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Q28178

- TSP1_BOVIN

UniProt

Q28178 - TSP1_BOVIN

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Protein
Thrombospondin-1
Gene
THBS1, TSP-1, TSP1
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties By similarity. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors By similarity.1 Publication

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. extracellular matrix binding Source: InterPro
  3. heparin binding Source: UniProtKB-KW

GO - Biological processi

  1. behavioral response to pain Source: UniProtKB
  2. cell adhesion Source: UniProtKB-KW
  3. inflammatory response Source: InterPro
  4. negative regulation of angiogenesis Source: InterPro
  5. response to endoplasmic reticulum stress Source: UniProtKB
  6. response to unfolded protein Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell adhesion, Unfolded protein response

Keywords - Ligandi

Calcium, Heparin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombospondin-1
Gene namesi
Name:THBS1
Synonyms:TSP-1, TSP1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Endoplasmic reticulum By similarity. Sarcoplasmic reticulum By similarity

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB
  2. extracellular space Source: InterPro
  3. sarcoplasmic reticulum Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818 By similarity
Add
BLAST
Chaini19 – 11701152Thrombospondin-1
PRO_0000035841Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi171 ↔ 232 By similarity
Glycosylationi248 – 2481N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi270 – 270Interchain Inferred
Disulfide bondi274 – 274Interchain Inferred
Glycosylationi360 – 3601N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi391 ↔ 423 By similarity
Disulfide bondi395 ↔ 428 By similarity
Disulfide bondi406 ↔ 413 By similarity
Disulfide bondi447 ↔ 484 By similarity
Disulfide bondi451 ↔ 489 By similarity
Disulfide bondi462 ↔ 474 By similarity
Disulfide bondi504 ↔ 541 By similarity
Disulfide bondi508 ↔ 546 By similarity
Disulfide bondi519 ↔ 531 By similarity
Disulfide bondi551 ↔ 562 By similarity
Disulfide bondi556 ↔ 572 By similarity
Disulfide bondi575 ↔ 586 By similarity
Disulfide bondi592 ↔ 608 By similarity
Disulfide bondi599 ↔ 617 By similarity
Disulfide bondi620 ↔ 644 By similarity
Disulfide bondi650 ↔ 663 By similarity
Disulfide bondi657 ↔ 676 By similarity
Disulfide bondi678 ↔ 689 By similarity
Disulfide bondi705 ↔ 713 By similarity
Glycosylationi708 – 7081N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi718 ↔ 738 By similarity
Disulfide bondi754 ↔ 774 By similarity
Disulfide bondi777 ↔ 797 By similarity
Disulfide bondi813 ↔ 833 By similarity
Disulfide bondi836 ↔ 856 By similarity
Disulfide bondi874 ↔ 894 By similarity
Disulfide bondi910 ↔ 930 By similarity
Disulfide bondi946 ↔ 1167 By similarity
Glycosylationi1067 – 10671N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1085 – 10851N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ28178.
PRIDEiQ28178.

PTM databases

UniCarbKBiQ28178.

Expressioni

Tissue specificityi

Odontoblasts.1 Publication

Interactioni

Subunit structurei

Homotrimer; disulfide-linked By similarity. Can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1, alpha-V/beta-3 and alpha-IIb/beta-3. Binds heparin. Interacts (via the TSP type I repeats) with CD36; the interaction conveys an antiangiogenic effect. Interacts (via the TSP type I repeats) with HRG; the interaction blocks the antiangiogenic effect of THBS1 with CD36 By similarity. Interacts with ATF6 (via lumenal domain) By similarity.

