Q27W01 (RBM8A_RAT) Reviewed, UniProtKB/Swiss-Prot
Last modified December 11, 2013. Version 60. History...
Names and origin
|Protein names||Recommended name:|
RNA-binding protein 8A
RNA-binding motif protein 8A
|Organism||Rattus norvegicus (Rat) [Reference proteome]|
|Taxonomic identifier||10116 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus|
|Sequence length||174 AA.|
|Sequence processing||The displayed sequence is further processed into a mature form.|
|Protein existence||Evidence at transcript level|
General annotation (Comments)
Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The heterodimer MAGOH-RBM8A interacts with PYM that function to enhance the translation of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Remains associated with mRNAs in the cytoplasm until the mRNAs engage the translation machinery. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. Complex with MAGOH is a component of the nonsense mediated decay (NMD) pathway By similarity.
Heterodimer with MAGOH. Part of the exon junction complex (EJC) core complex that contains CASC3, EIF4A3, MAGOH and RBM8A. Found in a mRNA splicing-dependent EJC, at least composed of ACIN1, CASC3, DEK, EIF4A3, MAGOH, NCBP1, NCBP2, PNN, NXF1, RBM8A, UPF2, UPF3A, UPF3B, RNPS1, SAP18, SRRM1 and ALYREF/THOC4. Found in a post-splicing complex with NXF1, RBM8A, UPF1, UPF2, UPF3A, UPF3B and RNPS1. Interacts with BAT1, MAGOH, OVCA1, UPF3B, RNPS1, SRRM1 and ALYREF/THOC4. Identified in the spliceosome C complex. Interacts with WIBG/PYM; the interaction is direct and leads to dissociate the EJC from spliced mRNAs. Associates with polysomes By similarity.
Nucleus By similarity. Nucleus speckle By similarity. Cytoplasm By similarity. Note: Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Colocalizes with the core EJC, ALYREF/THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles By similarity.
Belongs to the RBM8A family.
Contains 1 RRM (RNA recognition motif) domain.
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Initiator methionine||1||1||Removed By similarity|
|Chain||2 – 174||173||RNA-binding protein 8A||PRO_0000378563|
|Domain||73 – 151||79||RRM|
|Compositional bias||171 – 174||4||Poly-Arg|
Amino acid modifications
|Modified residue||2||1||N-acetylalanine By similarity|
|Modified residue||24||1||Phosphoserine By similarity|
|Modified residue||42||1||Phosphoserine By similarity|
|Modified residue||56||1||Phosphoserine By similarity|
|||"RNA splicing capability of live neuronal dendrites."|
Glanzer J., Miyashiro K.Y., Sul J.-Y., Barrett L., Belt B., Haydon P., Eberwine J.
Proc. Natl. Acad. Sci. U.S.A. 102:16859-16864(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
|DQ359102 mRNA. Translation: ABD46661.1.|
|RefSeq||NP_001258067.1. NM_001271138.1. |
3D structure databases
|SMR||Q27W01. Positions 12-155. |
Protein-protein interaction databases
Protocols and materials databases
Genome annotation databases
|Ensembl||ENSRNOT00000028807; ENSRNOP00000028807; ENSRNOG00000021215. |
|UCSC||RGD:1310099. rat. |
|RGD||1310099. Rbm8a. |
Gene expression databases
Family and domain databases
|Gene3D||220.127.116.110. 1 hit. |
|InterPro||IPR012677. Nucleotide-bd_a/b_plait. |
|Pfam||PF00076. RRM_1. 1 hit. |
|PRINTS||PR01738. RNABINDINGM8. |
|SMART||SM00360. RRM. 1 hit. |
|PROSITE||PS50102. RRM. 1 hit. |
|Accession||Primary (citable) accession number: Q27W01|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|
Index of protein domains and families