Q27YE1 (L_IPPYV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase L
Short name=Protein L
EC=2.7.7.48

Alternative name(s):
Large structural protein
Replicase
Transcriptase

Gene names

Name:

L

Organism

Ippy virus (isolate Rat/Central African Republic/Dak An B 188 d/1970) (IPPYV) [Complete proteome]

Taxonomic identifier

55096 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Arenaviridae › Arenavirus › Old world arenaviruses

Virus host

Praomys (African soft-furred rats) [TaxID: 10111]

Protein attributes

Sequence length	2208 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Homomultimerizes; the oligomeric structure is essential for the polymerase activity. Interacts with the nucleocapsid protein N By similarity. Interacts with protein Z; this interaction inhibits viral transcription and replication By similarity.
Subcellular location	Virion. Host cytoplasm.
Sequence similarities	Belongs to the arenaviridae RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	Cap snatching RNA replication
Cellular component	Host cytoplasm Virion
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2208	2208	RNA-directed RNA polymerase L		PRO_0000361637
Regions
	Domain	1160 – 1356	197	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	Q27YE1 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: 1DFF7E36C03D7E13	FASTA	2,208	253,006
	10 20 30 40 50 60 MEESLRETKL LISRYLRQDE RIARQKLAFL GQSEPRSLLI EGLKLLSLCI EIDSCDTNCC 70 80 90 100 110 120 THNTEGQSVE NFLFQNHILC PSLPLVVPDG MKLNGNILII LECFVRSNPT NFQQKYQEDS 130 140 150 160 170 180 VKLDSLKGDL ERAGISLIPI IDGRTSYYNS LMEEWVCDQF RHNLFKLLEF EQENNALFEE 190 200 210 220 230 240 SEYLRLCESL NVSGGRASGA QGLHSLLDCR GEHYNEILKA CHIGIDPSIG GVELKGQIEN 250 260 270 280 290 300 LYQVFRQKLK KGVIKHQFRK VDQKSLLKEY CEMYKGIGIC GVEETTVDAL AAELPNISPI 310 320 330 340 350 360 LRYIHLRIDS ESNAEVNEIS NLPTGLRSAF NKVKSLKVLN TRRKLLLLID TIILMSHCYV 370 380 390 400 410 420 RELFPTLCER DWLGSSFFSV GDRLVSVGAI QHDLSKWLKR RLKANGGVGQ KSTELHKMIN 430 440 450 460 470 480 TMIQKSSKAL GDVGLSFESY GVSFDFLNKV GLEEIMRFKI VGVTPTISYI KTNQQPPIPL 490 500 510 520 530 540 REFSAEDDSD LKMLSSLSLS LVNSMKTSST VKTRQNAMGR ERYRVVQCKE CYYQELGNEY 550 560 570 580 590 600 RDLVLLYQKT GEGSKCYSVN SKRVGEICSF YADPKRYFCP IFSENVITKV IDTMMTWLMG 610 620 630 640 650 660 IVELEDSLRD IKKLTKMILL VILCQPSKRS QKLLQNLRYF IMAFVSDYHH VELFDKLREE 670 680 690 700 710 720 LITDAEFFLF KLLGKILTIL LNDEVSTMLN NRFKFILNIS YFCHFITKET PDRLTDQIKC 730 740 750 760 770 780 FEKYLEPKIQ FGSLTVNPKE TPTDEEKDDI LHGVNMFLSK KTCDEVDDPP SKKPGVSKKV 790 800 810 820 830 840 FSLMLSAFNS GLLFKESELK KGMKDPLEDS GSATALDLAS NKSVVINKYT KDGRVLDYNY 850 860 870 880 890 900 DKLVSVAVCQ LSEIFSRKGK YLLNKEDYDY KIQEVLSSLV IGSSKSEQPE EILDVDSDYM 910 920 930 940 950 960 DQLKASVERV LDQYKPNRGV RSQNNDKSVN DLKIIVEDEL SRRLILGELS YHLVEDFDKG 970 980 990 1000 1010 1020 LLSENFYKEV CEKAFNNKDF RTKYFYDSEA GLCPIEKMTQ ALATRTYMSG EYFHCFKSLL 1030 1040 1050 1060 1070 1080 LQMDANKLSG KYSHYKSQNL