Protein-protein interaction databases

IntActiQ28178. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ28178.
SMRiQ28178. Positions 25-233, 434-546, 549-1169.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini65 – 270206Laminin G-like
Add
BLAST
Domaini316 – 37358VWFC
Add
BLAST
Domaini379 – 42951TSP type-1 1
Add
BLAST
Domaini435 – 49056TSP type-1 2
Add
BLAST
Domaini492 – 54756TSP type-1 3
Add
BLAST
Domaini547 – 58741EGF-like 1
Add
BLAST
Domaini646 – 69045EGF-like 2
Add
BLAST
Repeati691 – 72636TSP type-3 1
Add
BLAST
Repeati727 – 76236TSP type-3 2
Add
BLAST
Repeati763 – 78523TSP type-3 3
Add
BLAST
Repeati786 – 82136TSP type-3 4
Add
BLAST
Repeati822 – 84423TSP type-3 5
Add
BLAST
Repeati845 – 88238TSP type-3 6
Add
BLAST
Repeati883 – 91836TSP type-3 7
Add
BLAST
Repeati919 – 95436TSP type-3 8
Add
BLAST
Domaini958 – 1170213TSP C-terminal
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni47 – 9549Heparin-binding By similarity
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi926 – 9283Cell attachment site Reviewed prediction

Sequence similaritiesi

Belongs to the thrombospondin family.
Contains 2 EGF-like domains.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain.

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiNOG12793.
HOGENOMiHOG000007542.
HOVERGENiHBG018006.
InParanoidiQ28178.
KOiK16857.

Family and domain databases

Gene3Di2.60.120.200. 2 hits.
4.10.1080.10. 2 hits.
InterProiIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR001791. Laminin_G.
IPR028499. Thrombospondin-1.
IPR000884. Thrombospondin_1_rpt.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
IPR001007. VWF_C.
[Graphical view]
PANTHERiPTHR10199:SF78. PTHR10199:SF78. 1 hit.
PfamiPF07645. EGF_CA. 1 hit.
PF00090. TSP_1. 3 hits.
PF02412. TSP_3. 7 hits.
PF05735. TSP_C. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 3 hits.
SM00209. TSP1. 3 hits.
SM00210. TSPN. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
SSF82895. SSF82895. 3 hits.
PROSITEiPS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS50092. TSP1. 3 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q28178-1 [UniParc]FASTAAdd to Basket

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MGLAWGLGVL LLLHACGSNR IPESGGDNSV FDIFELTGAA RKRSGRRLVK     50
GPDPSSPAFR IEDANLIPPV PDKKFQDLVD AVRAEKGFLL LASLRQMKKT 100
RGTLLAVERK DHSGQVFSVI SNGKAGTLDL SLTVQGKQHV VSVEEALLAT 150
GQWKSITLFV QEDRAQLYID CEKMENAELD VPIQSIFTRD LASIARLRIA 200
KGGVNDNFQG VLQNVRFVFG TTPEDILRNK GCSSSTSVFV TLDNNVVNGS 250
SPAIRTDYIG HKTKDLQAIC GISCDELSSM VLELRGLRTI VTTLQDSIRK 300
VTEENKELAN ELRRPPLCYH NGVQYRTGDE WTVDSCTECR CQNSVTICKK 350
VSCPIMPCSN ATVPDGECCP RCWPSDSADD GWSPWSEWTS CSVTCGNGIQ 400
QRGRSCDSLN NRCEGSSVQT RTCHIQECDK RFKQDGGWSH WSPWSSCSVT 450
CGDGVITRIR LCNSPSPQMN GKPCEGKARE TKACQKDSCP INGGWGPWSP 500
WDICSVTCGG GVQKRSRLCN NPKPQFGGKD CVGDVTENQI CNKQDCPIDG 550
CLSNPCFAGV QCTSYPDGSW KCGACPPGYS GDGVECKDVD ECKEVPDACF 600
NHNGEHRCEN TDPGYNCLPC PPRFTGSQPF GRGVEHATAN KQVCKPRNPC 650
TDGTHDCNKN AKCNYLGHYS DPMYRCECKP GYAGNGIICG EDTDLDGWPN 700
EDLLCVANAT YHCRKDNCPN LPNSGQEDYD KDGIGDACDD DDDNDKIPDD 750
RDNCPFHYNP AQYDYDRDDV GDRCDNCPYN HNPDQADTDN NGEGDACAAD 800
IDGDSILNER DNCQYVYNVD QKDTDMDGVG DQCDNCPLEH NPDQLDSDSD 850
RIGDTCDNNQ DIDEDGHQNN LDNCPYVPNA NQADHDKDGK GDACDHDDDN 900
DGIPDDRDNC RLVPNPDQKD SDGDGRGDAC KDDFDQDKVP DIDDICPENV 950
DISETDFRRF QMIPLDPKGT SQNDPNWVVR HQGKELVQTV NCDPGLAVGY 1000
DEFNAVDFSG TFFINTERDD DYAGFVFGYQ SSSRFYVVMW KQVTQSYWDT 1050
NPTRAQGYSG LSVKVVNSTT GPGEHLRNAL WHTGNTSGQV RTLWHDPRHI 1100
GWKDFTAYRW HLSHRPKTGF IRVVMYEGKK IMADSGPIYD KTYAGGRLGL 1150
FVFSQEMVFF SDLKYECRDS 1170
Length:1,170
Mass (Da):129,534
Last modified:December 1, 2000 - v2
Checksum:i0DD6ADF3E5FA031A
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti805 – 8051S → G in CAA60950. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB005287 mRNA. Translation: BAA21115.1.
X87618 mRNA. Translation: CAA60950.1.
X87619 mRNA. Translation: CAA60951.1.
PIRiS55501.
RefSeqiNP_776621.1. NM_174196.1.
UniGeneiBt.109568.

