Reviewed, UniProtKB/Swiss-Prot Q27995 (NOS2_BOVIN)

Names and origin

Protein names

Recommended name:
    Nitric oxide synthase, inducible
    EC=1.14.13.39
Alternative name(s):
    Inducible NO synthase
      Short name=Inducible NOS
      Short name=iNOS
    NOS type II
      Short name=NOSII

Gene names

Name:

NOS2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	1156 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.
Catalytic activity	L-arginine + n NADPH + m O(2) = citrulline + nitric oxide + n NADP(+).
Cofactor	Heme group By similarity. Binds 1 FAD By similarity. Binds 1 FMN By similarity. Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.
Enzyme regulation	Regulated by calcium/calmodulin By similarity.
Subunit structure	Homodimer. Binds SLC9A3R1 By similarity.
Sequence similarities	Belongs to the NOS family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain.

Ontologies

Keywords
Ligand	Calcium Calmodulin-binding FAD FMN Heme Iron Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	nitric oxide biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro FMN binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro nitric-oxide synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1156	1156	Nitric oxide synthase, inducible		PRO_0000170926
Regions
	Domain	539 – 677	139	Flavodoxin-like
	Domain	730 – 970	241	FAD-binding FR-type
	Nucleotide binding	623 – 654	32	FMN By similarity
	Nucleotide binding	767 – 778	12	FAD By similarity
	Nucleotide binding	903 – 913	11	FAD By similarity
	Nucleotide binding	978 – 996	19	NADP By similarity
	Nucleotide binding	1076 – 1091	16	NADP By similarity
	Region	509 – 529	21	Calmodulin-binding Potential
Sites
	Metal binding	200	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	236	1	N → S in AAC48470. Ref.2
	Sequence conflict	1068 – 1069	2	VL → AF in AAC48479. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q27995-1 [UniParc]. Last modified November 28, 2006. Version 3. Checksum: 52A52C984462F692	FASTA	1,156	131,208
	10 20 30 40 50 60 MACPWQFLFK IKSQKVDLAT ELDINNNVGK FYQPPSSPVT QDDPKRHSPG KHGNESPQPL 70 80 90 100 110 120 TGTVKTSPES LSKLDAPPSA CPRHVRIKNW GSGVTFQDTL HQKAKGDLSC KSKSCLASIM 130 140 150 160 170 180 NPKSLTIGPR DKPTPPDELL PQAIEFVNQY YGSFKEAKIE EHLARVEAVT KEIETTGTYQ 190 200 210 220 230 240 LTGDELIFAT KQAWRNAPRC IGRIQWSNLQ VFDARSCSTA QEMFEHICRH VRYATNNGNI 250 260 270 280 290 300 RSAITVFPQR SDGKHDFRVW NAQLIRYAGY QMPDGSIRGD PANVEFTQLC IDLGWKPKYG 310 320 330 340 350 360 RFDVLPLVLQ ADGRDPELFE IPPDLVLEVP MEHPRYEWFR ELELKWYALP AVANMLLEVG 370 380 390 400 410 420 GLEFPGCPFN GWYMGTEVGV RDFCDAQRYN ILEEVGRRMG LETHKVASLW KDRAVVEINV 430 440 450 460 470 480 AVLHSFQKQN VTIMDHHSAA ESFMKYMQNE YRSRGGCPAD WIWLVPPISG SITPVFHQEM 490 500 510 520 530 540 LNYVLSPFYY YQVEPWKTHV WQDERRRPQR REIRFKVLVK AVFFASVLMH KAMASRVRAT 550 560 570 580 590 600 ILFATETGRS ETLAQDLGAL FSCAFNPKVL CMDQYQLSHL EEEQLLLVVT STFGNGDSPG 610 620 630 640 650 660 NGEKLKKSLL MLKELTNTFR YAVFGLGSSM YPQFCAFAHD IDQKLSQLGA SQLAPTGEGD 670 680 690 700 710 720 ELSGQEEAFR SWAVQTFKAA CETFDVSGKH HIEIPKLYTS NVTWDPQHYR LVQDSEPLDL 730 740 750 760 770 780 NKALSSMHAK HVFTMRLKSQ QNLQSPKSSR TTLLVELSCE GSQAPSYLPG EHLGVFPCNQ 790 800 810 820 830 840 PALVQGILER VVDGPAPHQP VRLETLCENG SYWVKDKRLP PCSLSQALTY FLDITTPPTQ 850 860 870 880 890 900 LLLRKLAQLA TEEAEKQRLE TLCQPSDYNK WKFTNSPTFL EVLEEFPSLR VSASFLLSQL 910 920 930 940 950 960 PILKPRYYSI SSSRDLTPTE IHLTVAVLTY RTRDGQGPLH HGVCSTWLSS LKPQDPVPCF 970 980 990 1000 1010 1020 VRSASGFQLP EDRSRPCILI GPGTGIAPFR SFWQQRLHEA EHKGLQGGRM TLVFGCRRPE 1030 1040 1050 1060 1070 1080 EDHLYWEEML EMARKGVLHE VHTAYSRLPD QPKVYVQDIL RQRLAGEVLR VLHEEQGHLY 1090 1100 1110 1120 1130 1140 VCGDVRMARD VARTLKQLMA TALSLNEEQV EDYFFQLKNQ KRYHEDIFGA VFPYEVKKDG 1150 AAGLPSNPRA PGAHRS

