Q28262 (PAFA_CANFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Platelet-activating factor acetylhydrolase
Short name=PAF acetylhydrolase
EC=3.1.1.47

Alternative name(s):
1-alkyl-2-acetylglycerophosphocholine esterase
2-acetyl-1-alkylglycerophosphocholine esterase
LDL-associated phospholipase A2
Short name=LDL-PLA(2)
PAF 2-acylhydrolase

Gene names

Name:

PLA2G7

Organism

Canis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › Canis lupus

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.
Catalytic activity	1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.
Subcellular location	Secreted › extracellular space.
Tissue specificity	Plasma.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	1-alkyl-2-acetylglycerophosphocholine esterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	By similarity
	Chain	22 – 444	423	Platelet-activating factor acetylhydrolase		PRO_0000017831
Sites
	Active site	274	1	Nucleophile By similarity
	Active site	297	1	Charge relay system By similarity
	Active site	352	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	60	1	N-linked (GlcNAc...) Potential
	Glycosylation	200	1	N-linked (GlcNAc...) Potential
	Glycosylation	424	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q28262 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 814EF0AE38B074AC	FASTA	444	50,136
	10 20 30 40 50 60 MLPPKLHALF CLCSCLTLVH PIDWQDLNPV AHIRSSAWAN KIQALMAAAS IRQSRIPKGN 70 80 90 100 110 120 GSYSVGCTDL MFDYTNKGTF LRLYYPSQED DHSDTLWIPN KEYFFGLSKY LGTPWLMGKI 130 140 150 160 170 180 LSFFFGSVTT PANWNSPLRT GEKYPLIVFS HGLGAFRTIY SAIGIDLASH GFIVAAIEHR 190 200 210 220 230 240 DGSASATYYF KDQSAAEIGN KSWSYLQELK PGDEEIHVRN EQVQKRAKEC SQALNLILDI 250 260 270 280 290 300 DHGRPIKNVL DLEFDVEQLK DSIDRDKIAV IGHSFGGATV LQALSEDQRF RCGIALDAWM 310 320 330 340 350 360 LPLDDAIYSR IPQPLFFINS ERFQFPENIK KMKKCYSPDK ERKMITIRGS VHQNFADFTF 370 380 390 400 410 420 TTGKIVGYIF TLKGDIDSNV AIDLCNKASL AFLQKHLGLR KDFDQWDSLI EGKDENLMPG 430 440 TNINITNEHD TLQNSPEAEK SNLD

References

[1]

"Plasma platelet-activating factor acetylhydrolase is a secreted phospholipase A2 with a catalytic triad." Tjoelker L.W., Eberhardt C., Unger J., le Trong H., Zimmerman G.A., McIntyre T.M., Stafforini D.M., Prescott S.M., Gray P.W. J. Biol. Chem. 270:25481-25487(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Spleen.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U34246 mRNA. Translation: AAC48484.1.
RefSeq	NP_001003198.1. NM_001003198.1.
UniGene	Cfa.3709.
3D structure databases
ProteinModelPortal	Q28262.
ModBase	Search...
Protein-protein interaction databases
STRING	9615.ENSCAFP00000030617.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	403848.
KEGG	cfa:403848.
Organism-specific databases
CTD	7941.
Phylogenomic databases
eggNOG	COG4188.
HOGENOM	HOG000008053.
HOVERGEN	HBG001322.
InParanoid	Q28262.
KO	K01062.
OrthoDB	EOG4QC156.
Family and domain databases
InterPro	IPR016715. Ac_Ohase_PAF. IPR005065. PAF_acetylhydro. [Graphical view]
PANTHER	PTHR10272. PTHR10272. 1 hit.
Pfam	PF03403. PAF-AH_p_II. 1 hit. [Graphical view]
PIRSF	PIRSF018169. PAF_acetylhydrolase. 1 hit.
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20817342.

Entry information

Entry name

PAFA_CANFA

Accession

Primary (citable) accession number: Q28262

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program