Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glial fibrillary acidic protein

Gene

GFAP

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-BTA-1251985. Nuclear signaling by ERBB4.

Names & Taxonomyi

Protein namesi
Recommended name:
Glial fibrillary acidic protein
Short name:
GFAP
Gene namesi
Name:GFAP
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 19

Subcellular locationi

  • Cytoplasm By similarity

  • Note: Associated with intermediate filaments.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Intermediate filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428Glial fibrillary acidic proteinPRO_0000063804Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei7 – 71Phosphothreonine; by AURKB and ROCK1By similarity
Modified residuei13 – 131Phosphoserine; by AURKB and ROCK1By similarity
Modified residuei26 – 261CitrullineBy similarity
Modified residuei32 – 321CitrullineBy similarity
Modified residuei34 – 341Phosphoserine; by AURKB and ROCK1By similarity
Modified residuei78 – 781PhosphoserineBy similarity
Modified residuei106 – 1061PhosphothreonineBy similarity
Modified residuei146 – 1461PhosphothreonineBy similarity
Modified residuei266 – 2661CitrullineBy similarity
Modified residuei319 – 3191PhosphoserineBy similarity
Modified residuei379 – 3791PhosphothreonineBy similarity
Modified residuei381 – 3811PhosphoserineBy similarity
Modified residuei402 – 4021CitrullineBy similarity
Modified residuei412 – 4121CitrullineBy similarity

Post-translational modificationi

Phosphorylated by PKN1.By similarity

Keywords - PTMi

Citrullination, Phosphoprotein

Proteomic databases

PaxDbiQ28115.
PRIDEiQ28115.

PTM databases

iPTMnetiQ28115.

Interactioni

Subunit structurei

Interacts with SYNM.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
PASKQ96RG22EBI-907866,EBI-1042651From a different organism.

Protein-protein interaction databases

IntActiQ28115. 2 interactions.
STRINGi9913.ENSBTAP00000017997.

Structurei

3D structure databases

ProteinModelPortaliQ28115.
SMRiQ28115. Positions 63-100, 290-368.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 6868HeadAdd
BLAST
Regioni69 – 373305RodAdd
BLAST
Regioni69 – 10032Coil 1AAdd
BLAST
Regioni101 – 11111Linker 1Add
BLAST
Regioni112 – 21099Coil 1BAdd
BLAST
Regioni211 – 22616Linker 12Add
BLAST
Regioni227 – 24822Coil 2AAdd
BLAST
Regioni249 – 2524Linker 2
Regioni253 – 373121Coil 2BAdd
BLAST
Regioni374 – 42855TailAdd
BLAST

Sequence similaritiesi

Belongs to the intermediate filament family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IJXF. Eukaryota.
ENOG410Y9QE. LUCA.
GeneTreeiENSGT00830000128228.
HOGENOMiHOG000230977.
HOVERGENiHBG013015.
InParanoidiQ28115.
KOiK05640.
OMAiHLQEYQE.
OrthoDBiEOG7FV3Q8.
TreeFamiTF330122.

