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Q27W01

- RBM8A_RAT

UniProt

Q27W01 - RBM8A_RAT

Protein

RNA-binding protein 8A

Gene

Rbm8a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
  1. Functioni

    Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator WIBG/PYM leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. The MAGOH-RBM8A heterodimer is a component of the nonsense mediated decay (NMD) pathway. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms; the function is different from the established EJC assembly By similarity.By similarity

    GO - Molecular functioni

    1. nucleotide binding Source: InterPro
    2. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. mRNA processing Source: UniProtKB-KW
    2. mRNA transport Source: UniProtKB-KW
    3. nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB-KW
    4. regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
    5. regulation of translation Source: UniProtKB-KW
    6. RNA splicing Source: UniProtKB-KW

    Keywords - Biological processi

    mRNA processing, mRNA splicing, mRNA transport, Nonsense-mediated mRNA decay, Translation regulation, Transport

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_208719. mRNA Splicing - Major Pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA-binding protein 8A
    Alternative name(s):
    RNA-binding motif protein 8A
    Ribonucleoprotein RBM8A
    Gene namesi
    Name:Rbm8a
    Synonyms:Rbm8
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 2

    Organism-specific databases

    RGDi1310099. Rbm8a.

    Subcellular locationi

    Nucleus By similarity. Nucleus speckle By similarity. Cytoplasm By similarity
    Note: Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Colocalizes with the core EJC, ALYREF/THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles By similarity.By similarity

    GO - Cellular componenti

    1. catalytic step 2 spliceosome Source: Ensembl
    2. cytoplasm Source: UniProtKB-SubCell
    3. exon-exon junction complex Source: Ensembl
    4. nuclear speck Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Spliceosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 174173RNA-binding protein 8APRO_0000378563Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei24 – 241PhosphoserineBy similarity
    Modified residuei42 – 421PhosphoserineBy similarity
    Modified residuei56 – 561PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ27W01.
    PRIDEiQ27W01.

    PTM databases

    PhosphoSiteiQ27W01.

    Expressioni

    Gene expression databases

    GenevestigatoriQ27W01.

    Interactioni

    Subunit structurei

    Heterodimer with RBM8A. Part of the mRNA splicing-dependent exon junction complex (EJC) complex; the core complex contains CASC3, EIF4A3, MAGOH and RBM8A. Interacts with WIBG/PYM; the interaction is direct and dissociates the EJC from spliced mRNAs. Found in a post-splicing complex with NXF1, RBM8A, UPF1, UPF2, UPF3A, UPF3B and RNPS1. Interacts with BAT1, MAGOH, OVCA1, UPF3B, RNPS1, SRRM1 and ALYREF/THOC4. Interacts with IPO13; the interaction mediates the nuclear import of the MAGOH-RBM8A heterodimer. Identified in the spliceosome C complex. Associates with polysomes By similarity.By similarity

    Protein-protein interaction databases

    BioGridi254951. 1 interaction.
    STRINGi10116.ENSRNOP00000028807.

    Structurei

    3D structure databases

    ProteinModelPortaliQ27W01.
    SMRiQ27W01. Positions 12-155.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini73 – 15179RRMPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi171 – 1744Poly-Arg

    Sequence similaritiesi

    Belongs to the RBM8A family.Curated
    Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0724.
    GeneTreeiENSGT00730000111185.
    HOGENOMiHOG000183826.
    InParanoidiQ27W01.
    KOiK12876.
    OMAiWILFVTS.
    OrthoDBiEOG7C5MB6.
    PhylomeDBiQ27W01.
    TreeFamiTF314933.

    Family and domain databases

    Gene3Di3.30.70.330. 1 hit.
    InterProiIPR012677. Nucleotide-bd_a/b_plait.
    IPR008111. RNA-bd_8.
    IPR000504. RRM_dom.
    [Graphical view]
    PfamiPF00076. RRM_1. 1 hit.
    [Graphical view]
    PRINTSiPR01738. RNABINDINGM8.
    SMARTiSM00360. RRM. 1 hit.
    [Graphical view]
    PROSITEiPS50102. RRM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q27W01-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADVLDLHEA GGEDFAMDED GDESIHKLKE KAKKRKGRGF GSEEGSRARM    50
    REDYDSVEQD GDEPGPQRSV EGWILFVTGV HEEATEEDIH DKFAEYGEIK 100
    NIHLNLDRRT GYLKGYTLVE YETYKEAQAA MEGLNGQDLM GQPISVDWCF 150
    VRGPPKGKRR GGRRRSRSPD RRRR 174
    Length:174
    Mass (Da):19,889
    Last modified:April 4, 2006 - v1
    Checksum:i70BBD03CDDFEECFE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ359102 mRNA. Translation: ABD46661.1.
    RefSeqiNP_001258067.1. NM_001271138.1.
    UniGeneiRn.37716.

    Genome annotation databases

    EnsembliENSRNOT00000028807; ENSRNOP00000028807; ENSRNOG00000021215.
    GeneIDi295284.
    KEGGirno:295284.
    UCSCiRGD:1310099. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ359102 mRNA. Translation: ABD46661.1 .
    RefSeqi NP_001258067.1. NM_001271138.1.
    UniGenei Rn.37716.

    3D structure databases

    ProteinModelPortali Q27W01.
    SMRi Q27W01. Positions 12-155.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 254951. 1 interaction.
    STRINGi 10116.ENSRNOP00000028807.

    PTM databases

    PhosphoSitei Q27W01.

    Proteomic databases

    PaxDbi Q27W01.
    PRIDEi Q27W01.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000028807 ; ENSRNOP00000028807 ; ENSRNOG00000021215 .
    GeneIDi 295284.
    KEGGi rno:295284.
    UCSCi RGD:1310099. rat.

    Organism-specific databases

    CTDi 9939.
    RGDi 1310099. Rbm8a.

    Phylogenomic databases

    eggNOGi COG0724.
    GeneTreei ENSGT00730000111185.
    HOGENOMi HOG000183826.
    InParanoidi Q27W01.
    KOi K12876.
    OMAi WILFVTS.
    OrthoDBi EOG7C5MB6.
    PhylomeDBi Q27W01.
    TreeFami TF314933.

    Enzyme and pathway databases

    Reactomei REACT_208719. mRNA Splicing - Major Pathway.

    Miscellaneous databases

    NextBioi 639304.
    PROi Q27W01.

    Gene expression databases

    Genevestigatori Q27W01.

    Family and domain databases

    Gene3Di 3.30.70.330. 1 hit.
    InterProi IPR012677. Nucleotide-bd_a/b_plait.
    IPR008111. RNA-bd_8.
    IPR000504. RRM_dom.
    [Graphical view ]
    Pfami PF00076. RRM_1. 1 hit.
    [Graphical view ]
    PRINTSi PR01738. RNABINDINGM8.
    SMARTi SM00360. RRM. 1 hit.
    [Graphical view ]
    PROSITEi PS50102. RRM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Hippocampus.

    Entry informationi

    Entry nameiRBM8A_RAT
    AccessioniPrimary (citable) accession number: Q27W01
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 7, 2009
    Last sequence update: April 4, 2006
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3