Q28514 (GSTP1_MACMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase P
EC=2.5.1.18

Alternative name(s):
GST class-pi

Gene names

Name:

GSTP1

Organism

Macaca mulatta (Rhesus macaque) [Reference proteome]

Taxonomic identifier

9544 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Cercopithecoidea › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration By similarity.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer By similarity. Interacts with CDK5 By similarity.
Subcellular location	Cytoplasm By similarity. Mitochondrion By similarity. Nucleus By similarity. Note: The 83 N-terminal amino-acids function as un uncleaved transit peptide, and arginine residues within it are crucial for motochondrial localization By similarity.
Sequence similarities	Belongs to the GST superfamily. Pi family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm Mitochondrion Nucleus
Molecular function	Transferase
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutathione metabolic process Inferred from sequence or structural similarity. Source: UniProtKB xenobiotic metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutathione transferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 210	209	Glutathione S-transferase P		PRO_0000185901
Regions
	Domain	2 – 81	80	GST N-terminal
	Domain	83 – 204	122	GST C-terminal
	Region	52 – 53	2	Glutathione binding By similarity
	Region	65 – 66	2	Glutathione binding By similarity
Sites
	Binding site	8	1	Glutathione By similarity
	Binding site	14	1	Glutathione By similarity
	Binding site	39	1	Glutathione By similarity
	Binding site	45	1	Glutathione By similarity
Amino acid modifications
	Modified residue	4	1	Phosphotyrosine; by EGFR By similarity
	Modified residue	109	1	Phosphotyrosine By similarity
	Modified residue	128	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q28514 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 4DE128B6A6C45434	FASTA	210	23,438
	10 20 30 40 50 60 MPPYTVVYFP VRGRCAALRM LLADQGQSWK EEVVTMETWQ EGSLKASCLY GQLPKFQDGD 70 80 90 100 110 120 LTLYQSNTFL RHLGRTLGLY GKDQREAALV DMVNDGVEDL RCKYLSLIYT NYEAGKDDYV 130 140 150 160 170 180 KALPGQLKPF ETLLSQNQGG KTFIVGDQIS FADYNLLDLL LIHEVLAPGC LDAFPLLSAY 190 200 210 VARLSARPKL KAFLASPEHV NLPINGNGKQ

References

[1]

"The mRNA for GST Pi from FRHK rhesus monkey kidney cells codes for an enzyme with activity towards 1-chloro-2,4-dinitrobenzene in spite of an I68F mutation." Swedmark S., Morgenstern R., Weinander R. Gene 206:99-105(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L49501 mRNA. Translation: AAC37605.1.

PIR

JC6529.

RefSeq

NP_001036141.1. NM_001042676.1.

UniGene

Mmu.3442.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LX2	model	-	A	1-210	[»]

ProteinModelPortal

Q28514.

SMR

Q28514. Positions 1-210.

ModBase

Search...

Proteomic databases

PRIDE

Q28514.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

721704.

KEGG

mcc:721704.

Organism-specific databases

CTD

2950.

Phylogenomic databases

HOVERGEN

HBG108324.

KO

K00799.

Family and domain databases

Gene3D

1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.

InterPro

IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR017933. Glutathione_S_Trfase/Cl_chnl_C.
IPR004046. GST_C.
IPR003082. GST_pi.
IPR012336. Thioredoxin-like_fold.
[Graphical view]

Pfam

PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]

PRINTS

PR01268. GSTRNSFRASEP.

SUPFAM

SSF47616. GST_C_like. 1 hit.
SSF52833. Thiordxn-like_fd. 1 hit.

PROSITE

PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

19966157.

Entry information

Entry name

GSTP1_MACMU

Accession

Primary (citable) accession number: Q28514

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	March 6, 2013
This is version 87 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program