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Q28399 (ALDH1_ELEED) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aldehyde dehydrogenase, cytosolic 1

EC=1.2.1.3
Alternative name(s):
ALDH class 1
ETA-crystallin
Gene names
Name:ALDH1
OrganismElephantulus edwardii (Cape long-eared elephant shrew)
Taxonomic identifier28737 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaAfrotheriaMacroscelideaMacroscelididaeElephantulus

Protein attributes

Sequence length501 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Major component of the eye of elephant shrews, which in contrast to other mammals, possesses both a lens- and a non-lens class-1 aldehyde dehydrogenase 1. This eye-specific form is a structural protein of the lens and, in other part of the eye, serves as the major form of ALDH1. Can convert/oxidize retinaldehyde to retinoic acid. Ref.2

Catalytic activity

An aldehyde + NAD+ + H2O = a carboxylate + NADH.

Pathway

Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.

Subunit structure

Homotetramer. Ref.2

Subcellular location

Cytoplasm By similarity.

Tissue specificity

Eye specific, with very high expression in the lens.

Sequence similarities

Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processethanol catabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionaldehyde dehydrogenase (NAD) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 501501Aldehyde dehydrogenase, cytosolic 1
PRO_0000056427

Regions

Nucleotide binding246 – 2516NAD

Sites

Active site2691Proton acceptor
Active site3031Nucleophile
Site1701Transition state stabilizer

Secondary structure

................................................................................... 501
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q28399 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 9E3D5BBE083ACAD7

FASTA50154,538
        10         20         30         40         50         60 
MSSSGMPDLP APLTNIKIQH TKLFINNEWH ESVSGKTFPV FNPATEEKIC EVEEADKEDV 

        70         80         90        100        110        120 
DKAVKAAREA FQMGSPWRTM DASERGQLIY KLADLIERDR LLLATLESIN AGKVFASAYL 

       130        140        150        160        170        180 
MDLDYCIKAL RYCAGWADKI QGRTIPVDGE FFSYTRHEPI GVCGLIFPWN APMILLACKI 

       190        200        210        220        230        240 
GPALCCGNTV IVKPAEQTPL TALHVASLIK EAGFPPGVVN IVPGYGPTAG AAISSHMDVD 

       250        260        270        280        290        300 
KVAFTGSTEV GKMIQEAAAK SNLKRVTLEL GAKNPCIVFA DADLDSAVEF AHQGVFTNQG 

       310        320        330        340        350        360 
QSCIAASKLF VEEAIYDEFV QRSVERAKKY VFGNPLTPGV NHGPQINKAQ HNKIMELIES 

       370        380        390        400        410        420 
GKKEGAKLEC GGGPWGNKGY FIQPTVFSNV TDDMRIAKEE IFGPVQQIMK FKSLDEVIKR 

       430        440        450        460        470        480 
ANNTYYGLVA GVFTKDLDKA VTVSSALQAG TVWVNCYLAA SAQSPAGGFK MSGHGREMGE 

       490        500 
YGIHEYTEVK TVTMKISEKN S 

« Hide

References

[1]"A retinaldehyde dehydrogenase as a structural protein in a mammalian eye lens. Gene recruitment of eta-crystallin."
Graham C., Hodin J., Wistow G.
J. Biol. Chem. 271:15623-15628(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Lens.
[2]"Crystal structure of eta-crystallin: adaptation of a class 1 aldehyde dehydrogenase for a new role in the eye lens."
Bateman O.A., Purkiss A.G., van Montfort R., Slingsby C., Graham C., Wistow G.
Biochemistry 42:4349-4356(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) IN COMPLEX WITH NAD, FUNCTION, HOMOTETRAMERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U02483 mRNA. Translation: AAB60268.1.
RefSeqNP_001277088.1. NM_001290159.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1O9JX-ray2.40A/B/C/D1-501[»]
1PEJmodel-A1-501[»]
ProteinModelPortalQ28399.
SMRQ28399. Positions 8-501.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ28399.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID102867337.

Phylogenomic databases

HOVERGENHBG000097.

Enzyme and pathway databases

BRENDA1.2.1.36. 7360.
UniPathwayUPA00780; UER00768.

Family and domain databases

Gene3D3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMSSF53720. SSF53720. 1 hit.
PROSITEPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ28399.

Entry information

Entry nameALDH1_ELEED
AccessionPrimary (citable) accession number: Q28399
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways