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Protein

Thrombospondin-1

Gene

THBS1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties (By similarity). May play a role in dentinogenesis and/or maintenance of dentin and dental pulp. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHeparin-binding
Biological processCell adhesion, Unfolded protein response
LigandCalcium

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombospondin-1
Gene namesi
Name:THBS1
Synonyms:TSP-1, TSP1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18By similarityAdd BLAST18
ChainiPRO_000003584119 – 1170Thrombospondin-1Add BLAST1152

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi171 ↔ 232By similarity
Glycosylationi248N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi270InterchainCurated
Disulfide bondi274InterchainCurated
Glycosylationi360N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi391 ↔ 423By similarity
Disulfide bondi395 ↔ 428By similarity
Disulfide bondi406 ↔ 413By similarity
Disulfide bondi447 ↔ 484By similarity
Disulfide bondi451 ↔ 489By similarity
Disulfide bondi462 ↔ 474By similarity
Disulfide bondi504 ↔ 541By similarity
Disulfide bondi508 ↔ 546By similarity
Disulfide bondi519 ↔ 531By similarity
Disulfide bondi551 ↔ 562By similarity
Disulfide bondi556 ↔ 572By similarity
Disulfide bondi575 ↔ 586By similarity
Disulfide bondi592 ↔ 608By similarity
Disulfide bondi599 ↔ 617By similarity
Disulfide bondi620 ↔ 644By similarity
Disulfide bondi650 ↔ 663By similarity
Disulfide bondi657 ↔ 676By similarity
Disulfide bondi678 ↔ 689By similarity
Disulfide bondi705 ↔ 713By similarity
Glycosylationi708N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi718 ↔ 738By similarity
Disulfide bondi754 ↔ 774By similarity
Disulfide bondi777 ↔ 797By similarity
Disulfide bondi813 ↔ 833By similarity
Disulfide bondi836 ↔ 856By similarity
Disulfide bondi874 ↔ 894By similarity
Disulfide bondi910 ↔ 930By similarity
Disulfide bondi946 ↔ 1167By similarity
Glycosylationi1067N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1085N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ28178
PeptideAtlasiQ28178
PRIDEiQ28178

PTM databases

GlyConnecti593
UniCarbKBiQ28178

Expressioni

Tissue specificityi

Odontoblasts.1 Publication

Interactioni

Subunit structurei

Homotrimer; disulfide-linked (By similarity). Can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1, alpha-V/beta-3 and alpha-IIb/beta-3. Binds heparin. Interacts (via the TSP type I repeats) with CD36; the interaction conveys an antiangiogenic effect. Interacts (via the TSP type I repeats) with HRG; the interaction blocks the antiangiogenic effect of THBS1 with CD36 (By similarity). Interacts with ATF6 (via lumenal domain) (By similarity).By similarity

Protein-protein interaction databases

IntActiQ28178, 1 interactor
STRINGi9913.ENSBTAP00000002600

Structurei

3D structure databases

ProteinModelPortaliQ28178
SMRiQ28178
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini65 – 270Laminin G-likeAdd BLAST206
Domaini316 – 373VWFCPROSITE-ProRule annotationAdd BLAST58
Domaini379 – 429TSP type-1 1PROSITE-ProRule annotationAdd BLAST51
Domaini435 – 490TSP type-1 2PROSITE-ProRule annotationAdd BLAST56
Domaini492 – 547TSP type-1 3PROSITE-ProRule annotationAdd BLAST56
Domaini547 – 587EGF-like 1PROSITE-ProRule annotationAdd BLAST41
Domaini646 – 690EGF-like 2PROSITE-ProRule annotationAdd BLAST45
Repeati691 – 726TSP type-3 1Add BLAST36
Repeati727 – 762TSP type-3 2Add BLAST36
Repeati763 – 785TSP type-3 3Add BLAST23
Repeati786 – 821TSP type-3 4Add BLAST36
Repeati822 – 844TSP type-3 5Add BLAST23
Repeati845 – 882TSP type-3 6Add BLAST38
Repeati883 – 918TSP type-3 7Add BLAST36
Repeati919 – 954TSP type-3 8Add BLAST36
Domaini958 – 1170TSP C-terminalPROSITE-ProRule annotationAdd BLAST213

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni47 – 95Heparin-bindingBy similarityAdd BLAST49

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi926 – 928Cell attachment siteSequence analysis3

Sequence similaritiesi

Belongs to the thrombospondin family.Curated

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IFQQ Eukaryota
ENOG410XQKE LUCA
HOGENOMiHOG000007542
HOVERGENiHBG018006
InParanoidiQ28178
KOiK16857

