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Q27W01 (RBM8A_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA-binding protein 8A
Alternative name(s):
RNA-binding motif protein 8A
Ribonucleoprotein RBM8A
Gene names
Name:Rbm8a
Synonyms:Rbm8
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length174 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator WIBG/PYM leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. The MAGOH-RBM8A heterodimer is a component of the nonsense mediated decay (NMD) pathway. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms; the function is different from the established EJC assembly By similarity.

Subunit structure

Heterodimer with RBM8A. Part of the mRNA splicing-dependent exon junction complex (EJC) complex; the core complex contains CASC3, EIF4A3, MAGOH and RBM8A. Interacts with WIBG/PYM; the interaction is direct and dissociates the EJC from spliced mRNAs. Found in a post-splicing complex with NXF1, RBM8A, UPF1, UPF2, UPF3A, UPF3B and RNPS1. Interacts with BAT1, MAGOH, OVCA1, UPF3B, RNPS1, SRRM1 and ALYREF/THOC4. Interacts with IPO13; the interaction mediates the nuclear import of the MAGOH-RBM8A heterodimer. Identified in the spliceosome C complex. Associates with polysomes By similarity.

Subcellular location

Nucleus By similarity. Nucleus speckle By similarity. Cytoplasm By similarity. Note: Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Colocalizes with the core EJC, ALYREF/THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles By similarity.

Sequence similarities

Belongs to the RBM8A family.

Contains 1 RRM (RNA recognition motif) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 174173RNA-binding protein 8A
PRO_0000378563

Regions

Domain73 – 15179RRM
Compositional bias171 – 1744Poly-Arg

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue241Phosphoserine By similarity
Modified residue421Phosphoserine By similarity
Modified residue561Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q27W01 [UniParc].

Last modified April 4, 2006. Version 1.
Checksum: 70BBD03CDDFEECFE

FASTA17419,889
        10         20         30         40         50         60 
MADVLDLHEA GGEDFAMDED GDESIHKLKE KAKKRKGRGF GSEEGSRARM REDYDSVEQD 

        70         80         90        100        110        120 
GDEPGPQRSV EGWILFVTGV HEEATEEDIH DKFAEYGEIK NIHLNLDRRT GYLKGYTLVE 

       130        140        150        160        170 
YETYKEAQAA MEGLNGQDLM GQPISVDWCF VRGPPKGKRR GGRRRSRSPD RRRR 

« Hide

References

[1]"RNA splicing capability of live neuronal dendrites."
Glanzer J., Miyashiro K.Y., Sul J.-Y., Barrett L., Belt B., Haydon P., Eberwine J.
Proc. Natl. Acad. Sci. U.S.A. 102:16859-16864(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Hippocampus.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ359102 mRNA. Translation: ABD46661.1.
RefSeqNP_001258067.1. NM_001271138.1.
UniGeneRn.37716.

3D structure databases

ProteinModelPortalQ27W01.
SMRQ27W01. Positions 12-155.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid254951. 1 interaction.
STRING10116.ENSRNOP00000028807.

PTM databases

PhosphoSiteQ27W01.

Proteomic databases

PaxDbQ27W01.
PRIDEQ27W01.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000028807; ENSRNOP00000028807; ENSRNOG00000021215.
GeneID295284.
KEGGrno:295284.
UCSCRGD:1310099. rat.

Organism-specific databases

CTD9939.
RGD1310099. Rbm8a.

Phylogenomic databases

eggNOGCOG0724.
GeneTreeENSGT00730000111185.
HOGENOMHOG000183826.
InParanoidQ27W01.
KOK12876.
OMAWILFVTS.
OrthoDBEOG7C5MB6.
PhylomeDBQ27W01.
TreeFamTF314933.

Gene expression databases

GenevestigatorQ27W01.

Family and domain databases

Gene3D3.30.70.330. 1 hit.
InterProIPR012677. Nucleotide-bd_a/b_plait.
IPR008111. RNA-bd_8.
IPR000504. RRM_dom.
[Graphical view]
PfamPF00076. RRM_1. 1 hit.
[Graphical view]
PRINTSPR01738. RNABINDINGM8.
SMARTSM00360. RRM. 1 hit.
[Graphical view]
PROSITEPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio639304.
PROQ27W01.

Entry information

Entry nameRBM8A_RAT
AccessionPrimary (citable) accession number: Q27W01
Entry history
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: April 4, 2006
Last modified: April 16, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families