Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q28478 (ADAM2_MACFA) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Disintegrin and metalloproteinase domain-containing protein 2
Short name=ADAM 2
Alternative name(s):
Fertilin subunit beta
PH-30
Short name=PH30
PH30-beta
Gene names
Name:ADAM2
Synonyms:FTNB
ORF Names:QtsA-17331
OrganismMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifier9541 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length735 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. Could have a direct role in sperm-zona binding or migration of sperm from the uterus into the oviduct. Interactions with egg membrane could be mediated via binding between its disintegrin-like domain to one or more integrins receptors on the egg. This is a non catalytic metalloprotease-like protein By similarity.

Subunit structure

Heterodimer with ADAM1/fertilin subunit alpha.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Expressed specifically in testis.

Domain

A tripeptide motif (FDE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding By similarity.

Post-translational modification

The signal and the metalloprotease domain are cleaved during the epididymal maturation of the spermatozoa By similarity.

Sequence similarities

Contains 1 disintegrin domain.

Contains 1 EGF-like domain.

Contains 1 peptidase M12B domain.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentMembrane
   DomainEGF-like domain
Signal
Transmembrane
Transmembrane helix
   PTMDisulfide bond
Glycoprotein
Gene Ontology (GO)
   Biological processcell adhesion

Inferred from electronic annotation. Source: UniProtKB-KW

proteolysis

Inferred from electronic annotation. Source: InterPro

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionmetalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616 Potential
Propeptide17 – 174158 By similarity
PRO_0000029044
Chain175 – 735561Disintegrin and metalloproteinase domain-containing protein 2
PRO_0000029045

Regions

Topological domain17 – 686670Extracellular Potential
Transmembrane687 – 70721Helical; Potential
Topological domain708 – 73528Cytoplasmic Potential
Domain178 – 375198Peptidase M12B
Domain384 – 47390Disintegrin
Domain612 – 64534EGF-like
Compositional bias476 – 606131Cys-rich

Amino acid modifications

Glycosylation761N-linked (GlcNAc...) Potential
Glycosylation1221N-linked (GlcNAc...) Potential
Glycosylation2201N-linked (GlcNAc...) Potential
Glycosylation3531N-linked (GlcNAc...) Potential
Glycosylation4591N-linked (GlcNAc...) Potential
Glycosylation5661N-linked (GlcNAc...) Potential
Disulfide bond287 ↔ 370 By similarity
Disulfide bond329 ↔ 354 By similarity
Disulfide bond331 ↔ 336 By similarity
Disulfide bond445 ↔ 465 By similarity
Disulfide bond616 ↔ 627 By similarity
Disulfide bond621 ↔ 633 By similarity
Disulfide bond635 ↔ 644 By similarity

Experimental info

Sequence conflict651F → S in BAE01208. Ref.3
Sequence conflict1211E → G in BAE01208. Ref.3
Sequence conflict2251L → P in BAE01208. Ref.3
Sequence conflict6811H → Y in BAE01208. Ref.3
Sequence conflict7231T → S in CAA54733. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q28478 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: BE84BE1158C60B53

FASTA73582,372
        10         20         30         40         50         60 
MWRVLFLLSG LGGLWMDSNF DSLPVQITVP EKIRSIIKEE IESQVSYKIV IEGKPYTANL 

        70         80         90        100        110        120 
MQKNFLSHNF RVYSYNGTGI MKPLDQDFQN FCHYQGYIEG YPKSVAMVST CTGLRGLLQF 

       130        140        150        160        170        180 
ENVSYGIEPL ESSVGFEHVI YQVKHKKADV SLYNEKDIES RDLSFKLQSI EPQKDFAKYI 

       190        200        210        220        230        240 
EMHVVVEKQL YNHMGSGTTV VTQKIFQLIG LTNAIFVSLN ITVILSSLEL WIDENKIATT 

       250        260        270        280        290        300 
GDAKELLHTF LRWKRSYLVL RPHDVAFLLV YREKSNYVGA TFQGKMCDAN YAGGVLLHPR 

       310        320        330        340        350        360 
TISLESLAVI LAQLLSLSMG IPYDDINQCQ CSAAVCIMNP EAIHFSGVKI FSNCSIEDFA 

       370        380        390        400        410        420 
HFISKQKSQC LHNQPRLDPF FKQQAVCGNA KLEAGEECDC GTQQNCFLLG AKCCDTATCR 

       430        440        450        460        470        480 
FKAGSNCAEG PCCENCLFMS QERVCRPSFD ECDLPEYCNG TSASCPENHF IQTGHPCGPN 

       490        500        510        520        530        540 
QWVCIDGVCM NGDKQCMDTF GGEAEFGPTE CYSYLNSKTD VSGNCGIGDS GYTQCEADNL 

       550        560        570        580        590        600 
QCGKLICKYA GEFLLQIPRA TIIYANISGH LCVAVEFASD HEDSHKMWIK DGTSCGSNKV 

       610        620        630        640        650        660 
CKNQRCVSSS YLGYDCTTDK CNHRGVCNNK KHCHCSASYL PPDCSVQSDT SPGGSIDSGN 

       670        680        690        700        710        720 
FPLVAVPARL PERRHMENVY HSKPMRWPLF LFIPFFIIFC VLIAIMVKVH FQRKKWRTED 

       730 
YSTDEQPESE SEPKG

« Hide

References

« Hide 'large scale' references
[1]"Cloning and analysis of monkey fertilin reveals novel alpha subunit isoforms."
Perry A.C.F., Gichuhi P.M., Jones R., Hall L.
Biochem. J. 307:843-850(1995) [PubMed: 7741716] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Testis.
[2]"Initial evaluation of fertilin as an immunocontraceptive antigen and molecular cloning of the cynomolgus monkey fertilin beta subunit."
Ramarao C.S., Myles D.G., White J.M., Primakoff P.
Mol. Reprod. Dev. 43:70-75(1996) [PubMed: 8720115] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications."
International consortium for macaque cDNA sequencing and analysis
Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X77653 mRNA. Translation: CAA54733.1.
U33959 mRNA. Translation: AAB52699.1.
AB169114 mRNA. Translation: BAE01208.1.
PIRG02937.

3D structure databases

ProteinModelPortalQ28478.
ModBaseSearch...

Protein family/group databases

MEROPSM12.950.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG103628.

Family and domain databases

InterProIPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR000742. EGF_3.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
Gene3DG3DSA:4.10.70.10. Blood-coag_inhib_Disintegrin. 1 hit.
G3DSA:3.40.390.10. G3DSA:3.40.390.10. 1 hit.
PfamPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSPR00289. DISINTEGRIN.
SMARTSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMSSF57552. Disintegrin. 1 hit.
PROSITEPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00022. EGF_1. False negative.
PS01186. EGF_2. False negative.
PS50026. EGF_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameADAM2_MACFA
AccessionPrimary (citable) accession number: Q28478
Secondary accession number(s): Q28472, Q4R6R6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: January 23, 2007
Last modified: September 21, 2011
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents