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Protein

Glucosidase 2 subunit beta

Gene

PRKCSH

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Regulatory subunit of glucosidase II.1 Publication

Pathway:iN-glycan metabolism

This protein is involved in the pathway N-glycan metabolism, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway N-glycan metabolism and in Glycan metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi212 – 235241PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_290037. Calnexin/calreticulin cycle.
REACT_324505. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.
REACT_350372. Advanced glycosylation endproduct receptor signaling.
UniPathwayiUPA00957.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosidase 2 subunit beta
Alternative name(s):
80K-H protein
Glucosidase II subunit beta
Protein kinase C substrate 60.1 kDa protein heavy chain
Short name:
PKCSH
Vacuolar system-associated protein 60
Short name:
VASAP-60
Gene namesi
Name:PRKCSH
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 7

Subcellular locationi

  • Endoplasmic reticulum PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1313By similarityAdd
BLAST
Chaini14 – 533520Glucosidase 2 subunit betaPRO_0000004142Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi71 – 711N-linked (GlcNAc...)Sequence Analysis
Modified residuei88 – 881Phosphoserine; by PKCSequence Analysis
Modified residuei165 – 1651N6-succinyllysineBy similarity
Modified residuei388 – 3881Phosphoserine; by PKCSequence Analysis
Modified residuei395 – 3951Phosphoserine; by PKCSequence Analysis
Modified residuei439 – 4391Phosphoserine; by PKCSequence Analysis
Glycosylationi481 – 4811N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ28034.
PRIDEiQ28034.

Expressioni

Tissue specificityi

Ubiquitous. Highly expressed in liver, spleen, lung, duodenum, stomach, adrenal gland, pituitary, testis, corpus luteum, uterus and fetal ovary.1 Publication

Interactioni

Subunit structurei

Heterodimer of a catalytic alpha subunit (GANAB) and a beta subunit (PRKCSH). Binds glycosylated PTPRC (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000010787.

Structurei

3D structure databases

ProteinModelPortaliQ28034.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini208 – 24336EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini244 – 27936EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini418 – 47356PRKCSHAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi530 – 5334Prevents secretion from ERPROSITE-ProRule annotation

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi299 – 402104Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 1 PRKCSH domain.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG289998.
GeneTreeiENSGT00510000047770.
HOGENOMiHOG000007805.
HOVERGENiHBG051738.
InParanoidiQ28034.
KOiK08288.
OMAiEEKCVYR.
OrthoDBiEOG7DZ8JX.
TreeFamiTF329550.

Family and domain databases

Gene3Di2.70.130.10. 1 hit.
4.10.400.10. 2 hits.
InterProiIPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR026874. Glucosidase_2_bsu.
IPR002172. LDrepeatLR_classA_rpt.
IPR009011. Man6P_isomerase_rcpt-bd_dom.
IPR028146. PRKCSH_N.
[Graphical view]
PANTHERiPTHR12630:SF1. PTHR12630:SF1. 1 hit.
PfamiPF13202. EF-hand_5. 1 hit.
PF12999. PRKCSH-like. 1 hit.
[Graphical view]
SMARTiSM00192. LDLa. 2 hits.
[Graphical view]
SUPFAMiSSF50911. SSF50911. 1 hit.
SSF57424. SSF57424. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q28034-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLLLLLLPM CWAVEVRRPR GVSLTNHHFY DESKPFTCLD GSASIPFDQV
60 70 80 90 100
NDDYCDCKDG SDEPGTAACP NGSFHCTNTG YKALYISSRW VNDGVCDCCD
110 120 130 140 150
GTDEYNSGIV CENTCKEKGR KERETLQQMA EVTREGFRLK KILIEDWKKA
160 170 180 190 200
REEKQKKLIE LQAGKKSLED QVEVLRTLKE EAEKPEEAAK DQHRRLWEEQ
210 220 230 240 250
QAISKEQRER ELAASAFQEL DDDMDGAVSV AELQTHPELD TDGDGALSEG
260 270 280 290 300
EAQTLLGGDA QMDAAFFYDR VWAAIRDKYR SEVLPTEYPP SPPAPDVMEP
310 320 330 340 350
KEEQPPMPSP PTEEEDEDEE DEETEEDEDE EDEDSQGEQP KDAPPPAPAP
360 370 380 390 400
QTASPTEEDR MPPYDEQTQA FINAAQEARN KFEEAERSLK DMEESIRNLE
410 420 430 440 450
QEISFDFGPN GEFAYLYSQC YELTTNEYVY RLCPFKLVSQ KPKLGGSPTS
460 470 480 490 500
LGTWGSWAGP DHDKFSAMKY EQGTGCWQGP NRSTTVRLLC GKETVVTSTT
510 520 530
EPSRCEYLME LMTPAACPEP PPEYPVEGDH DEL
Length:533
Mass (Da):60,151
Last modified:November 1, 1997 - v1
Checksum:i50785B677FB81E01
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49178 mRNA. Translation: AAA92060.1.
AF299077 Genomic DNA. Translation: AAQ14482.1.
BT030688 mRNA. Translation: ABS45004.1.
BC104524 mRNA. Translation: AAI04525.1.
RefSeqiNP_788835.1. NM_176662.1.
XP_005208774.1. XM_005208717.1.
UniGeneiBt.6139.

