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Q28034

- GLU2B_BOVIN

UniProt

Q28034 - GLU2B_BOVIN

Protein

Glucosidase 2 subunit beta

Gene

PRKCSH

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Regulatory subunit of glucosidase II.1 Publication

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi212 – 235241PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. hydrolase activity Source: UniProtKB-KW
    3. RNA binding Source: Ensembl

    GO - Biological processi

    1. in utero embryonic development Source: Ensembl
    2. liver development Source: Ensembl
    3. negative regulation of neuron projection development Source: Ensembl
    4. N-glycan processing Source: InterPro
    5. nitrogen compound metabolic process Source: Ensembl
    6. renal system development Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_204725. Advanced glycosylation endproduct receptor signaling.
    REACT_208919. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.
    REACT_215263. Calnexin/calreticulin cycle.
    UniPathwayiUPA00957.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucosidase 2 subunit beta
    Alternative name(s):
    80K-H protein
    Glucosidase II subunit beta
    Protein kinase C substrate 60.1 kDa protein heavy chain
    Short name:
    PKCSH
    Vacuolar system-associated protein 60
    Short name:
    VASAP-60
    Gene namesi
    Name:PRKCSH
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 7

    Subcellular locationi

    Endoplasmic reticulum PROSITE-ProRule annotation

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1313By similarityAdd
    BLAST
    Chaini14 – 533520Glucosidase 2 subunit betaPRO_0000004142Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi71 – 711N-linked (GlcNAc...)Sequence Analysis
    Modified residuei88 – 881Phosphoserine; by PKCSequence Analysis
    Modified residuei165 – 1651N6-succinyllysineBy similarity
    Modified residuei388 – 3881Phosphoserine; by PKCSequence Analysis
    Modified residuei395 – 3951Phosphoserine; by PKCSequence Analysis
    Modified residuei439 – 4391Phosphoserine; by PKCSequence Analysis
    Glycosylationi481 – 4811N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ28034.
    PRIDEiQ28034.

    Expressioni

    Tissue specificityi

    Ubiquitous. Highly expressed in liver, spleen, lung, duodenum, stomach, adrenal gland, pituitary, testis, corpus luteum, uterus and fetal ovary.1 Publication

    Interactioni

    Subunit structurei

    Heterodimer of a catalytic alpha subunit (GANAB) and a beta subunit (PRKCSH). Binds glycosylated PTPRC By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000010787.

    Structurei

    3D structure databases

    ProteinModelPortaliQ28034.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini208 – 24336EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini244 – 27936EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini418 – 47356PRKCSHAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi530 – 5334Prevents secretion from ERPROSITE-ProRule annotation

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi299 – 402104Asp/Glu-rich (acidic)Add
    BLAST

    Sequence similaritiesi

    Contains 2 EF-hand domains.PROSITE-ProRule annotation
    Contains 1 PRKCSH domain.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG289998.
    GeneTreeiENSGT00510000047770.
    HOGENOMiHOG000007805.
    HOVERGENiHBG051738.
    InParanoidiQ28034.
    KOiK08288.
    OMAiGQHCWNG.
    OrthoDBiEOG7DZ8JX.
    TreeFamiTF329550.

    Family and domain databases

    Gene3Di2.70.130.10. 1 hit.
    4.10.400.10. 2 hits.
    InterProiIPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR026874. Glucosidase_2_bsu.
    IPR002172. LDrepeatLR_classA_rpt.
    IPR009011. Man6P_isomerase_rcpt-bd_dom.
    IPR028146. PRKCSH_N.
    [Graphical view]
    PANTHERiPTHR12630:SF1. PTHR12630:SF1. 1 hit.
    PfamiPF13202. EF-hand_5. 1 hit.
    PF12999. PRKCSH-like. 1 hit.
    [Graphical view]
    SMARTiSM00192. LDLa. 2 hits.
    [Graphical view]
    SUPFAMiSSF50911. SSF50911. 1 hit.
    SSF57424. SSF57424. 1 hit.
    PROSITEiPS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS00014. ER_TARGET. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q28034-1 [UniParc]FASTAAdd to Basket

