Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glial fibrillary acidic protein

Gene

GFAP

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-BTA-1251985. Nuclear signaling by ERBB4.

Names & Taxonomyi

Protein namesi
Recommended name:
Glial fibrillary acidic protein
Short name:
GFAP
Gene namesi
Name:GFAP
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 19

Subcellular locationi

  • Cytoplasm By similarity

  • Note: Associated with intermediate filaments.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Intermediate filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000638041 – 428Glial fibrillary acidic proteinAdd BLAST428

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7Phosphothreonine; by AURKB and ROCK1By similarity1
Modified residuei12Omega-N-methylarginineBy similarity1
Modified residuei13Phosphoserine; by AURKB and ROCK1By similarity1
Modified residuei26CitrullineBy similarity1
Modified residuei32CitrullineBy similarity1
Modified residuei34Phosphoserine; by AURKB and ROCK1By similarity1
Modified residuei78PhosphoserineBy similarity1
Modified residuei106PhosphothreonineBy similarity1
Modified residuei146PhosphothreonineBy similarity1
Modified residuei266CitrullineBy similarity1
Modified residuei319PhosphoserineBy similarity1
Modified residuei379PhosphothreonineBy similarity1
Modified residuei381PhosphoserineBy similarity1
Modified residuei402CitrullineBy similarity1
Modified residuei412CitrullineBy similarity1

Post-translational modificationi

Phosphorylated by PKN1.By similarity

Keywords - PTMi

Citrullination, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ28115.
PeptideAtlasiQ28115.
PRIDEiQ28115.

PTM databases

iPTMnetiQ28115.

Expressioni

Gene expression databases

BgeeiENSBTAG00000013534.

Interactioni

Subunit structurei

Interacts with SYNM.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
PASKQ96RG22EBI-907866,EBI-1042651From a different organism.

Protein-protein interaction databases

IntActiQ28115. 2 interactors.
STRINGi9913.ENSBTAP00000017997.

Structurei

3D structure databases

ProteinModelPortaliQ28115.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 68HeadAdd BLAST68
Regioni69 – 373RodAdd BLAST305
Regioni69 – 100Coil 1AAdd BLAST32
Regioni101 – 111Linker 1Add BLAST11
Regioni112 – 210Coil 1BAdd BLAST99
Regioni211 – 226Linker 12Add BLAST16
Regioni227 – 248Coil 2AAdd BLAST22
Regioni249 – 252Linker 24
Regioni253 – 373Coil 2BAdd BLAST121
Regioni374 – 428TailAdd BLAST55

Sequence similaritiesi

Belongs to the intermediate filament family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IJXF. Eukaryota.
ENOG410Y9QE. LUCA.
GeneTreeiENSGT00830000128228.
HOGENOMiHOG000230977.
HOVERGENiHBG013015.
InParanoidiQ28115.
KOiK05640.
OMAiHLQEYQE.
OrthoDBiEOG091G12MK.
TreeFamiTF330122.

