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Q27995

- NOS2_BOVIN

UniProt

Q27995 - NOS2_BOVIN

Protein

Nitric oxide synthase, inducible

Gene

NOS2

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 3 (28 Nov 2006)
      Previous versions | rss
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    Functioni

    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 By similarity.By similarity

    Catalytic activityi

    2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

    Cofactori

    Heme group.By similarity
    Binds 1 FAD.By similarity
    Binds 1 FMN.By similarity
    Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.By similarity

    Enzyme regulationi

    Regulated by calcium/calmodulin.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi200 – 2001Iron (heme axial ligand)By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi623 – 65432FMNPROSITE-ProRule annotationAdd
    BLAST
    Nucleotide bindingi767 – 77812FADBy similarityAdd
    BLAST
    Nucleotide bindingi903 – 91311FADBy similarityAdd
    BLAST
    Nucleotide bindingi978 – 99619NADPBy similarityAdd
    BLAST
    Nucleotide bindingi1076 – 109116NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: InterPro
    2. FMN binding Source: InterPro
    3. heme binding Source: InterPro
    4. iron ion binding Source: InterPro
    5. NADP binding Source: InterPro
    6. nitric-oxide synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. nitric oxide biosynthetic process Source: InterPro
    2. peptidyl-cysteine S-nitrosylation Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Calcium, Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitric oxide synthase, inducible (EC:1.14.13.39)
    Alternative name(s):
    Inducible NO synthase
    Short name:
    Inducible NOS
    Short name:
    iNOS
    NOS type II
    Short name:
    NOSII
    Peptidyl-cysteine S-nitrosylase NOS2
    Gene namesi
    Name:NOS2
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11561156Nitric oxide synthase, induciblePRO_0000170926Add
    BLAST

    Proteomic databases

    PRIDEiQ27995.

    Interactioni

    Subunit structurei

    Homodimer. Binds SLC9A3R1 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ27995.
    SMRiQ27995. Positions 83-502, 703-1130.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini539 – 677139Flavodoxin-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini730 – 970241FAD-binding FR-typePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni509 – 52921Calmodulin-bindingSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the NOS family.Curated
    Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
    Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG4362.
    HOGENOMiHOG000220884.
    HOVERGENiHBG000159.
    InParanoidiQ27995.
    KOiK13241.

    Family and domain databases

    Gene3Di1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    3.90.340.10. 1 hit.
    InterProiIPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR012144. NOS_euk.
    IPR004030. NOS_N.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    PF02898. NO_synthase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000333. NOS. 1 hit.
    PRINTSiPR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMiSSF52218. SSF52218. 1 hit.
    SSF56512. SSF56512. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    PS60001. NOS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q27995-1 [UniParc]FASTAAdd to Basket

