Reviewed, UniProtKB/Swiss-Prot Q28479 (IDH3B_MACFA)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
    EC=1.1.1.41
Alternative name(s):
    Isocitric dehydrogenase
    NAD(+)-specific ICDH

Gene names

Name:	IDH3B
ORF Names:	QnpA-21101

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subunit structure	Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1 By similarity.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 34	34	Mitochondrion By similarity
	Chain	35 – 385	351	Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial		PRO_0000014445
Regions
	Nucleotide binding	50 – 78	29	NAD Potential
Sites
	Metal binding	251	1	Magnesium or manganese By similarity
	Binding site	133	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	251	1	Substrate By similarity
	Site	171	1	Critical for catalysis By similarity
	Site	218	1	Critical for catalysis By similarity
Experimental info
	Sequence conflict	361	1	R → S in CAA57954. Ref.2
	Sequence conflict	367 – 385	19	STTTD…HPHGS → ATCHDFTEAVIAALPHP in CAA57954. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q28479-1 [UniParc]. Last modified May 30, 2006. Version 2. Checksum: 1730F0E614595EB3	FASTA	385	42,255
	10 20 30 40 50 60 MAALSGVRWL TRALVSAGNP GAWRGLSTSA AAHAASRSQA EDVRVEGSFP VTMLPGDGVG 70 80 90 100 110 120 PELMHAVKEV FKAAAVPVEF QEHHLSEVQN MASEEKLEQV LSSMKENKVA IIGKIHTPME 130 140 150 160 170 180 YKGELASYDM RLRRKLDLFA NVVHVKSLPG YMTRHNNLDL VIIREQTEGE YSSLEHESAR 190 200 210 220 230 240 GVIECLKIVT RAKSQRIAKF AFDYATKKGR SKVTAVHKAN IMKLGDGLFL QCCEEVAELY 250 260 270 280 290 300 PKIKFETMII DNCCMQLVQN PYQFDVLVMP NLYGNIIDNL AAGLVGGAGV VPGESYSAEY 310 320 330 340 350 360 AVFETGARHP FAQAVGRNIA NPTAMLLSAS NMLRHLNLEY HSNMIADAVK KVIKVGKVRT 370 380 RDMGGYSTTT DFIKSVIGHL HPHGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Parietal cortex.
[2]	"Molecular cloning and deduced amino acid sequences of the alpha- and beta-subunits of mammalian NAD(+)-isocitrate dehydrogenase." Nichols B.J., Perry A.C.F., Hall L., Denton R.M. Biochem. J. 310:917-922(1995) [PubMed: 7575427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-385. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB169839 mRNA. Translation: BAE01920.1. X82632 mRNA. Translation: CAA57954.1.
PIR	S58663.
3D structure databases
HSSP	HSSP built from PDB template 1HQS based on UniProtKB P39126.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q28479.
Enzyme and pathway databases
BRENDA	1.1.1.41. 3438.
Family and domain databases
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004434. Isocitrate_DH_NAD_mit. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00175. mito_nad_idh. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDH3B_MACFA

Accession

Primary (citable) accession number: Q28479
Secondary accession number(s): Q4R4Q5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 30, 2006
Last modified:	June 16, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)