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Protein

Nuclear inhibitor of protein phosphatase 1

Gene

PPP1R8

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protein phosphatase inhibitor, Repressor

Keywords - Biological processi

mRNA processing, mRNA splicing, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear inhibitor of protein phosphatase 1
Short name:
NIPP-1
Alternative name(s):
Protein phosphatase 1 regulatory inhibitor subunit 8
Gene namesi
Name:PPP1R8
Synonyms:NIPP1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 351351Nuclear inhibitor of protein phosphatase 1PRO_0000071504Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei161 – 1611Phosphothreonine; by CK2; in vitro1 Publication
Modified residuei178 – 1781Phosphoserine; by PKA; in vitro1 Publication
Modified residuei199 – 1991Phosphoserine2 Publications
Modified residuei204 – 2041Phosphoserine2 Publications
Modified residuei249 – 2491PhosphoserineBy similarity
Modified residuei264 – 2641PhosphotyrosineBy similarity

Post-translational modificationi

The N-terminus is blocked.
Inactivated by phosphorylation on Ser-199 or Ser-204.3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei202 – 2021Not phosphorylatedCurated
Sitei346 – 3461Not phosphorylatedCurated

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ28147.
PRIDEiQ28147.

PTM databases

iPTMnetiQ28147.

Interactioni

Subunit structurei

Interacts with phosphorylated CDC5L, SF3B1 and MELK. Interacts with EED. Part of a complex consisting of PPP1R8, EED, HDAC2 and PP-1. Part of the spliceosome. Interacts with PPP1CA, PPP1CB and PPP1CC (By similarity).By similarity

Protein-protein interaction databases

BioGridi159581. 4 interactions.
DIPiDIP-438N.
STRINGi9913.ENSBTAP00000005043.

Structurei

3D structure databases

ProteinModelPortaliQ28147.
SMRiQ28147. Positions 1-132.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini49 – 10153FHAPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 142142Interaction with CDC5L, SF3B1 and MELKBy similarityAdd
BLAST
Regioni143 – 22482Interaction with EEDBy similarityAdd
BLAST
Regioni191 – 20010Involved in PP-1 inhibition
Regioni200 – 2034Involved in PP-1 binding
Regioni310 – 32920Interaction with EEDBy similarityAdd
BLAST
Regioni330 – 35122RNA-bindingBy similarityAdd
BLAST
Regioni331 – 3377Involved in PP-1 inhibitionBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi185 – 20925Nuclear localization signal 1By similarityAdd
BLAST
Motifi210 – 24031Nuclear localization signal 2By similarityAdd
BLAST

Domaini

Has a basic N- and C-terminal and an acidic central domain.1 Publication
The FHA domain mediates interactions with threonine-phosphorylated MELK.By similarity

Sequence similaritiesi

Contains 1 FHA domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1880. Eukaryota.
ENOG410XTHZ. LUCA.
HOGENOMiHOG000231315.
HOVERGENiHBG053645.
InParanoidiQ28147.
KOiK13216.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
InterProiIPR000253. FHA_dom.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PfamiPF00498. FHA. 1 hit.
[Graphical view]
SMARTiSM00240. FHA. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: Q28147-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAANSGSS LPLFDCPTWA GKPPPGLHLD VVKGDKLIEK LIIDEKKYYL
60 70 80 90 100
FGRNPDLCDF TIDHQSCSRV HAALVYHKHL KRVFLIDLNS THGTFLGHIR
110 120 130 140 150
LEPHKPQQIP IDSTVSFGAS TRAYTLREKP QTLPSAVKGD EKMGGEDDEL
160 170 180 190 200
KGLLGLPEEE TELDNLTEFN TAHNKRISTL TIEEGNLDIQ RPKRKRKNSR
210 220 230 240 250
VTFSEDDEII NPEDVDPSVG RFRNMVQTAV VPVKKKRVEG PGSLVLEESG
260 270 280 290 300
SRRMQNFAFS GGLYGGLPPT HSEAGSQPHG IHGTALIGGL PMPYPNLAPD
310 320 330 340 350
VDLTPVVPSA VNMNPAPNPA VYNPEAVNEP KKKKYAKEAW PGKKPTPSLL

I
Length:351
Mass (Da):38,521
Last modified:November 1, 1996 - v1
Checksum:i7CD5F6E162A66210
GO
Isoform B (identifier: Q28147-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-142: Missing.

Show »
Length:209
Mass (Da):22,753
Checksum:iCE73F2484B0FA477
GO

Mass spectrometryi

Molecular mass is 38520 Da from positions 1 - 351. Determined by ESI. Expressed in baculovirus.1 Publication

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 142142Missing in isoform B. CuratedVSP_005118Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z50748 mRNA. Translation: CAA90625.1.
PIRiI46033.
RefSeqiNP_777007.1. NM_174582.2. [Q28147-1]
UniGeneiBt.22.

Genome annotation databases

GeneIDi282319.
KEGGibta:282319.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z50748 mRNA. Translation: CAA90625.1.
PIRiI46033.
RefSeqiNP_777007.1. NM_174582.2. [Q28147-1]
UniGeneiBt.22.

3D structure databases

ProteinModelPortaliQ28147.
SMRiQ28147. Positions 1-132.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi159581. 4 interactions.
DIPiDIP-438N.
STRINGi9913.ENSBTAP00000005043.

PTM databases

iPTMnetiQ28147.

Proteomic databases

PaxDbiQ28147.
PRIDEiQ28147.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi282319.
KEGGibta:282319.

Organism-specific databases

CTDi5511.

Phylogenomic databases

eggNOGiKOG1880. Eukaryota.
ENOG410XTHZ. LUCA.
HOGENOMiHOG000231315.
HOVERGENiHBG053645.
InParanoidiQ28147.
KOiK13216.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
InterProiIPR000253. FHA_dom.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PfamiPF00498. FHA. 1 hit.
[Graphical view]
SMARTiSM00240. FHA. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPP1R8_BOVIN
AccessioniPrimary (citable) accession number: Q28147
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

A synthetic peptide, NIPP-1(191-200), is able to inhibit PP-1. Alanine substitution of Val-201 and Phe-203 in NIPP-1(191-210) prevents PP-1 binding (far-western assay) but do not affect PP-1 inhibition. Phosphorylation of Ser-199 or Ser-204 prevents PP-1 binding (far-western assay) and reduces PP-1 inhibition.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.