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Protein

Tubulin-specific chaperone D

Gene

TBCD

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Tubulin-folding protein implicated in the first step of the tubulin folding pathway and required for tubulin complex assembly. Involved in the regulation of microtubule polymerization or depolymerization, it modulates microtubule dynamics by capturing GTP-bound beta-tubulin (TUBB). Its ability to interact with beta tubulin is regulated via its interaction with ARL2. Acts as a GTPase-activating protein (GAP) for ARL2. Induces microtubule disruption in absence of ARL2. Increases degradation of beta tubulin, when overexpressed in polarized cells. Promotes epithelial cell detachment, a process antagonized by ARL2. Induces tight adherens and tight junctions disassembly at the lateral cell membrane. Required for correct assembly and maintenance of the mitotic spindle, and proper progression of mitosis. Involved in neuron morphogenesis.By similarity2 Publications

GO - Molecular functioni

  • beta-tubulin binding Source: UniProtKB
  • GTPase activator activity Source: UniProtKB

GO - Biological processi

  • adherens junction assembly Source: UniProtKB
  • bicellular tight junction assembly Source: UniProtKB
  • cell morphogenesis involved in neuron differentiation Source: UniProtKB
  • mitotic cell cycle Source: UniProtKB
  • negative regulation of cell-substrate adhesion Source: UniProtKB
  • negative regulation of microtubule polymerization Source: UniProtKB
  • post-chaperonin tubulin folding pathway Source: Ensembl
  • protein folding Source: UniProtKB
  • tubulin complex assembly Source: GO_Central

Keywordsi

Molecular functionChaperone, GTPase activation

Names & Taxonomyi

Protein namesi
Recommended name:
Tubulin-specific chaperone D
Alternative name(s):
Beta-tubulin cofactor D
Tubulin-folding cofactor D
Gene namesi
Name:TBCD
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 19

Organism-specific databases

VGNCiVGNC:35652 TBCD

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000800481 – 1199Tubulin-specific chaperone DAdd BLAST1199

Proteomic databases

PaxDbiQ28205
PRIDEiQ28205

Expressioni

Gene expression databases

BgeeiENSBTAG00000015414
ExpressionAtlasiQ28205 baseline and differential

Interactioni

Subunit structurei

Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. Interacts with PPP2CB. Part of a supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state. Interacts with ARL2; interaction is enhanced with the GDP-bound form of ARL2. Does not interact with ARL3, ARL4A and ARL4D. Interacts with beta tubulin. Interacts with TBCE (By similarity) (PubMed:12912990, PubMed:17704193).By similarity2 Publications

GO - Molecular functioni

  • beta-tubulin binding Source: UniProtKB

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000020484

Structurei

3D structure databases

ProteinModelPortaliQ28205
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati368 – 406HEAT 1Add BLAST39
Repeati603 – 639HEAT 2Add BLAST37
Repeati757 – 793HEAT 3Add BLAST37
Repeati1111 – 1147HEAT 4Add BLAST37

Sequence similaritiesi

Belongs to the TBCD family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1943 Eukaryota
COG5234 LUCA
GeneTreeiENSGT00390000017103
HOGENOMiHOG000231203
HOVERGENiHBG053297
InParanoidiQ28205
KOiK21767
OMAiEPHEAWH
OrthoDBiEOG091G01IN
TreeFamiTF105754

Family and domain databases

Gene3Di1.25.10.10, 2 hits
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR033162 TBCD
IPR022577 Tubulin_specific_chaperoneD_C
PANTHERiPTHR12658 PTHR12658, 1 hit
PfamiView protein in Pfam
PF12612 TFCD_C, 1 hit
SUPFAMiSSF48371 SSF48371, 4 hits

Sequencei

Sequence statusi: Complete.

