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Q27995

- NOS2_BOVIN

UniProt

Q27995 - NOS2_BOVIN

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Protein
Nitric oxide synthase, inducible
Gene
NOS2
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 By similarity.

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Heme group By similarity.
Binds 1 FAD By similarity.
Binds 1 FMN By similarity.
Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.

Enzyme regulationi

Regulated by calcium/calmodulin By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi200 – 2001Iron (heme axial ligand) By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi623 – 65432FMN By similarity
Add
BLAST
Nucleotide bindingi767 – 77812FAD By similarity
Add
BLAST
Nucleotide bindingi903 – 91311FAD By similarity
Add
BLAST
Nucleotide bindingi978 – 99619NADP By similarity
Add
BLAST
Nucleotide bindingi1076 – 109116NADP By similarity
Add
BLAST

GO - Molecular functioni

  1. FMN binding Source: InterPro
  2. NADP binding Source: InterPro
  3. flavin adenine dinucleotide binding Source: InterPro
  4. heme binding Source: InterPro
  5. iron ion binding Source: InterPro
  6. nitric-oxide synthase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. nitric oxide biosynthetic process Source: InterPro
  2. peptidyl-cysteine S-nitrosylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calcium, Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase, inducible (EC:1.14.13.39)
Alternative name(s):
Inducible NO synthase
Short name:
Inducible NOS
Short name:
iNOS
NOS type II
Short name:
NOSII
Peptidyl-cysteine S-nitrosylase NOS2
Gene namesi
Name:NOS2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11561156Nitric oxide synthase, inducible
PRO_0000170926Add
BLAST

Proteomic databases

PRIDEiQ27995.

Interactioni

Subunit structurei

Homodimer. Binds SLC9A3R1 By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ27995.
SMRiQ27995. Positions 83-502, 703-1130.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini539 – 677139Flavodoxin-like
Add
BLAST
Domaini730 – 970241FAD-binding FR-type
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni509 – 52921Calmodulin-binding Reviewed prediction
Add
BLAST

Sequence similaritiesi

Belongs to the NOS family.

Phylogenomic databases

eggNOGiCOG4362.
HOGENOMiHOG000220884.
HOVERGENiHBG000159.
InParanoidiQ27995.
KOiK13241.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
3.90.340.10. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000333. NOS. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q27995-1 [UniParc]FASTAAdd to Basket

