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Protein

Cyclic nucleotide-gated cation channel beta-1

Gene

CNGB1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of cyclic nucleotide-gated (CNG) channels, nonselective cation channels, which play important roles in both visual and olfactory signal transduction. When associated with CNGA1, it is involved in the regulation of ion flow into the rod photoreceptor outer segment (ROS), in response to light-induced alteration of the levels of intracellular cGMP (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei1144 – 11441cAMPSequence analysis
Binding sitei1156 – 11561cAMPSequence analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1084 – 1222139cAMPBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel

Keywords - Biological processi

Ion transport, Olfaction, Sensory transduction, Transport, Vision

Keywords - Ligandi

cAMP, cAMP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic nucleotide-gated cation channel beta-1
Alternative name(s):
240 kDa protein of rod photoreceptor CNG-channel
Cyclic nucleotide-gated cation channel 4
Short name:
CNG channel 4
Short name:
CNG-4
Short name:
CNG4
Cyclic nucleotide-gated cation channel gamma
Cyclic nucleotide-gated cation channel modulatory subunit
Cyclic nucleotide-gated channel beta-1
Short name:
CNG channel beta-1
Glutamic acid-rich protein
Short name:
GARP
Gene namesi
Name:CNGB1
Synonyms:CNCG4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 770770CytoplasmicSequence analysisAdd
BLAST
Transmembranei771 – 78919Helical; Name=H1Sequence analysisAdd
BLAST
Topological domaini790 – 80314ExtracellularSequence analysisAdd
BLAST
Transmembranei804 – 82219Helical; Name=H2Sequence analysisAdd
BLAST
Topological domaini823 – 84725CytoplasmicSequence analysisAdd
BLAST
Transmembranei848 – 86720Helical; Name=H3Sequence analysisAdd
BLAST
Topological domaini868 – 90437ExtracellularSequence analysisAdd
BLAST
Transmembranei905 – 92723Helical; Name=H4Sequence analysisAdd
BLAST
Topological domaini928 – 97144CytoplasmicSequence analysisAdd
BLAST
Transmembranei972 – 99120Helical; Name=H5Sequence analysisAdd
BLAST
Topological domaini992 – 107584ExtracellularSequence analysisAdd
BLAST
Transmembranei1076 – 109621Helical; Name=H6Sequence analysisAdd
BLAST
Topological domaini1097 – 1394298CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13941394Cyclic nucleotide-gated cation channel beta-1PRO_0000005555Add
BLAST

Proteomic databases

PaxDbiQ28181.
PRIDEiQ28181.

Expressioni

Tissue specificityi

Retina, testis, kidney, heart and brain.

Interactioni

Subunit structurei

Tetramer formed of three CNGA1 and one CNGB1 modulatory subunits.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself12EBI-6979031,EBI-6979031
O026983EBI-6979031,EBI-7079806
GNAT1P046954EBI-6979031,EBI-7052221
GUCY2DP552035EBI-6979031,EBI-6943076
NRMQ32LM84EBI-6979031,EBI-7079707

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

DIPiDIP-47448N.
IntActiQ28181. 8 interactions.
STRINGi9913.ENSBTAP00000032365.

Structurei

3D structure databases

DisProtiDP00441.
ProteinModelPortaliQ28181.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi682 – 69211IQ-likeAdd
BLAST

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0499. Eukaryota.
ENOG410ZJ5U. LUCA.
HOGENOMiHOG000231425.
HOVERGENiHBG051038.
InParanoidiQ28181.
KOiK04952.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR032944. CNGB1.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PANTHERiPTHR10217:SF386. PTHR10217:SF386. 1 hit.
PfamiPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Isoform CNG4D is the most frequent isoform (CNG4D:CNG4C:CNG4E = 20:2:1) in testis.

Isoform CNG4A (identifier: Q28181-6) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLGWVQRVLP QPPGTPQKTK QEEEGTEPEP ELEPKPETAP EETELEEVSL
60 70 80 90 100
PPEEPCVGKE VAAVTLGPQG TQETALTPPT SLQAQVSVAP EAHSSPRGWV
110 120 130 140 150
LTWLRKGVEK VVPQPAHSSR PSQNIAAGLE SPDQQAGAQI LGQCGTGGSD
160 170 180 190 200
EPSEPSRAED PGPGPWLLRW FEQNLEKMLP QPPKISEGWR DEPTDAALGP
210 220 230 240 250
EPPGPALEIK PMLQAQESPS LPAPGPPEPE EEPIPEPQPT IQASSLPPPQ
260 270 280 290 300
DSARLMAWIL HRLEMALPQP VIRGKGGEQE SDAPVTCDVQ TISILPGEQE
310 320 330 340 350
ESHLILEEVD PHWEEDEHQE GSTSTSPRTS EAAPADEEKG KVVEQTPREL
360 370 380 390 400
PRIQEEKEDE EEEKEDGEEE EEEGREKEEE EGEEKEEEEG REKEEEEGEK
410 420 430 440 450
KEEEGREKEE EEGGEKEDEE GREKEEEEGR GKEEEEGGEK EEEEGRGKEE
460 470 480 490 500
VEGREEEEDE EEEQDHSVLL DSYLVPQSEE DRSEESETQD QSEVGGAQAQ
510 520 530 540 550
GEVGGAQALS EESETQDQSE VGGAQDQSEV GGAQAQGEVG GAQEQDGVGG
560 570 580 590 600
AQDQSTSHQE LQEEALADSS GVPATEEHPE LQVEDADADS RPLIAEENPP
610 620 630 640 650
SPVQLPLSPA KSDTLAVPGS ATGSLRKRLP SQDDEAEELK MLSPAASPVV
660 670 680 690 700
AWSDPTSPQG TDDQDRATST ASQNSAIIND RLQELVKLFK ERTEKVKEKL
710 720 730 740 750
IDPDVTSDEE SPKPSPAKKA PEPAPEVKPA EAGQVEEEHY CEMLCCKFKR
760 770 780 790 800
RPWKKYQFPQ SIDPLTNLMY ILWLFFVVLA WNWNCWLIPV RWAFPYQTPD
810 820 830 840 850
NIHLWLLMDY LCDLIYLLDI TVFQMRLQFV RGGDIITDKK EMRNNYVKSQ
860 870 880 890 900
RFKMDMLCLL PLDLLYLKFG VNPLLRLPRC LKYMAFFEFN NRLESILSKA
910 920 930 940 950
YVYRVIRTTA YLLYSLHLNS CLYYWASAYE GLGSTHWVYD GVGNSYIRCY
960 970 980 990 1000
YWAVKTLITI GGLPDPRTLF EIVFQGLNYF TGVFAFSVMI GQMRDVVGAA
1010 1020 1030 1040 1050
TAGQTYYRSC MDSTVKYMNF YKIPRSVQNR VKTWYEYTWH SQGMLDESEL
1060 1070 1080 1090 1100
MVQLPDKMRL DLAIDVNYSI VSKVALFQGC DRQMIFDMLK RLRSVVYLPN
1110 1120 1130 1140 1150
DYVCKKGEIG REMYIIQAGQ VQVLGGPDGK SVLVTLKAGS VFGEISLLAV
1160 1170 1180 1190 1200
GGGNRRTANV VAHGFTNLFI LDKKDLNEIL VHYPESQKLL RKKARRMLRN
1210 1220 1230 1240 1250
NNKPKEKSVL ILPPRAGTPK LFNAALAAAG KMGAKGGRGG RLALLRARLK
1260 1270 1280 1290 1300
ELAALEAAAR QQQLLEQAKS SEDAAVGEEG SASPEQPPRP EPPAPEAPAP
1310 1320 1330 1340 1350
EPTAPEPLAP EAPAPEAPAP SSPPPASQER PEGDKDAARP EEHPVRIHVT
1360 1370 1380 1390
LGPDPSEQIL LVEVPEKQEE KEKKEEETEE KEEGEEARKE KEEE
Length:1,394
Mass (Da):155,065
Last modified:November 1, 1996 - v1
Checksum:iEE6DA559BE3744A7
GO
Isoform CNG4C (identifier: Q28181-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-456: Missing.
     457-465: EEDEEEEQD → MRAGQKGRC
     482-482: R → Q

Show »
Length:938
Mass (Da):104,490
Checksum:iD19659E834FC0CFB
GO
Isoform CNG4D (identifier: Q28181-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-456: Missing.
     457-465: EEDEEEEQD → MRAGQKGRC
     522-530: Missing.

Show »
Length:929
Mass (Da):103,647
Checksum:i328242743FEF5981
GO
Isoform CNG4E (identifier: Q28181-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-456: Missing.
     457-465: EEDEEEEQD → MRAGQKGRC
     515-532: Missing.

Show »
Length:920
Mass (Da):102,745
Checksum:i86C852E74F1F0BF7
GO
Isoform GARP1 (identifier: Q28181-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     341-341: K → E
     482-482: R → Q
     499-499: A → T
     572-590: VPATEEHPELQVEDADADS → GSFQMSPFEALQECEALKR
     591-1394: Missing.

Note: In the rod cells, the Cngb1 locus encodes the cyclic nucleotide-gated cation channel beta-1 subunit and several glutamic-acid-rich proteins (GARPs).
Show »
Length:590
Mass (Da):64,511
Checksum:i5F56252F6CA3686B
GO
Isoform GARP2 (identifier: Q28181-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     292-299: ISILPGEQ → RVVAAGSL
     300-1394: Missing.

