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Protein

Protein mago nashi homolog

Gene

Magoh

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms; the function is different from the established EJC assembly (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing, mRNA transport, Nonsense-mediated mRNA decay, Translation regulation, Transport

Enzyme and pathway databases

ReactomeiR-RNO-109688 Cleavage of Growing Transcript in the Termination Region
R-RNO-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-RNO-72163 mRNA Splicing - Major Pathway
R-RNO-72187 mRNA 3'-end processing
R-RNO-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
Protein mago nashi homolog
Alternative name(s):
Mago-nashi-like proliferation-associated protein
Gene namesi
Name:Magoh
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi1305174 Magoh

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003785831 – 146Protein mago nashi homologAdd BLAST146

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ27W02
PRIDEiQ27W02

Expressioni

Tissue specificityi

Expressed in primary neurons.1 Publication

Gene expression databases

BgeeiENSRNOG00000012778
GenevisibleiQ27W02 RN

Interactioni

Subunit structurei

Heterodimer with RBM8A. Part of the mRNA splicing-dependent exon junction complex (EJC) complex; the core complex contains CASC3, EIF4A3, MAGOH and RBM8A. Interacts with PYM1; the interaction is direct and dissociates the EJC from spliced mRNAs. Identified in the spliceosome C complex (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017421

Structurei

3D structure databases

ProteinModelPortaliQ27W02
SMRiQ27W02
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the mago nashi family.Curated

Phylogenomic databases

eggNOGiKOG3392 Eukaryota
ENOG4111FJ7 LUCA
GeneTreeiENSGT00390000003156
HOGENOMiHOG000207428
InParanoidiQ27W02
KOiK12877
OMAiDRVGRQX
OrthoDBiEOG091G0UKA
PhylomeDBiQ27W02
TreeFamiTF300128

Family and domain databases

CDDicd11295 Mago_nashi, 1 hit
Gene3Di3.30.1560.10, 1 hit
InterProiView protein in InterPro
IPR004023 Mago_nashi
IPR036605 Mago_nashi_sf
PANTHERiPTHR12638 PTHR12638, 1 hit
PfamiView protein in Pfam
PF02792 Mago_nashi, 1 hit
SUPFAMiSSF89817 SSF89817, 1 hit

Sequencei

Sequence statusi: Complete.

Q27W02-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESDFYLRYY VGHKGKFGHE FLEFEFRPDG KLRYANNSNY KNDVMIRKEA
60 70 80 90 100
YVHKSVMEEL KRIIDDSEIT KEDDALWPPP DRVGRQELEI VIGDEHISFT
110 120 130 140
TSKIGSLIDV NQSKDPEGLR VFYYLVQDLK CLVFSLIGLH FKIKPI
Length:146
Mass (Da):17,164
Last modified:April 4, 2006 - v1
Checksum:iFFAD0B075E045875
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ359101 mRNA Translation: ABD46660.1
RefSeqiNP_001094006.1, NM_001100536.1
UniGeneiRn.8332

Genome annotation databases

EnsembliENSRNOT00000017422; ENSRNOP00000017421; ENSRNOG00000012778
GeneIDi298385
KEGGirno:298385
UCSCiRGD:1305174 rat

Similar proteinsi

Entry informationi

Entry nameiMGN_RAT
AccessioniPrimary (citable) accession number: Q27W02
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: April 4, 2006
Last modified: May 23, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health