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Q28480

- IDH3A_MACFA

UniProt

Q28480 - IDH3A_MACFA

Protein

Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial

Gene

IDH3A

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 2 (15 Jul 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei96 – 961SubstrateBy similarity
    Binding sitei106 – 1061SubstrateBy similarity
    Binding sitei127 – 1271SubstrateBy similarity
    Sitei134 – 1341Critical for catalysisBy similarity
    Sitei181 – 1811Critical for catalysisBy similarity
    Metal bindingi214 – 2141Magnesium or manganeseBy similarity
    Binding sitei214 – 2141SubstrateBy similarity
    Metal bindingi238 – 2381Magnesium or manganeseBy similarity
    Metal bindingi242 – 2421Magnesium or manganeseBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi14 – 4229NADSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NAD+) activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial (EC:1.1.1.41)
    Alternative name(s):
    Isocitric dehydrogenase subunit alpha
    NAD(+)-specific ICDH subunit alpha
    Gene namesi
    Name:IDH3A
    OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
    Taxonomic identifieri9541 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei‹1 – 8›8MitochondrionBy similarity
    Chaini9 – 347339Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrialPRO_0000014437Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei58 – 581N6-succinyllysineBy similarity
    Modified residuei204 – 2041N6-acetyllysineBy similarity
    Modified residuei324 – 3241N6-acetyllysine; alternateBy similarity
    Modified residuei324 – 3241N6-succinyllysine; alternateBy similarity
    Modified residuei331 – 3311N6-succinyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiQ28480.

    Interactioni

    Subunit structurei

    Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1.By similarity

    Protein-protein interaction databases

    IntActiQ28480. 1 interaction.
    MINTiMINT-6732892.

    Structurei

    3D structure databases

    ProteinModelPortaliQ28480.
    SMRiQ28480. Positions 8-343.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    HOVERGENiHBG052080.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR004434. Isocitrate_DH_NAD.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11835. PTHR11835. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q28480-1 [UniParc]FASTAAdd to Basket

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    QKQVTRGFTG GVQTVTLIPG DGIGPEISAA VMKIFDAAKA PIQWEERNVT    50
    AIQGPGGKWM IPSEAKESMD KNKMGLKGPL KTPIAAGHPS MNLLLRKTFD 100
    LYANVRPCVS IEGYKTPYTD VNIVTIRENT EGEYSGIEHV IVDGVVQSIK 150
    LITEGGSKRI AEFAFEYARN NHRSNVTAVH KANIMRMSDG LFLQKCREVA 200
    ENCKDIKFNE MYLDTVCLNM VQDPSQFDVL VMPNLYGDIL SDLCAGLIGG 250
    LGVTPSGNIG ANGVAIFESV HGTAPDIAGK DMANPTALLL SAVMMLRHMG 300
    LFDHAARIEA ACFATIKDGK SLTKDLGGNA KCSDFTEEIC RRVKDLD 347
    Length:347
    Mass (Da):37,598
    Last modified:July 15, 1998 - v2
    Checksum:i9100B663642131B9
    GO

    Sequence cautioni

    The sequence CAA60637.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X87172 mRNA. Translation: CAA60637.1. Different initiation.
    PIRiS58664.
    UniGeneiMfa.6661.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X87172 mRNA. Translation: CAA60637.1 . Different initiation.
    PIRi S58664.
    UniGenei Mfa.6661.

    3D structure databases

    ProteinModelPortali Q28480.
    SMRi Q28480. Positions 8-343.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q28480. 1 interaction.
    MINTi MINT-6732892.

    Proteomic databases

    PRIDEi Q28480.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG052080.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR004434. Isocitrate_DH_NAD.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11835. PTHR11835. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00175. mito_nad_idh. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and deduced amino acid sequences of the alpha- and beta-subunits of mammalian NAD(+)-isocitrate dehydrogenase."
      Nichols B.J., Perry A.C.F., Hall L., Denton R.M.
      Biochem. J. 310:917-922(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Testis.

    Entry informationi

    Entry nameiIDH3A_MACFA
    AccessioniPrimary (citable) accession number: Q28480
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 15, 1998
    Last modified: October 1, 2014
    This is version 80 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3