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Q28178

- TSP1_BOVIN

UniProt

Q28178 - TSP1_BOVIN

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Protein

Thrombospondin-1

Gene

THBS1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties (By similarity). May play a role in dentinogenesis and/or maintenance of dentin and dental pulp. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity).By similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. extracellular matrix binding Source: InterPro
  3. heparin binding Source: UniProtKB-KW

GO - Biological processi

  1. behavioral response to pain Source: UniProtKB
  2. cell adhesion Source: UniProtKB-KW
  3. inflammatory response Source: InterPro
  4. negative regulation of angiogenesis Source: InterPro
  5. response to endoplasmic reticulum stress Source: UniProtKB
  6. response to unfolded protein Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell adhesion, Unfolded protein response

Keywords - Ligandi

Calcium, Heparin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombospondin-1
Gene namesi
Name:THBS1
Synonyms:TSP-1, TSP1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Endoplasmic reticulum By similarity. Sarcoplasmic reticulum By similarity

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB
  2. extracellular space Source: InterPro
  3. sarcoplasmic reticulum Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818By similarityAdd
BLAST
Chaini19 – 11701152Thrombospondin-1PRO_0000035841Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi171 ↔ 232By similarity
Glycosylationi248 – 2481N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi270 – 270InterchainCurated
Disulfide bondi274 – 274InterchainCurated
Glycosylationi360 – 3601N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi391 ↔ 423By similarity
Disulfide bondi395 ↔ 428By similarity
Disulfide bondi406 ↔ 413By similarity
Disulfide bondi447 ↔ 484By similarity
Disulfide bondi451 ↔ 489By similarity
Disulfide bondi462 ↔ 474By similarity
Disulfide bondi504 ↔ 541By similarity
Disulfide bondi508 ↔ 546By similarity
Disulfide bondi519 ↔ 531By similarity
Disulfide bondi551 ↔ 562By similarity
Disulfide bondi556 ↔ 572By similarity
Disulfide bondi575 ↔ 586By similarity
Disulfide bondi592 ↔ 608By similarity
Disulfide bondi599 ↔ 617By similarity
Disulfide bondi620 ↔ 644By similarity
Disulfide bondi650 ↔ 663By similarity
Disulfide bondi657 ↔ 676By similarity
Disulfide bondi678 ↔ 689By similarity
Disulfide bondi705 ↔ 713By similarity
Glycosylationi708 – 7081N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi718 ↔ 738By similarity
Disulfide bondi754 ↔ 774By similarity
Disulfide bondi777 ↔ 797By similarity
Disulfide bondi813 ↔ 833By similarity
Disulfide bondi836 ↔ 856By similarity
Disulfide bondi874 ↔ 894By similarity
Disulfide bondi910 ↔ 930By similarity
Disulfide bondi946 ↔ 1167By similarity
Glycosylationi1067 – 10671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1085 – 10851N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ28178.
PRIDEiQ28178.

PTM databases

UniCarbKBiQ28178.

Expressioni

Tissue specificityi

Odontoblasts.1 Publication

Interactioni

Subunit structurei

Homotrimer; disulfide-linked (By similarity). Can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1, alpha-V/beta-3 and alpha-IIb/beta-3. Binds heparin. Interacts (via the TSP type I repeats) with CD36; the interaction conveys an antiangiogenic effect. Interacts (via the TSP type I repeats) with HRG; the interaction blocks the antiangiogenic effect of THBS1 with CD36 (By similarity). Interacts with ATF6 (via lumenal domain) (By similarity).By similarity

Protein-protein interaction databases

IntActiQ28178. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ28178.
SMRiQ28178. Positions 25-233, 434-546, 549-1169.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini65 – 270206Laminin G-likeAdd
BLAST
Domaini316 – 37358VWFCPROSITE-ProRule annotationAdd
BLAST
Domaini379 – 42951TSP type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini435 – 49056TSP type-1 2PROSITE-ProRule annotationAdd
BLAST
Domaini492 – 54756TSP type-1 3PROSITE-ProRule annotationAdd
BLAST
Domaini547 – 58741EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini646 – 69045EGF-like 2PROSITE-ProRule annotationAdd
BLAST
Repeati691 – 72636TSP type-3 1Add
BLAST
Repeati727 – 76236TSP type-3 2Add
BLAST
Repeati763 – 78523TSP type-3 3Add
BLAST
Repeati786 – 82136TSP type-3 4Add
BLAST
Repeati822 – 84423TSP type-3 5Add
BLAST
Repeati845 – 88238TSP type-3 6Add
BLAST
Repeati883 – 91836TSP type-3 7Add
BLAST
Repeati919 – 95436TSP type-3 8Add
BLAST
Domaini958 – 1170213TSP C-terminalPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni47 – 9549Heparin-bindingBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi926 – 9283Cell attachment siteSequence Analysis

