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Q28480

- IDH3A_MACFA

UniProt

Q28480 - IDH3A_MACFA

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Protein

Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial

Gene

IDH3A

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

Cofactori

Binds 1 magnesium or manganese ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei96 – 961SubstrateBy similarity
Binding sitei106 – 1061SubstrateBy similarity
Binding sitei127 – 1271SubstrateBy similarity
Sitei134 – 1341Critical for catalysisBy similarity
Sitei181 – 1811Critical for catalysisBy similarity
Metal bindingi214 – 2141Magnesium or manganeseBy similarity
Binding sitei214 – 2141SubstrateBy similarity
Metal bindingi238 – 2381Magnesium or manganeseBy similarity
Metal bindingi242 – 2421Magnesium or manganeseBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 4229NADSequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. isocitrate dehydrogenase (NAD+) activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro
  3. NAD binding Source: InterPro

GO - Biological processi

  1. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial (EC:1.1.1.41)
Alternative name(s):
Isocitric dehydrogenase subunit alpha
NAD(+)-specific ICDH subunit alpha
Gene namesi
Name:IDH3A
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei‹1 – 8›8MitochondrionBy similarity
Chaini9 – 347339Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrialPRO_0000014437Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei58 – 581N6-succinyllysineBy similarity
Modified residuei204 – 2041N6-acetyllysineBy similarity
Modified residuei324 – 3241N6-acetyllysine; alternateBy similarity
Modified residuei324 – 3241N6-succinyllysine; alternateBy similarity
Modified residuei331 – 3311N6-succinyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ28480.

Interactioni

Subunit structurei

Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1.By similarity

Protein-protein interaction databases

IntActiQ28480. 1 interaction.
MINTiMINT-6732892.

Structurei

3D structure databases

ProteinModelPortaliQ28480.
SMRiQ28480. Positions 8-343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOVERGENiHBG052080.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q28480-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
QKQVTRGFTG GVQTVTLIPG DGIGPEISAA VMKIFDAAKA PIQWEERNVT
60 70 80 90 100
AIQGPGGKWM IPSEAKESMD KNKMGLKGPL KTPIAAGHPS MNLLLRKTFD
110 120 130 140 150
LYANVRPCVS IEGYKTPYTD VNIVTIRENT EGEYSGIEHV IVDGVVQSIK
160 170 180 190 200
LITEGGSKRI AEFAFEYARN NHRSNVTAVH KANIMRMSDG LFLQKCREVA
210 220 230 240 250
ENCKDIKFNE MYLDTVCLNM VQDPSQFDVL VMPNLYGDIL SDLCAGLIGG
260 270 280 290 300
LGVTPSGNIG ANGVAIFESV HGTAPDIAGK DMANPTALLL SAVMMLRHMG
310 320 330 340
LFDHAARIEA ACFATIKDGK SLTKDLGGNA KCSDFTEEIC RRVKDLD
Length:347
Mass (Da):37,598
Last modified:July 15, 1998 - v2
Checksum:i9100B663642131B9
GO

Sequence cautioni

The sequence CAA60637.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X87172 mRNA. Translation: CAA60637.1. Different initiation.
PIRiS58664.
UniGeneiMfa.6661.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X87172 mRNA. Translation: CAA60637.1 . Different initiation.
PIRi S58664.
UniGenei Mfa.6661.

3D structure databases

ProteinModelPortali Q28480.
SMRi Q28480. Positions 8-343.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q28480. 1 interaction.
MINTi MINT-6732892.

Proteomic databases

PRIDEi Q28480.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG052080.

Family and domain databases

Gene3Di 3.40.718.10. 1 hit.
InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view ]
PANTHERi PTHR11835. PTHR11835. 1 hit.
Pfami PF00180. Iso_dh. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00175. mito_nad_idh. 1 hit.
PROSITEi PS00470. IDH_IMDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and deduced amino acid sequences of the alpha- and beta-subunits of mammalian NAD(+)-isocitrate dehydrogenase."
    Nichols B.J., Perry A.C.F., Hall L., Denton R.M.
    Biochem. J. 310:917-922(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiIDH3A_MACFA
AccessioniPrimary (citable) accession number: Q28480
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 15, 1998
Last modified: October 29, 2014
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3