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Q28017 (PAFA_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Platelet-activating factor acetylhydrolase

Short name=PAF acetylhydrolase
EC=3.1.1.47
Alternative name(s):
1-alkyl-2-acetylglycerophosphocholine esterase
2-acetyl-1-alkylglycerophosphocholine esterase
LDL-associated phospholipase A2
Short name=LDL-PLA(2)
PAF 2-acylhydrolase
Gene names
Name:PLA2G7
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length444 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.

Catalytic activity

1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

Subcellular location

Secretedextracellular space.

Tissue specificity

Plasma.

Sequence similarities

Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
   Biological processLipid degradation
Lipid metabolism
   Cellular componentSecreted
   DomainSignal
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processlipid catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function1-alkyl-2-acetylglycerophosphocholine esterase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 By similarity
Chain22 – 444423Platelet-activating factor acetylhydrolase
PRO_0000017830

Sites

Active site2741Nucleophile By similarity
Active site2971Charge relay system By similarity
Active site3521Charge relay system By similarity

Amino acid modifications

Glycosylation601N-linked (GlcNAc...) Potential
Glycosylation2001N-linked (GlcNAc...) Potential
Glycosylation4241N-linked (GlcNAc...) Potential
Glycosylation4341N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q28017 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 97689917BE2F4C38

FASTA44450,133
        10         20         30         40         50         60 
MLPSKLHALF CLCTCLALVY PFDWQDLNPV AYIESPAWVS KIQALMAAAN IGQSKIPRGN 

        70         80         90        100        110        120 
GSYSVGCTDL MFDYTNKGTF LRLYYPSQDD DHSDTLWIPN KEYFLGLSKF LGTHWLVGKI 

       130        140        150        160        170        180 
MGLFFGSMTT PAAWNAHLRT GEKYPLIIFS HGLGAFRTIY SAIGIDLASH GFIVAAVEHR 

       190        200        210        220        230        240 
DGSASSTYYF KDQSAVEIGN KSWLYLRTLK RGEEEFPLRN EQLRQRAKEC SQALSLILDI 

       250        260        270        280        290        300 
DHGRPVTNVL DLEFDVEQLK DSIDRDKIAI IGHSFGGATV IQTLSEDQRF RCGIALDAWM 

       310        320        330        340        350        360 
FPVGDEVYSR IPQPLFFINS ERFQYPSNII RMKKCFLPDR ERKMITIRGS VHQNFVDFTF 

       370        380        390        400        410        420 
ATSKIIGYLF TLKGDIDSNV AISLSNKASL AFLQKHLGLQ KDFDQWDSLV EGEDHNLIPG 

       430        440 
TNINTTNHQA ILQNSTGIER PNLD 

« Hide

References

[1]"Plasma platelet-activating factor acetylhydrolase is a secreted phospholipase A2 with a catalytic triad."
Tjoelker L.W., Eberhardt C., Unger J., le Trong H., Zimmerman G.A., McIntyre T.M., Stafforini D.M., Prescott S.M., Gray P.W.
J. Biol. Chem. 270:25481-25487(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Spleen.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U34247 mRNA. Translation: AAC48483.1.
UniGeneBt.42359.

3D structure databases

ProteinModelPortalQ28017.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBQ28017.
ChEMBLCHEMBL2441.

Proteomic databases

PRIDEQ28017.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG4188.
HOGENOMHOG000008053.
HOVERGENHBG001322.
InParanoidQ28017.

Family and domain databases

InterProIPR005065. PAF_acetylhydro.
IPR016715. PAF_acetylhydro_eukaryote.
[Graphical view]
PANTHERPTHR10272. PTHR10272. 1 hit.
PfamPF03403. PAF-AH_p_II. 1 hit.
[Graphical view]
PIRSFPIRSF018169. PAF_acetylhydrolase. 1 hit.
PROSITEPS00120. LIPASE_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePAFA_BOVIN
AccessionPrimary (citable) accession number: Q28017
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: January 22, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families