Sequence similaritiesi

Belongs to the thrombospondin family.Curated
Contains 2 EGF-like domains.PROSITE-ProRule annotation
Contains 1 laminin G-like domain.Curated
Contains 1 TSP C-terminal (TSPC) domain.PROSITE-ProRule annotation
Contains 3 TSP type-1 domains.PROSITE-ProRule annotation
Contains 8 TSP type-3 repeats.PROSITE-ProRule annotation
Contains 1 VWFC domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiNOG12793.
HOGENOMiHOG000007542.
HOVERGENiHBG018006.
InParanoidiQ28178.
KOiK16857.

Family and domain databases

Gene3Di2.60.120.200. 2 hits.
4.10.1080.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR001791. Laminin_G.
IPR028499. Thrombospondin-1.
IPR000884. Thrombospondin_1_rpt.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
IPR001007. VWF_C.
[Graphical view]
PANTHERiPTHR10199:SF78. PTHR10199:SF78. 1 hit.
PfamiPF07645. EGF_CA. 1 hit.
PF00090. TSP_1. 3 hits.
PF02412. TSP_3. 7 hits.
PF05735. TSP_C. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
SMARTiSM00181. EGF. 3 hits.
SM00209. TSP1. 3 hits.
SM00210. TSPN. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMiSSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
SSF82895. SSF82895. 3 hits.
PROSITEiPS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS50092. TSP1. 3 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q28178-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGLAWGLGVL LLLHACGSNR IPESGGDNSV FDIFELTGAA RKRSGRRLVK
60 70 80 90 100
GPDPSSPAFR IEDANLIPPV PDKKFQDLVD AVRAEKGFLL LASLRQMKKT
110 120 130 140 150
RGTLLAVERK DHSGQVFSVI SNGKAGTLDL SLTVQGKQHV VSVEEALLAT
160 170 180 190 200
GQWKSITLFV QEDRAQLYID CEKMENAELD VPIQSIFTRD LASIARLRIA
210 220 230 240 250
KGGVNDNFQG VLQNVRFVFG TTPEDILRNK GCSSSTSVFV TLDNNVVNGS
260 270 280 290 300
SPAIRTDYIG HKTKDLQAIC GISCDELSSM VLELRGLRTI VTTLQDSIRK
310 320 330 340 350
VTEENKELAN ELRRPPLCYH NGVQYRTGDE WTVDSCTECR CQNSVTICKK
360 370 380 390 400
VSCPIMPCSN ATVPDGECCP RCWPSDSADD GWSPWSEWTS CSVTCGNGIQ
410 420 430 440 450
QRGRSCDSLN NRCEGSSVQT RTCHIQECDK RFKQDGGWSH WSPWSSCSVT
460 470 480 490 500
CGDGVITRIR LCNSPSPQMN GKPCEGKARE TKACQKDSCP INGGWGPWSP
510 520 530 540 550
WDICSVTCGG GVQKRSRLCN NPKPQFGGKD CVGDVTENQI CNKQDCPIDG
560 570 580 590 600
CLSNPCFAGV QCTSYPDGSW KCGACPPGYS GDGVECKDVD ECKEVPDACF
610 620 630 640 650
NHNGEHRCEN TDPGYNCLPC PPRFTGSQPF GRGVEHATAN KQVCKPRNPC
660 670 680 690 700
TDGTHDCNKN AKCNYLGHYS DPMYRCECKP GYAGNGIICG EDTDLDGWPN
710 720 730 740 750
EDLLCVANAT YHCRKDNCPN LPNSGQEDYD KDGIGDACDD DDDNDKIPDD
760 770 780 790 800
RDNCPFHYNP AQYDYDRDDV GDRCDNCPYN HNPDQADTDN NGEGDACAAD
810 820 830 840 850
IDGDSILNER DNCQYVYNVD QKDTDMDGVG DQCDNCPLEH NPDQLDSDSD
860 870 880 890 900
RIGDTCDNNQ DIDEDGHQNN LDNCPYVPNA NQADHDKDGK GDACDHDDDN
910 920 930 940 950
DGIPDDRDNC RLVPNPDQKD SDGDGRGDAC KDDFDQDKVP DIDDICPENV
960 970 980 990 1000
DISETDFRRF QMIPLDPKGT SQNDPNWVVR HQGKELVQTV NCDPGLAVGY
1010 1020 1030 1040 1050
DEFNAVDFSG TFFINTERDD DYAGFVFGYQ SSSRFYVVMW KQVTQSYWDT
1060 1070 1080 1090 1100
NPTRAQGYSG LSVKVVNSTT GPGEHLRNAL WHTGNTSGQV RTLWHDPRHI
1110 1120 1130 1140 1150
GWKDFTAYRW HLSHRPKTGF IRVVMYEGKK IMADSGPIYD KTYAGGRLGL
1160 1170
FVFSQEMVFF SDLKYECRDS
Length:1,170
Mass (Da):129,534
Last modified:December 1, 2000 - v2
Checksum:i0DD6ADF3E5FA031A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti805 – 8051S → G in CAA60950. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB005287 mRNA. Translation: BAA21115.1.
X87618 mRNA. Translation: CAA60950.1.
X87619 mRNA. Translation: CAA60951.1.
PIRiS55501.
RefSeqiNP_776621.1. NM_174196.1.
UniGeneiBt.109568.

