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Q28263

- PDE6A_CANFA

UniProt

Q28263 - PDE6A_CANFA

Protein

Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha

Gene

PDE6A

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    This protein participates in processes of transmission and amplification of the visual signal.

    Catalytic activityi

    Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

    Cofactori

    Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei559 – 5591Proton donorBy similarity
    Metal bindingi563 – 5631Divalent metal cation 1By similarity
    Metal bindingi599 – 5991Divalent metal cation 1By similarity
    Metal bindingi600 – 6001Divalent metal cation 1By similarity
    Metal bindingi600 – 6001Divalent metal cation 2By similarity
    Metal bindingi720 – 7201Divalent metal cation 1By similarity

    GO - Molecular functioni

    1. 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cytosolic calcium ion homeostasis Source: Ensembl
    2. GMP metabolic process Source: Ensembl
    3. retina development in camera-type eye Source: Ensembl
    4. signal transduction Source: InterPro
    5. visual perception Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Sensory transduction, Vision

    Keywords - Ligandi

    cGMP, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_174228. Activation of the phototransduction cascade.
    REACT_174305. Inactivation, recovery and regulation of the phototransduction cascade.
    REACT_175273. Ca2+ pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha (EC:3.1.4.35)
    Short name:
    GMP-PDE alpha
    Gene namesi
    Name:PDE6A
    Synonyms:PDEA
    OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
    Taxonomic identifieri9615 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
    ProteomesiUP000002254: Unplaced

    Subcellular locationi

    Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 858857Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alphaPRO_0000198827Add
    BLAST
    Propeptidei859 – 8613Removed in mature formBy similarityPRO_0000396696

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylglycineBy similarity
    Modified residuei858 – 8581Cysteine methyl esterBy similarity
    Lipidationi858 – 8581S-farnesyl cysteineBy similarity

    Keywords - PTMi

    Acetylation, Lipoprotein, Methylation, Prenylation

    Proteomic databases

    PaxDbiQ28263.

    Interactioni

    Subunit structurei

    Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.

    Protein-protein interaction databases

    STRINGi9615.ENSCAFP00000011716.

    Structurei

    3D structure databases

    ProteinModelPortaliQ28263.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini73 – 222150GAF 1Add
    BLAST
    Domaini254 – 431178GAF 2Add
    BLAST

    Sequence similaritiesi

    Contains 2 GAF domains.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG242608.
    HOGENOMiHOG000007069.
    HOVERGENiHBG053539.
    KOiK08718.

    Family and domain databases

    Gene3Di1.10.1300.10. 1 hit.
    3.30.450.40. 3 hits.
    InterProiIPR003018. GAF.
    IPR029016. GAF_dom_like.
    IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view]
    PfamiPF01590. GAF. 2 hits.
    PF00233. PDEase_I. 1 hit.
    [Graphical view]
    PRINTSiPR00387. PDIESTERASE1.
    SMARTiSM00065. GAF. 2 hits.
    SM00471. HDc. 1 hit.
    [Graphical view]
    SUPFAMiSSF55781. SSF55781. 3 hits.
    PROSITEiPS00126. PDEASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q28263-1 [UniParc]FASTAAdd to Basket

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    MGEVTAEQVE KFLDSNIIFA KQYYNLRYRA KVISDMLGAK EAAVDFSNYH    50
    SLSSVEESEI IFDLLRDFQE NLQAERCIFN VMKKLCFLLQ ADRMSLFMYR 100
    VRNGIAELAT RLFNVHKDAV LEECLVAPDS EIVFPLDMGV VGHVAHSKKI 150
    ANVVNTEEDE HFCDFVDTLT EYQTKNILAS PIMNGKDVVA VIMAVNKVDE 200
    PHFTKRDEEI LLKYLNFANL IMKVYHLSYL HNCETRRGQI LLWSGSKVFE 250
    ELTDIERQFH KALYTVRAFL NCDRYSVGLL DMTKQKEFFD VWPVLMGEAP 300
    PYSGPRTPDG REINFYKVID YILHGKEDIK VIPNPPPDHW ALVSGLPTYV 350
    AQNGLICNIM NAPAEDFFAF QKEPLDESGW MIKNVLSMPI VNKKEEIVGV 400
    ATFYNRKDGK PFDEMDETLM ESLAQFLGWS VLNPDTYESM NRLENRKDIF 450
    QDMVKYHVKC DNEEIQKILK TREVYGKEPW ECEEEELAEI LQGELPDAEK 500
    YEINKFHFSD LPLTELELVK CGIQMYYELK VVDKFHIPQE ALVRFMYSLS 550
    KGYRRITYHN WRHGFNVGQT MFSLLVTGKL KRYFTDLEAL AMVTAAFCHD 600
    IDHRGTNNLY QMKSQNPLAK LHGSSILERH HLEFGKTLLR DESLNIFQNL 650
    NRRQHEHAIH MMDIAIIATD LALYFKKRTM FQKIVDQSKT YETQQEWTQY 700
    MMLEQTRKEI VMAMMMTACD LSAITKPWEV QSKVALLVAA EFWEQGDLER 750
    TVLQQNPIPM MDRNKADELP KLQVGFIDFV CTFVYKEFSR FHEEITPMLD 800
    GITNNRKEWK ALADEYDTKM KALEEEKQKQ QTAKQGAAGD QPGGNPSPAG 850
    GAPASKSCCI Q 861
    Length:861
    Mass (Da):99,688
    Last modified:January 23, 2007 - v2
    Checksum:i8F7DD6C2A891B4E7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti388 – 3881M → L in AAB70037. (PubMed:9233984)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z68340 mRNA. Translation: CAA92763.1.
    U52868 mRNA. Translation: AAB70037.1.
    Y13282 mRNA. Translation: CAA73731.1.
    RefSeqiNP_001003073.1. NM_001003073.1.
    UniGeneiCfa.1198.

    Genome annotation databases

    GeneIDi403620.
    KEGGicfa:403620.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z68340 mRNA. Translation: CAA92763.1 .
    U52868 mRNA. Translation: AAB70037.1 .
    Y13282 mRNA. Translation: CAA73731.1 .
    RefSeqi NP_001003073.1. NM_001003073.1.
    UniGenei Cfa.1198.

    3D structure databases

    ProteinModelPortali Q28263.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9615.ENSCAFP00000011716.

    Chemistry

    BindingDBi Q28263.
    ChEMBLi CHEMBL5151.

    Proteomic databases

    PaxDbi Q28263.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 403620.
    KEGGi cfa:403620.

    Organism-specific databases

    CTDi 5145.

    Phylogenomic databases

    eggNOGi NOG242608.
    HOGENOMi HOG000007069.
    HOVERGENi HBG053539.
    KOi K08718.

    Enzyme and pathway databases

    Reactomei REACT_174228. Activation of the phototransduction cascade.
    REACT_174305. Inactivation, recovery and regulation of the phototransduction cascade.
    REACT_175273. Ca2+ pathway.

    Miscellaneous databases

    NextBioi 20817125.

    Family and domain databases

    Gene3Di 1.10.1300.10. 1 hit.
    3.30.450.40. 3 hits.
    InterProi IPR003018. GAF.
    IPR029016. GAF_dom_like.
    IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view ]
    Pfami PF01590. GAF. 2 hits.
    PF00233. PDEase_I. 1 hit.
    [Graphical view ]
    PRINTSi PR00387. PDIESTERASE1.
    SMARTi SM00065. GAF. 2 hits.
    SM00471. HDc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55781. SSF55781. 3 hits.
    PROSITEi PS00126. PDEASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Elevation of cGMP with normal expression and activity of rod cGMP-PDE in photoreceptor degenerate labrador retrievers."
      Kommonen B., Kylma T., Cohen R.J., Penn J.S., Paulin L., Hurwitz M., Hurwitz R.L.
      Ophthalmic Res. 28:19-28(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Retina.
    2. "Cloning and characterization of the cDNA encoding the alpha-subunit of cGMP-phosphodiesterase in canine retinal rod photoreceptor cells."
      Wang W., Acland G.M., Aguirre G.D., Ray K.
      Mol. Vis. 2:3-3(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. Veske A., Nilsson S.E.G., Gal A.
      Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Beagle X Briard.
      Tissue: Retina.

    Entry informationi

    Entry nameiPDE6A_CANFA
    AccessioniPrimary (citable) accession number: Q28263
    Secondary accession number(s): Q29470
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 94 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3