Reviewed, UniProtKB/Swiss-Prot Q28314 (NOS2_CAPHI)

Names and origin

Protein names

Recommended name:
    Nitric oxide synthase, inducible
    EC=1.14.13.39
Alternative name(s):
    Inducible NO synthase
      Short name=Inducible NOS
      Short name=iNOS
    NOS type II

Gene names

Name:

NOS2

Organism

Capra hircus (Goat)

Taxonomic identifier

9925 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Capra

Protein attributes

Sequence length	110 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body.
Catalytic activity	L-arginine + n NADPH + m O(2) = citrulline + nitric oxide + n NADP(+).
Cofactor	Heme group By similarity. Binds 1 FAD By similarity. Binds 1 FMN By similarity. Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.
Enzyme regulation	Regulated by calcium/calmodulin By similarity.
Subunit structure	Homodimer. Binds SLC9A3R1 By similarity.
Induction	By lipopolysaccharide (LPS).
Sequence similarities	Belongs to the NOS family.

Ontologies

Keywords
Ligand	Calcium Calmodulin-binding FAD FMN Heme Iron Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	nitric oxide biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitric-oxide synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›110	›110	Nitric oxide synthase, inducible		PRO_0000170928
Sites
	Metal binding	35	1	Iron (heme axial ligand) By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	110	1

Sequences

	Sequence		Length	Mass (Da)
	Q28314-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D72188FB257EC518	FASTA	110	12,654
	10 20 30 40 50 60 VEAVTKEIET TGTYQLTGDE LIFATKQAWR NAPRCIGRIQ WSNLQVFDAR SCSTAQEMFE 70 80 90 100 110 HICRHVRYAT NNGNIRSAIT VFPQRSDGKH DFRVWNAQLI RYAGYQMPDG

References

[1]

"Differential regulation of inducible nitric oxide synthase production in bovine and caprine macrophages." Adler H., Adler B., Peveri P., Werner E.R., Wachter H., Peterhans E., Jungi T.W. J. Infect. Dis. 173:971-978(1996) [PubMed: 8603979] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Peripheral blood monocyte.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U29085 mRNA. Translation: AAB02338.1.
3D structure databases
HSSP	HSSP built from PDB template 4NOS based on UniProtKB P35228.
SMR	Q28314. Positions 1-110.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q28314.
Family and domain databases
InterPro	IPR004030. NO_synthase_oxygenase_reg. [Graphical view]
Gene3D	G3DSA:3.90.340.10. NO_synthase_oxygenase_reg. 1 hit.
Pfam	PF02898. NO_synthase. 1 hit. [Graphical view]
PROSITE	PS60001. NOS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NOS2_CAPHI

Accession

Primary (citable) accession number: Q28314

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)