    « Hide

    MACPWQFLFK IKSQKVDLAT ELDINNNVGK FYQPPSSPVT QDDPKRHSPG     50
    KHGNESPQPL TGTVKTSPES LSKLDAPPSA CPRHVRIKNW GSGVTFQDTL 100
    HQKAKGDLSC KSKSCLASIM NPKSLTIGPR DKPTPPDELL PQAIEFVNQY 150
    YGSFKEAKIE EHLARVEAVT KEIETTGTYQ LTGDELIFAT KQAWRNAPRC 200
    IGRIQWSNLQ VFDARSCSTA QEMFEHICRH VRYATNNGNI RSAITVFPQR 250
    SDGKHDFRVW NAQLIRYAGY QMPDGSIRGD PANVEFTQLC IDLGWKPKYG 300
    RFDVLPLVLQ ADGRDPELFE IPPDLVLEVP MEHPRYEWFR ELELKWYALP 350
    AVANMLLEVG GLEFPGCPFN GWYMGTEVGV RDFCDAQRYN ILEEVGRRMG 400
    LETHKVASLW KDRAVVEINV AVLHSFQKQN VTIMDHHSAA ESFMKYMQNE 450
    YRSRGGCPAD WIWLVPPISG SITPVFHQEM LNYVLSPFYY YQVEPWKTHV 500
    WQDERRRPQR REIRFKVLVK AVFFASVLMH KAMASRVRAT ILFATETGRS 550
    ETLAQDLGAL FSCAFNPKVL CMDQYQLSHL EEEQLLLVVT STFGNGDSPG 600
    NGEKLKKSLL MLKELTNTFR YAVFGLGSSM YPQFCAFAHD IDQKLSQLGA 650
    SQLAPTGEGD ELSGQEEAFR SWAVQTFKAA CETFDVSGKH HIEIPKLYTS 700
    NVTWDPQHYR LVQDSEPLDL NKALSSMHAK HVFTMRLKSQ QNLQSPKSSR 750
    TTLLVELSCE GSQAPSYLPG EHLGVFPCNQ PALVQGILER VVDGPAPHQP 800
    VRLETLCENG SYWVKDKRLP PCSLSQALTY FLDITTPPTQ LLLRKLAQLA 850
    TEEAEKQRLE TLCQPSDYNK WKFTNSPTFL EVLEEFPSLR VSASFLLSQL 900
    PILKPRYYSI SSSRDLTPTE IHLTVAVLTY RTRDGQGPLH HGVCSTWLSS 950
    LKPQDPVPCF VRSASGFQLP EDRSRPCILI GPGTGIAPFR SFWQQRLHEA 1000
    EHKGLQGGRM TLVFGCRRPE EDHLYWEEML EMARKGVLHE VHTAYSRLPD 1050
    QPKVYVQDIL RQRLAGEVLR VLHEEQGHLY VCGDVRMARD VARTLKQLMA 1100
    TALSLNEEQV EDYFFQLKNQ KRYHEDIFGA VFPYEVKKDG AAGLPSNPRA 1150
    PGAHRS 1156
    Length:1,156
    Mass (Da):131,208
    Last modified:November 28, 2006 - v3
    Checksum:i52A52C984462F692
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti236 – 2361N → S in AAC48470. (PubMed:7536776)Curated
    Sequence conflicti1068 – 10692VL → AF in AAC48479. (PubMed:7558036)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ676956 mRNA. Translation: ABG74910.1.
    U14640 mRNA. Translation: AAC48470.2.
    U18331 mRNA. Translation: AAC48479.1.
    PIRiI46074.
    RefSeqiNP_001070267.1. NM_001076799.1.
    UniGeneiBt.23126.

    Genome annotation databases

    GeneIDi282876.
    KEGGibta:282876.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ676956 mRNA. Translation: ABG74910.1 .
    U14640 mRNA. Translation: AAC48470.2 .
    U18331 mRNA. Translation: AAC48479.1 .
    PIRi I46074.
    RefSeqi NP_001070267.1. NM_001076799.1.
    UniGenei Bt.23126.

    3D structure databases

    ProteinModelPortali Q27995.
    SMRi Q27995. Positions 83-502, 703-1130.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q27995.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 282876.
    KEGGi bta:282876.

    Organism-specific databases

    CTDi 4843.

    Phylogenomic databases

    eggNOGi COG4362.
    HOGENOMi HOG000220884.
    HOVERGENi HBG000159.
    InParanoidi Q27995.
    KOi K13241.

    Miscellaneous databases

    NextBioi 20806404.

    Family and domain databases

    Gene3Di 1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    3.90.340.10. 1 hit.
    InterProi IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR012144. NOS_euk.
    IPR004030. NOS_N.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view ]
    Pfami PF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    PF02898. NO_synthase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000333. NOS. 1 hit.
    PRINTSi PR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMi SSF52218. SSF52218. 1 hit.
    SSF56512. SSF56512. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEi PS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    PS60001. NOS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of bovine inducible nitric oxide synthase."
      Widdison S., Ashley G.R., Howard C.J., Coffey T.J.
      Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Inducible nitric oxide synthase in cattle. Differential cytokine regulation of nitric oxide synthase in bovine and murine macrophages."
      Adler H., Frech B., Thoeny M., Pfister H., Peterhans E., Jungi T.W.
      J. Immunol. 154:4710-4718(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 166-275.
      Tissue: Bone marrow macrophage.
    3. "Three members of the nitric oxide synthase II gene family (NOS2A, NOS2B, and NOS2C) colocalize to human chromosome 17."
      Bloch K.D., Wolfram J.R., Brown D.M., Roberts J.D. Jr., Zapol D.G., Lepore J.J., Filippov G., Thomas J.E., Jacob H.J., Bloch D.B.
      Genomics 27:526-530(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 987-1122.
      Tissue: Pulmonary artery.

    Entry informationi

    Entry nameiNOS2_BOVIN
    AccessioniPrimary (citable) accession number: Q27995
    Secondary accession number(s): Q06Q84, Q27985
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 28, 2006
    Last modified: October 1, 2014
    This is version 110 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3