Q28067 (KCMB1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calcium-activated potassium channel subunit beta-1

Alternative name(s):
BK channel subunit beta-1
Short name=BKbeta
Short name=BKbeta1
Calcium-activated potassium channel, subfamily M subunit beta-1
Short name=Calcium-activated potassium channel subunit beta
Charybdotoxin receptor subunit beta-1
K(VCA)beta-1
Maxi K channel subunit beta-1
Slo-beta-1
Short name=Slo-beta

Gene names

Name:

KCNMB1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	191 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Increases the apparent Ca2+/voltage sensitivity of the KCNMA1 channel. It also modifies KCNMA1 channel kinetics and alters its pharmacological properties. It slows down the activation and the deactivation kinetics of the channel. Acts as a negative regulator of smooth muscle contraction by enhancing the calcium sensitivity to KCNMA1. Its presence is also a requirement for internal binding of the KCNMA1 channel opener dehydrosoyasaponin I (DHS-1) triterpene glycoside and for external binding of the agonist hormone 17-beta-estradiol (E2). Increases the binding activity of charybdotoxin (CTX) toxin to KCNMA1 peptide blocker by increasing the CTX association rate and decreasing the dissociation rate By similarity.
Subunit structure	Interacts with KCNMA1 tetramer. There are probably 4 molecules of KCMNB1 per KCNMA1 tetramer By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Post-translational modification	N-glycosylated By similarity.
Sequence similarities	Belongs to the KCNMB (TC 8.A.14.1) family. KCNMB1 subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Ionic channel
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 191	190	Calcium-activated potassium channel subunit beta-1		PRO_0000187044
Regions
	Topological domain	2 – 18	17	Cytoplasmic Potential
	Transmembrane	19 – 39	21	Helical; Name=1; Potential
	Topological domain	40 – 157	118	Extracellular Potential
	Transmembrane	158 – 178	21	Helical; Name=2; Potential
	Topological domain	179 – 191	13	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	80	1	N-linked (GlcNAc...) Potential
	Glycosylation	142	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q28067 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 111C15E4A2B6B545	FASTA	191	21,957
	10 20 30 40 50 60 MGKKLVMAQR RGETRALCLG VAMVVGAVIT YYILGTTVLP LYQKSVWTQE STCHLIETNI 70 80 90 100 110 120 RDQEELEGKR VPQYPCLWVN VSSVGRWAVL YHTEDTRDQN HQCSYIPSSL DNYQVARADV 130 140 150 160 170 180 EKVRARFHEN QDFFCFSTTR ENETSVLYRR LYGPQSLLFS LFWPTFLLTG GLLIIVMVKI 190 NQSLSILAAQ R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary sequence and immunological characterization of beta-subunit of high conductance Ca(2+)-activated K+ channel from smooth muscle." Knaus H.-G., Folander K., Garcia-Calvo M., Garcia M.L., Kaczorowski G.J., Smith M., Swanson R. J. Biol. Chem. 269:17274-17278(1994) [PubMed: 8006036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-29. Tissue: Aorta and Trachea.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Uterus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L26101 mRNA. Translation: AAA21741.1. BC147983 mRNA. Translation: AAI47984.1.
IPI	IPI00697173.
PIR	A54165.
RefSeq	NP_001001141.1. NM_001001141.1.
UniGene	Bt.62471.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q28067.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000003886; ENSBTAP00000003886; ENSBTAG00000002985. ENSBTAT00000056280; ENSBTAP00000049066; ENSBTAG00000002985.
GeneID	407176.
KEGG	bta:407176.
Organism-specific databases
CTD	3779.
Phylogenomic databases
eggNOG	maNOG08126.
GeneTree	ENSGT00390000015997.
HOVERGEN	HBG052223.
InParanoid	Q28067.
OMA	YQRLYGP.
OrthoDB	EOG47SSFT.
PhylomeDB	Q28067.
Family and domain databases
InterPro	IPR003930. K_chnl_Ca-activ_BK_bsu. [Graphical view]
KO	K04937.
PANTHER	PTHR10258. BK_channel_beta. 1 hit.
Pfam	PF03185. CaKB. 1 hit. [Graphical view]
PRINTS	PR01450. BKCHANNELB.
ProtoNet	Search...

Entry information

Entry name

KCMB1_BOVIN

Accession

Primary (citable) accession number: Q28067
Secondary accession number(s): A6QLJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 74 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program