Reviewed, UniProtKB/Swiss-Prot Q27YE5 (L_MOBVC)

Names and origin

Protein names

Recommended name:
    RNA-directed RNA polymerase
    EC=2.7.7.48
Alternative name(s):
    Protein L

Gene names

Name:

L

Organism

Mobala virus (isolate Rat/Central African Republic/Acar 3080/1983) (MOBV)

Taxonomic identifier

55097 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Arenaviridae › Arenavirus › Old world arenaviruses

Virus host

Praomys (African soft-furred rats) [TaxID: 10111]

Protein attributes

Sequence length	2220 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Homomultimerizes; the oligomeric structure is essential for the polymerase activity. Interacts with the nucleocapsid protein N By similarity. Interacts with protein Z; this interaction inhibits viral transcription and replication By similarity.
Subcellular location	Virion. Host cytoplasm.
Sequence similarities	Belongs to the arenaviridae RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication
Cellular component	Host cytoplasm Virion
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Gene Ontology (GO)
Biological process	transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2220	2220	RNA-directed RNA polymerase		PRO_0000361642
Regions
	Domain	1174 – 1370	197	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	Q27YE5-1 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: C2C0DB50EFA05508	FASTA	2,220	255,250
	10 20 30 40 50 60 MEEQISEVKD IISKYLSNDD RLAKQKLAFL VQSEPKLLLI EGLKLLSLCI EIDSCEANGC 70 80 90 100 110 120 DHNTKELSVE NFLSENRVLC PGLPMVVPDG FKLNGNVLMI LECFVRSSPA NFEQKYREDL 130 140 150 160 170 180 VKLNSLKEDL MTVGITMLPL IDGRTNFQTD RLPEWANERF RTLLFSLLAF SQESSRMFEE 190 200 210 220 230 240 AEYSRLCESL NVSGGKRSGI ENINILSDHR SEHFDELLKL CHVGINNHMS SLDVKREIIQ 250 260 270 280 290 300 EFQAFRNKLQ NGVIERQFLR VNREELIKAF NEMYTLRVGD KPELLDSLLN DYYHSCPLIT 310 320 330 340 350 360 MLYCELPNGK SCQSDISHVR GWRSLLNKVK SLRLINTRRK LMLIFDSILL LAHMKDLSVN 370 380 390 400 410 420 GHLVESEWMG SSFLSVNDRL VSLPATQKDL KTWLQRRTNR LSHSHQSQSA YEVFSTMVNR 430 440 450 460 470 480 VLNKAKEVLL LVNLTFKDYN VDEDILSESS FTEMMSLEVN GVEPTINYEK NPIDRFSYNI 490 500 510 520 530 540 QAMDPDNQSD LKRLSSISLA LVNSMKTSST VKLRQNEHGK LRYKCVRCKE AYYQDFLIEG 550 560 570 580 590 600 HRLMLIYQKT GECSKCYSVN DAVVGELCSF YADPKRYFPA IFSDSVLQEM IDTMISWLTE 610 620 630 640 650 660 CSELKEFIKE IKSLLKMVVM VVLTNPTKRI QKFLQNLRYF TMAYVSEYHH KDLLEKLRED 670 680 690 700 710 720 LITNCEFLLY RITRSILNIV FNVNVTTMIT NRFKFILNLS YLCHLITKET PDRLTDQIKC 730 740 750 760 770 780 FEKFIEPKMK FDSVNVNPLE PADQEELRSL LMSADKFLSK PDCFGDEGIL FKTPGVSRKI 790 800 810 820 830 840 FSMMVSSFNN GSLFKQAELK NGVKDPLVVS GCATALDLAS NKSVVVNKYT DGDRIIEYDY 850 860 870 880 890 900 DKLVATAVCQ LSEVFSRKGK YVLSKEDYDF KIQQIMSDLV IGRSKLHGSE IGLNSCEEVD 910 920 930 940 950 960 EVLIEGGAAD YFDSIKQSVD TVMSKFSWSG SESSATLKSE CSIDDLSLAL QDKAQLRLIR 970 980 990 1000 1010 1020 NELSCHMVED FDVMTLPYDT YEEICKSVYS DPSLRSKYFY LESLESCPLT KMAQAVCTRT 1030 1040 1050 1060 1070 1080 