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Q27995 (NOS2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitric oxide synthase, inducible

EC=1.14.13.39
Alternative name(s):
Inducible NO synthase
Short name=Inducible NOS
Short name=iNOS
NOS type II
Short name=NOSII
Peptidyl-cysteine S-nitrosylase NOS2
Gene names
Name:NOS2
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length1156 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 By similarity.

Catalytic activity

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor

Heme group By similarity.

Binds 1 FAD By similarity.

Binds 1 FMN By similarity.

Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.

Enzyme regulation

Regulated by calcium/calmodulin By similarity.

Subunit structure

Homodimer. Binds SLC9A3R1 By similarity.

Sequence similarities

Belongs to the NOS family.

Contains 1 FAD-binding FR-type domain.

Contains 1 flavodoxin-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11561156Nitric oxide synthase, inducible
PRO_0000170926

Regions

Domain539 – 677139Flavodoxin-like
Domain730 – 970241FAD-binding FR-type
Nucleotide binding623 – 65432FMN By similarity
Nucleotide binding767 – 77812FAD By similarity
Nucleotide binding903 – 91311FAD By similarity
Nucleotide binding978 – 99619NADP By similarity
Nucleotide binding1076 – 109116NADP By similarity
Region509 – 52921Calmodulin-binding Potential

Sites

Metal binding2001Iron (heme axial ligand) By similarity

Experimental info

Sequence conflict2361N → S in AAC48470. Ref.2
Sequence conflict1068 – 10692VL → AF in AAC48479. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q27995 [UniParc].

Last modified November 28, 2006. Version 3.
Checksum: 52A52C984462F692

FASTA1,156131,208
        10         20         30         40         50         60 
MACPWQFLFK IKSQKVDLAT ELDINNNVGK FYQPPSSPVT QDDPKRHSPG KHGNESPQPL 

        70         80         90        100        110        120 
TGTVKTSPES LSKLDAPPSA CPRHVRIKNW GSGVTFQDTL HQKAKGDLSC KSKSCLASIM 

       130        140        150        160        170        180 
NPKSLTIGPR DKPTPPDELL PQAIEFVNQY YGSFKEAKIE EHLARVEAVT KEIETTGTYQ 

       190        200        210        220        230        240 
LTGDELIFAT KQAWRNAPRC IGRIQWSNLQ VFDARSCSTA QEMFEHICRH VRYATNNGNI 

       250        260        270        280        290        300 
RSAITVFPQR SDGKHDFRVW NAQLIRYAGY QMPDGSIRGD PANVEFTQLC IDLGWKPKYG 

       310        320        330        340        350        360 
RFDVLPLVLQ ADGRDPELFE IPPDLVLEVP MEHPRYEWFR ELELKWYALP AVANMLLEVG 

       370        380        390        400        410        420 
GLEFPGCPFN GWYMGTEVGV RDFCDAQRYN ILEEVGRRMG LETHKVASLW KDRAVVEINV 

       430        440        450        460        470        480 
AVLHSFQKQN VTIMDHHSAA ESFMKYMQNE YRSRGGCPAD WIWLVPPISG SITPVFHQEM 

       490        500        510        520        530        540 
LNYVLSPFYY YQVEPWKTHV WQDERRRPQR REIRFKVLVK AVFFASVLMH KAMASRVRAT 

       550        560        570        580        590        600 
ILFATETGRS ETLAQDLGAL FSCAFNPKVL CMDQYQLSHL EEEQLLLVVT STFGNGDSPG 

       610        620        630        640        650        660 
NGEKLKKSLL MLKELTNTFR YAVFGLGSSM YPQFCAFAHD IDQKLSQLGA SQLAPTGEGD 

       670        680        690        700        710        720 
ELSGQEEAFR SWAVQTFKAA CETFDVSGKH HIEIPKLYTS NVTWDPQHYR LVQDSEPLDL 

       730        740        750        760        770        780 
NKALSSMHAK HVFTMRLKSQ QNLQSPKSSR TTLLVELSCE GSQAPSYLPG EHLGVFPCNQ 

       790        800        810        820        830        840 
PALVQGILER VVDGPAPHQP VRLETLCENG SYWVKDKRLP PCSLSQALTY FLDITTPPTQ 

       850        860        870        880        890        900 
LLLRKLAQLA TEEAEKQRLE TLCQPSDYNK WKFTNSPTFL EVLEEFPSLR VSASFLLSQL 

       910        920        930        940        950        960 
PILKPRYYSI SSSRDLTPTE IHLTVAVLTY RTRDGQGPLH HGVCSTWLSS LKPQDPVPCF 

       970        980        990       1000       1010       1020 
VRSASGFQLP EDRSRPCILI GPGTGIAPFR SFWQQRLHEA EHKGLQGGRM TLVFGCRRPE 

      1030       1040       1050       1060       1070       1080 
EDHLYWEEML EMARKGVLHE VHTAYSRLPD QPKVYVQDIL RQRLAGEVLR VLHEEQGHLY 

      1090       1100       1110       1120       1130       1140 
VCGDVRMARD VARTLKQLMA TALSLNEEQV EDYFFQLKNQ KRYHEDIFGA VFPYEVKKDG 

      1150 
AAGLPSNPRA PGAHRS 

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References

[1]"Cloning and characterization of bovine inducible nitric oxide synthase."
Widdison S., Ashley G.R., Howard C.J., Coffey T.J.
Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Inducible nitric oxide synthase in cattle. Differential cytokine regulation of nitric oxide synthase in bovine and murine macrophages."
Adler H., Frech B., Thoeny M., Pfister H., Peterhans E., Jungi T.W.
J. Immunol. 154:4710-4718(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 166-275.
Tissue: Bone marrow macrophage.
[3]"Three members of the nitric oxide synthase II gene family (NOS2A, NOS2B, and NOS2C) colocalize to human chromosome 17."
Bloch K.D., Wolfram J.R., Brown D.M., Roberts J.D. Jr., Zapol D.G., Lepore J.J., Filippov G., Thomas J.E., Jacob H.J., Bloch D.B.
Genomics 27:526-530(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 987-1122.
Tissue: Pulmonary artery.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ676956 mRNA. Translation: ABG74910.1.
U14640 mRNA. Translation: AAC48470.2.
U18331 mRNA. Translation: AAC48479.1.
PIRI46074.
RefSeqNP_001070267.1. NM_001076799.1.
UniGeneBt.23126.

3D structure databases

ProteinModelPortalQ27995.
SMRQ27995. Positions 83-502, 703-1130.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ27995.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID282876.
KEGGbta:282876.

Organism-specific databases

CTD4843.

Phylogenomic databases

eggNOGCOG4362.
HOGENOMHOG000220884.
HOVERGENHBG000159.
InParanoidQ27995.
KOK13241.

Family and domain databases

Gene3D1.20.990.10. 1 hit.
3.90.340.10. 1 hit.
InterProIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR004030. NO_synthase_oxygenase_dom.
IPR012144. NOS_euk.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PANTHERPTHR19384:SF5. PTHR19384:SF5. 1 hit.
PfamPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF000333. NOS. 1 hit.
PRINTSPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMSSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20806404.

Entry information

Entry nameNOS2_BOVIN
AccessionPrimary (citable) accession number: Q27995
Secondary accession number(s): Q06Q84, Q27985
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 28, 2006
Last modified: January 22, 2014
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families