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Protein

Hematopoietic progenitor cell antigen CD34

Gene

CD34

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Hematopoietic progenitor cell antigen CD34
Alternative name(s):
CD_antigen: CD34
Gene namesi
Name:CD34
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini34 – 294261ExtracellularSequence analysisAdd
BLAST
Transmembranei295 – 31521HelicalSequence analysisAdd
BLAST
Topological domaini316 – 38974CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 33331 PublicationAdd
BLAST
Chaini34 – 389356Hematopoietic progenitor cell antigen CD34PRO_0000020899Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi55 – 551N-linked (GlcNAc...)Sequence analysis
Glycosylationi107 – 1071N-linked (GlcNAc...)Sequence analysis
Glycosylationi126 – 1261N-linked (GlcNAc...)Sequence analysis
Glycosylationi142 – 1421N-linked (GlcNAc...)Sequence analysis
Glycosylationi149 – 1491N-linked (GlcNAc...)Sequence analysis
Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence analysis
Glycosylationi253 – 2531N-linked (GlcNAc...)Sequence analysis
Modified residuei333 – 3331PhosphotyrosineBy similarity
Modified residuei343 – 3431PhosphotyrosineBy similarity
Modified residuei350 – 3501PhosphoserineBy similarity

Post-translational modificationi

Highly glycosylated.By similarity
Phosphorylated on serine residues by PKC.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ28270.

Expressioni

Tissue specificityi

Selectively expressed on hematopoietic progenitor cells and vascular endothelium.

Developmental stagei

On early hematopoietic progenitor cells.

Interactioni

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000017250.

Family & Domainsi

Sequence similaritiesi

Belongs to the CD34 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IPJJ. Eukaryota.
ENOG410YPR6. LUCA.
HOGENOMiHOG000052513.
HOVERGENiHBG003461.
InParanoidiQ28270.
KOiK06474.

Family and domain databases

InterProiIPR008083. CD34.
IPR013836. CD34/Podocalyxin.
[Graphical view]
PANTHERiPTHR16677. PTHR16677. 1 hit.
PfamiPF06365. CD34_antigen. 1 hit.
[Graphical view]
PRINTSiPR01700. CD34ANTIGEN.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q28270-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLAGRGARAG GGLPRGWTAL CLLSLLPFGF TNTETVITPT TVPTSTEIMS
60 70 80 90 100
AVSENTSKRE AITLTPSGTT TLYSVSQDSS GTTATISETT VHVTSTSEIT
110 120 130 140 150
LTPGTMNSSV QSQTSLAITV SFTPTNFSTS SVTLEPSLLP GNGSDPPYNS
160 170 180 190 200
TSLVTSPTEY YTSLSPTPSR NDTPSTIKGE IKCSGVKEVK LNQGICLELN
210 220 230 240 250
ETSSCEDFKK DNEEKLTQVL CEKEPAEAGA GVCSLLLAQS EVRPHCLLLV
260 270 280 290 300
LANKTELFSK LQLLRKHQSD LKKLGIRDFT EQDVGSHQSY SRKTLIALVT
310 320 330 340 350
SGILLAVLGT TGYFLMNRRS WSPTGERLGE DPYYTENGGG QGYSSGPGVS
360 370 380
PEAQGKASVN RGPQENGTGQ ATSRNGHSAR QHMVADTEL
Length:389
Mass (Da):41,285
Last modified:November 1, 1996 - v1
Checksum:i1E3D1198720A3CF8
GO
Isoform Short (identifier: Q28270-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     329-332: GEDP → ELEP
     333-389: Missing.

Show »
Length:332
Mass (Da):35,523
Checksum:iAAA290EE69485AB2
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei329 – 3324GEDP → ELEP in isoform Short. 1 PublicationVSP_004157
Alternative sequencei333 – 38957Missing in isoform Short. 1 PublicationVSP_004158Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49457 mRNA. Translation: AAB41055.1.
U49458 mRNA. Translation: AAB48004.1.
RefSeqiNP_001003341.1. NM_001003341.1. [Q28270-1]
UniGeneiCfa.3771.

Genome annotation databases

GeneIDi415130.
KEGGicfa:415130.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49457 mRNA. Translation: AAB41055.1.
U49458 mRNA. Translation: AAB48004.1.
RefSeqiNP_001003341.1. NM_001003341.1. [Q28270-1]
UniGeneiCfa.3771.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000017250.

Proteomic databases

PaxDbiQ28270.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi415130.
KEGGicfa:415130.

Organism-specific databases

CTDi947.

Phylogenomic databases

eggNOGiENOG410IPJJ. Eukaryota.
ENOG410YPR6. LUCA.
HOGENOMiHOG000052513.
HOVERGENiHBG003461.
InParanoidiQ28270.
KOiK06474.

Miscellaneous databases

NextBioi20818809.

Family and domain databases

InterProiIPR008083. CD34.
IPR013836. CD34/Podocalyxin.
[Graphical view]
PANTHERiPTHR16677. PTHR16677. 1 hit.
PfamiPF06365. CD34_antigen. 1 hit.
[Graphical view]
PRINTSiPR01700. CD34ANTIGEN.
ProtoNetiSearch...

Publicationsi

  1. "Canine CD34: cloning of the cDNA and evaluation of an antiserum to recombinant protein."
    McSweeney P.A., Rouleau K.A., Storb R., Bolles L., Wallace P.M., Beauchamp M., Krizanac-Bengez L., Moore P., Sale G., Sandmaier B., de Revel T., Appelbaum F.R., Nash R.A.
    Blood 88:1992-2003(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT), PROTEIN SEQUENCE OF 34-52.
    Tissue: Myeloid leukemia cell.

Entry informationi

Entry nameiCD34_CANLF
AccessioniPrimary (citable) accession number: Q28270
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: December 9, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.