FHDEEYFQCF KSLLLQMNAN KLSGKFNHYK SKCLNFKLDR DRLFNETRIS ERESNSEALS 1090 1100 1110 1120 1130 1140 KALSLTNCTT AALKNLCFYS QESPQSYNSQ GPDTGRLKFS LSYKEQVGGN RELYIGDLRT 1150 1160 1170 1180 1190 1200 KMFTRLIEDY FEALTSQFKG SCLNDEHEFE NAVFSMKFNV SLGLLSYSLD HSKWGPMMTP 1210 1220 1230 1240 1250 1260 FLFLATLQNI NWPSLDTLSD AKSRDYVSSM LSWHIHKLVE VPFNVVTAMM KSFIKSKLGL 1270 1280 1290 1300 1310 1320 KKNLSETMTE RFFFEHFRLG KVPSHISSIL DKGQGILQNT SDFYGLISER FINYCISCLY 1330 1340 1350 1360 1370 1380 EGNVDAFTSS DDQISLFDKS LSDLLEKDPD EFEYILEFHN YLSDQLNKFI SPKSVKGNFA 1390 1400 1410 1420 1430 1440 AEFKSRFFVW GDEVPLLTKF VAASLHNIKC KEPHQLAETI DTIIDQAVAN GVPVKLCNIV 1450 1460 1470 1480 1490 1500 QERTLNLLRY AQYPIDPFLM FCSSDVKDWV DGNRGYRIMR NIEMLEPNGT RKVRSFLRRL 1510 1520 1530 1540 1550 1560 YNNLKTGLLH EEFTAAYLSG DPYQSLAKLS KIFDTEILND EELGLSWLNL SAYYPLRMVL 1570 1580 1590 1600 1610 1620 RQKVIYTGAV NVEEEKLPTI VKTLQNKLSS NFTRGAQKLL SEAINRSAFQ SCIASGFVGL 1630 1640 1650 1660 1670 1680 CRTLGSKCVR GPERENFYIK SIMNQSMMME GVSRELVMGV DVWRVRNPLD NSRAQQKWGN 1690 1700 1710 1720 1730 1740 YFRPILWDYL CIALSTALEI GSWVLGEPKL KSPLPQMKFR PCDYFPMKPS VTRLLEDKVG 1750 1760 1770 1780 1790 1800 FNHIIHSFRR LYPDIFEKHL LPFMSDLAST KMKWSPRVKF LDLCVMLDVN CEAMSLVSHI 1810 1820 1830 1840 1850 1860 VKWKREEHYV ILSDELSISH DRSHESLADE RVVSTEDVSE NFLRQIYFES FARPFVATSR 1870 1880 1890 1900 1910 1920 TLGSFTWFPH KTSLPESEGL ASLGPFGTFI EKVIFKGIER PMYRHDLFSG YAWLDFDFGE 1930 1940 1950 1960 1970 1980 FYINSSKLIQ YGLTEMRYFE DLSEFMSMLS SLKPGSIEIS LTVNFQVKSQ GESLREKFFI 1990 2000 2010 2020 2030 2040 HCKFYGSFDV DGKFEFNNIG VQYSGAINRS AVLDCWRLIL TNSHFLGDKV IWHLNTANIK 2050 2060 2070 2080 2090 2100 DYLKDGSMVG EVVPIEVIIN RDALRLDTLD FERVGPDVNV VPLVVKDGYI FEGDKKLVPF 2110 2120 2130 2140 2150 2160 NPSIHDQDFE ILVKELCIDD KELLKDMIQK MITVRGSQGL QWHSLDIVAV LTKNMPTNYK 2170 2180 2190 2200 2210 2220 DFITESLSVL DSWTGFKGYS LCFSKTKNTL MIHTSEGNLR LKGKLCRKLF DDPVHVEDIE

References

[1]	"Phylogeny and evolution of old world arenaviruses." Emonet S., Lemasson J.J., Gonzalez J.P., de Lamballerie X., Charrel R.N. Virology 350:251-257(2006) [PubMed: 16494913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Phylogeny of the genus Arenavirus." Charrel R.N., de Lamballerie X., Emonet S. Curr. Opin. Microbiol. 11:362-368(2008) [PubMed: 18602020] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ328876 Genomic RNA. Translation: ABC71139.1.
RefSeq	YP_516229.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3953123.
Family and domain databases
InterPro	IPR014386. RNA-dir_pol_arenavirus. IPR007099. RNA-dir_pol_NSvirus. IPR010453. RNA_pol_arenavir. [Graphical view]
Pfam	PF06317. Arena_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000836. L_ArenaV. 1 hit.
PROSITE	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_MOBVC

Accession

Primary (citable) accession number: Q27YE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	April 4, 2006
Last modified:	January 19, 2010
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)