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Protein

Platelet-activating factor acetylhydrolase

Gene

PLA2G7

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.

Catalytic activityi

1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei274 – 2741NucleophileBy similarity
Active sitei297 – 2971Charge relay systemPROSITE-ProRule annotation
Active sitei352 – 3521Charge relay systemPROSITE-ProRule annotation

GO - Molecular functioni

  1. 1-alkyl-2-acetylglycerophosphocholine esterase activity Source: UniProtKB-EC

GO - Biological processi

  1. lipid catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet-activating factor acetylhydrolase (EC:3.1.1.47)
Short name:
PAF acetylhydrolase
Alternative name(s):
1-alkyl-2-acetylglycerophosphocholine esterase
2-acetyl-1-alkylglycerophosphocholine esterase
LDL-associated phospholipase A2
Short name:
LDL-PLA(2)
PAF 2-acylhydrolase
Gene namesi
Name:PLA2G7
OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
ProteomesiUP000002254 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121By similarityAdd
BLAST
Chaini22 – 444423Platelet-activating factor acetylhydrolasePRO_0000017831Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi60 – 601N-linked (GlcNAc...)Sequence Analysis
Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence Analysis
Glycosylationi424 – 4241N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Plasma.

Interactioni

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000030617.

Structurei

3D structure databases

ProteinModelPortaliQ28262.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Lipase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG4188.
HOGENOMiHOG000008053.
HOVERGENiHBG001322.
InParanoidiQ28262.
KOiK01062.

Family and domain databases

Gene3Di3.40.50.1820. 3 hits.
InterProiIPR029058. AB_hydrolase.
IPR005065. PAF_acetylhydro.
IPR016715. PAF_acetylhydro_eukaryote.
[Graphical view]
PANTHERiPTHR10272. PTHR10272. 1 hit.
PfamiPF03403. PAF-AH_p_II. 1 hit.
[Graphical view]
PIRSFiPIRSF018169. PAF_acetylhydrolase. 1 hit.
SUPFAMiSSF53474. SSF53474. 3 hits.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q28262-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPPKLHALF CLCSCLTLVH PIDWQDLNPV AHIRSSAWAN KIQALMAAAS
60 70 80 90 100
IRQSRIPKGN GSYSVGCTDL MFDYTNKGTF LRLYYPSQED DHSDTLWIPN
110 120 130 140 150
KEYFFGLSKY LGTPWLMGKI LSFFFGSVTT PANWNSPLRT GEKYPLIVFS
160 170 180 190 200
HGLGAFRTIY SAIGIDLASH GFIVAAIEHR DGSASATYYF KDQSAAEIGN
210 220 230 240 250
KSWSYLQELK PGDEEIHVRN EQVQKRAKEC SQALNLILDI DHGRPIKNVL
260 270 280 290 300
DLEFDVEQLK DSIDRDKIAV IGHSFGGATV LQALSEDQRF RCGIALDAWM
310 320 330 340 350
LPLDDAIYSR IPQPLFFINS ERFQFPENIK KMKKCYSPDK ERKMITIRGS
360 370 380 390 400
VHQNFADFTF TTGKIVGYIF TLKGDIDSNV AIDLCNKASL AFLQKHLGLR
410 420 430 440
KDFDQWDSLI EGKDENLMPG TNINITNEHD TLQNSPEAEK SNLD
Length:444
Mass (Da):50,136
Last modified:October 31, 1997 - v1
Checksum:i814EF0AE38B074AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34246 mRNA. Translation: AAC48484.1.
RefSeqiNP_001003198.1. NM_001003198.1.
UniGeneiCfa.3709.

Genome annotation databases

GeneIDi403848.
KEGGicfa:403848.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34246 mRNA. Translation: AAC48484.1.
RefSeqiNP_001003198.1. NM_001003198.1.
UniGeneiCfa.3709.

3D structure databases

ProteinModelPortaliQ28262.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000030617.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi403848.
KEGGicfa:403848.

Organism-specific databases

CTDi7941.

Phylogenomic databases

eggNOGiCOG4188.
HOGENOMiHOG000008053.
HOVERGENiHBG001322.
InParanoidiQ28262.
KOiK01062.

Miscellaneous databases

NextBioi20817342.

Family and domain databases

Gene3Di3.40.50.1820. 3 hits.
InterProiIPR029058. AB_hydrolase.
IPR005065. PAF_acetylhydro.
IPR016715. PAF_acetylhydro_eukaryote.
[Graphical view]
PANTHERiPTHR10272. PTHR10272. 1 hit.
PfamiPF03403. PAF-AH_p_II. 1 hit.
[Graphical view]
PIRSFiPIRSF018169. PAF_acetylhydrolase. 1 hit.
SUPFAMiSSF53474. SSF53474. 3 hits.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Plasma platelet-activating factor acetylhydrolase is a secreted phospholipase A2 with a catalytic triad."
    Tjoelker L.W., Eberhardt C., Unger J., le Trong H., Zimmerman G.A., McIntyre T.M., Stafforini D.M., Prescott S.M., Gray P.W.
    J. Biol. Chem. 270:25481-25487(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Spleen.

Entry informationi

Entry nameiPAFA_CANFA
AccessioniPrimary (citable) accession number: Q28262
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1997
Last sequence update: October 31, 1997
Last modified: January 6, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.