Family and domain databases

Gene3Di2.20.100.10, 3 hits
4.10.1080.10, 2 hits
InterProiView protein in InterPro
IPR026823 cEGF
IPR013320 ConA-like_dom_sf
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR001791 Laminin_G
IPR037349 Thrombospondin
IPR028499 Thrombospondin-1
IPR003367 Thrombospondin_3-like_rpt
IPR017897 Thrombospondin_3_rpt
IPR008859 Thrombospondin_C
IPR000884 TSP1_rpt
IPR036383 TSP1_rpt_sf
IPR028974 TSP_type-3_rpt
IPR001007 VWF_dom
PANTHERiPTHR10199 PTHR10199, 5 hits
PTHR10199:SF78 PTHR10199:SF78, 5 hits
PfamiView protein in Pfam
PF12662 cEGF, 1 hit
PF00090 TSP_1, 3 hits
PF02412 TSP_3, 7 hits
PF05735 TSP_C, 1 hit
PF00093 VWC, 1 hit
SMARTiView protein in SMART
SM00181 EGF, 3 hits
SM00179 EGF_CA, 2 hits
SM00209 TSP1, 3 hits
SM00210 TSPN, 1 hit
SM00214 VWC, 1 hit
SUPFAMiSSF103647 SSF103647, 3 hits
SSF49899 SSF49899, 2 hits
SSF82895 SSF82895, 3 hits
PROSITEiView protein in PROSITE
PS01186 EGF_2, 1 hit
PS50026 EGF_3, 2 hits
PS50092 TSP1, 3 hits
PS51234 TSP3, 8 hits
PS51236 TSP_CTER, 1 hit
PS01208 VWFC_1, 1 hit
PS50184 VWFC_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q28178-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLAWGLGVL LLLHACGSNR IPESGGDNSV FDIFELTGAA RKRSGRRLVK
60 70 80 90 100
GPDPSSPAFR IEDANLIPPV PDKKFQDLVD AVRAEKGFLL LASLRQMKKT
110 120 130 140 150
RGTLLAVERK DHSGQVFSVI SNGKAGTLDL SLTVQGKQHV VSVEEALLAT
160 170 180 190 200
GQWKSITLFV QEDRAQLYID CEKMENAELD VPIQSIFTRD LASIARLRIA
210 220 230 240 250
KGGVNDNFQG VLQNVRFVFG TTPEDILRNK GCSSSTSVFV TLDNNVVNGS
260 270 280 290 300
SPAIRTDYIG HKTKDLQAIC GISCDELSSM VLELRGLRTI VTTLQDSIRK
310 320 330 340 350
VTEENKELAN ELRRPPLCYH NGVQYRTGDE WTVDSCTECR CQNSVTICKK
360 370 380 390 400
VSCPIMPCSN ATVPDGECCP RCWPSDSADD GWSPWSEWTS CSVTCGNGIQ
410 420 430 440 450
QRGRSCDSLN NRCEGSSVQT RTCHIQECDK RFKQDGGWSH WSPWSSCSVT
460 470 480 490 500
CGDGVITRIR LCNSPSPQMN GKPCEGKARE TKACQKDSCP INGGWGPWSP
510 520 530 540 550
WDICSVTCGG GVQKRSRLCN NPKPQFGGKD CVGDVTENQI CNKQDCPIDG
560 570 580 590 600
CLSNPCFAGV QCTSYPDGSW KCGACPPGYS GDGVECKDVD ECKEVPDACF
610 620 630 640 650
NHNGEHRCEN TDPGYNCLPC PPRFTGSQPF GRGVEHATAN KQVCKPRNPC
660 670 680 690 700
TDGTHDCNKN AKCNYLGHYS DPMYRCECKP GYAGNGIICG EDTDLDGWPN
710 720 730 740 750
EDLLCVANAT YHCRKDNCPN LPNSGQEDYD KDGIGDACDD DDDNDKIPDD
760 770 780 790 800
RDNCPFHYNP AQYDYDRDDV GDRCDNCPYN HNPDQADTDN NGEGDACAAD
810 820 830 840 850
IDGDSILNER DNCQYVYNVD QKDTDMDGVG DQCDNCPLEH NPDQLDSDSD
860 870 880 890 900
RIGDTCDNNQ DIDEDGHQNN LDNCPYVPNA NQADHDKDGK GDACDHDDDN
910 920 930 940 950
DGIPDDRDNC RLVPNPDQKD SDGDGRGDAC KDDFDQDKVP DIDDICPENV
960 970 980 990 1000
DISETDFRRF QMIPLDPKGT SQNDPNWVVR HQGKELVQTV NCDPGLAVGY
1010 1020 1030 1040 1050
DEFNAVDFSG TFFINTERDD DYAGFVFGYQ SSSRFYVVMW KQVTQSYWDT
1060 1070 1080 1090 1100
NPTRAQGYSG LSVKVVNSTT GPGEHLRNAL WHTGNTSGQV RTLWHDPRHI
1110 1120 1130 1140 1150
GWKDFTAYRW HLSHRPKTGF IRVVMYEGKK IMADSGPIYD KTYAGGRLGL
1160 1170
FVFSQEMVFF SDLKYECRDS
Length:1,170
Mass (Da):129,534
Last modified:December 1, 2000 - v2
Checksum:i0DD6ADF3E5FA031A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti805S → G in CAA60950 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005287 mRNA Translation: BAA21115.1
X87618 mRNA Translation: CAA60950.1
X87619 mRNA Translation: CAA60951.1
PIRiS55501
RefSeqiNP_776621.1, NM_174196.1
UniGeneiBt.109568

Genome annotation databases

GeneIDi281530
KEGGibta:281530

Similar proteinsi

Entry informationi

Entry nameiTSP1_BOVIN
AccessioniPrimary (citable) accession number: Q28178
Secondary accession number(s): Q28179
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 1, 2000
Last modified: April 25, 2018
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health