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Protein

Protein mago nashi homolog

Gene

Magoh

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms; the function is different from the established EJC assembly (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing, mRNA transport, Nonsense-mediated mRNA decay, Translation regulation, Transport

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-RNO-109688. Cleavage of Growing Transcript in the Termination Region.
R-RNO-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-RNO-72163. mRNA Splicing - Major Pathway.
R-RNO-72187. mRNA 3'-end processing.
R-RNO-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
Protein mago nashi homolog
Alternative name(s):
Mago-nashi-like proliferation-associated protein
Gene namesi
Name:Magoh
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi1305174. Magoh.

Subcellular locationi

  • Nucleus By similarity
  • Nucleus speckle By similarity
  • Cytoplasm By similarity

  • Note: Detected in granule-like structures in the dendroplasm. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Colocalizes with the core EJC, ALYREF/THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003785831 – 146Protein mago nashi homologAdd BLAST146

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ27W02.
PRIDEiQ27W02.

Expressioni

Tissue specificityi

Expressed in primary neurons.1 Publication

Gene expression databases

BgeeiENSRNOG00000012778.
GenevisibleiQ27W02. RN.

Interactioni

Subunit structurei

Heterodimer with RBM8A. Part of the mRNA splicing-dependent exon junction complex (EJC) complex; the core complex contains CASC3, EIF4A3, MAGOH and RBM8A. Interacts with PYM1; the interaction is direct and dissociates the EJC from spliced mRNAs. Identified in the spliceosome C complex (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017421.

Structurei

3D structure databases

ProteinModelPortaliQ27W02.
SMRiQ27W02.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the mago nashi family.Curated

Phylogenomic databases

eggNOGiKOG3392. Eukaryota.
ENOG4111FJ7. LUCA.
GeneTreeiENSGT00390000003156.
HOGENOMiHOG000207428.
InParanoidiQ27W02.
KOiK12877.
OMAiKEDDTNW.
OrthoDBiEOG091G0UKA.
PhylomeDBiQ27W02.
TreeFamiTF300128.

Family and domain databases

CDDicd11295. Mago_nashi. 1 hit.
Gene3Di3.30.1560.10. 1 hit.
InterProiIPR004023. Mago_nashi.
[Graphical view]
PANTHERiPTHR12638. PTHR12638. 1 hit.
PfamiPF02792. Mago_nashi. 1 hit.
[Graphical view]
SUPFAMiSSF89817. SSF89817. 1 hit.

Sequencei

Sequence statusi: Complete.

Q27W02-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESDFYLRYY VGHKGKFGHE FLEFEFRPDG KLRYANNSNY KNDVMIRKEA
60 70 80 90 100
YVHKSVMEEL KRIIDDSEIT KEDDALWPPP DRVGRQELEI VIGDEHISFT
110 120 130 140
TSKIGSLIDV NQSKDPEGLR VFYYLVQDLK CLVFSLIGLH FKIKPI
Length:146
Mass (Da):17,164
Last modified:April 4, 2006 - v1
Checksum:iFFAD0B075E045875
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ359101 mRNA. Translation: ABD46660.1.
RefSeqiNP_001094006.1. NM_001100536.1.
UniGeneiRn.8332.

Genome annotation databases

EnsembliENSRNOT00000017422; ENSRNOP00000017421; ENSRNOG00000012778.
GeneIDi298385.
KEGGirno:298385.
UCSCiRGD:1305174. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ359101 mRNA. Translation: ABD46660.1.
RefSeqiNP_001094006.1. NM_001100536.1.
UniGeneiRn.8332.

3D structure databases

ProteinModelPortaliQ27W02.
SMRiQ27W02.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017421.

Proteomic databases

PaxDbiQ27W02.
PRIDEiQ27W02.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000017422; ENSRNOP00000017421; ENSRNOG00000012778.
GeneIDi298385.
KEGGirno:298385.
UCSCiRGD:1305174. rat.

Organism-specific databases

CTDi4116.
RGDi1305174. Magoh.

Phylogenomic databases

eggNOGiKOG3392. Eukaryota.
ENOG4111FJ7. LUCA.
GeneTreeiENSGT00390000003156.
HOGENOMiHOG000207428.
InParanoidiQ27W02.
KOiK12877.
OMAiKEDDTNW.
OrthoDBiEOG091G0UKA.
PhylomeDBiQ27W02.
TreeFamiTF300128.

Enzyme and pathway databases

ReactomeiR-RNO-109688. Cleavage of Growing Transcript in the Termination Region.
R-RNO-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-RNO-72163. mRNA Splicing - Major Pathway.
R-RNO-72187. mRNA 3'-end processing.
R-RNO-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

PROiQ27W02.

Gene expression databases

BgeeiENSRNOG00000012778.
GenevisibleiQ27W02. RN.

Family and domain databases

CDDicd11295. Mago_nashi. 1 hit.
Gene3Di3.30.1560.10. 1 hit.
InterProiIPR004023. Mago_nashi.
[Graphical view]
PANTHERiPTHR12638. PTHR12638. 1 hit.
PfamiPF02792. Mago_nashi. 1 hit.
[Graphical view]
SUPFAMiSSF89817. SSF89817. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMGN_RAT
AccessioniPrimary (citable) accession number: Q27W02
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: April 4, 2006
Last modified: November 2, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.