similarity). Interacts (via the PDZ 1 domain) with PODXL (via the C-terminal PDZ-binding motif DTHL); the interaction take place early in the secretory pathway and is necessary for its apical membrane sorting (By similarity). Interacts with SLC34A1 (By similarity). Interacts (via the PDZ domains) with SLC26A6 (By similarity). Interacts with SLC26A3 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Grk6O70293-15EBI-7073613,EBI-7073604From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi1172218. 1 interactor.
IntActiQ28619. 2 interactors.
MINTiMINT-148118.

Structurei

3D structure databases

ProteinModelPortaliQ28619.
SMRiQ28619.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 94PDZ 1PROSITE-ProRule annotationAdd BLAST81
Domaini154 – 234PDZ 2PROSITE-ProRule annotationAdd BLAST81

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG052616.
InParanoidiQ28619.
KOiK13365.

Family and domain databases

InterProiView protein in InterPro
IPR015098. EBP50_C-term.
IPR031199. NHERF-1.
IPR017300. NHERF-1/NHERF-2.
IPR001478. PDZ.
PANTHERiPTHR14191:SF14. PTHR14191:SF14. 1 hit.
PfamiView protein in Pfam
PF09007. EBP50_C. 1 hit.
PF00595. PDZ. 2 hits.
PIRSFiPIRSF037866. EBP50. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD283022. EBP50_C-term. 1 hit.
SMARTiView protein in SMART
SM00228. PDZ. 2 hits.
SUPFAMiSSF50156. SSF50156. 2 hits.
PROSITEiView protein in PROSITE
PS50106. PDZ. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q28619-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSADAAAGAP LPRLCCLEKG PNGYGFHLHG EKGKVGQYIR LVEPGSPAEK
60 70 80 90 100
AGLLAGDRLV EVNGENVEKE THQQVVSRIR AALNAVRLLV VDPDTDEQFR
110 120 130 140 150
KLGVQIRGEL LRAQAGPEQA GPPAAPGEQG PAGENEPREV EKSHPERREL
160 170 180 190 200
RPRLCAMKKG PNGYGFNLHS DKSRPGQFIR AVDPDSPAEA SGLREQDRIV
210 220 230 240 250
EVNGVCVEGK QHGDVVTAIK AGGDEAKLLV VDKETDEFFK KCKVVPSSEH
260 270 280 290 300
LNGPLPEPFT NGEIQKNNPE TLAPAASESP RPALARSASS DTSEELASQD
310 320 330 340 350
SPKKEDSTAP SSTSSSSDPI LDFSISLAVA KERAHQKRSS RRAPQMDWSE

KKELFSNL
Length:358
Mass (Da):38,562
Last modified:January 23, 2007 - v3
Checksum:iF55E6D6FFA01B991
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19815 mRNA. Translation: AAA80218.1.
PIRiI46532.
RefSeqiNP_001075814.1. NM_001082345.1.
UniGeneiOcu.3088.

Genome annotation databases

GeneIDi100009196.
KEGGiocu:100009196.

Similar proteinsi

Entry informationi

Entry nameiNHRF1_RABIT
AccessioniPrimary (citable) accession number: Q28619
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 119 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome