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Q28178

- TSP1_BOVIN

UniProt

Q28178 - TSP1_BOVIN

Protein

Thrombospondin-1

Gene

THBS1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties By similarity. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors By similarity.By similarity

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. extracellular matrix binding Source: InterPro
    3. heparin binding Source: UniProtKB-KW

    GO - Biological processi

    1. behavioral response to pain Source: UniProtKB
    2. cell adhesion Source: UniProtKB-KW
    3. inflammatory response Source: InterPro
    4. negative regulation of angiogenesis Source: InterPro
    5. response to endoplasmic reticulum stress Source: UniProtKB
    6. response to unfolded protein Source: UniProtKB-KW

    Keywords - Biological processi

    Cell adhesion, Unfolded protein response

    Keywords - Ligandi

    Calcium, Heparin-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thrombospondin-1
    Gene namesi
    Name:THBS1
    Synonyms:TSP-1, TSP1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    Endoplasmic reticulum By similarity. Sarcoplasmic reticulum By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB
    2. extracellular space Source: InterPro
    3. sarcoplasmic reticulum Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Sarcoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818By similarityAdd
    BLAST
    Chaini19 – 11701152Thrombospondin-1PRO_0000035841Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi171 ↔ 232By similarity
    Glycosylationi248 – 2481N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi270 – 270InterchainCurated
    Disulfide bondi274 – 274InterchainCurated
    Glycosylationi360 – 3601N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi391 ↔ 423By similarity
    Disulfide bondi395 ↔ 428By similarity
    Disulfide bondi406 ↔ 413By similarity
    Disulfide bondi447 ↔ 484By similarity
    Disulfide bondi451 ↔ 489By similarity
    Disulfide bondi462 ↔ 474By similarity
    Disulfide bondi504 ↔ 541By similarity
    Disulfide bondi508 ↔ 546By similarity
    Disulfide bondi519 ↔ 531By similarity
    Disulfide bondi551 ↔ 562By similarity
    Disulfide bondi556 ↔ 572By similarity
    Disulfide bondi575 ↔ 586By similarity
    Disulfide bondi592 ↔ 608By similarity
    Disulfide bondi599 ↔ 617By similarity
    Disulfide bondi620 ↔ 644By similarity
    Disulfide bondi650 ↔ 663By similarity
    Disulfide bondi657 ↔ 676By similarity
    Disulfide bondi678 ↔ 689By similarity
    Disulfide bondi705 ↔ 713By similarity
    Glycosylationi708 – 7081N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi718 ↔ 738By similarity
    Disulfide bondi754 ↔ 774By similarity
    Disulfide bondi777 ↔ 797By similarity
    Disulfide bondi813 ↔ 833By similarity
    Disulfide bondi836 ↔ 856By similarity
    Disulfide bondi874 ↔ 894By similarity
    Disulfide bondi910 ↔ 930By similarity
    Disulfide bondi946 ↔ 1167By similarity
    Glycosylationi1067 – 10671N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1085 – 10851N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ28178.
    PRIDEiQ28178.

    PTM databases

    UniCarbKBiQ28178.

    Expressioni

    Tissue specificityi

    Odontoblasts.1 Publication

    Interactioni

    Subunit structurei

    Homotrimer; disulfide-linked By similarity. Can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1, alpha-V/beta-3 and alpha-IIb/beta-3. Binds heparin. Interacts (via the TSP type I repeats) with CD36; the interaction conveys an antiangiogenic effect. Interacts (via the TSP type I repeats) with HRG; the interaction blocks the antiangiogenic effect of THBS1 with CD36 By similarity. Interacts with ATF6 (via lumenal domain) By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ28178. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ28178.
    SMRiQ28178. Positions 25-233, 434-546, 549-1169.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini65 – 270206Laminin G-likeAdd
    BLAST
    Domaini316 – 37358VWFCPROSITE-ProRule annotationAdd
    BLAST
    Domaini379 – 42951TSP type-1 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini435 – 49056TSP type-1 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini492 – 54756TSP type-1 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini547 – 58741EGF-like 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini646 – 69045EGF-like 2PROSITE-ProRule annotationAdd
    BLAST
    Repeati691 – 72636TSP type-3 1Add
    BLAST
    Repeati727 – 76236TSP type-3 2Add
    BLAST
    Repeati763 – 78523TSP type-3 3Add
    BLAST
    Repeati786 – 82136TSP type-3 4Add
    BLAST
    Repeati822 – 84423TSP type-3 5Add
    BLAST
    Repeati845 – 88238TSP type-3 6Add
    BLAST
    Repeati883 – 91836TSP type-3 7Add
    BLAST
    Repeati919 – 95436TSP type-3 8Add
    BLAST
    Domaini958 – 1170213TSP C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni47 – 9549Heparin-bindingBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi926 – 9283Cell attachment siteSequence Analysis

    Sequence similaritiesi

    Belongs to the thrombospondin family.Curated
    Contains 2 EGF-like domains.PROSITE-ProRule annotation
    Contains 1 laminin G-like domain.Curated
    Contains 1 TSP C-terminal (TSPC) domain.PROSITE-ProRule annotation
    Contains 3 TSP type-1 domains.PROSITE-ProRule annotation
    Contains 8 TSP type-3 repeats.PROSITE-ProRule annotation
    Contains 1 VWFC domain.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG12793.
    HOGENOMiHOG000007542.
    HOVERGENiHBG018006.
    InParanoidiQ28178.
    KOiK16857.

    Family and domain databases

    Gene3Di2.60.120.200. 2 hits.
    4.10.1080.10. 2 hits.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000742. EG-like_dom.
    IPR001881. EGF-like_Ca-bd_dom.
    IPR013032. EGF-like_CS.
    IPR001791. Laminin_G.
    IPR028499. Thrombospondin-1.
    IPR000884. Thrombospondin_1_rpt.
    IPR003367. Thrombospondin_3-like_rpt.
    IPR017897. Thrombospondin_3_rpt.
    IPR008859. Thrombospondin_C.
    IPR028974. TSP_type-3_rpt.
    IPR001007. VWF_C.
    [Graphical view]
    PANTHERiPTHR10199:SF78. PTHR10199:SF78. 1 hit.
    PfamiPF07645. EGF_CA. 1 hit.
    PF00090. TSP_1. 3 hits.
    PF02412. TSP_3. 7 hits.
    PF05735. TSP_C. 1 hit.
    PF00093. VWC. 1 hit.
    [Graphical view]
    SMARTiSM00181. EGF. 3 hits.
    SM00209. TSP1. 3 hits.
    SM00210. TSPN. 1 hit.
    SM00214. VWC. 1 hit.
    [Graphical view]
    SUPFAMiSSF103647. SSF103647. 3 hits.
    SSF49899. SSF49899. 2 hits.
    SSF82895. SSF82895. 3 hits.
    PROSITEiPS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 2 hits.
    PS50092. TSP1. 3 hits.
    PS51234. TSP3. 8 hits.
    PS51236. TSP_CTER. 1 hit.
    PS01208. VWFC_1. 1 hit.
    PS50184. VWFC_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q28178-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLAWGLGVL LLLHACGSNR IPESGGDNSV FDIFELTGAA RKRSGRRLVK     50
    GPDPSSPAFR IEDANLIPPV PDKKFQDLVD AVRAEKGFLL LASLRQMKKT 100
    RGTLLAVERK DHSGQVFSVI SNGKAGTLDL SLTVQGKQHV VSVEEALLAT 150
    GQWKSITLFV QEDRAQLYID CEKMENAELD VPIQSIFTRD LASIARLRIA 200
    KGGVNDNFQG VLQNVRFVFG TTPEDILRNK GCSSSTSVFV TLDNNVVNGS 250
    SPAIRTDYIG HKTKDLQAIC GISCDELSSM VLELRGLRTI VTTLQDSIRK 300
    VTEENKELAN ELRRPPLCYH NGVQYRTGDE WTVDSCTECR CQNSVTICKK 350
    VSCPIMPCSN ATVPDGECCP RCWPSDSADD GWSPWSEWTS CSVTCGNGIQ 400
    QRGRSCDSLN NRCEGSSVQT RTCHIQECDK RFKQDGGWSH WSPWSSCSVT 450
    CGDGVITRIR LCNSPSPQMN GKPCEGKARE TKACQKDSCP INGGWGPWSP 500
    WDICSVTCGG GVQKRSRLCN NPKPQFGGKD CVGDVTENQI CNKQDCPIDG 550
    CLSNPCFAGV QCTSYPDGSW KCGACPPGYS GDGVECKDVD ECKEVPDACF 600
    NHNGEHRCEN TDPGYNCLPC PPRFTGSQPF GRGVEHATAN KQVCKPRNPC 650
    TDGTHDCNKN AKCNYLGHYS DPMYRCECKP GYAGNGIICG EDTDLDGWPN 700
    EDLLCVANAT YHCRKDNCPN LPNSGQEDYD KDGIGDACDD DDDNDKIPDD 750
    RDNCPFHYNP AQYDYDRDDV GDRCDNCPYN HNPDQADTDN NGEGDACAAD 800
    IDGDSILNER DNCQYVYNVD QKDTDMDGVG DQCDNCPLEH NPDQLDSDSD 850
    RIGDTCDNNQ DIDEDGHQNN LDNCPYVPNA NQADHDKDGK GDACDHDDDN 900
    DGIPDDRDNC RLVPNPDQKD SDGDGRGDAC KDDFDQDKVP DIDDICPENV 950
    DISETDFRRF QMIPLDPKGT SQNDPNWVVR HQGKELVQTV NCDPGLAVGY 1000
    DEFNAVDFSG TFFINTERDD DYAGFVFGYQ SSSRFYVVMW KQVTQSYWDT 1050
    NPTRAQGYSG LSVKVVNSTT GPGEHLRNAL WHTGNTSGQV RTLWHDPRHI 1100
    GWKDFTAYRW HLSHRPKTGF IRVVMYEGKK IMADSGPIYD KTYAGGRLGL 1150
    FVFSQEMVFF SDLKYECRDS 1170
    Length:1,170
    Mass (Da):129,534
    Last modified:December 1, 2000 - v2
    Checksum:i0DD6ADF3E5FA031A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti805 – 8051S → G in CAA60950. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB005287 mRNA. Translation: BAA21115.1.
    X87618 mRNA. Translation: CAA60950.1.
    X87619 mRNA. Translation: CAA60951.1.
    PIRiS55501.
    RefSeqiNP_776621.1. NM_174196.1.
    UniGeneiBt.109568.

    Genome annotation databases

    GeneIDi281530.
    KEGGibta:281530.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB005287 mRNA. Translation: BAA21115.1 .
    X87618 mRNA. Translation: CAA60950.1 .
    X87619 mRNA. Translation: CAA60951.1 .
    PIRi S55501.
    RefSeqi NP_776621.1. NM_174196.1.
    UniGenei Bt.109568.

    3D structure databases

    ProteinModelPortali Q28178.
    SMRi Q28178. Positions 25-233, 434-546, 549-1169.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q28178. 1 interaction.

    PTM databases

    UniCarbKBi Q28178.

    Proteomic databases

    PaxDbi Q28178.
    PRIDEi Q28178.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 281530.
    KEGGi bta:281530.

    Organism-specific databases

    CTDi 7057.

    Phylogenomic databases

    eggNOGi NOG12793.
    HOGENOMi HOG000007542.
    HOVERGENi HBG018006.
    InParanoidi Q28178.
    KOi K16857.

    Miscellaneous databases

    NextBioi 20805487.

    Family and domain databases

    Gene3Di 2.60.120.200. 2 hits.
    4.10.1080.10. 2 hits.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000742. EG-like_dom.
    IPR001881. EGF-like_Ca-bd_dom.
    IPR013032. EGF-like_CS.
    IPR001791. Laminin_G.
    IPR028499. Thrombospondin-1.
    IPR000884. Thrombospondin_1_rpt.
    IPR003367. Thrombospondin_3-like_rpt.
    IPR017897. Thrombospondin_3_rpt.
    IPR008859. Thrombospondin_C.
    IPR028974. TSP_type-3_rpt.
    IPR001007. VWF_C.
    [Graphical view ]
    PANTHERi PTHR10199:SF78. PTHR10199:SF78. 1 hit.
    Pfami PF07645. EGF_CA. 1 hit.
    PF00090. TSP_1. 3 hits.
    PF02412. TSP_3. 7 hits.
    PF05735. TSP_C. 1 hit.
    PF00093. VWC. 1 hit.
    [Graphical view ]
    SMARTi SM00181. EGF. 3 hits.
    SM00209. TSP1. 3 hits.
    SM00210. TSPN. 1 hit.
    SM00214. VWC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103647. SSF103647. 3 hits.
    SSF49899. SSF49899. 2 hits.
    SSF82895. SSF82895. 3 hits.
    PROSITEi PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 2 hits.
    PS50092. TSP1. 3 hits.
    PS51234. TSP3. 8 hits.
    PS51236. TSP_CTER. 1 hit.
    PS01208. VWFC_1. 1 hit.
    PS50184. VWFC_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning of bovine thrombospondin 1 and its expression in odontoblasts and predentin."
      Ueno A., Yamashita K., Nagata T., Tsurumi C., Miwa Y., Kitamura S., Inoue H.
      Biochim. Biophys. Acta 1382:17-22(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], POSSIBLE FUNCTION, TISSUE SPECIFICITY.
      Strain: Holstein.
      Tissue: Tooth.
    2. "Cloning and sequencing of bovine thrombospondin stimulatory effect of TGF-beta."
      Zafar R.S., Moll Y.D., Womack J.F., Walz D.A.
      Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-18 AND 710-1170.
      Tissue: Aortic endothelium.

    Entry informationi

    Entry nameiTSP1_BOVIN
    AccessioniPrimary (citable) accession number: Q28178
    Secondary accession number(s): Q28179
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3