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Q28034 (GLU2B_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glucosidase 2 subunit beta
Alternative name(s):
80K-H protein
Glucosidase II subunit beta
Protein kinase C substrate 60.1 kDa protein heavy chain
Short name=PKCSH
Vacuolar system-associated protein 60
Short name=VASAP-60
Gene names
Name:PRKCSH
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length533 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Regulatory subunit of glucosidase II. Ref.1

Pathway

Glycan metabolism; N-glycan metabolism.

Subunit structure

Heterodimer of a catalytic alpha subunit (GANAB) and a beta subunit (PRKCSH). Binds glycosylated PTPRC By similarity.

Subcellular location

Endoplasmic reticulum By similarity.

Tissue specificity

Ubiquitous. Highly expressed in liver, spleen, lung, duodenum, stomach, adrenal gland, pituitary, testis, corpus luteum, uterus and fetal ovary. Ref.1

Sequence similarities

Contains 2 EF-hand domains.

Contains 1 PRKCSH domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1313 By similarity
Chain14 – 533520Glucosidase 2 subunit beta
PRO_0000004142

Regions

Domain208 – 24336EF-hand 1
Domain244 – 27936EF-hand 2
Domain418 – 47356PRKCSH
Calcium binding212 – 235241 Potential
Motif530 – 5334Prevents secretion from ER Potential
Compositional bias299 – 402104Asp/Glu-rich (acidic)

Amino acid modifications

Modified residue881Phosphoserine; by PKC Potential
Modified residue3881Phosphoserine; by PKC Potential
Modified residue3951Phosphoserine; by PKC Potential
Modified residue4391Phosphoserine; by PKC Potential
Glycosylation711N-linked (GlcNAc...) Potential
Glycosylation4811N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q28034 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 50785B677FB81E01

FASTA53360,151
        10         20         30         40         50         60 
MLLLLLLLPM CWAVEVRRPR GVSLTNHHFY DESKPFTCLD GSASIPFDQV NDDYCDCKDG 

        70         80         90        100        110        120 
SDEPGTAACP NGSFHCTNTG YKALYISSRW VNDGVCDCCD GTDEYNSGIV CENTCKEKGR 

       130        140        150        160        170        180 
KERETLQQMA EVTREGFRLK KILIEDWKKA REEKQKKLIE LQAGKKSLED QVEVLRTLKE 

       190        200        210        220        230        240 
EAEKPEEAAK DQHRRLWEEQ QAISKEQRER ELAASAFQEL DDDMDGAVSV AELQTHPELD 

       250        260        270        280        290        300 
TDGDGALSEG EAQTLLGGDA QMDAAFFYDR VWAAIRDKYR SEVLPTEYPP SPPAPDVMEP 

       310        320        330        340        350        360 
KEEQPPMPSP PTEEEDEDEE DEETEEDEDE EDEDSQGEQP KDAPPPAPAP QTASPTEEDR 

       370        380        390        400        410        420 
MPPYDEQTQA FINAAQEARN KFEEAERSLK DMEESIRNLE QEISFDFGPN GEFAYLYSQC 

       430        440        450        460        470        480 
YELTTNEYVY RLCPFKLVSQ KPKLGGSPTS LGTWGSWAGP DHDKFSAMKY EQGTGCWQGP 

       490        500        510        520        530 
NRSTTVRLLC GKETVVTSTT EPSRCEYLME LMTPAACPEP PPEYPVEGDH DEL

« Hide

References

« Hide 'large scale' references
[1]"Vacuolar system-associated protein-60: a protein characterized from bovine granulosa and luteal cells that is associated with intracellular vesicles and related to human 80K-H and murine beta-glucosidase II."
Brule S., Rabahi F., Faure R., Beckers J.-F.M.P., Silversides D.W., Lussier J.G.
Biol. Reprod. 62:642-654(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
Strain: Holstein.
Tissue: Corpus luteum.
[2]"Bos taurus vacuolar system asociated protein-60 (VASAP-60) promotor and complete gene sequences."
Brule S., Silversides D., Lussier J.G.
Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Characterization of 954 bovine full-CDS cDNA sequences."
Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.
BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]NIH - Mammalian Gene Collection (MGC) project
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Ascending colon.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U49178 mRNA. Translation: AAA92060.1.
AF299077 Genomic DNA. Translation: AAQ14482.1.
BT030688 mRNA. Translation: ABS45004.1.
BC104524 mRNA. Translation: AAI04525.1.
IPIIPI00692278.
RefSeqNP_788835.1. NM_176662.1.
UniGeneBt.6139.

3D structure databases

ProteinModelPortalQ28034.
ModBaseSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000010787.

Proteomic databases

PaxDbQ28034.
PRIDEQ28034.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000010787; ENSBTAP00000010787; ENSBTAG00000008202.
GeneID338067.
KEGGbta:338067.

Organism-specific databases

CTD5589.

Phylogenomic databases

eggNOGNOG289998.
GeneTreeENSGT00510000047770.
HOGENOMHOG000007805.
HOVERGENHBG051738.
InParanoidQ28034.
KOK08288.
OMACTNAGYK.
OrthoDBEOG4DFPNG.

Enzyme and pathway databases

BioCycCATTLE:338067-MONOMER.
UniPathwayUPA00957.

Family and domain databases

Gene3D4.10.400.10. 2 hits.
InterProIPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR026874. Glucosidase_2_bsu.
IPR002172. LDrepeatLR_classA_rpt.
IPR009011. Man6P_isomerase_rcpt-bd_dom.
[Graphical view]
PANTHERPTHR12630:SF1. PTHR12630:SF1. 1 hit.
PfamPF13202. EF_hand_3. 1 hit.
[Graphical view]
SMARTSM00192. LDLa. 2 hits.
[Graphical view]
SUPFAMSSF57424. LDL_rcpt_classA_cys-rich. 1 hit.
SSF50911. Man6php_recept. 1 hit.
PROSITEPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20812518.

Entry information

Entry nameGLU2B_BOVIN
AccessionPrimary (citable) accession number: Q28034
Secondary accession number(s): A7E3R7, Q3SX37
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 29, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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