NFRFDHGRLM DDSRISERES NSEALSKALS LVNCLTSALK 1090 1100 1110 1120 1130 1140 NLCFYSQESP SSYTETGPDT GRMKFSLSYK EQVGGNRELY IGDLRTKMFT RFVEDYFESY 1150 1160 1170 1180 1190 1200 TKQLEGSCLN NEKEFEKAIL GMKLGVSLAH ASYSLDHSKW GPMMCPFLFL MLYRNLSPKL 1210 1220 1230 1240 1250 1260 KGTEVELKGC DNISTILSWH IHKLVEVPFN VVTAMMRSYI KRKLGIMKDT SQTITESLFF 1270 1280 1290 1300 1310 1320 SEFERGVIPS HFSSVLDMGQ GILHNTSDFY GLISERFINY ALRLVSGNPI EAYTSSDDQI 1330 1340 1350 1360 1370 1380 SLFSHKFTEL MDTDPEEFLI YLEFHNYLSS LLNKFISPKS VVGRFVAEFK SRFYVWGDEV 1390 1400 1410 1420 1430 1440 PLLSKFVAAS LHNIKCKEPH QLAETVDTII DQAVANGVPV SVCNEVQKRT LRLLEFSKYP 1450 1460 1470 1480 1490 1500 IDPFLLHSDS DVKDWVDGNR GYRIMRVIEQ TLPEGTASVR SLLRILYNKL KSNELHEEFA 1510 1520 1530 1540 1550 1560 SAYLSQNRSE TLVGLAELMG VKPPSTEDLM ICWLNLTACH PLRMVLRQKV IYPSALNLEE 1570 1580 1590 1600 1610 1620 EKVPTLIRTL QNKLSSGFTR GAQKLLSEAV NKSAFQSSIA SGFVGLCKTL GSKCVRDPER 1630 1640 1650 1660 1670 1680 ESHYIKSIIQ YLQTHCNVKP LNKGHLNLWV YESKTDDTQS ASVKPWQIEL LRPLLWDYLC 1690 1700 1710 1720 1730 1740 IALSTSLEIG PWVLGEPVFK VKSDFWKPRP CDYFPLRPAH NRILEDRIGM NHIIHAVRRL 1750 1760 1770 1780 1790 1800 YPEMFEKHLL PYMSDLAAMK LKWSPRIKFL DLCVTLDVNC EALSLISHVV KWKREEHYIV 1810 1820 1830 1840 1850 1860 LSDDLLVSHD RKHTTLMDET VVSTSDVADN FLKQIYFESF VKPFVATSRT LGSFSWFPHR 1870 1880 1890 1900 1910 1920 SSLPQGEGIE RLGPFSTFIE KVVFKGIERP MYRYDLFMGY SWLDYEIELA HLNQSQLIAS 1930 1940 1950 1960 1970 1980 GLTEESCFED VDQFWHYLST LKVGSVKLSK TVRLTQKTQG KLQGQKFSVH LNFTGFITNS 1990 2000 2010 2020 2030 2040 CTFVPKQLEV LYSGPVDEHF VIDCWSLLKS DREFKAGASE WFVHSDVVDA YISTASPSSE 2050 2060 2070 2080 2090 2100 AYPLDVWLEP DLLELSVSDI SKVGPEVNIV PLVVEDGHLL ELKEKVAIIN PVILDQDIEV 2110 2120 2130 2140 2150 2160 FINELKEDHW DLLVCKFADI LKHRQCCNLY LINVDILTIA LRILNDKAEE FISKSMQEID 2170 2180 2190 2200 QWFDFKGYSL CFSKSRRQVM RHSSTGTMRL KGRLCQPAFY VEVVEEID

References

[1]	"Phylogeny and evolution of old world arenaviruses." Emonet S., Lemasson J.J., Gonzalez J.P., de Lamballerie X., Charrel R.N. Virology 350:251-257(2006) [PubMed: 16494913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Phylogeny of the genus Arenavirus." Charrel R.N., de Lamballerie X., Emonet S. Curr. Opin. Microbiol. 11:362-368(2008) [PubMed: 18602020] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ328878 Genomic RNA. Translation: ABC71143.1.
RefSeq	YP_516233.1. NC_007906.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3953120.
Family and domain databases
InterPro	IPR014386. RNA-dir_pol_arenavirus. IPR007099. RNA-dir_pol_NSvirus. IPR010453. RNA_pol_arenavir. [Graphical view]
Pfam	PF06317. Arena_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000836. L_ArenaV. 1 hit.
PROSITE	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_IPPYV

Accession

Primary (citable) accession number: Q27YE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	April 4, 2006
Last modified:	May 31, 2011
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program