Q28013 (RASA3_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras GTPase-activating protein 3

Alternative name(s):
GAP1(IP4BP)
Ins P4-binding protein

Gene names

Name:

RASA3

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	834 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Inhibitory regulator of the Ras-cyclic AMP pathway. May bind inositol tetrakisphosphate (IP4).
Sequence similarities	Contains 1 Btk-type zinc finger. Contains 2 C2 domains. Contains 1 PH domain. Contains 1 Ras-GAP domain.

Ontologies

Keywords
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro negative regulation of Ras protein signal transduction Inferred from sequence or structural similarity. Source: RefGenome
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: RefGenome intrinsic to internal side of plasma membrane Inferred from sequence or structural similarity. Source: RefGenome
Molecular_function	Ras GTPase activator activity Inferred from sequence or structural similarity. Source: RefGenome metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 834	834	Ras GTPase-activating protein 3		PRO_0000056641
Regions
	Domain	1 – 96	96	C2 1
	Domain	132 – 247	116	C2 2
	Domain	330 – 524	195	Ras-GAP
	Domain	576 – 677	102	PH
	Zinc finger	679 – 715	37	Btk-type
Amino acid modifications
	Modified residue	66	1	Phosphotyrosine By similarity
	Modified residue	110	1	Phosphothreonine By similarity
	Modified residue	809	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q28013 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: AB996368A27174BA	FASTA	834	95,386
	10 20 30 40 50 60 MAVEEEGLRV FQSVKIKIGE AKNLPTYPGP NKMRDCYCTV NLDQEEVFRT KVVEKSLCPF 70 80 90 100 110 120 YGEDFYCEIP RSFRHLSFYI FDRDVFRRDS IIGKVAIKKE DLQKYHNRDT WFQLQHVDAD 130 140 150 160 170 180 SEVQGKVHLE LRLSEVITDS GVVCHKLATR ILECQGLPIV NGQCDPYATV TLAGPCRSEA 190 200 210 220 230 240 KKTKVKKKTN NPQFDEVFYF EVTRPCSYSR KSHFDFEDED VDKLEIRVDL WNASNLKFGD 250 260 270 280 290 300 EFLGELRVPL KVLRQSSPHE AWYFLQPRDN GSKSLKPGDL GSLRLNVVYT EDHVFSSDYY 310 320 330 340 350 360 SPLRDLLLKS ADVEPVSASA AHILGEVCRE KQEAAIPLVR LFLHYGRVVP FISAIASAEV 370 380 390 400 410 420 RRTQDPNTIF RGNSLTSKCI DETMKLAGMQ YLHVTLKPTI EEICQSHKSC EIDPVRLKDG 430 440 450 460 470 480 ESLESNMENL RQFVDRVFSV ITKSGVSCPT VMCDIFFSLR EAAAKRFQDD LDVRYTAVSS 490 500 510 520 530 540 FIFLRFFAPA ILSPNLFQLT PHHTDPQTSR TLTLVSKTIQ TLGSLSKSKS ASFKESYMAA 550 560 570 580 590 600 FYEFFNEQKY ADAVKNFLDL ISSSGRRDPK SVQQPILLKE GFMIKRAQGR KRFGMKNFKK 610 620 630 640 650 660 RWFRLTNHEF TYQKSKGDPP LYSIPIENIL AVEPLEEESF KMKNMFQVIQ PERALYIQAN 670 680 690 700 710 720 NCVEAKAWID ILTKVSQCNQ KRLAVYHPSA YLNGHWLCCR ASSDTAAGCS PCTGGLPANI 730 740 750 760 770 780 QLDIDGDRET ERIYSLSSSY MSKLETMQEA CGSRSVYDGP EQEEYSTFII DDPQETYKTL 790 800 810 820 830 KQVVAGVGAL EQEHAQYKRD KFRRTKYGSQ EHPIGDKSFQ SYIRQQSETP AHSM

References

[1]	"A novel GTPase-activating protein for R-Ras." Yamamoto T., Matsui T., Nakafuku M., Iwamatsu A., Kaibuchi K. J. Biol. Chem. 270:30557-30561(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U30857 mRNA. Translation: AAC48500.1.
IPI	IPI01028353.
UniGene	Bt.5371.
3D structure databases
ProteinModelPortal	Q28013.
ModBase	Search...
Proteomic databases
PRIDE	Q28013.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	NOG323905.
HOGENOM	HOG000007183.
HOVERGEN	HBG055643.
InParanoid	Q28013.
OrthoDB	EOG4D7Z50.
Family and domain databases
Gene3D	1.10.506.10. 1 hit. 2.30.29.30. 1 hit.
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR018029. C2_membr_targeting. IPR011993. PH_like_dom. IPR001849. Pleckstrin_homology. IPR001936. RasGAP. IPR023152. RasGAP_CS. IPR008936. Rho_GTPase_activation_prot. IPR001562. Znf_Btk_motif. [Graphical view]
Pfam	PF00779. BTK. 1 hit. PF00168. C2. 2 hits. PF00169. PH. 1 hit. PF00616. RasGAP. 1 hit. [Graphical view]
PRINTS	PR00402. TECBTKDOMAIN.
SMART	SM00107. BTK. 1 hit. SM00239. C2. 2 hits. SM00233. PH. 1 hit. SM00323. RasGAP. 1 hit. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 2 hits. SSF48350. Rho_GAP. 1 hit.
PROSITE	PS50004. C2. 2 hits. PS50003. PH_DOMAIN. 1 hit. PS00509. RAS_GTPASE_ACTIV_1. 1 hit. PS50018. RAS_GTPASE_ACTIV_2. 1 hit. PS51113. ZF_BTK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RASA3_BOVIN

Accession

Primary (citable) accession number: Q28013

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 4, 2001
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program