Q28205-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSEEPAAG AAEDPVEDPV EDAGEDAALA CGAALESFGE SAETRELLGH
60 70 80 90 100
LPAVLADRSA REGALERFRV IMDKYQEQPH LLDPHLEWML NLLLEFVQNK
110 120 130 140 150
TSPADLVHLA FKFLYIISKV RGYKTFLRLF PHEVADVQPV LDMFTNQNPK
160 170 180 190 200
DHETWETRYM LLLWLSVTCL IPFDFSRLDG NLSQPGQERA STMDRILQVA
210 220 230 240 250
ESYLVVSDKA RDAAAVLVSK FVTRPDVKQK KMASFLDWSL CTLARSSFQT
260 270 280 290 300
IEGVIAMDGT LQALAQIFKH GKREDCLPYA ATVLQCLDSC RLPDSNQTLL
310 320 330 340 350
RKLGVKLVQR LGLTFLKPQV AKWRYQRGCR SLAESLQHSI QNPREPVTQA
360 370 380 390 400
ETPDSDGQDD VPEEVESVIE QLLVGLKDKD TIVRWSAAKG IGRMAGRLPK
410 420 430 440 450
ELADDVTGSV LDCFSFQETD SAWHGGCLAL AELGRRGLLL PSRLSDVVPV
460 470 480 490 500
ILRALTYEEK RGACSVGSNV RDAACYVCWA FARAYEPQEL KPFVAAISSA
510 520 530 540 550
LVIATVFDRD VNCRRAASAA FQENVGRQGT FPHGIDILTT ADYFAVGNRS
560 570 580 590 600
NCFLVISMFI AGFPEYTQPM IEHLVTMKVG HWDGTIRELS AKALRNLAQR
610 620 630 640 650
APEHTAREVF PRLLSMTQSP DLHTRHGAVL ACAEVARSLH TLATQQGRPV
660 670 680 690 700
SDFLDEKAMH GLKQIHQQLY DRQLYRGLGG ELMRQAVCIL IENVALSKMP
710 720 730 740 750
FRGDAVIDGW QWLINDTLKN LHLISSHSRQ HIKEAAVSAL AALCSEYHAQ
760 770 780 790 800
EPGEAEAAAQ EELVKLYLAE LQSPEEMTRC GCALALGALP AFFLKGRLRQ
810 820 830 840 850
VLAGLRAVTH ISPKDVSFAE ARRDALKAIS RICQTVGVRA EGPPDEAVCR
860 870 880 890 900
ENVSQIYCTL LDCLKDYTTD SRGDVGAWVR EAAMTSLMDL TLLLGRNQPE
910 920 930 940 950
LIEAPLCQQL MCCLAQQASE KIDRFRAHAA RVFLALLHAD SPAIPHVPAR
960 970 980 990 1000
PELERLFPRA AVASVNWGAP SQAFPRMARL LGLPAYRYHV LLGLAVSVGG
1010 1020 1030 1040 1050
LTESTVRYST QGLFEYMKEI QNDPAALEDF GGTLLQVFED NLLNDRVSVP
1060 1070 1080 1090 1100
LLKTLDQMLA NGCFDIFTAQ ENHPFCVKLL ALCKEEIKKS KDVQKLRSSI
1110 1120 1130 1140 1150
AVFCGLVQFP GDVRRKVLLQ LFLLLCHPFP VIRKNTASQV YEMVLTYDVV
1160 1170 1180 1190
PTAVLDEVMA VLSSTAWDAE LPVVRAQRNR LCDLLGVPRP QLVPKPAVR
Length:1,199
Mass (Da):133,014
Last modified:November 1, 1996 - v1
Checksum:i2FCF9CCA972599BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U61233 mRNA Translation: AAB17537.1
PIRiT18522
RefSeqiNP_776619.1, NM_174194.2
UniGeneiBt.3974

Genome annotation databases

EnsembliENSBTAT00000020484; ENSBTAP00000020484; ENSBTAG00000015414
GeneIDi281515
KEGGibta:281515

Similar proteinsi

Entry informationi

Entry nameiTBCD_BOVIN
AccessioniPrimary (citable) accession number: Q28205
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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