SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q28399

- ALDH1_ELEED

UniProt

Q28399 - ALDH1_ELEED

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Aldehyde dehydrogenase, cytosolic 1

Gene
ALDH1
Organism
Elephantulus edwardii (Cape long-eared elephant shrew)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Major component of the eye of elephant shrews, which in contrast to other mammals, possesses both a lens- and a non-lens class-1 aldehyde dehydrogenase 1. This eye-specific form is a structural protein of the lens and, in other part of the eye, serves as the major form of ALDH1. Can convert/oxidize retinaldehyde to retinoic acid.1 Publication

Catalytic activityi

An aldehyde + NAD+ + H2O = a carboxylate + NADH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei170 – 1701Transition state stabilizer
Active sitei269 – 2691Proton acceptor
Active sitei303 – 3031Nucleophile

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi246 – 2516NAD

GO - Molecular functioni

  1. aldehyde dehydrogenase (NAD) activity Source: UniProtKB-EC

GO - Biological processi

  1. ethanol catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BRENDAi1.2.1.36. 7360.
UniPathwayiUPA00780; UER00768.

Names & Taxonomyi

Protein namesi
Recommended name:
Aldehyde dehydrogenase, cytosolic 1 (EC:1.2.1.3)
Alternative name(s):
ALDH class 1
ETA-crystallin
Gene namesi
Name:ALDH1
OrganismiElephantulus edwardii (Cape long-eared elephant shrew)
Taxonomic identifieri28737 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaAfrotheriaMacroscelideaMacroscelididaeElephantulus

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 501501Aldehyde dehydrogenase, cytosolic 1PRO_0000056427Add
BLAST

Proteomic databases

PRIDEiQ28399.

Expressioni

Tissue specificityi

Eye specific, with very high expression in the lens.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Structurei

Secondary structure

1
501
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi22 – 254
Beta strandi28 – 303
Beta strandi37 – 415
Turni43 – 453
Beta strandi48 – 536
Helixi57 – 7115
Helixi76 – 794
Helixi82 – 9817
Helixi100 – 11112
Helixi115 – 1206
Helixi122 – 13615
Helixi137 – 1393
Beta strandi142 – 1454
Beta strandi148 – 15912
Beta strandi162 – 1665
Helixi172 – 18514
Beta strandi189 – 1935
Helixi200 – 21213
Beta strandi218 – 2214
Turni226 – 2283
Helixi229 – 2346
Beta strandi241 – 2466
Helixi248 – 26013
Beta strandi265 – 2695
Beta strandi274 – 2785
Helixi284 – 29613
Helixi297 – 3004
Beta strandi306 – 3127
Helixi313 – 32614
Helixi348 – 36417
Beta strandi367 – 3704
Beta strandi373 – 3797
Beta strandi385 – 3906
Helixi395 – 3984
Beta strandi403 – 4119
Helixi414 – 4229
Beta strandi423 – 4253
Beta strandi428 – 4336
Helixi437 – 44610
Beta strandi449 – 4557
Helixi470 – 4723
Beta strandi473 – 4753
Helixi479 – 4835
Helixi484 – 4863
Beta strandi487 – 4959

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1O9JX-ray2.40A/B/C/D1-501[»]
1PEJmodel-A1-501[»]
ProteinModelPortaliQ28399.
SMRiQ28399. Positions 8-501.

Miscellaneous databases

EvolutionaryTraceiQ28399.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG000097.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q28399-1 [UniParc]FASTAAdd to Basket

« Hide

MSSSGMPDLP APLTNIKIQH TKLFINNEWH ESVSGKTFPV FNPATEEKIC    50
EVEEADKEDV DKAVKAAREA FQMGSPWRTM DASERGQLIY KLADLIERDR 100
LLLATLESIN AGKVFASAYL MDLDYCIKAL RYCAGWADKI QGRTIPVDGE 150
FFSYTRHEPI GVCGLIFPWN APMILLACKI GPALCCGNTV IVKPAEQTPL 200
TALHVASLIK EAGFPPGVVN IVPGYGPTAG AAISSHMDVD KVAFTGSTEV 250
GKMIQEAAAK SNLKRVTLEL GAKNPCIVFA DADLDSAVEF AHQGVFTNQG 300
QSCIAASKLF VEEAIYDEFV QRSVERAKKY VFGNPLTPGV NHGPQINKAQ 350
HNKIMELIES GKKEGAKLEC GGGPWGNKGY FIQPTVFSNV TDDMRIAKEE 400
IFGPVQQIMK FKSLDEVIKR ANNTYYGLVA GVFTKDLDKA VTVSSALQAG 450
TVWVNCYLAA SAQSPAGGFK MSGHGREMGE YGIHEYTEVK TVTMKISEKN 500
S 501
Length:501
Mass (Da):54,538
Last modified:November 1, 1996 - v1
Checksum:i9E3D5BBE083ACAD7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U02483 mRNA. Translation: AAB60268.1.
RefSeqiNP_001277088.1. NM_001290159.1.

Genome annotation databases

GeneIDi102867337.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U02483 mRNA. Translation: AAB60268.1 .
RefSeqi NP_001277088.1. NM_001290159.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1O9J X-ray 2.40 A/B/C/D 1-501 [» ]
1PEJ model - A 1-501 [» ]
ProteinModelPortali Q28399.
SMRi Q28399. Positions 8-501.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q28399.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 102867337.

Organism-specific databases

CTDi 102867337.

Phylogenomic databases

HOVERGENi HBG000097.

Enzyme and pathway databases

UniPathwayi UPA00780 ; UER00768 .
BRENDAi 1.2.1.36. 7360.

Miscellaneous databases

EvolutionaryTracei Q28399.

Family and domain databases

Gene3Di 3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProi IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view ]
Pfami PF00171. Aldedh. 1 hit.
[Graphical view ]
SUPFAMi SSF53720. SSF53720. 1 hit.
PROSITEi PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A retinaldehyde dehydrogenase as a structural protein in a mammalian eye lens. Gene recruitment of eta-crystallin."
    Graham C., Hodin J., Wistow G.
    J. Biol. Chem. 271:15623-15628(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lens.
  2. "Crystal structure of eta-crystallin: adaptation of a class 1 aldehyde dehydrogenase for a new role in the eye lens."
    Bateman O.A., Purkiss A.G., van Montfort R., Slingsby C., Graham C., Wistow G.
    Biochemistry 42:4349-4356(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) IN COMPLEX WITH NAD, FUNCTION, HOMOTETRAMERIZATION.

Entry informationi

Entry nameiALDH1_ELEED
AccessioniPrimary (citable) accession number: Q28399
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi