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Q28262

- PAFA_CANFA

UniProt

Q28262 - PAFA_CANFA

Protein

Platelet-activating factor acetylhydrolase

Gene

PLA2G7

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.

    Catalytic activityi

    1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei274 – 2741NucleophileBy similarity
    Active sitei297 – 2971Charge relay systemPROSITE-ProRule annotation
    Active sitei352 – 3521Charge relay systemPROSITE-ProRule annotation

    GO - Molecular functioni

    1. 1-alkyl-2-acetylglycerophosphocholine esterase activity Source: UniProtKB-EC

    GO - Biological processi

    1. lipid catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Platelet-activating factor acetylhydrolase (EC:3.1.1.47)
    Short name:
    PAF acetylhydrolase
    Alternative name(s):
    1-alkyl-2-acetylglycerophosphocholine esterase
    2-acetyl-1-alkylglycerophosphocholine esterase
    LDL-associated phospholipase A2
    Short name:
    LDL-PLA(2)
    PAF 2-acylhydrolase
    Gene namesi
    Name:PLA2G7
    OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
    Taxonomic identifieri9615 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
    ProteomesiUP000002254: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121By similarityAdd
    BLAST
    Chaini22 – 444423Platelet-activating factor acetylhydrolasePRO_0000017831Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi60 – 601N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi424 – 4241N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Tissue specificityi

    Plasma.

    Interactioni

    Protein-protein interaction databases

    STRINGi9615.ENSCAFP00000030617.

    Structurei

    3D structure databases

    ProteinModelPortaliQ28262.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AB hydrolase superfamily. Lipase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG4188.
    HOGENOMiHOG000008053.
    HOVERGENiHBG001322.
    InParanoidiQ28262.
    KOiK01062.

    Family and domain databases

    Gene3Di3.40.50.1820. 3 hits.
    InterProiIPR029058. AB_hydrolase.
    IPR005065. PAF_acetylhydro.
    IPR016715. PAF_acetylhydro_eukaryote.
    [Graphical view]
    PANTHERiPTHR10272. PTHR10272. 1 hit.
    PfamiPF03403. PAF-AH_p_II. 1 hit.
    [Graphical view]
    PIRSFiPIRSF018169. PAF_acetylhydrolase. 1 hit.
    SUPFAMiSSF53474. SSF53474. 3 hits.
    PROSITEiPS00120. LIPASE_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q28262-1 [UniParc]FASTAAdd to Basket

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    MLPPKLHALF CLCSCLTLVH PIDWQDLNPV AHIRSSAWAN KIQALMAAAS    50
    IRQSRIPKGN GSYSVGCTDL MFDYTNKGTF LRLYYPSQED DHSDTLWIPN 100
    KEYFFGLSKY LGTPWLMGKI LSFFFGSVTT PANWNSPLRT GEKYPLIVFS 150
    HGLGAFRTIY SAIGIDLASH GFIVAAIEHR DGSASATYYF KDQSAAEIGN 200
    KSWSYLQELK PGDEEIHVRN EQVQKRAKEC SQALNLILDI DHGRPIKNVL 250
    DLEFDVEQLK DSIDRDKIAV IGHSFGGATV LQALSEDQRF RCGIALDAWM 300
    LPLDDAIYSR IPQPLFFINS ERFQFPENIK KMKKCYSPDK ERKMITIRGS 350
    VHQNFADFTF TTGKIVGYIF TLKGDIDSNV AIDLCNKASL AFLQKHLGLR 400
    KDFDQWDSLI EGKDENLMPG TNINITNEHD TLQNSPEAEK SNLD 444
    Length:444
    Mass (Da):50,136
    Last modified:November 1, 1997 - v1
    Checksum:i814EF0AE38B074AC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U34246 mRNA. Translation: AAC48484.1.
    RefSeqiNP_001003198.1. NM_001003198.1.
    UniGeneiCfa.3709.

    Genome annotation databases

    GeneIDi403848.
    KEGGicfa:403848.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U34246 mRNA. Translation: AAC48484.1 .
    RefSeqi NP_001003198.1. NM_001003198.1.
    UniGenei Cfa.3709.

    3D structure databases

    ProteinModelPortali Q28262.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9615.ENSCAFP00000030617.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 403848.
    KEGGi cfa:403848.

    Organism-specific databases

    CTDi 7941.

    Phylogenomic databases

    eggNOGi COG4188.
    HOGENOMi HOG000008053.
    HOVERGENi HBG001322.
    InParanoidi Q28262.
    KOi K01062.

    Miscellaneous databases

    NextBioi 20817342.

    Family and domain databases

    Gene3Di 3.40.50.1820. 3 hits.
    InterProi IPR029058. AB_hydrolase.
    IPR005065. PAF_acetylhydro.
    IPR016715. PAF_acetylhydro_eukaryote.
    [Graphical view ]
    PANTHERi PTHR10272. PTHR10272. 1 hit.
    Pfami PF03403. PAF-AH_p_II. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF018169. PAF_acetylhydrolase. 1 hit.
    SUPFAMi SSF53474. SSF53474. 3 hits.
    PROSITEi PS00120. LIPASE_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Plasma platelet-activating factor acetylhydrolase is a secreted phospholipase A2 with a catalytic triad."
      Tjoelker L.W., Eberhardt C., Unger J., le Trong H., Zimmerman G.A., McIntyre T.M., Stafforini D.M., Prescott S.M., Gray P.W.
      J. Biol. Chem. 270:25481-25487(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Spleen.

    Entry informationi

    Entry nameiPAFA_CANFA
    AccessioniPrimary (citable) accession number: Q28262
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3