Genome annotation databases

GeneIDi281530.
KEGGibta:281530.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB005287 mRNA. Translation: BAA21115.1 .
X87618 mRNA. Translation: CAA60950.1 .
X87619 mRNA. Translation: CAA60951.1 .
PIRi S55501.
RefSeqi NP_776621.1. NM_174196.1.
UniGenei Bt.109568.

3D structure databases

ProteinModelPortali Q28178.
SMRi Q28178. Positions 25-233, 434-546, 549-1169.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q28178. 1 interaction.

PTM databases

UniCarbKBi Q28178.

Proteomic databases

PaxDbi Q28178.
PRIDEi Q28178.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 281530.
KEGGi bta:281530.

Organism-specific databases

CTDi 7057.

Phylogenomic databases

eggNOGi NOG12793.
HOGENOMi HOG000007542.
HOVERGENi HBG018006.
InParanoidi Q28178.
KOi K16857.

Miscellaneous databases

NextBioi 20805487.

Family and domain databases

Gene3Di 2.60.120.200. 2 hits.
4.10.1080.10. 2 hits.
InterProi IPR013320. ConA-like_dom.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR001791. Laminin_G.
IPR028499. Thrombospondin-1.
IPR000884. Thrombospondin_1_rpt.
IPR003367. Thrombospondin_3-like_rpt.
IPR017897. Thrombospondin_3_rpt.
IPR008859. Thrombospondin_C.
IPR028974. TSP_type-3_rpt.
IPR001007. VWF_C.
[Graphical view ]
PANTHERi PTHR10199:SF78. PTHR10199:SF78. 1 hit.
Pfami PF07645. EGF_CA. 1 hit.
PF00090. TSP_1. 3 hits.
PF02412. TSP_3. 7 hits.
PF05735. TSP_C. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view ]
SMARTi SM00181. EGF. 3 hits.
SM00209. TSP1. 3 hits.
SM00210. TSPN. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view ]
SUPFAMi SSF103647. SSF103647. 3 hits.
SSF49899. SSF49899. 2 hits.
SSF82895. SSF82895. 3 hits.
PROSITEi PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 2 hits.
PS50092. TSP1. 3 hits.
PS51234. TSP3. 8 hits.
PS51236. TSP_CTER. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "cDNA cloning of bovine thrombospondin 1 and its expression in odontoblasts and predentin."
    Ueno A., Yamashita K., Nagata T., Tsurumi C., Miwa Y., Kitamura S., Inoue H.
    Biochim. Biophys. Acta 1382:17-22(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], POSSIBLE FUNCTION, TISSUE SPECIFICITY.
    Strain: Holstein.
    Tissue: Tooth.
  2. "Cloning and sequencing of bovine thrombospondin stimulatory effect of TGF-beta."
    Zafar R.S., Moll Y.D., Womack J.F., Walz D.A.
    Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-18 AND 710-1170.
    Tissue: Aortic endothelium.

Entry informationi

Entry nameiTSP1_BOVIN
AccessioniPrimary (citable) accession number: Q28178
Secondary accession number(s): Q28179
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 1, 2000
Last modified: October 29, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3