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Q27995

- NOS2_BOVIN

UniProt

Q27995 - NOS2_BOVIN

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Protein

Nitric oxide synthase, inducible

Gene

NOS2

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 (By similarity).By similarity

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Protein has several cofactor binding sites:
  • hemeBy similarity
  • FADBy similarityNote: Binds 1 FAD.By similarity
  • FMNBy similarityNote: Binds 1 FMN.By similarity
  • 5,6,7,8-tetrahydrobiopterinBy similarityNote: Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.By similarity

Enzyme regulationi

Regulated by calcium/calmodulin.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi200 – 2001Iron (heme axial ligand)By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi623 – 65432FMNPROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi767 – 77812FADBy similarityAdd
BLAST
Nucleotide bindingi903 – 91311FADBy similarityAdd
BLAST
Nucleotide bindingi978 – 99619NADPBy similarityAdd
BLAST
Nucleotide bindingi1076 – 109116NADPBy similarityAdd
BLAST

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: InterPro
  2. FMN binding Source: InterPro
  3. heme binding Source: InterPro
  4. iron ion binding Source: InterPro
  5. NADP binding Source: InterPro
  6. nitric-oxide synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. nitric oxide biosynthetic process Source: InterPro
  2. peptidyl-cysteine S-nitrosylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calcium, Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase, inducible (EC:1.14.13.39)
Alternative name(s):
Inducible NO synthase
Short name:
Inducible NOS
Short name:
iNOS
NOS type II
Short name:
NOSII
Peptidyl-cysteine S-nitrosylase NOS2
Gene namesi
Name:NOS2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11561156Nitric oxide synthase, induciblePRO_0000170926Add
BLAST

Proteomic databases

PRIDEiQ27995.

Interactioni

Subunit structurei

Homodimer. Binds SLC9A3R1 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ27995.
SMRiQ27995. Positions 83-502, 703-1130.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini539 – 677139Flavodoxin-likePROSITE-ProRule annotationAdd
BLAST
Domaini730 – 970241FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni509 – 52921Calmodulin-bindingSequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the NOS family.Curated
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG4362.
HOGENOMiHOG000220884.
HOVERGENiHBG000159.
InParanoidiQ27995.
KOiK13241.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
3.90.340.10. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000333. NOS. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q27995-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MACPWQFLFK IKSQKVDLAT ELDINNNVGK FYQPPSSPVT QDDPKRHSPG
60 70 80 90 100
KHGNESPQPL TGTVKTSPES LSKLDAPPSA CPRHVRIKNW GSGVTFQDTL
110 120 130 140 150
HQKAKGDLSC KSKSCLASIM NPKSLTIGPR DKPTPPDELL PQAIEFVNQY
160 170 180 190 200
YGSFKEAKIE EHLARVEAVT KEIETTGTYQ LTGDELIFAT KQAWRNAPRC
210 220 230 240 250
IGRIQWSNLQ VFDARSCSTA QEMFEHICRH VRYATNNGNI RSAITVFPQR
260 270 280 290 300
SDGKHDFRVW NAQLIRYAGY QMPDGSIRGD PANVEFTQLC IDLGWKPKYG
310 320 330 340 350
RFDVLPLVLQ ADGRDPELFE IPPDLVLEVP MEHPRYEWFR ELELKWYALP
360 370 380 390 400
AVANMLLEVG GLEFPGCPFN GWYMGTEVGV RDFCDAQRYN ILEEVGRRMG
410 420 430 440 450
LETHKVASLW KDRAVVEINV AVLHSFQKQN VTIMDHHSAA ESFMKYMQNE
460 470 480 490 500
YRSRGGCPAD WIWLVPPISG SITPVFHQEM LNYVLSPFYY YQVEPWKTHV
510 520 530 540 550
WQDERRRPQR REIRFKVLVK AVFFASVLMH KAMASRVRAT ILFATETGRS
560 570 580 590 600
ETLAQDLGAL FSCAFNPKVL CMDQYQLSHL EEEQLLLVVT STFGNGDSPG
610 620 630 640 650
NGEKLKKSLL MLKELTNTFR YAVFGLGSSM YPQFCAFAHD IDQKLSQLGA
660 670 680 690 700
SQLAPTGEGD ELSGQEEAFR SWAVQTFKAA CETFDVSGKH HIEIPKLYTS
710 720 730 740 750
NVTWDPQHYR LVQDSEPLDL NKALSSMHAK HVFTMRLKSQ QNLQSPKSSR
760 770 780 790 800
TTLLVELSCE GSQAPSYLPG EHLGVFPCNQ PALVQGILER VVDGPAPHQP
810 820 830 840 850
VRLETLCENG SYWVKDKRLP PCSLSQALTY FLDITTPPTQ LLLRKLAQLA
860 870 880 890 900
TEEAEKQRLE TLCQPSDYNK WKFTNSPTFL EVLEEFPSLR VSASFLLSQL
910 920 930 940 950
PILKPRYYSI SSSRDLTPTE IHLTVAVLTY RTRDGQGPLH HGVCSTWLSS
960 970 980 990 1000
LKPQDPVPCF VRSASGFQLP EDRSRPCILI GPGTGIAPFR SFWQQRLHEA
1010 1020 1030 1040 1050
EHKGLQGGRM TLVFGCRRPE EDHLYWEEML EMARKGVLHE VHTAYSRLPD
1060 1070 1080 1090 1100
QPKVYVQDIL RQRLAGEVLR VLHEEQGHLY VCGDVRMARD VARTLKQLMA
1110 1120 1130 1140 1150
TALSLNEEQV EDYFFQLKNQ KRYHEDIFGA VFPYEVKKDG AAGLPSNPRA

