Reviewed, UniProtKB/Swiss-Prot Q27996 (LYSCT_BOVIN)

Names and origin

Protein names	Recommended name:     Lysozyme C, tracheal isozyme     EC=3.2.1.17 Alternative name(s):     1,4-beta-N-acetylmuramidase C
Organism	Bos taurus (Bovine)
Taxonomic identifier	9913 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Subunit structure	Monomer.
Tissue specificity	Trachea.
Miscellaneous	Lysozyme C is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. It acts rapidly on both peptide-substituted and unsubstituted peptidoglycan, and slowly on chitin oligosaccharides. The ruminant gastric lysozymes, which digest symbiotic bacteria coming with cud from the rumen, are much more resistant to inactivation by pepsin than are other lysozymes.
Sequence similarities	Belongs to the glycosyl hydrolase 22 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	By similarity
	Chain	19 – 147	129	Lysozyme C, tracheal isozyme		PRO_0000018457
Sites
	Active site	53	1	By similarity
	Active site	71	1	By similarity
Amino acid modifications
	Disulfide bond	24 ↔ 145		By similarity
	Disulfide bond	48 ↔ 133		By similarity
	Disulfide bond	83 ↔ 99		By similarity
	Disulfide bond	95 ↔ 113		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q27996-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 659AD5E5167BDDDD	FASTA	147	15,929
	10 20 30 40 50 60 MKALLILGLL LLSVAVQGKT FKRCELAKTL KNLGLAGYKG VSLANWMCLA KGESNYNTQA 70 80 90 100 110 120 KNYNPGSKST DYGIFQINSK WWCNDGKTPK AVNGCGVSCS ALLKDDITQA VACAKKIVSQ 130 140 QGITAWVAWK NKCRNRDLTS YVKGCGV

References

[1]	"Evolution of the bovine lysozyme gene family: changes in gene expression and reversion of function." Irwin D.M. J. Mol. Evol. 41:299-312(1995) [PubMed: 7563116] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U19466 Genomic DNA. Translation: AAA85544.1.
IPI	IPI00717428.
PIR	B49315.
RefSeq	NP_001073805.1.
UniGene	Bt.64327
3D structure databases
SMR	Q27996. Positions 19-145.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000000231; ENSBTAP00000000231; ENSBTAG00000000198; Bos taurus. [Genome view] ENSBTAT00000053457; ENSBTAP00000048332; ENSBTAG00000000198; Bos taurus. [Genome view]
GeneID	781146.
KEGG	bta:781146.
Phylogenomic databases
eggNOG	maNOG19640.
HOVERGEN	Q27996.
InParanoid	Q27996.
OMA	SECELAK.
OrthoDB	EOG9MD0K1.
Enzyme and pathway databases
BRENDA	3.2.1.17. 251.
Family and domain databases
InterPro	IPR001916. Glyco_hydro_22. IPR019799. Glyco_hydro_22_CS. IPR000974. Glyco_hydro_22_lys. [Graphical view]
Pfam	PF00062. Lys. 1 hit. [Graphical view]
PRINTS	PR00137. LYSOZYME. PR00135. LYZLACT.
SMART	SM00263. LYZ1. 1 hit. [Graphical view]
PROSITE	PS00128. LACTALBUMIN_LYSOZYME_1. 1 hit. PS51348. LACTALBUMIN_LYSOZYME_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYSCT_BOVIN

Accession

Primary (citable) accession number: Q27996

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 27, 2003
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)