Reviewed, UniProtKB/Swiss-Prot Q28035 (GSTA1_BOVIN)

Names and origin

Protein names

Recommended name:
    Glutathione S-transferase A1
    EC=2.5.1.18
Alternative name(s):
    Glutathione S-transferase alpha-1
    GST class-alpha member 1

Gene names

Name:

GSTA1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Expressed in corpus luteum, adrenal gland, testis, liver, lung, thyroid and kidney.
Sequence similarities	Belongs to the GST superfamily. Alpha family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Transferase
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 222	221	Glutathione S-transferase A1		PRO_0000185780
Regions
	Domain	3 – 83	81	GST N-terminal
	Domain	85 – 208	124	GST C-terminal
Experimental info
	Sequence conflict	190 – 191	2	NI → SL in AAB72239. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q28035-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: F2708C849CB04655	FASTA	222	25,452
	10 20 30 40 50 60 MAGKPTLHYF NGRGRMECIR WLLAAAGVEF EEKFIEKPED LDKLKNDGSL MFQQVPMVEI 70 80 90 100 110 120 DGMKLVQTRA ILNYIATKYN LYGKDMKERA LIDMYSEGVA DLGEMIMHFP LCPPAEKDAK 130 140 150 160 170 180 LTLIREKTTN RYLPAFENVL KSHGQDYLVG NKLSRADIHL VELLYYVEEL DPSLLANFPL 190 200 210 220 LKALKARVSN IPAVKKFLQP GSQRKPPTDE KKIEEARKVF KF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"High expression of bovine alpha glutathione S-transferase (GSTA1, GSTA2) subunits is mainly associated with steroidogenically active cells and regulated by gonadotropins in bovine ovarian follicles." Rabahi F., Brule S., Sirois J., Beckers J.-F.M.P., Silversides D.W., Lussier J.G. Endocrinology 140:3507-3517(1999) [PubMed: 10433206] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Holstein. Tissue: Corpus luteum.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U49179 mRNA. Translation: AAB72239.1. BC102540 mRNA. Translation: AAI02541.1.
IPI	IPI00840271.
RefSeq	NP_001071617.1.
UniGene	Bt.227
3D structure databases
HSSP	HSSP built from PDB template 1K3O based on UniProtKB P08263.
SMR	Q28035. Positions 2-222.
ModBase	Search...
Protein-protein interaction databases
STRING	Q28035.
Genome annotation databases
Ensembl	ENSBTAT00000005618; ENSBTAP00000005618; ENSBTAG00000021516; Bos taurus. [Genome view]
GeneID	777644.
KEGG	bta:777644.
Phylogenomic databases
HOVERGEN	Q28035.
Enzyme and pathway databases
BRENDA	2.5.1.18. 251.
Family and domain databases
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR003080. GST_alpha. IPR004046. GST_C. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHER	PTHR11571:SF4. GST_alpha. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PRINTS	PR01266. GSTRNSFRASEA.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTA1_BOVIN

Accession

Primary (citable) accession number: Q28035
Secondary accession number(s): Q3T072

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	September 1, 2009
This is version 67 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)