Q27GR6 (Q27GR6_ACTS5) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Acarbose resistent alpha-amylase AcbE EMBL CAJ81030.1
EC=3.2.1.1 EMBL CAJ81030.1

Gene names

Name:

acbE EMBL CAJ81030.1

Organism

Actinoplanes sp. (strain 50/110) EMBL CAJ81030.1

Taxonomic identifier

134676 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micromonosporineae › Micromonosporaceae › Actinoplanes

Protein attributes

Sequence length	1038 AA.
Sequence status	Complete.
Protein existence	Predicted

Ontologies

Keywords
Molecular function	Glycosidase EMBL CAJ81030.1 Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	alpha-amylase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q27GR6 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: ACC65E1E427D75B7	FASTA	1,038	112,577
	10 20 30 40 50 60 MDERLKSHIV RALLPVLVGA AGGLPAPRAA QAESPPDRPS HAEQLYLVMP DRFANGDTGN 70 80 90 100 110 120 DQGGLTGDRL TTGYDPTDAG FYHGGDIQGV IDKLDYIQGL GTTAIWLTPI FKNKPVQGTG 130 140 150 160 170 180 PNASAGYHGY WITDFTQVDP HFGTNEDLKR LVGLAHRRGM KIYLDIVVNH TADVIKNAEG 190 200 210 220 230 240 TAYADKGTAP YRDTAGRPFE DRNYADGTHG FPKVDEKSFP HRPFFEKPSD ATAKAPAWLN 250 260 270 280 290 300 DPTMYHNRGE TTFGGENAEY GDFTGGLDDL WTERPEVLHG LTDIYAGWIR RFGVDGYRLD 310 320 330 340 350 360 TLKATDLAFW RPFAEGVGKA ADKAGKRDFF LFGEAWSKDQ EVTSTFVRRG GLPATLDFPF 370 380 390 400 410 420 QGAAQSYARG QGTGQQPWQD LYAKDDLYSR RPGPTPAGCH LGEWNHDMGR IATLIKAGSD 430 440 450 460 470 480 PKRGCRLELV NELMFLTRGQ PGGLLRADEQ GFTGRPRLNK GSRQDMFASR TPDYLDDRLI 490 500 510 520 530 540 GTDKTHASDN HDTGHPLYRT IAELGAVRKA HPGLRDGEQI TRYAADRAGV FAASRIDRDQ 550 560 570 580 590 600 RAEYVIAVNN ATTAQTVTFD TYTPGCGFQP LYGTGETAAT GADGRLTITV PALSAVAYRA 610 620 630 640 650 660 EKPIPAADGA PAVTITAPAG RDPRAGPHRT DRAVTGDPLS TVTFSARAGN GPWQPIGTAG 670 680 690 700 710 720 RGPYRVFHDL TGLAGGAEVQ YRRSPGTPRP YRHHHQPDPV GTVRAEAGYA VVHYQRPAGD 730 740 750 760 770 780 YDGWRLHATG ERSKRPRSNP QGEPFTGNGT PNGPFAWVKL RPGATKVTFT VSDANGVKDV 790 800 810 820 830 840 DADRSLNPQQ TPGIWLHQGD PAISYQGPAP APAPNTAILH YRRPAGDYTG WGLHLWDGAA 850 860 870 880 890 900 TPTADWGAPL LPESFDSFGA VFRIPLAAGA TGLSYIMHAG EAKDLPTDQR LEFADAGREV 910 920 930 940 950 960 WVQAGVQERL LPSVVKPAKL DLTRADAVRV DRDTIAWQTG TPDTLEPAGA ATDGRVYDLV 970 980 990 1000 1010 1020 HAPNGGLRLV DGDLAGDYRS IRLTAQRDGL TEKQRRQAPE LWQYPAFQAA AALPSGDGQF 1030 YVTERDAQGR LMSVTGVR

References

[1]	"The AcbC protein from Actinoplanes species is a C7-cyclitol synthase related to 3-dehydroquinate synthases and is involved in the biosynthesis of the alpha-glucosidase inhibitor acarbose." Stratmann A., Mahmud T., Lee S., Distler J., Floss H.G., Piepersberg W. J. Biol. Chem. 274:10889-10896(1999) [PubMed: 10196166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: SE50/110 EMBL CAJ81030.1.
[2]	"Identification, cloning, expression, and characterization of the extracellular acarbose-modifying glycosyltransferase, AcbD, from Actinoplanes sp. strain SE50." Hemker M., Stratmann A., Goeke K., Schroder W., Lenz J., Piepersberg W., Pape H. J. Bacteriol. 183:4484-4492(2001) [PubMed: 11443082] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: SE50/110 EMBL CAJ81030.1.
[3]	"Biosynthesis of the C(7)-cyclitol moiety of acarbose in Actinoplanes species SE50/110. 7-O-phosphorylation of the initial cyclitol precursor leads to proposal of a new biosynthetic pathway." Zhang C.S., Stratmann A., Block O., Bruckner R., Podeschwa M., Altenbach H.J., Wehmeier U.F., Piepersberg W. J. Biol. Chem. 277:22853-22862(2002) [PubMed: 11937512] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: SE50/110 EMBL CAJ81030.1.
[4]	"The acarbose-biosynthetic enzyme AcbO from Actinoplanes sp. SE 50/110 is a 2-epi-5-epi-valiolone-7-phosphate 2-epimerase." Zhang C.S., Podeschwa M., Altenbach H.J., Piepersberg W., Wehmeier U.F. FEBS Lett. 540:47-52(2003) [PubMed: 12681481] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: SE50/110 EMBL CAJ81030.1.
[5]	"The acbH gene of Actinoplanes sp. encodes a solute receptor with binding activities for acarbose and longer homologs." Brunkhorst C., Wehmeier U.F., Piepersberg W., Schneider E. Res. Microbiol. 156:322-327(2005) [PubMed: 15808935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: SE50/110 EMBL CAJ81030.1.
[6]	Stratmann A. Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: SE50/110 EMBL CAJ81030.1.
[7]	Wehmeier U.F. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: SE50/110 EMBL CAJ81030.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y18523 Genomic DNA. Translation: CAJ81030.1.
3D structure databases
ProteinModelPortal	Q27GR6.
ModBase	Search...
Protein family/group databases
CAZy	CBM41. Carbohydrate-Binding Module Family 41. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR006589. Glyco_hydro_13_sub_cat_dom. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR005323. PUD. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10357. Alpha_amylase. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF03714. PUD. 2 hits. [Graphical view]
SMART	SM00642. Aamy. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q27GR6_ACTS5

Accession

Primary (citable) accession number: Q27GR6

Entry history

Integrated into UniProtKB/TrEMBL:	April 4, 2006
Last sequence update:	April 4, 2006
Last modified:	September 21, 2011
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)