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Protein

Adseverin

Gene

SCIN

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ca2+-dependent actin filament-severing protein that has a regulatory function in exocytosis by affecting the organization of the microfilament network underneath the plasma membrane. In vitro, also has barbed end capping and nucleating activities in the presence of Ca2+ (PubMed:1651929, PubMed:1847925, PubMed:8780652). Severing activity is inhibited by phosphatidylinositol 4,5-bis-phosphate (PIP2) (PubMed:8780652). Required for megakaryocyte differentiation, maturation, polyploidization and apoptosis with the release of platelet-like particles (By similarity). Plays a role in osteoclastogenesis (OCG) and actin cytoskeletal organization in osteoclasts (By similarity). Regulates chondrocyte proliferation and differentiation (By similarity). Inhibits cell proliferation and tumorigenesis. Signaling is mediated by MAPK, p38 and JNK pathways (By similarity).By similarity3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi538Calcium 1By similarity1
Metal bindingi539Calcium 1By similarity1
Metal bindingi562Calcium 1By similarity1
Metal bindingi643Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi644Calcium 2By similarity1
Metal bindingi666Calcium 2By similarity1

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • actin filament binding Source: UniProtKB
  • calcium ion binding Source: UniProtKB
  • phosphatidylinositol-4,5-bisphosphate binding Source: UniProtKB
  • phosphatidylserine binding Source: UniProtKB

GO - Biological processi

  • actin filament capping Source: UniProtKB-KW
  • actin filament severing Source: UniProtKB
  • actin nucleation Source: UniProtKB
  • calcium ion regulated exocytosis Source: UniProtKB
  • negative regulation of cell proliferation Source: UniProtKB
  • positive regulation of apoptotic process Source: UniProtKB
  • positive regulation of megakaryocyte differentiation Source: UniProtKB
  • positive regulation of secretion Source: UniProtKB
  • regulation of chondrocyte differentiation Source: UniProtKB

Keywordsi

Molecular functionActin capping, Actin-binding
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Adseverin
Alternative name(s):
Scinderin
Short name:
SC
Gene namesi
Name:SCIN
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  • cell cortex Source: UniProtKB
  • cell junction Source: UniProtKB-KW
  • cell projection Source: UniProtKB-KW
  • cytoplasm Source: UniProtKB
  • podosome Source: UniProtKB-SubCell

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002187431 – 715AdseverinAdd BLAST715

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei102PhosphotyrosineBy similarity1
Modified residuei599PhosphotyrosineBy similarity1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PeptideAtlasiQ28046.
PRIDEiQ28046.

Expressioni

Tissue specificityi

In the adrenal gland, expressed in the medulla but, in the cortex, found only in diffuse parts.

Interactioni

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • actin filament binding Source: UniProtKB

Structurei

3D structure databases

ProteinModelPortaliQ28046.
SMRiQ28046.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati27 – 77Gelsolin-like 1Add BLAST51
Repeati148 – 188Gelsolin-like 2Add BLAST41
Repeati265 – 307Gelsolin-like 3Add BLAST43
Repeati398 – 451Gelsolin-like 4Add BLAST54
Repeati523 – 564Gelsolin-like 5Add BLAST42
Repeati626 – 668Gelsolin-like 6Add BLAST43

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 363Actin-severingSequence analysisAdd BLAST363
Regioni112 – 119Polyphosphoinositide binding8
Regioni138 – 146Polyphosphoinositide binding9
Regioni364 – 715Actin-binding, Ca-sensitiveSequence analysisAdd BLAST352
Regioni364 – 715Ca(2+)-dependent actin bindingBy similarityAdd BLAST352

Sequence similaritiesi

Belongs to the villin/gelsolin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000233630.
HOVERGENiHBG004183.
InParanoidiQ28046.
KOiK05768.

