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Protein

Voltage-gated potassium channel subunit beta-1

Gene

KCNAB1

Organism
Mustela putorius (European polecat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Cytoplasmic potassium channel subunit that modulates the characteristics of the channel-forming alpha-subunits (By similarity). Modulates action potentials via its effect on the pore-forming alpha subunits (By similarity). Promotes expression of the pore-forming alpha subunits at the cell membrane, and thereby increases channel activity (By similarity). Mediates closure of delayed rectifier potassium channels by physically obstructing the pore via its N-terminal domain and increases the speed of channel closure for other family members (By similarity). Promotes the closure of KCNA1, KCNA2 and KCNA5 channels (By similarity). Accelerates KCNA4 channel closure (PubMed:7890764). Accelerates the closure of heteromeric channels formed by KCNA1 and KCNA4 (By similarity). Accelerates the closure of heteromeric channels formed by KCNA2, KCNA5 and KCNA6 (By similarity). Enhances KCNB1 and KCNB2 channel activity (By similarity). Binds NADPH; this is required for efficient down-regulation of potassium channel activity (By similarity). Has NADPH-dependent aldoketoreductase activity (By similarity). Oxidation of the bound NADPH strongly decreases N-type inactivation of potassium channel activity (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei104NADPBy similarity1
Binding sitei126NADPBy similarity1
Active sitei131Proton donor/acceptorBy similarity1
Binding sitei131NADPBy similarity1
Binding sitei255NADPBy similarity1
Binding sitei295NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi97 – 98NADPBy similarity2
Nucleotide bindingi229 – 230NADPBy similarity2
Nucleotide bindingi284 – 289NADPBy similarity6
Nucleotide bindingi364 – 370NADPBy similarity7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Oxidoreductase, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

NADP, Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-gated potassium channel subunit beta-1 (EC:1.1.1.-By similarity)
Alternative name(s):
K(+) channel subunit beta-1
Kv-beta-1
Gene namesi
Name:KCNAB1
Synonyms:KVB3
OrganismiMustela putorius (European polecat)
Taxonomic identifieri9668 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaMustelidaeMustelinaeMustela

Subcellular locationi

  • Cytoplasm By similarity
  • Membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
  • Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity

  • Note: Recruited to the cytoplasmic side of the cell membrane via its interaction with pore-forming potassium channel alpha subunits.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001487411 – 408Voltage-gated potassium channel subunit beta-1Add BLAST408

Expressioni

Tissue specificityi

Expression most abundant in aorta. Also high in left ventricle. Also detected in right ventricle, atrium, brain, skeletal muscle and kidney. Not detected in liver.1 Publication

Interactioni

Subunit structurei

Homotetramer (By similarity). Interaction with tetrameric potassium channel alpha subunits gives rise to a heterooctamer (Probable). Identified in potassium channel complexes containing KCNA1, KCNA2, KCNA4, KCNA5, KCNA6, KCNAB1 and KCNAB2 (By similarity). Interacts with KCNA1 (By similarity). Interacts with the dimer formed by GNB1 and GNG2; this enhances KCNA1 binding (By similarity). Interacts with KCNA4 (By similarity). Interacts with KCNA5 (By similarity). Interacts with KCNB2 (By similarity). Interacts with SQSTM1 (By similarity). Part of a complex containing KCNA1, KCNA4 and LGI1; interaction with LGI1 inhibits down-regulation of KCNA1 channel activity (By similarity).By similarityCurated

Protein-protein interaction databases

STRINGi9669.ENSMPUP00000002766.

Structurei

3D structure databases

ProteinModelPortaliQ28528.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal domain of the beta subunit mediates closure of delayed rectifier potassium channels by physically obstructing the pore.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1575. Eukaryota.
COG0667. LUCA.
HOVERGENiHBG052216.

Family and domain databases

CDDicd06660. Aldo_ket_red. 1 hit.
Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR005983. K_chnl_volt-dep_bsu_KCNAB.
IPR005399. K_chnl_volt-dep_bsu_KCNAB-rel.
IPR005400. K_chnl_volt-dep_bsu_KCNAB1.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 1 hit.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PRINTSiPR01578. KCNAB1CHANEL.
PR01577. KCNABCHANNEL.
SUPFAMiSSF51430. SSF51430. 1 hit.
TIGRFAMsiTIGR01293. Kv_beta. 1 hit.

Sequencei

Sequence statusi: Complete.

Q28528-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLYKPACAD IPSPKLGLPK SSESALKCRR HLAVTKPPPQ AACWPARPSG
60 70 80 90 100
AAERKFLEKF LRVHGISLQE TTRAETGMAY RNLGKSGLRV SCLGLGTWVT
110 120 130 140 150
FGGQISDEVA ERLMTIAYES GVNLFDTAEV YAAGKAEVIL GSIIKKKGWR
160 170 180 190 200
RSSLVITTKL YWGGKAETER GLSRKHIIEG LKGSLQRLQL EYVDVVFANR
210 220 230 240 250
PDSNTPMEEI VRAMTHVINQ GMAMYWGTSR WSAMEIMEAY SVARQFNMIP
260 270 280 290 300
PVCEQAEYHL FQREKVEVQL PELYHKIGVG AMTWSPLACG IISGKYGNGV
310 320 330 340 350
PESSRASLKC YQWLKERIVS EEGRKQQNKL KDLSPIAERL GCTLPQLAVA
360 370 380 390 400
WCLRNEGVSS VLLGSSTPEQ LIENLGAIQV LPKMTSHVVN EIDNILRNKP

YSKKDYRS
Length:408
Mass (Da):45,483
Last modified:November 1, 1996 - v1
Checksum:iAB74664C1AB15F30
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17966 mRNA. Translation: AAC48462.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17966 mRNA. Translation: AAC48462.1.

3D structure databases

ProteinModelPortaliQ28528.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9669.ENSMPUP00000002766.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG1575. Eukaryota.
COG0667. LUCA.
HOVERGENiHBG052216.

Family and domain databases

CDDicd06660. Aldo_ket_red. 1 hit.
Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR005983. K_chnl_volt-dep_bsu_KCNAB.
IPR005399. K_chnl_volt-dep_bsu_KCNAB-rel.
IPR005400. K_chnl_volt-dep_bsu_KCNAB1.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 1 hit.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PRINTSiPR01578. KCNAB1CHANEL.
PR01577. KCNABCHANNEL.
SUPFAMiSSF51430. SSF51430. 1 hit.
TIGRFAMsiTIGR01293. Kv_beta. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKCAB1_MUSPU
AccessioniPrimary (citable) accession number: Q28528
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: November 1, 1996
Last modified: October 5, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.