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MACPWQFLFK IKSQKVDLAT ELDINNNVGK FYQPPSSPVT QDDPKRHSPG     50
KHGNESPQPL TGTVKTSPES LSKLDAPPSA CPRHVRIKNW GSGVTFQDTL 100
HQKAKGDLSC KSKSCLASIM NPKSLTIGPR DKPTPPDELL PQAIEFVNQY 150
YGSFKEAKIE EHLARVEAVT KEIETTGTYQ LTGDELIFAT KQAWRNAPRC 200
IGRIQWSNLQ VFDARSCSTA QEMFEHICRH VRYATNNGNI RSAITVFPQR 250
SDGKHDFRVW NAQLIRYAGY QMPDGSIRGD PANVEFTQLC IDLGWKPKYG 300
RFDVLPLVLQ ADGRDPELFE IPPDLVLEVP MEHPRYEWFR ELELKWYALP 350
AVANMLLEVG GLEFPGCPFN GWYMGTEVGV RDFCDAQRYN ILEEVGRRMG 400
LETHKVASLW KDRAVVEINV AVLHSFQKQN VTIMDHHSAA ESFMKYMQNE 450
YRSRGGCPAD WIWLVPPISG SITPVFHQEM LNYVLSPFYY YQVEPWKTHV 500
WQDERRRPQR REIRFKVLVK AVFFASVLMH KAMASRVRAT ILFATETGRS 550
ETLAQDLGAL FSCAFNPKVL CMDQYQLSHL EEEQLLLVVT STFGNGDSPG 600
NGEKLKKSLL MLKELTNTFR YAVFGLGSSM YPQFCAFAHD IDQKLSQLGA 650
SQLAPTGEGD ELSGQEEAFR SWAVQTFKAA CETFDVSGKH HIEIPKLYTS 700
NVTWDPQHYR LVQDSEPLDL NKALSSMHAK HVFTMRLKSQ QNLQSPKSSR 750
TTLLVELSCE GSQAPSYLPG EHLGVFPCNQ PALVQGILER VVDGPAPHQP 800
VRLETLCENG SYWVKDKRLP PCSLSQALTY FLDITTPPTQ LLLRKLAQLA 850
TEEAEKQRLE TLCQPSDYNK WKFTNSPTFL EVLEEFPSLR VSASFLLSQL 900
PILKPRYYSI SSSRDLTPTE IHLTVAVLTY RTRDGQGPLH HGVCSTWLSS 950
LKPQDPVPCF VRSASGFQLP EDRSRPCILI GPGTGIAPFR SFWQQRLHEA 1000
EHKGLQGGRM TLVFGCRRPE EDHLYWEEML EMARKGVLHE VHTAYSRLPD 1050
QPKVYVQDIL RQRLAGEVLR VLHEEQGHLY VCGDVRMARD VARTLKQLMA 1100
TALSLNEEQV EDYFFQLKNQ KRYHEDIFGA VFPYEVKKDG AAGLPSNPRA 1150
PGAHRS 1156
Length:1,156
Mass (Da):131,208
Last modified:November 28, 2006 - v3
Checksum:i52A52C984462F692
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti236 – 2361N → S in AAC48470. 1 Publication
Sequence conflicti1068 – 10692VL → AF in AAC48479. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ676956 mRNA. Translation: ABG74910.1.
U14640 mRNA. Translation: AAC48470.2.
U18331 mRNA. Translation: AAC48479.1.
PIRiI46074.
RefSeqiNP_001070267.1. NM_001076799.1.
UniGeneiBt.23126.

Genome annotation databases

GeneIDi282876.
KEGGibta:282876.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ676956 mRNA. Translation: ABG74910.1 .
U14640 mRNA. Translation: AAC48470.2 .
U18331 mRNA. Translation: AAC48479.1 .
PIRi I46074.
RefSeqi NP_001070267.1. NM_001076799.1.
UniGenei Bt.23126.

3D structure databases

ProteinModelPortali Q27995.
SMRi Q27995. Positions 83-502, 703-1130.
ModBasei Search...

Proteomic databases

PRIDEi Q27995.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 282876.
KEGGi bta:282876.

Organism-specific databases

CTDi 4843.

Phylogenomic databases

eggNOGi COG4362.
HOGENOMi HOG000220884.
HOVERGENi HBG000159.
InParanoidi Q27995.
KOi K13241.

Miscellaneous databases

NextBioi 20806404.

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
3.90.340.10. 1 hit.
InterProi IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000333. NOS. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of bovine inducible nitric oxide synthase."
    Widdison S., Ashley G.R., Howard C.J., Coffey T.J.
    Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Inducible nitric oxide synthase in cattle. Differential cytokine regulation of nitric oxide synthase in bovine and murine macrophages."
    Adler H., Frech B., Thoeny M., Pfister H., Peterhans E., Jungi T.W.
    J. Immunol. 154:4710-4718(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 166-275.
    Tissue: Bone marrow macrophage.
  3. "Three members of the nitric oxide synthase II gene family (NOS2A, NOS2B, and NOS2C) colocalize to human chromosome 17."
    Bloch K.D., Wolfram J.R., Brown D.M., Roberts J.D. Jr., Zapol D.G., Lepore J.J., Filippov G., Thomas J.E., Jacob H.J., Bloch D.B.
    Genomics 27:526-530(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 987-1122.
    Tissue: Pulmonary artery.

Entry informationi

Entry nameiNOS2_BOVIN
AccessioniPrimary (citable) accession number: Q27995
Secondary accession number(s): Q06Q84, Q27985
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 28, 2006
Last modified: September 3, 2014
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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