Family and domain databases

InterProiIPR027701. GFAP.
IPR001664. IF.
IPR006821. Intermed_filament_DNA-bd.
IPR018039. Intermediate_filament_CS.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PTHR23239:SF41. PTHR23239:SF41. 1 hit.
PfamiPF00038. Filament. 1 hit.
PF04732. Filament_head. 1 hit.
[Graphical view]
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q28115-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERRRVTSAT RRSYVSSSEM VVGGRRLGPG TRLSLARMPP PLPARVDFSL
60 70 80 90 100
AGALNSGFKE TRASERAEMM ELNDRFASYI EKVRFLEQQN KALAAELNQL
110 120 130 140 150
RAKEPTKLAD VYQAELRELR LRLDQLTANS ARLEVERDNL AQDLGTLRQK
160 170 180 190 200
LQDETNQRLE AENNLAAYRQ EADEATLARL DLERKIESLE EEIRFLRKIH
210 220 230 240 250
EEEVRELQEQ LAQQQVHVEM DVAKPDLTAA LREIRTQYEA VASSNMHEAE
260 270 280 290 300
EWYRSKFADL NDAAARNAEL LRQAKHEAND YRRQLQALTC DLESLRGTNE
310 320 330 340 350
SLERQMREQE ERHAREAASY QEALARLEEE GQSLKDEMAR HLQEYQDLLN
360 370 380 390 400
VKLALDIEIA TYRKLLEGEE NRITIPVQTF SNLQIRETSL DTKSVSEGHL
410 420
KRNIVVKTVE MRDGEVIKES KQEHKDVM
Length:428
Mass (Da):49,512
Last modified:April 3, 2007 - v2
Checksum:i8DCCDF3E848BAD05
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti265A → R in CAA69422 (PubMed:10571109).Curated1
Sequence conflicti265A → R in AAO18221 (Ref. 3) Curated1
Sequence conflicti271L → V in CAA69422 (PubMed:10571109).Curated1
Sequence conflicti271L → V in AAO18221 (Ref. 3) Curated1
Sequence conflicti311 – 312ER → DA in CAA69422 (PubMed:10571109).Curated2
Sequence conflicti311 – 312ER → DA in AAO18221 (Ref. 3) Curated2
Sequence conflicti372R → Q in AAO18221 (Ref. 3) Curated1
Sequence conflicti421K → P in CAA69422 (PubMed:10571109).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08255 mRNA. Translation: CAA69422.1.
BC119892 mRNA. Translation: AAI19893.1.
AY174179 Genomic DNA. Translation: AAO18221.1. Different termination.
L19867 Genomic DNA. Translation: AAA51413.1.
PIRiPC2280.
RefSeqiNP_776490.2. NM_174065.2.
UniGeneiBt.21127.

Genome annotation databases

EnsembliENSBTAT00000017997; ENSBTAP00000017997; ENSBTAG00000013534.
GeneIDi281189.
KEGGibta:281189.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08255 mRNA. Translation: CAA69422.1.
BC119892 mRNA. Translation: AAI19893.1.
AY174179 Genomic DNA. Translation: AAO18221.1. Different termination.
L19867 Genomic DNA. Translation: AAA51413.1.
PIRiPC2280.
RefSeqiNP_776490.2. NM_174065.2.
UniGeneiBt.21127.

3D structure databases

ProteinModelPortaliQ28115.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ28115. 2 interactors.
STRINGi9913.ENSBTAP00000017997.

PTM databases

iPTMnetiQ28115.

Proteomic databases

PaxDbiQ28115.
PeptideAtlasiQ28115.
PRIDEiQ28115.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000017997; ENSBTAP00000017997; ENSBTAG00000013534.
GeneIDi281189.
KEGGibta:281189.

Organism-specific databases

CTDi2670.

Phylogenomic databases

eggNOGiENOG410IJXF. Eukaryota.
ENOG410Y9QE. LUCA.
GeneTreeiENSGT00830000128228.
HOGENOMiHOG000230977.
HOVERGENiHBG013015.
InParanoidiQ28115.
KOiK05640.
OMAiHLQEYQE.
OrthoDBiEOG091G12MK.
TreeFamiTF330122.

Enzyme and pathway databases

ReactomeiR-BTA-1251985. Nuclear signaling by ERBB4.

Gene expression databases

BgeeiENSBTAG00000013534.

Family and domain databases

InterProiIPR027701. GFAP.
IPR001664. IF.
IPR006821. Intermed_filament_DNA-bd.
IPR018039. Intermediate_filament_CS.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PTHR23239:SF41. PTHR23239:SF41. 1 hit.
PfamiPF00038. Filament. 1 hit.
PF04732. Filament_head. 1 hit.
[Graphical view]
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGFAP_BOVIN
AccessioniPrimary (citable) accession number: Q28115
Secondary accession number(s): Q0P5L4, Q866S9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 3, 2007
Last modified: November 2, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.