Family and domain databases

Gene3Di3.40.20.10. 6 hits.
InterProiView protein in InterPro
IPR029006. ADF-H/Gelsolin-like_dom.
IPR030012. Adseverin.
IPR007123. Gelsolin-like_dom.
IPR007122. Villin/Gelsolin.
PANTHERiPTHR11977. PTHR11977. 1 hit.
PTHR11977:SF68. PTHR11977:SF68. 1 hit.
PfamiView protein in Pfam
PF00626. Gelsolin. 6 hits.
PRINTSiPR00597. GELSOLIN.
SMARTiView protein in SMART
SM00262. GEL. 6 hits.
SUPFAMiSSF82754. SSF82754. 1 hit.

Sequencei

Sequence statusi: Complete.

Q28046-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQGLYHEEF ARAGKRAGLQ VWRIEKLELV PVPESAYGNF YVGDAYLVLH
60 70 80 90 100
TTQASRGFTY RLHFWLGKEC TQDESTAAAI FTVQMDDYLG GKPVQNRELQ
110 120 130 140 150
GYESTDFVGY FKGGLKYKAG GVASGLNHVL TNDLTAQRLL HVKGRRVVRA
160 170 180 190 200
TEVPLSWDSF NKGDCFIIDL GTEIYQWCGS SCNKYERLKA SQVAIGIRDN
210 220 230 240 250
ERKGRAQLIV VEEGSEPSEL TKVLGEKPKL RDGEDDDDIK ADITNRKMAK
260 270 280 290 300
LYMVSDASGS MKVSLVAEEN PFSMAMLLSE ECFILDHGAA KQIFVWKGKD
310 320 330 340 350
ANPQERKAAM KTAEEFLQQM NYSTNTQIQV LPEGGETPIF KQFFKDWRDR
360 370 380 390 400
DQSDGFGKVY VTEKVAHVKQ IPFDASKLHS SPQMAAQHHV VDDGSGKVQI
410 420 430 440 450
WRVENNGRVE IDRNSYGEFY GGDCYIILYT YPRGQIIYTW QGANATRDEL
460 470 480 490 500
TTSAFLTVQL DRSLGGQAVQ IRVSQGKEPA HLLSLFKDKP LIIYKNGTSK
510 520 530 540 550
KEGQAPAPPI RLFQVRRNLA SITRIMEVDV DANSLNSNDV FVLKLRQNNG
560 570 580 590 600
YIWIGKGSTQ EEEKGAEYVA SVLKCKTSTI QEGKEPEEFW NSLGGKKDYQ
610 620 630 640 650
TSPLLESQAE DHPPRLYGCS NKTGRFIIEE VPGEFTQDDL AEDDVMLLDA
660 670 680 690 700
WEQIFIWIGK DANEVEKSES LKSAKIYLET DPSGRDKRTP IVIIKQGHEP
710
PTFTGWFLGW DSSRW
Length:715
Mass (Da):80,578
Last modified:November 1, 1997 - v1
Checksum:iA1FD7DDFC02DA044
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti231 – 232RD → TH in CAA55227 (Ref. 2) Curated2
Sequence conflicti330V → L in CAA55227 (Ref. 2) Curated1
Sequence conflicti454 – 466AFLTV…RSLGG → DSRLFSWIDPSGD in CAA55227 (Ref. 2) CuratedAdd BLAST13
Sequence conflicti520 – 522ASI → DSY in CAA55227 (Ref. 2) Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26549 mRNA. Translation: BAA05548.1.
X78479 mRNA. Translation: CAA55227.1.
PIRiA53209.
I45832.
RefSeqiNP_776602.1. NM_174177.2.
UniGeneiBt.3980.

Genome annotation databases

GeneIDi281478.
KEGGibta:281478.

Similar proteinsi

Entry informationi

Entry nameiADSV_BOVIN
AccessioniPrimary (citable) accession number: Q28046
Secondary accession number(s): Q27948
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 15, 2017
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families