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Protein

Phakinin

Gene

BFSP2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in stabilization of lens fiber cell cytoskeleton.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Eye lens protein

Names & Taxonomyi

Protein namesi
Recommended name:
Phakinin
Alternative name(s):
49 kDa cytoskeletal protein
Beaded filament structural protein 2
Lens fiber cell beaded filament protein CP 47
Short name:
CP47
Lens fiber cell beaded filament protein CP 49
Short name:
CP49
Gene namesi
Name:BFSP2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

  • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
  • Cytoplasmcytoskeleton 1 Publication
  • Cytoplasmcell cortex 1 Publication

  • Note: Detected adjacent to the cell membrane.1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Intermediate filament, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 415415PhakininPRO_0000063850Add
BLAST

Proteomic databases

PRIDEiQ28177.

Expressioni

Tissue specificityi

Detected in eye lens fiber cells (at protein level).1 Publication

Interactioni

Subunit structurei

Associates with BFSP1 (PubMed:7504675). Interacts with LGSN. Identified in complexes that contain VIM, EZR, AHNAK, BFSP1, BFSP2, ANK2, PLEC, PRX and spectrin (By similarity).By similarity1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ28177.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 114114HeadAdd
BLAST
Regioni115 – 395281RodAdd
BLAST
Regioni396 – 41520TailAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili115 – 14430Sequence analysisAdd
BLAST
Coiled coili199 – 24850Sequence analysisAdd
BLAST
Coiled coili295 – 395101Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the intermediate filament family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

HOGENOMiHOG000230975.
HOVERGENiHBG013015.
InParanoidiQ28177.
KOiK10379.

Family and domain databases

InterProiIPR001664. IF.
IPR002957. Keratin_I.
IPR027694. Phakinin.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PTHR23239:SF32. PTHR23239:SF32. 1 hit.
PfamiPF00038. Filament. 1 hit.
[Graphical view]
PRINTSiPR01248. TYPE1KERATIN.
SMARTiSM01391. Filament. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q28177-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTRRVVVDA PAGASSSMPL QRHKASFRAA QSPSSLDGLP ASRTVAVSGL
60 70 80 90 100
VRTPRVYVGM APSGPTGGLG ARVTRRALGI SSVFLQGLRS SGLATAPAPS
110 120 130 140 150
LERDLGAVED LGGCLVEYMA KVHALEKVSQ ELEAQLRMHL ESKATRSENW
160 170 180 190 200
GALRASWASS CQQVGEAVLE NARLMLQTEN IQAGADDFKE RYENEQPFRK
210 220 230 240 250
AAEEEINSLY KVIDEANSSK MDLESQIESL KEELGFLSRS YEEDVKMLYK
260 270 280 290 300
QLAGSELEQL NVPIGTGLDD ILETIRIHWE RDVEKNRLQA GALLQAKQQA
310 320 330 340 350
ELARRAQTQE EKLAAALRVE LHNTSCQIQS LQAETESLRA LKRGLENTLH
360 370 380 390 400
DAKHWHDIEL QNLGAVVSRL EAELREMRAE AEQQLQAREH LLSHKCQLQR
410
DVASYHALLD REESS
Length:415
Mass (Da):45,950
Last modified:November 1, 1996 - v1
Checksum:iF3996852E69661B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75160 mRNA. Translation: CAA53003.1.
BC122762 mRNA. Translation: AAI22763.1.
RefSeqiNP_776673.1. NM_174248.3.
UniGeneiBt.320.

Genome annotation databases

GeneIDi281645.
KEGGibta:281645.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75160 mRNA. Translation: CAA53003.1.
BC122762 mRNA. Translation: AAI22763.1.
RefSeqiNP_776673.1. NM_174248.3.
UniGeneiBt.320.

3D structure databases

ProteinModelPortaliQ28177.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ28177.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281645.
KEGGibta:281645.

Organism-specific databases

CTDi8419.

Phylogenomic databases

HOGENOMiHOG000230975.
HOVERGENiHBG013015.
InParanoidiQ28177.
KOiK10379.

Miscellaneous databases

NextBioi20805578.

Family and domain databases

InterProiIPR001664. IF.
IPR002957. Keratin_I.
IPR027694. Phakinin.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PTHR23239:SF32. PTHR23239:SF32. 1 hit.
PfamiPF00038. Filament. 1 hit.
[Graphical view]
PRINTSiPR01248. TYPE1KERATIN.
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The 47-kD lens-specific protein phakinin is a tailless intermediate filament protein and an assembly partner of filensin."
    Merdes A., Gounari F., Georgatos S.D.
    J. Cell Biol. 123:1507-1516(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH BFSP1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Lens.
  2. Merdes A.
    Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Hypothalamus.
  4. "cDNA analysis of the 49 kDa lens fiber cell cytoskeletal protein: a new, lens-specific member of the intermediate filament family?"
    Hess J.F., Casselman J.T., FitzGerald P.G.
    Curr. Eye Res. 12:77-88(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 222-232 AND 248-277.
    Tissue: Lens.

Entry informationi

Entry nameiBFSP2_BOVIN
AccessioniPrimary (citable) accession number: Q28177
Secondary accession number(s): Q05B61
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.