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Protein

Sodium/potassium/calcium exchanger 1

Gene

SLC24A1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Critical component of the visual transduction cascade, controlling the calcium concentration of outer segments during light and darkness. Light causes a rapid lowering of cytosolic free calcium in the outer segment of both retinal rod and cone photoreceptors and the light-induced lowering of calcium is caused by extrusion via this protein which plays a key role in the process of light adaptation. Transports 1 Ca2+ and 1 K+ in exchange for 4 Na+ (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Antiport, Calcium transport, Ion transport, Potassium transport, Sensory transduction, Sodium transport, Symport, Transport, Vision

Keywords - Ligandi

Calcium, Potassium, Sodium

Enzyme and pathway databases

ReactomeiR-BTA-2485179. Activation of the phototransduction cascade.
R-BTA-425561. Sodium/Calcium exchangers.

Protein family/group databases

TCDBi2.A.19.4.1. the ca(2+):cation antiporter (caca) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/potassium/calcium exchanger 1
Alternative name(s):
Na(+)/K(+)/Ca(2+)-exchange protein 1
Retinal rod Na-Ca+K exchanger
Solute carrier family 24 member 1
Gene namesi
Name:SLC24A1
Synonyms:NCKX1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 10

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 446ExtracellularSequence analysisAdd BLAST446
Transmembranei447 – 467HelicalSequence analysisAdd BLAST21
Topological domaini468 – 491CytoplasmicSequence analysisAdd BLAST24
Transmembranei492 – 512HelicalSequence analysisAdd BLAST21
Topological domaini513 – 518ExtracellularSequence analysis6
Transmembranei519 – 539HelicalSequence analysisAdd BLAST21
Topological domaini540 – 557CytoplasmicSequence analysisAdd BLAST18
Transmembranei558 – 578HelicalSequence analysisAdd BLAST21
Topological domaini579ExtracellularSequence analysis1
Transmembranei580 – 600HelicalSequence analysisAdd BLAST21
Topological domaini601 – 1024CytoplasmicSequence analysisAdd BLAST424
Transmembranei1025 – 1045HelicalSequence analysisAdd BLAST21
Topological domaini1046 – 1052ExtracellularSequence analysis7
Transmembranei1053 – 1073HelicalSequence analysisAdd BLAST21
Topological domaini1074 – 1088CytoplasmicSequence analysisAdd BLAST15
Transmembranei1089 – 1109HelicalSequence analysisAdd BLAST21
Topological domaini1110 – 1127ExtracellularSequence analysisAdd BLAST18
Transmembranei1128 – 1148HelicalSequence analysisAdd BLAST21
Topological domaini1149 – 1157CytoplasmicSequence analysis9
Transmembranei1158 – 1178HelicalSequence analysisAdd BLAST21
Topological domaini1179 – 1185ExtracellularSequence analysis7
Transmembranei1186 – 1206HelicalSequence analysisAdd BLAST21
Topological domaini1207 – 1216CytoplasmicSequence analysis10

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi65R → A: Does not affect the targeting. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002233021 – 1216Sodium/potassium/calcium exchanger 1Add BLAST1216
Signal peptidei1 – ?Not cleaved

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi290N-linked (GlcNAc...)Sequence analysis1
Glycosylationi303N-linked (GlcNAc...)Sequence analysis1
Modified residuei652PhosphoserineSequence analysis1
Modified residuei717PhosphothreonineBy similarity1

Post-translational modificationi

The uncleaved signal sequence is required for efficient membrane targeting and proper membrane integration and topology.
Glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ28139.
PRIDEiQ28139.

Expressioni

Tissue specificityi

Retina.

Gene expression databases

BgeeiENSBTAG00000025826.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000036386.

