Reviewed,
UniProtKB/Swiss-Prot Q27995 (NOS2_BOVIN)
Last modified
November 25, 2008.
Version 66.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Nitric oxide synthase, inducible EC=1.14.13.39 Alternative name(s): Inducible NO synthase Short name=Inducible NOS Short name=iNOS NOS type II Short name=NOSII | ||
| Gene names |
| ||
| Organism | Bos taurus (Bovine) | ||
| Taxonomic identifier | 9913 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Protein attributes
| Sequence length | 1156 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. |
| Catalytic activity | L-arginine + n NADPH + m O(2) = citrulline + nitric oxide + n NADP(+). |
| Cofactor | Heme group By similarity. Binds 1 FAD By similarity. Binds 1 FMN By similarity. Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity. |
| Enzyme regulation | Regulated by calcium/calmodulin By similarity. |
| Subunit structure | Homodimer. Binds SLC9A3R1 By similarity. |
| Sequence similarities | Belongs to the NOS family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain. |
Ontologies
Keywords | |
|---|---|
| Ligand | Calcium Calmodulin-binding FAD FMN Heme Iron Metal-binding NADP |
| Molecular function | Oxidoreductase |
Gene Ontology (GO) | |
| Biological process | nitric oxide biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | FAD binding Inferred from electronic annotation. Source: InterPro FMN bindingInferred from electronic annotation. Source: InterPro NADP bindingInferred from electronic annotation. Source: InterPro calcium ion bindingInferred from electronic annotation. Source: UniProtKB-KW calmodulin bindingInferred from electronic annotation. Source: InterPro electron carrier activityInferred from electronic annotation. Source: InterPro heme bindingInferred from electronic annotation. Source: InterPro iron ion bindingInferred from electronic annotation. Source: InterPro nitric-oxide synthase activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1156 | 1156 | Nitric oxide synthase, inducible | PRO_0000170926 | |||||
Regions | |||||||||
| Domain | 539 – 677 | 139 | Flavodoxin-like | ||||||
| Domain | 730 – 970 | 241 | FAD-binding FR-type | ||||||
| Nucleotide binding | 623 – 654 | 32 | FMN By similarity | ||||||
| Nucleotide binding | 767 – 778 | 12 | FAD By similarity | ||||||
| Nucleotide binding | 903 – 913 | 11 | FAD By similarity | ||||||
| Nucleotide binding | 978 – 996 | 19 | NADP By similarity | ||||||
| Nucleotide binding | 1076 – 1091 | 16 | NADP By similarity | ||||||
| Region | 509 – 529 | 21 | Calmodulin-binding Potential | ||||||
Sites | |||||||||
| Metal binding | 200 | 1 | Iron (heme axial ligand) By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 236 | 1 | N → S in AAC48470. Ref.2 | ||||||
| Sequence conflict | 1068 – 1069 | 2 | VL → AF in AAC48479. Ref.3 | ||||||
Sequences
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References
| [1] | "Cloning and characterization of bovine inducible nitric oxide synthase." Widdison S., Ashley G.R., Howard C.J., Coffey T.J. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Inducible nitric oxide synthase in cattle. Differential cytokine regulation of nitric oxide synthase in bovine and murine macrophages." Adler H., Frech B., Thoeny M., Pfister H., Peterhans E., Jungi T.W. J. Immunol. 154:4710-4718(1995) [PubMed: 7536776] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 166-275. Tissue: Bone marrow macrophage. |
| [3] | "Three members of the nitric oxide synthase II gene family (NOS2A, NOS2B, and NOS2C) colocalize to human chromosome 17." Bloch K.D., Wolfram J.R., Brown D.M., Roberts J.D. Jr., Zapol D.G., Lepore J.J., Filippov G., Thomas J.E., Jacob H.J., Bloch D.B. Genomics 27:526-530(1995) [PubMed: 7558036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 987-1122. Tissue: Pulmonary artery. |
Cross-references
Sequence databases | |
|---|---|
| DQ676956 mRNA. Translation: ABG74910.1. U14640 mRNA. Translation: AAC48470.2. U18331 mRNA. Translation: AAC48479.1. | |
| PIR | I46074. |
| RefSeq | NP_001070267.1. |
| UniGene | Bt.23126 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1DD7 based on UniProtKB P29477. |
| SMR | Q27995. Positions 83-502. |
| ModBase | Search... |
Genome annotation databases | |
| Ensembl | ENSBTAG00000006894. Bos taurus. [Contig view] |
| GeneID | 282876. |
| KEGG | bta:282876. |
Phylogenomic databases | |
| HOVERGEN | Q27995. |
Family and domain databases | |
| InterPro | IPR003097. FAD-binding_1. IPR001094. Flavdoxin_like. IPR008254. Flavodoxin/NO_synth. IPR001709. FPN_cyt_redctse. IPR004030. NO_synthase_oxygenase_reg. IPR012144. NOS. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view] |
| Pfam | PF00667. FAD_binding_1. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF00175. NAD_binding_1. 1 hit. PF02898. NO_synthase. 1 hit. [Graphical view] |
| PIRSF | PIRSF000333. NOS. 1 hit. |
| PRINTS | PR00369. FLAVODOXIN. PR00371. FPNCR. |
| PROSITE | PS51384. FAD_FR. 1 hit. PS50902. FLAVODOXIN_LIKE. 1 hit. PS60001. NOS. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | NOS2_BOVIN | ||||||||
| Accession | Primary (citable) accession number: Q27995 Secondary accession number(s): Q06Q84, Q27985 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| UniProtKB secondary accession numbers Index of UniProtKB secondary accession numbers |
| SIMILARITY comments Index of protein domains and families |

Clusters with


