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Q28314

- NOS2_CAPHI

UniProt

Q28314 - NOS2_CAPHI

Protein

Nitric oxide synthase, inducible

Gene

NOS2

Organism
Capra hircus (Goat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
  1. Functioni

    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 By similarity.By similarity

    Catalytic activityi

    2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

    Cofactori

    Heme group.By similarity
    Binds 1 FAD.By similarity
    Binds 1 FMN.By similarity
    Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.By similarity

    Enzyme regulationi

    Regulated by calcium/calmodulin.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi35 – 351Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. nitric-oxide synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. nitric oxide biosynthetic process Source: InterPro
    2. peptidyl-cysteine S-nitrosylation Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Calcium, Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitric oxide synthase, inducible (EC:1.14.13.39)
    Alternative name(s):
    Inducible NO synthase
    Short name:
    Inducible NOS
    Short name:
    iNOS
    NOS type II
    Peptidyl-cysteine S-nitrosylase NOS2
    Gene namesi
    Name:NOS2
    OrganismiCapra hircus (Goat)
    Taxonomic identifieri9925 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeCapra

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – ›110›110Nitric oxide synthase, induciblePRO_0000170928Add
    BLAST

    Expressioni

    Inductioni

    By lipopolysaccharide (LPS).

    Interactioni

    Subunit structurei

    Homodimer. Binds SLC9A3R1 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ28314.
    SMRiQ28314. Positions 1-110.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NOS family.Curated

    Family and domain databases

    Gene3Di3.90.340.10. 1 hit.
    InterProiIPR004030. NOS_N.
    [Graphical view]
    PfamiPF02898. NO_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56512. SSF56512. 1 hit.
    PROSITEiPS60001. NOS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    Q28314-1 [UniParc]FASTAAdd to Basket

    « Hide

    VEAVTKEIET TGTYQLTGDE LIFATKQAWR NAPRCIGRIQ WSNLQVFDAR    50
    SCSTAQEMFE HICRHVRYAT NNGNIRSAIT VFPQRSDGKH DFRVWNAQLI 100
    RYAGYQMPDG 110
    Length:110
    Mass (Da):12,654
    Last modified:November 1, 1996 - v1
    Checksum:iD72188FB257EC518
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11
    Non-terminal residuei110 – 1101

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U29085 mRNA. Translation: AAB02338.1.
    UniGeneiChi.38774.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U29085 mRNA. Translation: AAB02338.1 .
    UniGenei Chi.38774.

    3D structure databases

    ProteinModelPortali Q28314.
    SMRi Q28314. Positions 1-110.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.90.340.10. 1 hit.
    InterProi IPR004030. NOS_N.
    [Graphical view ]
    Pfami PF02898. NO_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56512. SSF56512. 1 hit.
    PROSITEi PS60001. NOS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Differential regulation of inducible nitric oxide synthase production in bovine and caprine macrophages."
      Adler H., Adler B., Peveri P., Werner E.R., Wachter H., Peterhans E., Jungi T.W.
      J. Infect. Dis. 173:971-978(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Peripheral blood monocyte.

    Entry informationi

    Entry nameiNOS2_CAPHI
    AccessioniPrimary (citable) accession number: Q28314
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3