Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q28017

- PAFA_BOVIN

UniProt

Q28017 - PAFA_BOVIN

Protein

Platelet-activating factor acetylhydrolase

Gene

PLA2G7

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.

    Catalytic activityi

    1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei274 – 2741NucleophileBy similarity
    Active sitei297 – 2971Charge relay systemPROSITE-ProRule annotation
    Active sitei352 – 3521Charge relay systemPROSITE-ProRule annotation

    GO - Molecular functioni

    1. 1-alkyl-2-acetylglycerophosphocholine esterase activity Source: UniProtKB-EC

    GO - Biological processi

    1. lipid catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Platelet-activating factor acetylhydrolase (EC:3.1.1.47)
    Short name:
    PAF acetylhydrolase
    Alternative name(s):
    1-alkyl-2-acetylglycerophosphocholine esterase
    2-acetyl-1-alkylglycerophosphocholine esterase
    LDL-associated phospholipase A2
    Short name:
    LDL-PLA(2)
    PAF 2-acylhydrolase
    Gene namesi
    Name:PLA2G7
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121By similarityAdd
    BLAST
    Chaini22 – 444423Platelet-activating factor acetylhydrolasePRO_0000017830Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi60 – 601N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi424 – 4241N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi434 – 4341N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ28017.

    Expressioni

    Tissue specificityi

    Plasma.

    Interactioni

    Structurei

    3D structure databases

    ProteinModelPortaliQ28017.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AB hydrolase superfamily. Lipase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG4188.
    HOGENOMiHOG000008053.
    HOVERGENiHBG001322.
    InParanoidiQ28017.

    Family and domain databases

    Gene3Di3.40.50.1820. 3 hits.
    InterProiIPR029058. AB_hydrolase.
    IPR005065. PAF_acetylhydro.
    IPR016715. PAF_acetylhydro_eukaryote.
    [Graphical view]
    PANTHERiPTHR10272. PTHR10272. 1 hit.
    PfamiPF03403. PAF-AH_p_II. 1 hit.
    [Graphical view]
    PIRSFiPIRSF018169. PAF_acetylhydrolase. 1 hit.
    SUPFAMiSSF53474. SSF53474. 3 hits.
    PROSITEiPS00120. LIPASE_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q28017-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLPSKLHALF CLCTCLALVY PFDWQDLNPV AYIESPAWVS KIQALMAAAN    50
    IGQSKIPRGN GSYSVGCTDL MFDYTNKGTF LRLYYPSQDD DHSDTLWIPN 100
    KEYFLGLSKF LGTHWLVGKI MGLFFGSMTT PAAWNAHLRT GEKYPLIIFS 150
    HGLGAFRTIY SAIGIDLASH GFIVAAVEHR DGSASSTYYF KDQSAVEIGN 200
    KSWLYLRTLK RGEEEFPLRN EQLRQRAKEC SQALSLILDI DHGRPVTNVL 250
    DLEFDVEQLK DSIDRDKIAI IGHSFGGATV IQTLSEDQRF RCGIALDAWM 300
    FPVGDEVYSR IPQPLFFINS ERFQYPSNII RMKKCFLPDR ERKMITIRGS 350
    VHQNFVDFTF ATSKIIGYLF TLKGDIDSNV AISLSNKASL AFLQKHLGLQ 400
    KDFDQWDSLV EGEDHNLIPG TNINTTNHQA ILQNSTGIER PNLD 444
    Length:444
    Mass (Da):50,133
    Last modified:November 1, 1997 - v1
    Checksum:i97689917BE2F4C38
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U34247 mRNA. Translation: AAC48483.1.
    UniGeneiBt.42359.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U34247 mRNA. Translation: AAC48483.1 .
    UniGenei Bt.42359.

    3D structure databases

    ProteinModelPortali Q28017.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi Q28017.
    ChEMBLi CHEMBL2441.

    Proteomic databases

    PRIDEi Q28017.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG4188.
    HOGENOMi HOG000008053.
    HOVERGENi HBG001322.
    InParanoidi Q28017.

    Family and domain databases

    Gene3Di 3.40.50.1820. 3 hits.
    InterProi IPR029058. AB_hydrolase.
    IPR005065. PAF_acetylhydro.
    IPR016715. PAF_acetylhydro_eukaryote.
    [Graphical view ]
    PANTHERi PTHR10272. PTHR10272. 1 hit.
    Pfami PF03403. PAF-AH_p_II. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF018169. PAF_acetylhydrolase. 1 hit.
    SUPFAMi SSF53474. SSF53474. 3 hits.
    PROSITEi PS00120. LIPASE_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Plasma platelet-activating factor acetylhydrolase is a secreted phospholipase A2 with a catalytic triad."
      Tjoelker L.W., Eberhardt C., Unger J., le Trong H., Zimmerman G.A., McIntyre T.M., Stafforini D.M., Prescott S.M., Gray P.W.
      J. Biol. Chem. 270:25481-25487(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Spleen.

    Entry informationi

    Entry nameiPAFA_BOVIN
    AccessioniPrimary (citable) accession number: Q28017
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3