References

[1]	"Cloning and characterization of bovine inducible nitric oxide synthase." Widdison S., Ashley G.R., Howard C.J., Coffey T.J. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Inducible nitric oxide synthase in cattle. Differential cytokine regulation of nitric oxide synthase in bovine and murine macrophages." Adler H., Frech B., Thoeny M., Pfister H., Peterhans E., Jungi T.W. J. Immunol. 154:4710-4718(1995) [PubMed: 7536776] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 166-275. Tissue: Bone marrow macrophage.
[3]	"Three members of the nitric oxide synthase II gene family (NOS2A, NOS2B, and NOS2C) colocalize to human chromosome 17." Bloch K.D., Wolfram J.R., Brown D.M., Roberts J.D. Jr., Zapol D.G., Lepore J.J., Filippov G., Thomas J.E., Jacob H.J., Bloch D.B. Genomics 27:526-530(1995) [PubMed: 7558036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 987-1122. Tissue: Pulmonary artery.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ676956 mRNA. Translation: ABG74910.1. U14640 mRNA. Translation: AAC48470.2. U18331 mRNA. Translation: AAC48479.1.
PIR	I46074.
RefSeq	NP_001070267.1.
UniGene	Bt.23126
3D structure databases
HSSP	HSSP built from PDB template 1DD7 based on UniProtKB P29477.
SMR	Q27995. Positions 83-502.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000006894. Bos taurus. [Contig view]
GeneID	282876.
KEGG	bta:282876.
Phylogenomic databases
HOVERGEN	Q27995.
Family and domain databases
InterPro	IPR003097. FAD-binding_1. IPR001094. Flavdoxin_like. IPR008254. Flavodoxin/NO_synth. IPR001709. FPN_cyt_redctse. IPR004030. NO_synthase_oxygenase_reg. IPR012144. NOS. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Pfam	PF00667. FAD_binding_1. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF00175. NAD_binding_1. 1 hit. PF02898. NO_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000333. NOS. 1 hit.
PRINTS	PR00369. FLAVODOXIN. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. PS50902. FLAVODOXIN_LIKE. 1 hit. PS60001. NOS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NOS2_BOVIN

Accession

Primary (citable) accession number: Q27995
Secondary accession number(s): Q06Q84, Q27985

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 28, 2006
Last modified:	November 25, 2008
This is version 66 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)