Reviewed, UniProtKB/Swiss-Prot Q28480 (IDH3A_MACFA)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
    EC=1.1.1.41
Alternative name(s):
    Isocitric dehydrogenase
    NAD(+)-specific ICDH

Gene names

Name:

IDH3A

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	347 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Isocitrate + NAD(+) = 2-oxoglutarate + CO(2) + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1 By similarity.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from electronic annotation. Source: InterPro
Molecular function	isocitrate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	‹1 – 8	›8	Mitochondrion By similarity
	Chain	9 – 347	339	Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial		PRO_0000014437
Regions
	Nucleotide binding	14 – 42	29	NAD Potential
Sites
	Metal binding	214	1	Magnesium or manganese By similarity
	Metal binding	238	1	Magnesium or manganese By similarity
	Metal binding	242	1	Magnesium or manganese By similarity
	Binding site	96	1	Substrate By similarity
	Binding site	106	1	Substrate By similarity
	Binding site	127	1	Substrate By similarity
	Binding site	214	1	Substrate By similarity
	Site	134	1	Critical for catalysis By similarity
	Site	181	1	Critical for catalysis By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q28480-1 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: 9100B663642131B9	FASTA	347	37,598
	10 20 30 40 50 60 QKQVTRGFTG GVQTVTLIPG DGIGPEISAA VMKIFDAAKA PIQWEERNVT AIQGPGGKWM 70 80 90 100 110 120 IPSEAKESMD KNKMGLKGPL KTPIAAGHPS MNLLLRKTFD LYANVRPCVS IEGYKTPYTD 130 140 150 160 170 180 VNIVTIRENT EGEYSGIEHV IVDGVVQSIK LITEGGSKRI AEFAFEYARN NHRSNVTAVH 190 200 210 220 230 240 KANIMRMSDG LFLQKCREVA ENCKDIKFNE MYLDTVCLNM VQDPSQFDVL VMPNLYGDIL 250 260 270 280 290 300 SDLCAGLIGG LGVTPSGNIG ANGVAIFESV HGTAPDIAGK DMANPTALLL SAVMMLRHMG 310 320 330 340 LFDHAARIEA ACFATIKDGK SLTKDLGGNA KCSDFTEEIC RRVKDLD

References

[1]	"Molecular cloning and deduced amino acid sequences of the alpha- and beta-subunits of mammalian NAD(+)-isocitrate dehydrogenase." Nichols B.J., Perry A.C.F., Hall L., Denton R.M. Biochem. J. 310:917-922(1995) [PubMed: 7575427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X87172 mRNA. Translation: CAA60637.1. Different initiation.
PIR	S58664.
3D structure databases
HSSP	HSSP built from PDB template 1ISO based on UniProtKB P08200.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q28480.
Family and domain databases
InterPro	IPR004434. IsoCit_DHase_NAD_mit. IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00175. mito_nad_idh. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDH3A_MACFA

Accession

Primary (citable) accession number: Q28480

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 15, 1998
Last modified:	November 25, 2008
This is version 51 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)