PGAHRS
Length:1,156
Mass (Da):131,208
Last modified:November 28, 2006 - v3
Checksum:i52A52C984462F692
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti236 – 2361N → S in AAC48470. (PubMed:7536776)Curated
Sequence conflicti1068 – 10692VL → AF in AAC48479. (PubMed:7558036)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ676956 mRNA. Translation: ABG74910.1.
U14640 mRNA. Translation: AAC48470.2.
U18331 mRNA. Translation: AAC48479.1.
PIRiI46074.
RefSeqiNP_001070267.1. NM_001076799.1.
UniGeneiBt.23126.

Genome annotation databases

GeneIDi282876.
KEGGibta:282876.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ676956 mRNA. Translation: ABG74910.1 .
U14640 mRNA. Translation: AAC48470.2 .
U18331 mRNA. Translation: AAC48479.1 .
PIRi I46074.
RefSeqi NP_001070267.1. NM_001076799.1.
UniGenei Bt.23126.

3D structure databases

ProteinModelPortali Q27995.
SMRi Q27995. Positions 83-502, 703-1130.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q27995.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 282876.
KEGGi bta:282876.

Organism-specific databases

CTDi 4843.

Phylogenomic databases

eggNOGi COG4362.
HOGENOMi HOG000220884.
HOVERGENi HBG000159.
InParanoidi Q27995.
KOi K13241.

Miscellaneous databases

NextBioi 20806404.

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
3.90.340.10. 1 hit.
InterProi IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000333. NOS. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of bovine inducible nitric oxide synthase."
    Widdison S., Ashley G.R., Howard C.J., Coffey T.J.
    Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Inducible nitric oxide synthase in cattle. Differential cytokine regulation of nitric oxide synthase in bovine and murine macrophages."
    Adler H., Frech B., Thoeny M., Pfister H., Peterhans E., Jungi T.W.
    J. Immunol. 154:4710-4718(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 166-275.
    Tissue: Bone marrow macrophage.
  3. "Three members of the nitric oxide synthase II gene family (NOS2A, NOS2B, and NOS2C) colocalize to human chromosome 17."
    Bloch K.D., Wolfram J.R., Brown D.M., Roberts J.D. Jr., Zapol D.G., Lepore J.J., Filippov G., Thomas J.E., Jacob H.J., Bloch D.B.
    Genomics 27:526-530(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 987-1122.
    Tissue: Pulmonary artery.

Entry informationi

Entry nameiNOS2_BOVIN
AccessioniPrimary (citable) accession number: Q27995
Secondary accession number(s): Q06Q84, Q27985
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 28, 2006
Last modified: November 26, 2014
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3