Genome annotation databases

EnsembliENSBTAT00000010787; ENSBTAP00000010787; ENSBTAG00000008202.
GeneIDi338067.
KEGGibta:338067.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49178 mRNA. Translation: AAA92060.1.
AF299077 Genomic DNA. Translation: AAQ14482.1.
BT030688 mRNA. Translation: ABS45004.1.
BC104524 mRNA. Translation: AAI04525.1.
RefSeqiNP_788835.1. NM_176662.1.
XP_005208774.1. XM_005208717.1.
UniGeneiBt.6139.

3D structure databases

ProteinModelPortaliQ28034.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000010787.

Proteomic databases

PaxDbiQ28034.
PRIDEiQ28034.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000010787; ENSBTAP00000010787; ENSBTAG00000008202.
GeneIDi338067.
KEGGibta:338067.

Organism-specific databases

CTDi5589.

Phylogenomic databases

eggNOGiNOG289998.
GeneTreeiENSGT00510000047770.
HOGENOMiHOG000007805.
HOVERGENiHBG051738.
InParanoidiQ28034.
KOiK08288.
OMAiEEKCVYR.
OrthoDBiEOG7DZ8JX.
TreeFamiTF329550.

Enzyme and pathway databases

UniPathwayiUPA00957.
ReactomeiREACT_290037. Calnexin/calreticulin cycle.
REACT_324505. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.
REACT_350372. Advanced glycosylation endproduct receptor signaling.

Miscellaneous databases

NextBioi20812518.

Family and domain databases

Gene3Di2.70.130.10. 1 hit.
4.10.400.10. 2 hits.
InterProiIPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR026874. Glucosidase_2_bsu.
IPR002172. LDrepeatLR_classA_rpt.
IPR009011. Man6P_isomerase_rcpt-bd_dom.
IPR028146. PRKCSH_N.
[Graphical view]
PANTHERiPTHR12630:SF1. PTHR12630:SF1. 1 hit.
PfamiPF13202. EF-hand_5. 1 hit.
PF12999. PRKCSH-like. 1 hit.
[Graphical view]
SMARTiSM00192. LDLa. 2 hits.
[Graphical view]
SUPFAMiSSF50911. SSF50911. 1 hit.
SSF57424. SSF57424. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Vacuolar system-associated protein-60: a protein characterized from bovine granulosa and luteal cells that is associated with intracellular vesicles and related to human 80K-H and murine beta-glucosidase II."
    Brule S., Rabahi F., Faure R., Beckers J.-F.M.P., Silversides D.W., Lussier J.G.
    Biol. Reprod. 62:642-654(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Strain: Holstein.
    Tissue: Corpus luteum.
  2. "Bos taurus vacuolar system asociated protein-60 (VASAP-60) promotor and complete gene sequences."
    Brule S., Silversides D., Lussier J.G.
    Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Ascending colon.

Entry informationi

Entry nameiGLU2B_BOVIN
AccessioniPrimary (citable) accession number: Q28034
Secondary accession number(s): A7E3R7, Q3SX37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 22, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.