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Q28479

- IDH3B_MACFA

UniProt

Q28479 - IDH3B_MACFA

Protein

Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial

Gene

IDH3B

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 2 (30 May 2006)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei133 – 1331SubstrateBy similarity
    Binding sitei164 – 1641SubstrateBy similarity
    Sitei171 – 1711Critical for catalysisBy similarity
    Sitei218 – 2181Critical for catalysisBy similarity
    Metal bindingi251 – 2511Magnesium or manganeseBy similarity
    Binding sitei251 – 2511SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi50 – 7829NADSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NAD+) activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial (EC:1.1.1.41)
    Alternative name(s):
    Isocitric dehydrogenase subunit beta
    NAD(+)-specific ICDH subunit beta
    Gene namesi
    Name:IDH3B
    ORF Names:QnpA-21101
    OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
    Taxonomic identifieri9541 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3434MitochondrionBy similarityAdd
    BLAST
    Chaini35 – 385351Isocitrate dehydrogenase [NAD] subunit beta, mitochondrialPRO_0000014445Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei199 – 1991N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiQ28479.

    Interactioni

    Subunit structurei

    Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ28479.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    HOVERGENiHBG052080.
    KOiK00030.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR004434. Isocitrate_DH_NAD.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11835. PTHR11835. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q28479-1 [UniParc]FASTAAdd to Basket

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    MAALSGVRWL TRALVSAGNP GAWRGLSTSA AAHAASRSQA EDVRVEGSFP    50
    VTMLPGDGVG PELMHAVKEV FKAAAVPVEF QEHHLSEVQN MASEEKLEQV 100
    LSSMKENKVA IIGKIHTPME YKGELASYDM RLRRKLDLFA NVVHVKSLPG 150
    YMTRHNNLDL VIIREQTEGE YSSLEHESAR GVIECLKIVT RAKSQRIAKF 200
    AFDYATKKGR SKVTAVHKAN IMKLGDGLFL QCCEEVAELY PKIKFETMII 250
    DNCCMQLVQN PYQFDVLVMP NLYGNIIDNL AAGLVGGAGV VPGESYSAEY 300
    AVFETGARHP FAQAVGRNIA NPTAMLLSAS NMLRHLNLEY HSNMIADAVK 350
    KVIKVGKVRT RDMGGYSTTT DFIKSVIGHL HPHGS 385
    Length:385
    Mass (Da):42,255
    Last modified:May 30, 2006 - v2
    Checksum:i1730F0E614595EB3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti361 – 3611R → S in CAA57954. (PubMed:7575427)Curated
    Sequence conflicti367 – 38519STTTD…HPHGS → ATCHDFTEAVIAALPHP in CAA57954. (PubMed:7575427)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB169839 mRNA. Translation: BAE01920.1.
    X82632 mRNA. Translation: CAA57954.1.
    PIRiS58663.
    RefSeqiNP_001274565.1. NM_001287636.1.
    XP_005568569.1. XM_005568512.1.
    UniGeneiMfa.1489.

    Genome annotation databases

    GeneIDi102129545.
    KEGGimcf:102129545.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB169839 mRNA. Translation: BAE01920.1 .
    X82632 mRNA. Translation: CAA57954.1 .
    PIRi S58663.
    RefSeqi NP_001274565.1. NM_001287636.1.
    XP_005568569.1. XM_005568512.1.
    UniGenei Mfa.1489.

    3D structure databases

    ProteinModelPortali Q28479.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q28479.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 102129545.
    KEGGi mcf:102129545.

    Organism-specific databases

    CTDi 3420.

    Phylogenomic databases

    HOVERGENi HBG052080.
    KOi K00030.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR001804. Isocitrate/isopropylmalate_DH.
    IPR004434. Isocitrate_DH_NAD.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11835. PTHR11835. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00175. mito_nad_idh. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
      International consortium for macaque cDNA sequencing and analysis
      Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Parietal cortex.
    2. "Molecular cloning and deduced amino acid sequences of the alpha- and beta-subunits of mammalian NAD(+)-isocitrate dehydrogenase."
      Nichols B.J., Perry A.C.F., Hall L., Denton R.M.
      Biochem. J. 310:917-922(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-385.
      Tissue: Testis.

    Entry informationi

    Entry nameiIDH3B_MACFA
    AccessioniPrimary (citable) accession number: Q28479
    Secondary accession number(s): Q4R4Q5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: May 30, 2006
    Last modified: October 1, 2014
    This is version 86 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3