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Protein

Glucosidase 2 subunit beta

Gene

PRKCSH

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Regulatory subunit of glucosidase II.1 Publication

Pathwayi: N-glycan metabolism

This protein is involved in the pathway N-glycan metabolism, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway N-glycan metabolism and in Glycan metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi212 – 2351PROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-BTA-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-BTA-532668 N-glycan trimming in the ER and Calnexin/Calreticulin cycle
R-BTA-8957275 Post-translational protein phosphorylation
R-BTA-901042 Calnexin/calreticulin cycle
UniPathwayiUPA00957

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosidase 2 subunit beta
Alternative name(s):
80K-H protein
Glucosidase II subunit beta
Protein kinase C substrate 60.1 kDa protein heavy chain
Short name:
PKCSH
Vacuolar system-associated protein 60
Short name:
VASAP-60
Gene namesi
Name:PRKCSH
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 7

Organism-specific databases

VGNCiVGNC:33335 PRKCSH

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 13By similarityAdd BLAST13
ChainiPRO_000000414214 – 533Glucosidase 2 subunit betaAdd BLAST520

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei23PhosphoserineBy similarity1
Glycosylationi71N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei88Phosphoserine; by PKCSequence analysis1
Modified residuei165N6-succinyllysineBy similarity1
Modified residuei167PhosphoserineBy similarity1
Modified residuei388Phosphoserine; by PKCSequence analysis1
Modified residuei395Phosphoserine; by PKCSequence analysis1
Modified residuei439Phosphoserine; by PKCSequence analysis1
Glycosylationi481N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ28034
PeptideAtlasiQ28034
PRIDEiQ28034

Expressioni

Tissue specificityi

Ubiquitous. Highly expressed in liver, spleen, lung, duodenum, stomach, adrenal gland, pituitary, testis, corpus luteum, uterus and fetal ovary.1 Publication

Gene expression databases

BgeeiENSBTAG00000008202

Interactioni

Subunit structurei

Heterodimer of a catalytic alpha subunit (GANAB) and a beta subunit (PRKCSH). Binds glycosylated PTPRC (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000010787

Structurei

3D structure databases

ProteinModelPortaliQ28034
SMRiQ28034
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini208 – 243EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini244 – 279EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini418 – 473PRKCSHAdd BLAST56

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi530 – 533Prevents secretion from ERPROSITE-ProRule annotation4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi299 – 402Asp/Glu-rich (acidic)Add BLAST104

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG2397 Eukaryota
ENOG410XPE3 LUCA
GeneTreeiENSGT00510000047770
HOGENOMiHOG000007805
HOVERGENiHBG051738
InParanoidiQ28034
KOiK08288
OMAiCYELTTN
OrthoDBiEOG091G06YU
TreeFamiTF329550

Family and domain databases

CDDicd00112 LDLa, 1 hit
Gene3Di2.70.130.10, 1 hit
InterProiView protein in InterPro
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR026874 Glucosidase_2_bsu
IPR036055 LDL_receptor-like_sf
IPR002172 LDrepeatLR_classA_rpt
IPR009011 Man6P_isomerase_rcpt-bd_dom_sf
IPR036607 PRKCSH
IPR028146 PRKCSH_N
PANTHERiPTHR12630:SF1 PTHR12630:SF1, 1 hit
PfamiView protein in Pfam
PF13202 EF-hand_5, 1 hit
PF12999 PRKCSH-like, 1 hit
PF13015 PRKCSH_1, 1 hit
SUPFAMiSSF57424 SSF57424, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 1 hit
PS50222 EF_HAND_2, 1 hit
PS00014 ER_TARGET, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q28034-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLLLLLLPM CWAVEVRRPR GVSLTNHHFY DESKPFTCLD GSASIPFDQV
60 70 80 90 100
NDDYCDCKDG SDEPGTAACP NGSFHCTNTG YKALYISSRW VNDGVCDCCD
110 120 130 140 150
GTDEYNSGIV CENTCKEKGR KERETLQQMA EVTREGFRLK KILIEDWKKA
160 170 180 190 200
REEKQKKLIE LQAGKKSLED QVEVLRTLKE EAEKPEEAAK DQHRRLWEEQ
210 220 230 240 250
QAISKEQRER ELAASAFQEL DDDMDGAVSV AELQTHPELD TDGDGALSEG
260 270 280 290 300
EAQTLLGGDA QMDAAFFYDR VWAAIRDKYR SEVLPTEYPP SPPAPDVMEP
310 320 330 340 350
KEEQPPMPSP PTEEEDEDEE DEETEEDEDE EDEDSQGEQP KDAPPPAPAP
360 370 380 390 400
QTASPTEEDR MPPYDEQTQA FINAAQEARN KFEEAERSLK DMEESIRNLE
410 420 430 440 450
QEISFDFGPN GEFAYLYSQC YELTTNEYVY RLCPFKLVSQ KPKLGGSPTS
460 470 480 490 500
LGTWGSWAGP DHDKFSAMKY EQGTGCWQGP NRSTTVRLLC GKETVVTSTT
510 520 530
EPSRCEYLME LMTPAACPEP PPEYPVEGDH DEL
Length:533
Mass (Da):60,151
Last modified:November 1, 1997 - v1
Checksum:i50785B677FB81E01
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49178 mRNA Translation: AAA92060.1
AF299077 Genomic DNA Translation: AAQ14482.1
BT030688 mRNA Translation: ABS45004.1
BC104524 mRNA Translation: AAI04525.1
RefSeqiNP_788835.1, NM_176662.1
XP_005208774.1, XM_005208717.1
UniGeneiBt.6139

Genome annotation databases

EnsembliENSBTAT00000010787; ENSBTAP00000010787; ENSBTAG00000008202
GeneIDi338067
KEGGibta:338067

Similar proteinsi

Entry informationi

Entry nameiGLU2B_BOVIN
AccessioniPrimary (citable) accession number: Q28034
Secondary accession number(s): A7E3R7, Q3SX37
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health