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Protein

Tubulin-specific chaperone D

Gene

TBCD

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Tubulin-folding protein; involved in the first step of the tubulin folding pathway. Modulates microtubule dynamics by capturing GTP-bound beta-tubulin (TUBB). Acts as a GTPase-activating protein (GAP) for ARL2. Its ability to interact with beta tubulin is regulated via its interaction with ARL2. Induces microtubule disruption in absence of ARL2. Increases degradation of beta tubulin, when overexpressed in polarized cells. Promotes epithelial cell detachment, a process antagonized by ARL2. Induces tight adherens and tight junctions disassembly at the lateral cell membrane.2 Publications

GO - Molecular functioni

  • beta-tubulin binding Source: UniProtKB
  • GTPase activator activity Source: UniProtKB

GO - Biological processi

  • adherens junction assembly Source: UniProtKB
  • bicellular tight junction assembly Source: UniProtKB
  • negative regulation of cell-substrate adhesion Source: UniProtKB
  • negative regulation of microtubule polymerization Source: UniProtKB
  • post-chaperonin tubulin folding pathway Source: Reactome
  • protein folding Source: UniProtKB
  • tubulin complex assembly Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Chaperone, GTPase activation

Enzyme and pathway databases

ReactomeiR-BTA-389977. Post-chaperonin tubulin folding pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Tubulin-specific chaperone D
Alternative name(s):
Beta-tubulin cofactor D
Tubulin-folding cofactor D
Gene namesi
Name:TBCD
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 19

Subcellular locationi

GO - Cellular componenti

  • adherens junction Source: UniProtKB
  • bicellular tight junction Source: UniProtKB
  • cytosol Source: Reactome
  • lateral plasma membrane Source: UniProtKB
  • microtubule Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11991199Tubulin-specific chaperone DPRO_0000080048Add
BLAST

Proteomic databases

PaxDbiQ28205.
PRIDEiQ28205.

Expressioni

Gene expression databases

BgeeiENSBTAG00000015414.
ExpressionAtlasiQ28205. baseline and differential.

Interactioni

Subunit structurei

Interacts with ARL2; interaction is enhanced with the GDP-bound form of ARL2. Does not interact with ARL3, ARL4A and ARL4D. Interacts with beta tubulin (By similarity). Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state. Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. Interacts with PPP2CB. Interacts with ARL2.By similarity2 Publications

GO - Molecular functioni

  • beta-tubulin binding Source: UniProtKB

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000020484.

Structurei

3D structure databases

ProteinModelPortaliQ28205.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati368 – 40639HEAT 1Add
BLAST
Repeati603 – 63937HEAT 2Add
BLAST
Repeati757 – 79337HEAT 3Add
BLAST
Repeati1111 – 114737HEAT 4Add
BLAST

Sequence similaritiesi

Belongs to the TBCD family.Curated
Contains 4 HEAT repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1943. Eukaryota.
COG5234. LUCA.
GeneTreeiENSGT00390000017103.
HOGENOMiHOG000231203.
HOVERGENiHBG053297.
InParanoidiQ28205.
OMAiEYYALET.
OrthoDBiEOG091G01IN.
TreeFamiTF105754.

Family and domain databases

Gene3Di1.25.10.10. 5 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR033162. TBCD.
IPR022577. Tubulin_specific_chaperoneD_C.
[Graphical view]
PANTHERiPTHR12658:SF0. PTHR12658:SF0. 1 hit.
PfamiPF12612. TFCD_C. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.

Sequencei

Sequence statusi: Complete.