Structurei

3D structure databases

ProteinModelPortaliQ28139.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati488 – 528Alpha-1Add BLAST41
Repeati796 – 8111; approximateAdd BLAST16
Repeati812 – 8282Add BLAST17
Repeati829 – 8453Add BLAST17
Repeati846 – 8624Add BLAST17
Repeati863 – 8795Add BLAST17
Repeati880 – 8966Add BLAST17
Repeati897 – 9137Add BLAST17
Repeati914 – 9288; approximateAdd BLAST15
Repeati1096 – 1127Alpha-2Add BLAST32

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni796 – 9288 X 17 AA tandem repeats of D-E-D-E-G-E-I-Q-A-G-E-[GA]-G-E-V-[EK]-GAdd BLAST133

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi984 – 1009Poly-GluAdd BLAST26

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1307. Eukaryota.
ENOG410Y9YY. LUCA.
GeneTreeiENSGT00760000119209.
HOGENOMiHOG000231933.
HOVERGENiHBG104097.
InParanoidiQ28139.
KOiK13749.
OMAiDHEGETE.
OrthoDBiEOG091G0M5C.
TreeFamiTF318759.

Family and domain databases

InterProiIPR004481. K/Na/Ca-exchanger.
IPR004837. NaCa_Exmemb.
IPR004817. SLC24A1.
[Graphical view]
PANTHERiPTHR10846. PTHR10846. 4 hits.
PfamiPF01699. Na_Ca_ex. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00927. 2A1904. 2 hits.
TIGR00367. TIGR00367. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q28139-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKLIRMGAQ ERRSLWPKRL HWSRPLFLLG MLIIGSTYQY LTSPQGLPTL
60 70 80 90 100
WAAVSSQHPV KVASRDLSNK EMMMVSSETS KSSSEMEVEA WAPEATAGRD
110 120 130 140 150
GTPPGIARKN TPSTPRGTAS ITPAIPNNYS PTPTGTGKVK EDTSATPSGV
160 170 180 190 200
LNHYTQSRPM VNSYTRLTAR GEVKSSRPTQ SRGKEEKYSP SPLGRMVNSY
210 220 230 240 250
APSTLMTMPR SHGITPRTTV KDREIMATKK MLATNPSKRL VEKTTPTPLK
260 270 280 290 300
GITDNTPTFL LSDLETDTLT SPRNVVEKKT LTTPRRVDSN SSTNHQGLVG
310 320 330 340 350
KNNLTTPQGM VLEHTAAVSE GQVTISTMTR SSPTETKAST DAWKVRNPLP
360 370 380 390 400
RTSAPIIRIS SATFRGLLKN PSKAPSTPAA PRVRANPTIQ VRHCLVVEPA
410 420 430 440 450
PVAPTAPSPS WTTAVIPGIP SPSGQPDLYP KAEYPRDLFS VEERRQGWVV
460 470 480 490 500
LHIFGMMYVF VALAIVCDEY FVPALGVITD KLQISEDVAG ATFMAAGGSA
510 520 530 540 550
PELFTSLIGV FISHSNVGIG TIVGSAVFNI LFVIGTCALF SREILNLTWW
560 570 580 590 600
PLFRDITFYI FDLMMLILFF LDSLIAWWES VLLLLAYAFY VFTMKWNQQL
610 620 630 640 650
ELWVKEQLNK RPVAKVMALG DLSKPGDGTV VVDEQQDNKK LKLSSMLTRG
660 670 680 690 700
SSSASLHNST IRSTIYQLML HSLDPLGEAR PSKDKEEETL IPEAKATPQA
710 720 730 740 750
KAESKPEEEP AKLPEVTVTP APAPDVKGDQ EEDPGSQGVG AEAENTGERT
760 770 780 790 800
GGEAEAPAEG ENGERSGGDA ALGGESEGKA ENESEGDIPA ERRGDDEDEG
810 820 830 840 850
EIQAEGGEVK GDEDEGEIQA GEGGEVEGDE DEGEIQAGEG GEVEGDEDEG
860 870 880 890 900
EIQAGEAGEV EGDEDEGEIQ AGEGGEVEGD EDEGEIQAGE AGEVEGDEDE
910 920 930 940 950
GEIQAGEGGE VKGDEGEIQA GEAGEVEGED GEVEGGEDEG EIQAGEGGEG
960 970 980 990 1000
ETGEQELNAE IQGEAKDDEE GVDGEGGGDG GDSEDEEEED EEEEDEEEEE
1010 1020 1030 1040 1050
EEEEEEEEEN EQPLSLEWPE TRRKQAIYLF LLPIVFPLWL TVPDVRRLEA
1060 1070 1080 1090 1100
KKFFVITFLG SILWIAMFSY LMVWWAHQVG ETIGISEEIM GLTILAAGTS
1110 1120 1130 1140 1150
IPDLITSVIV ARKGLGDMAV SSSVGSNIFD ITVGLPLPWM LFSLINGLQP
1160 1170 1180 1190 1200
VAVSSNGLFC AIVLLFLMLL FVISSIALCK WRMNKILGFT MFLLYFVFLI
1210
ISVMLEDRII SCPVSV
Length:1,216
Mass (Da):131,614
Last modified:December 13, 2001 - v2
Checksum:i39F149A74D1D0523
GO
Isoform 2 (identifier: Q28139-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     868-884: Missing.