Family and domain databases

InterProiIPR027701. GFAP.
IPR001664. IF.
IPR006821. Intermed_filament_DNA-bd.
IPR018039. Intermediate_filament_CS.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PTHR23239:SF41. PTHR23239:SF41. 1 hit.
PfamiPF00038. Filament. 1 hit.
PF04732. Filament_head. 1 hit.
[Graphical view]
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q28115-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERRRVTSAT RRSYVSSSEM VVGGRRLGPG TRLSLARMPP PLPARVDFSL
60 70 80 90 100
AGALNSGFKE TRASERAEMM ELNDRFASYI EKVRFLEQQN KALAAELNQL
110 120 130 140 150
RAKEPTKLAD VYQAELRELR LRLDQLTANS ARLEVERDNL AQDLGTLRQK
160 170 180 190 200
LQDETNQRLE AENNLAAYRQ EADEATLARL DLERKIESLE EEIRFLRKIH
210 220 230 240 250
EEEVRELQEQ LAQQQVHVEM DVAKPDLTAA LREIRTQYEA VASSNMHEAE
260 270 280 290 300
EWYRSKFADL NDAAARNAEL LRQAKHEAND YRRQLQALTC DLESLRGTNE
310 320 330 340 350
SLERQMREQE ERHAREAASY QEALARLEEE GQSLKDEMAR HLQEYQDLLN
360 370 380 390 400
VKLALDIEIA TYRKLLEGEE NRITIPVQTF SNLQIRETSL DTKSVSEGHL
410 420
KRNIVVKTVE MRDGEVIKES KQEHKDVM
Length:428
Mass (Da):49,512
Last modified:April 3, 2007 - v2
Checksum:i8DCCDF3E848BAD05
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti265 – 2651A → R in CAA69422 (PubMed:10571109).Curated
Sequence conflicti265 – 2651A → R in AAO18221 (Ref. 3) Curated
Sequence conflicti271 – 2711L → V in CAA69422 (PubMed:10571109).Curated
Sequence conflicti271 – 2711L → V in AAO18221 (Ref. 3) Curated
Sequence conflicti311 – 3122ER → DA in CAA69422 (PubMed:10571109).Curated
Sequence conflicti311 – 3122ER → DA in AAO18221 (Ref. 3) Curated
Sequence conflicti372 – 3721R → Q in AAO18221 (Ref. 3) Curated
Sequence conflicti421 – 4211K → P in CAA69422 (PubMed:10571109).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08255 mRNA. Translation: CAA69422.1.
BC119892 mRNA. Translation: AAI19893.1.
AY174179 Genomic DNA. Translation: AAO18221.1. Different termination.
L19867 Genomic DNA. Translation: AAA51413.1.
PIRiPC2280.
RefSeqiNP_776490.2. NM_174065.2.
UniGeneiBt.21127.

Genome annotation databases

EnsembliENSBTAT00000017997; ENSBTAP00000017997; ENSBTAG00000013534.
GeneIDi281189.
KEGGibta:281189.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08255 mRNA. Translation: CAA69422.1.
BC119892 mRNA. Translation: AAI19893.1.
AY174179 Genomic DNA. Translation: AAO18221.1. Different termination.
L19867 Genomic DNA. Translation: AAA51413.1.
PIRiPC2280.
RefSeqiNP_776490.2. NM_174065.2.
UniGeneiBt.21127.

3D structure databases

ProteinModelPortaliQ28115.
SMRiQ28115. Positions 63-100, 290-368.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ28115. 2 interactions.
STRINGi9913.ENSBTAP00000017997.

PTM databases

iPTMnetiQ28115.

Proteomic databases

PaxDbiQ28115.
PRIDEiQ28115.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000017997; ENSBTAP00000017997; ENSBTAG00000013534.
GeneIDi281189.
KEGGibta:281189.

Organism-specific databases

CTDi2670.

Phylogenomic databases

eggNOGiENOG410IJXF. Eukaryota.
ENOG410Y9QE. LUCA.
GeneTreeiENSGT00830000128228.
HOGENOMiHOG000230977.
HOVERGENiHBG013015.
InParanoidiQ28115.
KOiK05640.
OMAiHLQEYQE.
OrthoDBiEOG7FV3Q8.
TreeFamiTF330122.

Enzyme and pathway databases

ReactomeiR-BTA-1251985. Nuclear signaling by ERBB4.

Miscellaneous databases

NextBioi20805246.

Family and domain databases

InterProiIPR027701. GFAP.
IPR001664. IF.
IPR006821. Intermed_filament_DNA-bd.
IPR018039. Intermediate_filament_CS.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PTHR23239:SF41. PTHR23239:SF41. 1 hit.
PfamiPF00038. Filament. 1 hit.
PF04732. Filament_head. 1 hit.
[Graphical view]
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Use of a heterologous monoclonal antibody for cloning and detection of glial fibrillary acidic protein in the bovine ventricular ependyma."
    Bouchard P., Ravet V., Meiniel R., Creveaux I., Meiniel A., Vellet A., Vigues B.
    Cell Tissue Res. 298:207-216(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Subcommissural organ.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Thalamus.
  3. "Nucleotide sequence of the bovine glial fibrillary acidic protein (GFAP) gene."
    Guertler M., Alter T., Froeb A., Lange B., Johne R., Luecker E., Fehlhaber K.
    Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-416.
  4. "Conformation polymorphisms and targeted marker development."
    Kirkpatrick B.W., Hart G.L.
    Anim. Genet. 25:77-82(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 197-227.

Entry informationi

Entry nameiGFAP_BOVIN
AccessioniPrimary (citable) accession number: Q28115
Secondary accession number(s): Q0P5L4, Q866S9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 3, 2007
Last modified: May 11, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.