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    MLLLLLLLPM CWAVEVRRPR GVSLTNHHFY DESKPFTCLD GSASIPFDQV    50
    NDDYCDCKDG SDEPGTAACP NGSFHCTNTG YKALYISSRW VNDGVCDCCD 100
    GTDEYNSGIV CENTCKEKGR KERETLQQMA EVTREGFRLK KILIEDWKKA 150
    REEKQKKLIE LQAGKKSLED QVEVLRTLKE EAEKPEEAAK DQHRRLWEEQ 200
    QAISKEQRER ELAASAFQEL DDDMDGAVSV AELQTHPELD TDGDGALSEG 250
    EAQTLLGGDA QMDAAFFYDR VWAAIRDKYR SEVLPTEYPP SPPAPDVMEP 300
    KEEQPPMPSP PTEEEDEDEE DEETEEDEDE EDEDSQGEQP KDAPPPAPAP 350
    QTASPTEEDR MPPYDEQTQA FINAAQEARN KFEEAERSLK DMEESIRNLE 400
    QEISFDFGPN GEFAYLYSQC YELTTNEYVY RLCPFKLVSQ KPKLGGSPTS 450
    LGTWGSWAGP DHDKFSAMKY EQGTGCWQGP NRSTTVRLLC GKETVVTSTT 500
    EPSRCEYLME LMTPAACPEP PPEYPVEGDH DEL 533
    Length:533
    Mass (Da):60,151
    Last modified:November 1, 1997 - v1
    Checksum:i50785B677FB81E01
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U49178 mRNA. Translation: AAA92060.1.
    AF299077 Genomic DNA. Translation: AAQ14482.1.
    BT030688 mRNA. Translation: ABS45004.1.
    BC104524 mRNA. Translation: AAI04525.1.
    RefSeqiNP_788835.1. NM_176662.1.
    XP_005208774.1. XM_005208717.1.
    UniGeneiBt.6139.

    Genome annotation databases

    EnsembliENSBTAT00000010787; ENSBTAP00000010787; ENSBTAG00000008202.
    GeneIDi338067.
    KEGGibta:338067.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U49178 mRNA. Translation: AAA92060.1 .
    AF299077 Genomic DNA. Translation: AAQ14482.1 .
    BT030688 mRNA. Translation: ABS45004.1 .
    BC104524 mRNA. Translation: AAI04525.1 .
    RefSeqi NP_788835.1. NM_176662.1.
    XP_005208774.1. XM_005208717.1.
    UniGenei Bt.6139.

    3D structure databases

    ProteinModelPortali Q28034.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000010787.

    Proteomic databases

    PaxDbi Q28034.
    PRIDEi Q28034.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000010787 ; ENSBTAP00000010787 ; ENSBTAG00000008202 .
    GeneIDi 338067.
    KEGGi bta:338067.

    Organism-specific databases

    CTDi 5589.

    Phylogenomic databases

    eggNOGi NOG289998.
    GeneTreei ENSGT00510000047770.
    HOGENOMi HOG000007805.
    HOVERGENi HBG051738.
    InParanoidi Q28034.
    KOi K08288.
    OMAi GQHCWNG.
    OrthoDBi EOG7DZ8JX.
    TreeFami TF329550.

    Enzyme and pathway databases

    UniPathwayi UPA00957 .
    Reactomei REACT_204725. Advanced glycosylation endproduct receptor signaling.
    REACT_208919. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.
    REACT_215263. Calnexin/calreticulin cycle.

    Miscellaneous databases

    NextBioi 20812518.

    Family and domain databases

    Gene3Di 2.70.130.10. 1 hit.
    4.10.400.10. 2 hits.
    InterProi IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR026874. Glucosidase_2_bsu.
    IPR002172. LDrepeatLR_classA_rpt.
    IPR009011. Man6P_isomerase_rcpt-bd_dom.
    IPR028146. PRKCSH_N.
    [Graphical view ]
    PANTHERi PTHR12630:SF1. PTHR12630:SF1. 1 hit.
    Pfami PF13202. EF-hand_5. 1 hit.
    PF12999. PRKCSH-like. 1 hit.
    [Graphical view ]
    SMARTi SM00192. LDLa. 2 hits.
    [Graphical view ]
    SUPFAMi SSF50911. SSF50911. 1 hit.
    SSF57424. SSF57424. 1 hit.
    PROSITEi PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS00014. ER_TARGET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Vacuolar system-associated protein-60: a protein characterized from bovine granulosa and luteal cells that is associated with intracellular vesicles and related to human 80K-H and murine beta-glucosidase II."
      Brule S., Rabahi F., Faure R., Beckers J.-F.M.P., Silversides D.W., Lussier J.G.
      Biol. Reprod. 62:642-654(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
      Strain: Holstein.
      Tissue: Corpus luteum.
    2. "Bos taurus vacuolar system asociated protein-60 (VASAP-60) promotor and complete gene sequences."
      Brule S., Silversides D., Lussier J.G.
      Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. NIH - Mammalian Gene Collection (MGC) project
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Ascending colon.

    Entry informationi

    Entry nameiGLU2B_BOVIN
    AccessioniPrimary (citable) accession number: Q28034
    Secondary accession number(s): A7E3R7, Q3SX37
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3