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Protein

Beta-2 adrenergic receptor

Gene

ADRB2

Organism
Macaca mulatta (Rhesus macaque)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei113 – 1131Agonist or antagonistBy similarity
Binding sitei118 – 1181Agonist or antagonistBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-2 adrenergic receptor
Alternative name(s):
Beta-2 adrenoreceptor
Short name:
Beta-2 adrenoceptor
Gene namesi
Name:ADRB2
Synonyms:B2AR
OrganismiMacaca mulatta (Rhesus macaque)
Taxonomic identifieri9544 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca
Proteomesi
  • UP000006718 Componenti: Chromosome 6

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein By similarity
  • Early endosome By similarity

  • Note: Colocalizes with VHL on cell membranes. Activated receptors are internalized into endosomes prior to their degradation in lysosomes.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3434ExtracellularBy similarityAdd
BLAST
Transmembranei35 – 5824Helical; Name=1By similarityAdd
BLAST
Topological domaini59 – 7113CytoplasmicBy similarityAdd
BLAST
Transmembranei72 – 9524Helical; Name=2By similarityAdd
BLAST
Topological domaini96 – 10611ExtracellularBy similarityAdd
BLAST
Transmembranei107 – 12923Helical; Name=3By similarityAdd
BLAST
Topological domaini130 – 15021CytoplasmicBy similarityAdd
BLAST
Transmembranei151 – 17424Helical; Name=4By similarityAdd
BLAST
Topological domaini175 – 19622ExtracellularBy similarityAdd
BLAST
Transmembranei197 – 22024Helical; Name=5By similarityAdd
BLAST
Topological domaini221 – 27454CytoplasmicBy similarityAdd
BLAST
Transmembranei275 – 29824Helical; Name=6By similarityAdd
BLAST
Topological domaini299 – 3057ExtracellularBy similarity
Transmembranei306 – 32924Helical; Name=7By similarityAdd
BLAST
Topological domaini330 – 41586CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 415415Beta-2 adrenergic receptorPRO_0000069131Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi6 – 61N-linked (GlcNAc...)Sequence analysis
Glycosylationi15 – 151N-linked (GlcNAc...)Sequence analysis
Disulfide bondi106 ↔ 191PROSITE-ProRule annotation
Modified residuei141 – 1411PhosphotyrosineBy similarity
Disulfide bondi184 ↔ 190PROSITE-ProRule annotation
Modified residuei246 – 2461PhosphoserineBy similarity
Modified residuei261 – 2611Phosphoserine; by PKASequence analysis
Modified residuei262 – 2621Phosphoserine; by PKASequence analysis
Lipidationi341 – 3411S-palmitoyl cysteineBy similarity
Modified residuei345 – 3451Phosphoserine; by PKABy similarity
Modified residuei346 – 3461Phosphoserine; by PKABy similarity
Modified residuei355 – 3551Phosphoserine; by BARKCurated
Modified residuei356 – 3561Phosphoserine; by BARKCurated
Modified residuei384 – 38414-hydroxyprolineBy similarity
Modified residuei397 – 39714-hydroxyprolineBy similarity

Post-translational modificationi

Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation (By similarity).By similarity
Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK.
Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.By similarity
Polyubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation. Ubiquitination by the VHL-E3 ligase complex is oxygen-dependent (By similarity).By similarity
Hydroxylation by EGLN3 occurs only under normoxia and increases the interaction with VHL and the subsequent ubiquitination and degradation of ADRB2.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Hydroxylation, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

Expressioni

Gene expression databases

ExpressionAtlasiQ28509. baseline.

Interactioni

Subunit structurei

Binds SLC9A3R1 and GPRASP1. Interacts with ARRB1 and ARRB2. Interacts with SRC, USP20 and USP33. Interacts with VHL; the interaction, which is increased on hydroxylation of ADRB2, ubiquitinates ADRB2 leading to its degradation. Interacts with EGLN3; the interaction hydroxylates ADRB2 facilitating VHL-E3 ligase-mediated ubiquitination. Interacts (via PDZ-binding motif) with SNX27 (via PDZ domain); the interaction is required when endocytosed to prevent degradation in lysosomes and promote recycling to the plasma membrane. Interacts with CNIH4. Interacts with ARRDC3.By similarity

Protein-protein interaction databases

STRINGi9544.ENSMMUP00000002958.