Q28205-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSEEPAAG AAEDPVEDPV EDAGEDAALA CGAALESFGE SAETRELLGH
60 70 80 90 100
LPAVLADRSA REGALERFRV IMDKYQEQPH LLDPHLEWML NLLLEFVQNK
110 120 130 140 150
TSPADLVHLA FKFLYIISKV RGYKTFLRLF PHEVADVQPV LDMFTNQNPK
160 170 180 190 200
DHETWETRYM LLLWLSVTCL IPFDFSRLDG NLSQPGQERA STMDRILQVA
210 220 230 240 250
ESYLVVSDKA RDAAAVLVSK FVTRPDVKQK KMASFLDWSL CTLARSSFQT
260 270 280 290 300
IEGVIAMDGT LQALAQIFKH GKREDCLPYA ATVLQCLDSC RLPDSNQTLL
310 320 330 340 350
RKLGVKLVQR LGLTFLKPQV AKWRYQRGCR SLAESLQHSI QNPREPVTQA
360 370 380 390 400
ETPDSDGQDD VPEEVESVIE QLLVGLKDKD TIVRWSAAKG IGRMAGRLPK
410 420 430 440 450
ELADDVTGSV LDCFSFQETD SAWHGGCLAL AELGRRGLLL PSRLSDVVPV
460 470 480 490 500
ILRALTYEEK RGACSVGSNV RDAACYVCWA FARAYEPQEL KPFVAAISSA
510 520 530 540 550
LVIATVFDRD VNCRRAASAA FQENVGRQGT FPHGIDILTT ADYFAVGNRS
560 570 580 590 600
NCFLVISMFI AGFPEYTQPM IEHLVTMKVG HWDGTIRELS AKALRNLAQR
610 620 630 640 650
APEHTAREVF PRLLSMTQSP DLHTRHGAVL ACAEVARSLH TLATQQGRPV
660 670 680 690 700
SDFLDEKAMH GLKQIHQQLY DRQLYRGLGG ELMRQAVCIL IENVALSKMP
710 720 730 740 750
FRGDAVIDGW QWLINDTLKN LHLISSHSRQ HIKEAAVSAL AALCSEYHAQ
760 770 780 790 800
EPGEAEAAAQ EELVKLYLAE LQSPEEMTRC GCALALGALP AFFLKGRLRQ
810 820 830 840 850
VLAGLRAVTH ISPKDVSFAE ARRDALKAIS RICQTVGVRA EGPPDEAVCR
860 870 880 890 900
ENVSQIYCTL LDCLKDYTTD SRGDVGAWVR EAAMTSLMDL TLLLGRNQPE
910 920 930 940 950
LIEAPLCQQL MCCLAQQASE KIDRFRAHAA RVFLALLHAD SPAIPHVPAR
960 970 980 990 1000
PELERLFPRA AVASVNWGAP SQAFPRMARL LGLPAYRYHV LLGLAVSVGG
1010 1020 1030 1040 1050
LTESTVRYST QGLFEYMKEI QNDPAALEDF GGTLLQVFED NLLNDRVSVP
1060 1070 1080 1090 1100
LLKTLDQMLA NGCFDIFTAQ ENHPFCVKLL ALCKEEIKKS KDVQKLRSSI
1110 1120 1130 1140 1150
AVFCGLVQFP GDVRRKVLLQ LFLLLCHPFP VIRKNTASQV YEMVLTYDVV
1160 1170 1180 1190
PTAVLDEVMA VLSSTAWDAE LPVVRAQRNR LCDLLGVPRP QLVPKPAVR
Length:1,199
Mass (Da):133,014
Last modified:November 1, 1996 - v1
Checksum:i2FCF9CCA972599BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U61233 mRNA. Translation: AAB17537.1.
PIRiT18522.
RefSeqiNP_776619.1. NM_174194.2.
UniGeneiBt.3974.

Genome annotation databases

EnsembliENSBTAT00000020484; ENSBTAP00000020484; ENSBTAG00000015414.
GeneIDi281515.
KEGGibta:281515.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U61233 mRNA. Translation: AAB17537.1.
PIRiT18522.
RefSeqiNP_776619.1. NM_174194.2.
UniGeneiBt.3974.

3D structure databases

ProteinModelPortaliQ28205.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000020484.

Proteomic databases

PaxDbiQ28205.
PRIDEiQ28205.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000020484; ENSBTAP00000020484; ENSBTAG00000015414.
GeneIDi281515.
KEGGibta:281515.

Organism-specific databases

CTDi6904.

Phylogenomic databases

eggNOGiKOG1943. Eukaryota.
COG5234. LUCA.
GeneTreeiENSGT00390000017103.
HOGENOMiHOG000231203.
HOVERGENiHBG053297.
InParanoidiQ28205.
OMAiEYYALET.
OrthoDBiEOG091G01IN.
TreeFamiTF105754.

Enzyme and pathway databases

ReactomeiR-BTA-389977. Post-chaperonin tubulin folding pathway.

Gene expression databases

BgeeiENSBTAG00000015414.
ExpressionAtlasiQ28205. baseline and differential.

Family and domain databases

Gene3Di1.25.10.10. 5 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR033162. TBCD.
IPR022577. Tubulin_specific_chaperoneD_C.
[Graphical view]
PANTHERiPTHR12658:SF0. PTHR12658:SF0. 1 hit.
PfamiPF12612. TFCD_C. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiTBCD_BOVIN
AccessioniPrimary (citable) accession number: Q28205
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.