Note: In the rod cells, the Cngb1 locus encodes the cyclic nucleotide-gated cation channel beta-1 subunit and several glutamic-acid-rich proteins (GARPs).
Show »
Length:299
Mass (Da):31,967
Checksum:i450D2DA6D68006C5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1283 – 12831S → A in CAA64367 (PubMed:8626431).Curated
Sequence conflicti1289 – 12891R → A in CAA64367 (PubMed:8626431).Curated
Sequence conflicti1336 – 13361D → E in CAA64367 (PubMed:8626431).Curated
Sequence conflicti1338 – 13381A → AA in CAA64367 (PubMed:8626431).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 456456Missing in isoform CNG4C, isoform CNG4E and isoform CNG4D. 1 PublicationVSP_037919Add
BLAST
Alternative sequencei292 – 2998ISILPGEQ → RVVAAGSL in isoform GARP2. CuratedVSP_037910
Alternative sequencei300 – 13941095Missing in isoform GARP2. CuratedVSP_037911Add
BLAST
Alternative sequencei341 – 3411K → E in isoform GARP1. 1 PublicationVSP_037912
Alternative sequencei457 – 4659EEDEEEEQD → MRAGQKGRC in isoform CNG4C, isoform CNG4E and isoform CNG4D. 1 PublicationVSP_037920
Alternative sequencei482 – 4821R → Q in isoform GARP1 and isoform CNG4C. 2 PublicationsVSP_037913
Alternative sequencei499 – 4991A → T in isoform GARP1. 1 PublicationVSP_037914
Alternative sequencei515 – 53218Missing in isoform CNG4E. CuratedVSP_037915Add
BLAST
Alternative sequencei522 – 5309Missing in isoform CNG4D. CuratedVSP_037916
Alternative sequencei572 – 59019VPATE…ADADS → GSFQMSPFEALQECEALKR in isoform GARP1. 1 PublicationVSP_037917Add
BLAST
Alternative sequencei591 – 1394804Missing in isoform GARP1. 1 PublicationVSP_037918Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89626 mRNA. Translation: CAA61769.1.
X94707 mRNA. Translation: CAA64367.1.
M61185 mRNA. Translation: AAA30536.1.
PIRiA40437.
RefSeqiNP_001129112.1. NM_001135640.1. [Q28181-4]
NP_001129113.1. NM_001135641.1. [Q28181-5]
NP_851362.1. NM_181019.2. [Q28181-6]
UniGeneiBt.5064.

Genome annotation databases

GeneIDi281702.
KEGGibta:281702.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89626 mRNA. Translation: CAA61769.1.
X94707 mRNA. Translation: CAA64367.1.
M61185 mRNA. Translation: AAA30536.1.
PIRiA40437.
RefSeqiNP_001129112.1. NM_001135640.1. [Q28181-4]
NP_001129113.1. NM_001135641.1. [Q28181-5]
NP_851362.1. NM_181019.2. [Q28181-6]
UniGeneiBt.5064.

3D structure databases

DisProtiDP00441.
ProteinModelPortaliQ28181.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-47448N.
IntActiQ28181. 8 interactions.
STRINGi9913.ENSBTAP00000032365.

Proteomic databases

PaxDbiQ28181.
PRIDEiQ28181.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281702.
KEGGibta:281702.

Organism-specific databases

CTDi1258.

Phylogenomic databases

eggNOGiKOG0499. Eukaryota.
ENOG410ZJ5U. LUCA.
HOGENOMiHOG000231425.
HOVERGENiHBG051038.
InParanoidiQ28181.
KOiK04952.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR032944. CNGB1.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PANTHERiPTHR10217:SF386. PTHR10217:SF386. 1 hit.
PfamiPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A 240 kDa protein represents the complete beta subunit of the cyclic nucleotide-gated channel from rod photoreceptor."
    Koerschen H.G., Illing M., Seifert R., Sesti F., Williams A., Gotzes S., Colville C., Mueller F., Dose A., Godde M., Molday L., Kaupp U.B., Molday R.S.
    Neuron 15:627-636(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM CNG4A), PARTIAL PROTEIN SEQUENCE.
  2. "Molecular cloning and expression of the modulatory subunit of the cyclic nucleotide-gated cation channel."
    Biel M., Zong X., Ludwig A., Sautter A., Hofmann F.
    J. Biol. Chem. 271:6349-6355(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM CNG4C), ALTERNATIVE SPLICING (ISOFORMS CNG4D AND CNG4E).
    Tissue: Testis.
  3. "The amino acid sequence of a glutamic acid-rich protein from bovine retina as deduced from the cDNA sequence."
    Sugimoto Y., Yatsunami K., Tsujimoto M., Khorana H.G., Ichikawa A.
    Proc. Natl. Acad. Sci. U.S.A. 88:3116-3119(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM GARP1).
    Tissue: Retina.
  4. Cote R.H.
    Unpublished observations (MAY-2009)
    Cited for: ALTERNATIVE SPLICING (ISOFORMS GARP1 AND GARP2).
  5. "Molecular mechanism for 3:1 subunit stoichiometry of rod cyclic nucleotide-gated ion channels."
    Shuart N.G., Haitin Y., Camp S.S., Black K.D., Zagotta W.N.
    Nat. Commun. 2:457-457(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.

Entry informationi

Entry nameiCNGB1_BOVIN
AccessioniPrimary (citable) accession number: Q28181
Secondary accession number(s): Q03861, Q28082
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.