Q28153 (CELA1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chymotrypsin-like elastase family member 1
EC=3.4.21.36

Alternative name(s):
Elastase I
Elastase-1

Gene names

Name:	CELA1
Synonyms:	ELA1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts upon elastin.
Catalytic activity	Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-Xaa.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted.
Tissue specificity	Pancreas.
Sequence similarities	Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	By similarity
	Propeptide	17 – 26	10	Activation peptide By similarity		PRO_0000027671
	Chain	27 – 266	240	Chymotrypsin-like elastase family member 1		PRO_0000027672
Regions
	Domain	27 – 264	238	Peptidase S1
Sites
	Active site	71	1	Charge relay system By similarity
	Active site	119	1	Charge relay system By similarity
	Active site	214	1	Charge relay system By similarity
	Metal binding	85	1	Calcium By similarity
	Metal binding	90	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	95	1	Calcium By similarity
Amino acid modifications
	Glycosylation	87	1	N-linked (GlcNAc...) Potential
	Glycosylation	241	1	N-linked (GlcNAc...) Potential
	Disulfide bond	56 ↔ 72		By similarity
	Disulfide bond	153 ↔ 220		By similarity
	Disulfide bond	184 ↔ 200		By similarity
	Disulfide bond	210 ↔ 240		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q28153 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 927E29C69BAF67E7	FASTA	266	28,518
	10 20 30 40 50 60 MLRLLVFTSL VLYGHSTQDF PETNARVVGG TAVSKNSWPS QISLQYKSGS SWYHTCGGTL 70 80 90 100 110 120 IKQKWVMTAA HCVDSQMTFR VVLGDHNLSQ NDGTEQYISV QKIVVHPSWN SNNVAAGYDI 130 140 150 160 170 180 AVLRLAQSAT LNSYVQLGVL PQSGTILANN TPCYITGWGR TKTNGQLAQT LQQAYLPSVD 190 200 210 220 230 240 YATCSSSSYW GSTVKTTMVC AGGDGVRAGC QGDSGGPLHC LVNGQYAVHG VTSFVSSLGC 250 260 NVSKKPTVFT RVSAYISWIN NAIASN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bovine pancreatic preproelastases I and II: comparison of nucleotide and amino acid sequences and tissue specific expression." Gestin M., le Huerou-Luron I., Wicker-Planquart C., le Drean G., Chaix J.-C., Puigserver A., Guilloteau P. Comp. Biochem. Physiol. 118B:181-187(1997) [PubMed: 9418008] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Holstein-Friesian. Tissue: Pancreas.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal pancreas.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M80838 mRNA. Translation: AAA98525.1. BC149525 mRNA. Translation: AAI49526.1.
IPI	IPI00688976.
RefSeq	NP_776473.1. NM_174048.1.
UniGene	Bt.4522.
3D structure databases
ProteinModelPortal	Q28153.
SMR	Q28153. Positions 27-266.
ModBase	Search...
Protein-protein interaction databases
STRING	Q28153.
Protein family/group databases
MEROPS	S01.153.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000011718; ENSBTAP00000011718; ENSBTAG00000008900.
GeneID	281139.
KEGG	bta:281139.
Organism-specific databases
CTD	1990.
Phylogenomic databases
eggNOG	maNOG15434.
GeneTree	ENSGT00550000074117.
InParanoid	Q28153.
OMA	PSVDYAT.
OrthoDB	EOG4V171P.
PhylomeDB	Q28153.
Family and domain databases
InterPro	IPR009003. Pept_cys/ser_Trypsin-like. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
KO	K01326.
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CELA1_BOVIN

Accession

Primary (citable) accession number: Q28153
Secondary accession number(s): A6QPW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 16, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program