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Q28503 (PIT1_MACMU) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pituitary-specific positive transcription factor 1

Short name=PIT-1
Alternative name(s):
Growth hormone factor 1
Short name=GHF-1
Gene names
Name:POU1F1
Synonyms:GHF1, PIT1
OrganismMacaca mulatta (Rhesus macaque) [Reference proteome]
Taxonomic identifier9544 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Protein attributes

Sequence length291 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3' By similarity.

Subcellular location

Nucleus.

Sequence similarities

Belongs to the POU transcription factor family. Class-1 subfamily.

Contains 1 homeobox DNA-binding domain.

Contains 1 POU-specific domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainHomeobox
   LigandDNA-binding
   Molecular functionActivator
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtranscription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionsequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform B (identifier: Q28503-1)

Also known as: PIT-1B; GHF-1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform A (identifier: Q28503-2)

Also known as: PIT-1A; GHF-2;

The sequence of this isoform differs from the canonical sequence as follows:
     47-47: T → TVPSILSLIQTPKCLCTHFLVTTLGNT
Note: Altered in its ability to trans-activate compared to isoform B.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 291291Pituitary-specific positive transcription factor 1
PRO_0000100698

Regions

Domain124 – 19875POU-specific
DNA binding214 – 27360Homeobox

Natural variations

Alternative sequence471T → TVPSILSLIQTPKCLCTHFL VTTLGNT in isoform A.
VSP_002315

Sequences

Sequence LengthMass (Da)Tools
Isoform B (PIT-1B) (GHF-1) [UniParc].

Last modified April 27, 2001. Version 2.
Checksum: 79ACFBAD0C8DED15

FASTA29132,959
        10         20         30         40         50         60 
MSCQAFTSAD TFIPLNSDAS ATLPLIMHHS AAECLPVSNH ATNVMSTATG LHYSVPSCHY 

        70         80         90        100        110        120 
GNQPSTYGVM AGSLTLVFYK FPDHTLSHGF PPIHQPLLAE DPTAADFKQE LRRKSKLVEE 

       130        140        150        160        170        180 
PIDMDSPEIR ELEKFANEFK VRRIKLGYTQ TNVGEALAAV HGSEFSQTTI CRFENLQLSF 

       190        200        210        220        230        240 
KNACKLKAIL SKWLEEAEQV GALYNEKVGA NERKRKRRTT ISIAAKDALE RHFGEQNKPS 

       250        260        270        280        290 
SQEIMRMAEE LNLEKEVVRV WFCNRRQREK RVKTSLNQSL FSISKEHLEC R 

« Hide

Isoform A (PIT-1A) (GHF-2) [UniParc].

Checksum: 8B485DEDB142F396
Show »

FASTA31735,741

References

[1]"Pit-1/growth hormone factor 1 splice variant expression in the rhesus monkey pituitary gland and the rhesus and human placenta."
Schanke J.T., Conwell C.M., Durning M., Fisher J.M., Golos T.G.
J. Clin. Endocrinol. Metab. 82:800-807(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Pituitary.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U53566 mRNA. Translation: AAB51352.1.
RefSeqNP_001036325.1. NM_001042860.1.
UniGeneMmu.3411.

3D structure databases

ProteinModelPortalQ28503.
SMRQ28503. Positions 129-273.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9544.ENSMMUP00000021350.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID719349.
KEGGmcc:719349.

Organism-specific databases

CTD5449.

Phylogenomic databases

eggNOGNOG241031.
HOVERGENHBG053120.
KOK09363.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR015586. Pit_1.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PANTHERPTHR11636:SF17. PTHR11636:SF17. 1 hit.
PfamPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSPR00028. POUDOMAIN.
SMARTSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio19971919.

Entry information

Entry namePIT1_MACMU
AccessionPrimary (citable) accession number: Q28503
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: March 19, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families