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Q28314 (NOS2_CAPHI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitric oxide synthase, inducible

EC=1.14.13.39
Alternative name(s):
Inducible NO synthase
Short name=Inducible NOS
Short name=iNOS
NOS type II
Peptidyl-cysteine S-nitrosylase NOS2
Gene names
Name:NOS2
OrganismCapra hircus (Goat)
Taxonomic identifier9925 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeCapra

Protein attributes

Sequence length110 AA.
Sequence statusFragment.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 By similarity.

Catalytic activity

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor

Heme group By similarity.

Binds 1 FAD By similarity.

Binds 1 FMN By similarity.

Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.

Enzyme regulation

Regulated by calcium/calmodulin By similarity.

Subunit structure

Homodimer. Binds SLC9A3R1 By similarity.

Induction

By lipopolysaccharide (LPS).

Sequence similarities

Belongs to the NOS family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – ›110›110Nitric oxide synthase, inducible
PRO_0000170928

Sites

Metal binding351Iron (heme axial ligand) By similarity

Experimental info

Non-terminal residue11
Non-terminal residue1101

Sequences

Sequence LengthMass (Da)Tools
Q28314 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: D72188FB257EC518

FASTA11012,654
        10         20         30         40         50         60 
VEAVTKEIET TGTYQLTGDE LIFATKQAWR NAPRCIGRIQ WSNLQVFDAR SCSTAQEMFE 

        70         80         90        100        110 
HICRHVRYAT NNGNIRSAIT VFPQRSDGKH DFRVWNAQLI RYAGYQMPDG 

« Hide

References

[1]"Differential regulation of inducible nitric oxide synthase production in bovine and caprine macrophages."
Adler H., Adler B., Peveri P., Werner E.R., Wachter H., Peterhans E., Jungi T.W.
J. Infect. Dis. 173:971-978(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Peripheral blood monocyte.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U29085 mRNA. Translation: AAB02338.1.
UniGeneChi.38774.

3D structure databases

ProteinModelPortalQ28314.
SMRQ28314. Positions 1-110.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.90.340.10. 1 hit.
InterProIPR004030. NO_synthase_oxygenase_dom.
[Graphical view]
PfamPF02898. NO_synthase. 1 hit.
[Graphical view]
SUPFAMSSF56512. SSF56512. 1 hit.
PROSITEPS60001. NOS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNOS2_CAPHI
AccessionPrimary (citable) accession number: Q28314
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: January 22, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families