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Protein

Sodium/hydrogen exchanger 1

Gene

SLC9A1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei161Channel pore-liningBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Sodium transport, Transport

Keywords - Ligandi

Calmodulin-binding, Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/hydrogen exchanger 1
Alternative name(s):
Na(+)/H(+) exchanger 1
Short name:
NHE-1
Solute carrier family 9 member 1
Gene namesi
Name:SLC9A1
Synonyms:NHE1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11CytoplasmicSequence analysisAdd BLAST11
Transmembranei12 – 32Helical; Name=M1Sequence analysisAdd BLAST21
Topological domaini33 – 104ExtracellularSequence analysisAdd BLAST72
Transmembranei105 – 127Helical; Name=M2Sequence analysisAdd BLAST23
Topological domaini128 – 129CytoplasmicSequence analysis2
Transmembranei130 – 149Helical; Name=M3Sequence analysisAdd BLAST20
Topological domaini150 – 154ExtracellularSequence analysis5
Transmembranei155 – 174Helical; Name=M4Sequence analysisAdd BLAST20
Topological domaini175 – 191CytoplasmicSequence analysisAdd BLAST17
Transmembranei192 – 211Helical; Name=M5Sequence analysisAdd BLAST20
Topological domaini212 – 227ExtracellularSequence analysisAdd BLAST16
Transmembranei228 – 247Helical; Name=M6Sequence analysisAdd BLAST20
Topological domaini248 – 256CytoplasmicSequence analysis9
Transmembranei257 – 276Helical; Name=M7Sequence analysisAdd BLAST20
Topological domaini277 – 294ExtracellularSequence analysisAdd BLAST18
Transmembranei295 – 315Helical; Name=M8Sequence analysisAdd BLAST21
Topological domaini316 – 338CytoplasmicSequence analysisAdd BLAST23
Transmembranei339 – 358Helical; Name=M9Sequence analysisAdd BLAST20
Topological domaini359 – 381ExtracellularSequence analysisAdd BLAST23
Intramembranei382 – 402Name=H10By similarityAdd BLAST21
Topological domaini403 – 410ExtracellularSequence analysis8
Transmembranei411 – 430Helical; Name=M10Sequence analysisAdd BLAST20
Topological domaini431 – 448CytoplasmicSequence analysisAdd BLAST18
Transmembranei449 – 470Helical; Name=M11Sequence analysisAdd BLAST22
Topological domaini471 – 479ExtracellularSequence analysis9
Transmembranei480 – 499Helical; Name=M12Sequence analysisAdd BLAST20
Topological domaini500 – 817CytoplasmicSequence analysisAdd BLAST318

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000523451 – 817Sodium/hydrogen exchanger 1Add BLAST817

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi75N-linked (GlcNAc...)Sequence analysis1
Glycosylationi370N-linked (GlcNAc...)Sequence analysis1
Modified residuei599PhosphoserineBy similarity1
Modified residuei602PhosphoserineBy similarity1
Modified residuei603PhosphothreonineBy similarity1
Modified residuei605PhosphoserineBy similarity1
Modified residuei693PhosphoserineBy similarity1
Modified residuei697PhosphoserineBy similarity1
Modified residuei703PhosphoserineBy similarity1
Modified residuei723PhosphoserineBy similarity1
Modified residuei726PhosphoserineBy similarity1
Modified residuei729PhosphoserineBy similarity1
Modified residuei787PhosphoserineBy similarity1
Modified residuei789PhosphoserineBy similarity1
Modified residuei798PhosphoserineBy similarity1

Post-translational modificationi

O-glycosylated.By similarity
Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is reduced by CHP1 (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ28036.
PRIDEiQ28036.

Interactioni

Subunit structurei

Oligomer. Interacts with calmodulin and TESC. Interacts (via the juxtamembrane region of the cytoplasmic C-terminal domain) with CHP1; the interaction occurs at the plasma membrane in a calcium-dependent manner. Interacts with CHP2; the interaction occurs in a calcium-dependent manner (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000048292.

Structurei

3D structure databases

ProteinModelPortaliQ28036.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni516 – 539Interaction with CHP2By similarityAdd BLAST24

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1966. Eukaryota.
COG0025. LUCA.
HOGENOMiHOG000247044.
HOVERGENiHBG052615.
InParanoidiQ28036.
KOiK05742.