Genome annotation databases

GeneIDi281530.
KEGGibta:281530.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB005287 mRNA. Translation: BAA21115.1 .
X87618 mRNA. Translation: CAA60950.1 .
X87619 mRNA. Translation: CAA60951.1 .
PIRi S55501.
RefSeqi NP_776621.1. NM_174196.1.
UniGenei Bt.109568.

3D structure databases

ProteinModelPortali Q28178.
SMRi Q28178. Positions 25-233, 434-546, 549-1169.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q28178. 1 interaction.

PTM databases

UniCarbKBi Q28178.

Proteomic databases

PaxDbi Q28178.
PRIDEi Q28178.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 281530.
KEGGi bta:281530.

Organism-specific databases

CTDi 7057.

Phylogenomic databases

eggNOGi NOG12793.
HOGENOMi HOG000007542.
HOVERGENi HBG018006.
InParanoidi Q28178.
KOi K16857.

Miscellaneous databases

NextBioi 20805487.

Family and domain databases

Gene3Di 2.60.120.200. 2 hits.
4.10.1080.10. 2 hits.
InterProi IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR001791. Laminin_G.
IPR028499. Thrombospondin-1.
IPR000884. Thrombospondin_1_rpt.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
IPR001007. VWF_C.
[Graphical view ]
PANTHERi PTHR10199:SF78. PTHR10199:SF78. 1 hit.
Pfami PF07645. EGF_CA. 1 hit.
PF00090. TSP_1. 3 hits.
PF02412. TSP_3. 7 hits.
PF05735. TSP_C. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view ]
SMARTi SM00181. EGF. 3 hits.
SM00209. TSP1. 3 hits.
SM00210. TSPN. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view ]
SUPFAMi SSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
SSF82895. SSF82895. 3 hits.
PROSITEi PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS50092. TSP1. 3 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "cDNA cloning of bovine thrombospondin 1 and its expression in odontoblasts and predentin."
    Ueno A., Yamashita K., Nagata T., Tsurumi C., Miwa Y., Kitamura S., Inoue H.
    Biochim. Biophys. Acta 1382:17-22(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], POSSIBLE FUNCTION, TISSUE SPECIFICITY.
    Strain: Holstein.
    Tissue: Tooth.
  2. "Cloning and sequencing of bovine thrombospondin stimulatory effect of TGF-beta."
    Zafar R.S., Moll Y.D., Womack J.F., Walz D.A.
    Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-18 AND 710-1170.
    Tissue: Aortic endothelium.

Entry informationi

Entry nameiTSP1_BOVIN
AccessioniPrimary (citable) accession number: Q28178
Secondary accession number(s): Q28179
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 1, 2000
Last modified: September 3, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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