Structurei

3D structure databases

ProteinModelPortaliQ28509.
SMRiQ28509. Positions 29-348.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni193 – 20715Agonist and antagonist bindingBy similarityAdd
BLAST
Regioni286 – 2938Agonist and antagonist bindingBy similarity
Regioni312 – 3165Agonist and antagonist bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi412 – 4154PDZ-binding

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118774.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiQ28509.
KOiK04142.
OMAiGRFHAQN.
OrthoDBiEOG7BS4BS.
TreeFamiTF316350.

Family and domain databases

InterProiIPR002233. ADR_fam.
IPR000332. ADRB2_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24248:SF21. PTHR24248:SF21. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01103. ADRENERGICR.
PR00562. ADRENRGCB2AR.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q28509-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQPGNGSAF LLAPNGSHAP DHDVTQERDE AWVVGMGIVM SLIVLAIVFG
60 70 80 90 100
NVLVITAIAK FERLQTVTNY FITSLACADL VMGLAVVPFG AAHILMKMWT
110 120 130 140 150
FGNFWCEFWT SIDVLCVTAS IETLCVIAVD RYFAITSPFK YQSLLTKNKA
160 170 180 190 200
RVIILMVWIV SGLTSFLPIQ MHWYRATHQE AINCYAKETC CDFFTNQAYA
210 220 230 240 250
IASSIVSFYV PLVIMVFVYS RVFQEAKRQL QKIDKSEGRF HAQNLSQVEQ
260 270 280 290 300
DGRTGHGLRR SSKFCLKEHK ALKTLGIIMG TFTLCWLPFF IVNIVHVIQD
310 320 330 340 350
NLIPKEVYIL LNWVGYVNSG FNPLIYCRSP DFRIAFQELL CLRRSSLKAC
360 370 380 390 400
GNGYSSNSNG NTGEQSGYHL EQEKENKLLC EDLPGTEDFV GHQGTVPSDN
410
IDSQGRSCST NDSLL
Length:415
Mass (Da):46,472
Last modified:November 1, 1996 - v1
Checksum:iF208BD7570FE59B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38905 mRNA. Translation: AAC41914.1.
PIRiI53040.
RefSeqiNP_001036239.1. NM_001042774.1.
UniGeneiMmu.3441.

Genome annotation databases

EnsembliENSMMUT00000003133; ENSMMUP00000002958; ENSMMUG00000002214.
GeneIDi710146.
KEGGimcc:710146.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38905 mRNA. Translation: AAC41914.1.
PIRiI53040.
RefSeqiNP_001036239.1. NM_001042774.1.
UniGeneiMmu.3441.

3D structure databases

ProteinModelPortaliQ28509.
SMRiQ28509. Positions 29-348.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9544.ENSMMUP00000002958.

Protein family/group databases

GPCRDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMMUT00000003133; ENSMMUP00000002958; ENSMMUG00000002214.
GeneIDi710146.
KEGGimcc:710146.

Organism-specific databases

CTDi154.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118774.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiQ28509.
KOiK04142.
OMAiGRFHAQN.
OrthoDBiEOG7BS4BS.
TreeFamiTF316350.

Gene expression databases

ExpressionAtlasiQ28509. baseline.

Family and domain databases

InterProiIPR002233. ADR_fam.
IPR000332. ADRB2_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24248:SF21. PTHR24248:SF21. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01103. ADRENERGICR.
PR00562. ADRENRGCB2AR.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning, sequencing, and expression of the rhesus monkey beta 2 adrenergic receptor."
    Amend A.M., Guan X.-M.
    DNA Cell Biol. 14:753-757(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiADRB2_MACMU
AccessioniPrimary (citable) accession number: Q28509
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.