Family and domain databases

InterProiIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR001970. Na/H_exchanger_1.
IPR004709. NaH_exchanger.
IPR032103. NHE_CaM-bd.
[Graphical view]
PANTHERiPTHR10110. PTHR10110. 1 hit.
PTHR10110:SF59. PTHR10110:SF59. 1 hit.
PfamiPF00999. Na_H_Exchanger. 1 hit.
PF16644. NEXCaM_BD. 1 hit.
[Graphical view]
PRINTSiPR01084. NAHEXCHNGR.
PR01085. NAHEXCHNGR1.
TIGRFAMsiTIGR00840. b_cpa1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q28036-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLWPGASGL PPPRIFPSLL VVVALVGLLP VLRSYGLQIS PTASTIRGSE
60 70 80 90 100
PPRERSIGDV TTAPPELAPE SRPVNHSFAE HSPKARKAFP VLGIDYQHVR
110 120 130 140 150
IPFEIALWIL LACLMKIGFH VIPTISSIVP ESCLLIVVGL LVGGLIKGVG
160 170 180 190 200
ETPPILQSEV FFLFLLPPII LDAGYFLPLR QFTENLGTIL IFAVVGTLWN
210 220 230 240 250
AFFLGGLMYA VCLVGGEQIN NIGLLENLLF GSIISAVDPV AVLAVFEEIH
260 270 280 290 300
INELLHILVF GESLLNDAVT VVLYHLFEEF ANYDRVGIVD IILGFLSFFV
310 320 330 340 350
VSLGGVFVGV VYGVIAAFTS RFTSHIRVIE PLFVFLYSYM AYLSAELFHL
360 370 380 390 400
SGIMALIASG VVMRPYVEAN ISHKSHTTIK YFLKMWSSVS ETLIFIFLGV
410 420 430 440 450
STVAGSHHWN WTFVISTLLF CLIARVLGVL GLTWFINKFR IVKLTPKDQF
460 470 480 490 500
IIAYGGLRGA IAFSLGYLLD KKHFPMCDLF LTAIITVIFF TVFVQGMTIR
510 520 530 540 550
PLVDLLAVKK KQETKRSINE EIHTQFLDHL LTGIEDICGH YGHHHWKDKL
560 570 580 590 600
NRFNKKYVKK CLIAGERSKE PQLIAFYHKM EMKQAIELVE SGGMGKIPSA
610 620 630 640 650
VSTVSMQNIH PKSLPAERIL PALSKDKEEE IRKILRNNLQ KTRQRLRSYN
660 670 680 690 700
RHTLVADPYE EAWNQMLLRR QKARQLEQKI SNYLTVPAHK LDSPTMSRAR
710 720 730 740 750
IGSDPLAYEP KADLPVITID PASPQSPESV DLVNEELKGK VLGLSRDPGR
760 770 780 790 800
LAEEEEDDNG PRWLTMRTKE PSSPGTDDVF TPAPSDSPSS QRIQRCLSDP
810
GPHPEPGEGE PFIPKGQ
Length:817
Mass (Da):91,017
Last modified:November 1, 1997 - v1
Checksum:i6617E99D3B012920
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49432 mRNA. Translation: AAA91483.1.
RefSeqiNP_777258.1. NM_174833.2.
UniGeneiBt.528.

Genome annotation databases

GeneIDi317654.
KEGGibta:317654.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49432 mRNA. Translation: AAA91483.1.
RefSeqiNP_777258.1. NM_174833.2.
UniGeneiBt.528.

3D structure databases

ProteinModelPortaliQ28036.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000048292.

Proteomic databases

PaxDbiQ28036.
PRIDEiQ28036.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi317654.
KEGGibta:317654.

Organism-specific databases

CTDi6548.

Phylogenomic databases

eggNOGiKOG1966. Eukaryota.
COG0025. LUCA.
HOGENOMiHOG000247044.
HOVERGENiHBG052615.
InParanoidiQ28036.
KOiK05742.

Family and domain databases

InterProiIPR006153. Cation/H_exchanger.
IPR018422. Cation/H_exchanger_CPA1.
IPR001970. Na/H_exchanger_1.
IPR004709. NaH_exchanger.
IPR032103. NHE_CaM-bd.
[Graphical view]
PANTHERiPTHR10110. PTHR10110. 1 hit.
PTHR10110:SF59. PTHR10110:SF59. 1 hit.
PfamiPF00999. Na_H_Exchanger. 1 hit.
PF16644. NEXCaM_BD. 1 hit.
[Graphical view]
PRINTSiPR01084. NAHEXCHNGR.
PR01085. NAHEXCHNGR1.
TIGRFAMsiTIGR00840. b_cpa1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSL9A1_BOVIN
AccessioniPrimary (citable) accession number: Q28036
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 5, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

The region between transmembrane regions M4 and M5 and between M6 and M7 (also termed intracellular loops IL2 and IL4, respectively) seem to be localized at least in part in the membrane. The hydrophobic region H10 is proposed to be located within the membrane.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.