Note: No experimental confirmation available.
Show »
Length:1,199
Mass (Da):129,913
Checksum:i5F31F5BF1E30C451
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti84Missing AA sequence (PubMed:1582405).Curated1
Sequence conflicti234Missing AA sequence (PubMed:1582405).Curated1
Sequence conflicti244 – 245Missing AA sequence (PubMed:1582405).Curated2
Sequence conflicti857A → G in AAB88884 (PubMed:9478004).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_006159868 – 884Missing in isoform 2. 1 PublicationAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66481 mRNA. Translation: CAA47108.1.
AF025664 mRNA. Translation: AAB88884.1.
PIRiS20969.
RefSeqiNP_777080.1. NM_174655.2. [Q28139-2]
UniGeneiBt.19.

Genome annotation databases

EnsembliENSBTAT00000036529; ENSBTAP00000036386; ENSBTAG00000025826. [Q28139-2]
GeneIDi282474.
KEGGibta:282474.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66481 mRNA. Translation: CAA47108.1.
AF025664 mRNA. Translation: AAB88884.1.
PIRiS20969.
RefSeqiNP_777080.1. NM_174655.2. [Q28139-2]
UniGeneiBt.19.

3D structure databases

ProteinModelPortaliQ28139.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000036386.

Protein family/group databases

TCDBi2.A.19.4.1. the ca(2+):cation antiporter (caca) family.

Proteomic databases

PaxDbiQ28139.
PRIDEiQ28139.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000036529; ENSBTAP00000036386; ENSBTAG00000025826. [Q28139-2]
GeneIDi282474.
KEGGibta:282474.

Organism-specific databases

CTDi9187.

Phylogenomic databases

eggNOGiKOG1307. Eukaryota.
ENOG410Y9YY. LUCA.
GeneTreeiENSGT00760000119209.
HOGENOMiHOG000231933.
HOVERGENiHBG104097.
InParanoidiQ28139.
KOiK13749.
OMAiDHEGETE.
OrthoDBiEOG091G0M5C.
TreeFamiTF318759.

Enzyme and pathway databases

ReactomeiR-BTA-2485179. Activation of the phototransduction cascade.
R-BTA-425561. Sodium/Calcium exchangers.

Gene expression databases

BgeeiENSBTAG00000025826.

Family and domain databases

InterProiIPR004481. K/Na/Ca-exchanger.
IPR004837. NaCa_Exmemb.
IPR004817. SLC24A1.
[Graphical view]
PANTHERiPTHR10846. PTHR10846. 4 hits.
PfamiPF01699. Na_Ca_ex. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00927. 2A1904. 2 hits.
TIGR00367. TIGR00367. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNCKX1_BOVIN
AccessioniPrimary (citable) accession number: Q28139
Secondary accession number(s): O46384
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 13, 2001
Last sequence update: December 13, 2001
Last modified: September 7, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.